scholarly journals Effects of Storage and Exogenous Ga3 on Lychee Seed Germination

2000 ◽  
Vol 43 (4) ◽  
pp. 441-445 ◽  
Author(s):  
Elizabeth Orika Ono ◽  
Sarita Leonel ◽  
Jayme Duarte Filho ◽  
João Domingos Rodrigues
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Storage Time ◽  
Storage Period ◽  
Germination Percentage ◽  
Germination Capacity ◽  
Seeds Germination

The effects of storage time and exogenous gibberellic acid on lychee seeds germination were studied. The seeds were removed from ripe fruits, washed, dried, stored at 8°C for 0, 15 and 30 days, and soaked during 24 hours in water and GA3 at 50, 100 and 200 mg.L-1 solutions. As the storage period increased, the germination capacity was lost, and the seeds had a short germinability after 30-day storage period. Gibberellic acid had not significant effect on enhancing both the germination percentage and rate.

scholarly journals Physical characterization of Rhipsalis (Cactaceae) fruits and seeds germination in different temperatures and light regimes

2016 ◽  
Vol 76 (2) ◽  
pp. 367-373 ◽  
Author(s):  
A. B. Lone ◽  
R. C. Colombo ◽  
B. L. G. Andrade ◽  
L. S. A. Takahashi ◽  
R. T. Faria
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Light Regimes ◽  
Total Absence ◽  
Germination Characteristics ◽  
Different Temperatures ◽  
Medium Time ◽  
Seeds Germination

Abstract The germination characteristics of the native cactus species are poorly known, being the temperature and the light the factors that the most interferes in that process. Thus, the objective of the present work was to characterize the fruits and evaluate the influence of the temperature and the light in the seed germination of Rhipsalis floccosa, Rhipsalis pilocarpa and Rhipsalis teres. The tested constant temperatures were 15, 20, 25, 30 and 35 °C and the alternate of 20-30 °C and 25-35 °C in a photoperiod of 10 hours, and with determination of the most appropriate temperature, the germination was tested in light absence. The germination percentage, the index of germination speed and medium time of germination were evaluated. For R. floccosa, the highest germination percentage was at 20 °C. For R. pilocarpa and R. teres, the highest germination percentages occurred in 15 °C and 20 °C. There was correlation to germination percentage between the three species, indicating that they had similar germination behavior. Total absence of germination was verified for the three species in condition of light absence. In conclusion, the temperature of 20 °C is the most suitable for the seed germination of R. floccosa. For the species R. pilocarpa and R. teres, the temperatures of 15 and 20 °C are the most suitable.

Variation in Piceaglauca seed germination associated with the year of cone collection

1993 ◽  
Vol 23 (7) ◽  
pp. 1306-1313 ◽  
Author(s):  
G.E. Caron ◽  
B.S.P. Wang ◽  
H.O. Schooley
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Storage Period ◽  
White Spruce ◽  
Germination Percentage ◽  
Degree Days ◽  
Seed Maturity ◽  
Individual Trees ◽  
Collection Time

The effects of cone storage period and pregermination treatment on seed maturity and dormancy were compared for cones of white spruce (Piceaglauca (Moench) Voss) collected from individual trees in 1984 and 1988. Seeds were extracted from cones and germinated after 2 or 6 weeks of cone storage in 1984 and after 2, 4, 6, 10, or 14 weeks in 1988. Based on cumulative degree-days, seeds were more mature at collection time in 1988 than in 1984. Seeds from 1984 cones stored for 6 weeks matured during storage, and both germination percentage (GP) and rate of germination (GR) were significantly improved. In contrast, storage up to 14 weeks in 1988 did not increase GP and GR, as seed had attained maturity prior to cone collection. Seed dormancy was present in both 1984 and 1988. Significant improvements in GP and GR were achieved in 1984 with a pregermination treatment even before seed maturity was attained. Prechilling of seed after 6 weeks of cone storage increased GP from 60 to 95% in 1984 and 64 to 89% in 1988.

scholarly journals Effect of Gibberellic Acid (GA3) and Kinetin on Seed Germination of Sesbania sesban L. and Sesbania rostrata L. (Fabaceae)

2020 ◽  
pp. 32-41
Author(s):  
S. I. Mensah ◽  
C. Ekeke ◽  
N. K. Ibeagi
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Water Absorption ◽  
Glass Container ◽  
Germination Percentage ◽  
International Institute ◽  
Tropical Agriculture ◽  
Percentage Germination ◽  
Seed Coats ◽  
Ibadan Nigeria

We investigated the effects of gibberellic acid (GA3) and kinetin on seed germination of S. sesban L. and S. rostrata L. The matured seeds used for this study were obtained from International Institute for Tropical Agriculture (IITA) Ibadan, Nigeria. The seeds were harvested in 2014 and stored dry in a glass container and kept (15°C) in the refrigerator. The viability of the seeds were determined by floating the intact seeds in water and water uptake (imbibition) was carried out. Four replicates of 20 seeds per replicate were germinated and the seeds observed daily and final count was recorded after 14 days of incubation at 30°C. Intact seeds were soaked in gibberellic acid (GA3) and kinetin for 24h and germination percentage taken. The results from the water absorption demonstrated that the seeds of S. rostrata is more permeable compared to S. sesnban as indicated by higher water absorption of seeds of S. rostrata (70%) to S. sesban (25.4%) after 24hrs of incubation. The anatomy of the seed coats indicated the presence of water and gas impermeable tissues namely cuticle, macrosclereids, osteosclereids and disintegrated parenchyma layer. Generally, 0.1 mM kinetin and GA3 enhanced significant germinations compared to the control with 0% germination for the 14 days period of germination. The percentage germination of seeds of S. sesban and S. rotrata subjected to different treatments and germinated in 0.1 mM GA3 and water showed a progressive decrease in germination. From our study, 0.1 mM GA3 and Kinetin significantly enhanced seed germination of S. sesban and S. rostrata.

scholarly journals GERMINATION OF STORED SEEDS OF FOUR TREE SPECIES FROM THE TROPICAL DRY FOREST OF MORELOS, MEXICO

2014 ◽  
Vol 92 (2) ◽  
pp. 281 ◽  
Author(s):  
Martha Cervantes ◽  
Eliane Ceccon ◽  
Consuelo Bonfil
Keyword(s):  
Seed Germination ◽  
Seed Size ◽  
Storage Time ◽  
Germination Rate ◽  
Tropical Dry Forest ◽  
Dry Forest ◽  
Tropical Dry Forests ◽  
Germination Capacity ◽  
Dry Forests ◽  
And Storage

<p><span style="font-size: medium;"><span style="font-family: Times New Roman;">Studies on propagation of trees of Tropical Dry Forests are scarce in Mexico, besides, the provenance of seeds used in reforestation programs is generally unknown or poorly addressed. Knowledge on seed germination patterns of different provenances, and how they change through time, is useful to identify adequate sources of seeds and to develop seed collection and storage programs under the low-tech conditions prevailing in most rural nurseries. We evaluated seed size variation and germination of stored seeds from three different provenances per species in <em>Acacia bilimekii</em>, <em>Haematoxylum brasiletto</em>, <em>Lysiloma acapulcense</em>, and<em> L. divaricatum</em>.<em> </em>Seeds were collected in four sites in the Tropical Dry Forest of Morelos, Mexico, and were stored at room temperature; seed size was estimated through the volume of 75 seeds per provenance/species. Seed germination tests were made periodically from six to 24 months after storage and the effects of provenance and storage time on germination were analyzed using Anovas. There were significant differences in seed size among provenances in all species, while the effect of provenance on germination rate was significant in three of them. Germination rate changed with storage time among species and provenances. After 24 months, germination capacity was still ≥ 50% in all provenances of the two <em>Lysiloma</em> species, but in <em>A.</em> <em>bilimekii</em> there were large differences among provenances. Mean germination capacity was low in <em>H. brasiletto</em> after six months. More research on seed germination and storage of a larger set of species and provenances is needed to restore the Mexican tropical dry forests. </span></span></p>

scholarly journals Pengaruh Media Dan Periode Simpan Terhadap Viabilitas Benih Cengkeh Tuni (Syzygium aromaticum L.)

2018 ◽  
Vol 14 (2) ◽  
pp. 97-104
Author(s):  
Marselina Noya ◽  
Johan Riry ◽  
Marthini Lesilolo
Keyword(s):  
Storage Time ◽  
Germination Rate ◽  
Storage Period ◽  
Germination Percentage ◽  
Syzygium Aromaticum ◽  
Storage Media ◽  
Randomized Design ◽  
Vigor Index ◽  
The Media ◽  
And Storage

This study aims to examine the influence of the media and the storage period on the viability of clove seeds. The study was conducted at the Ambon Plant Crops Protection and Protection Center laboratory. The study used a factorial completely randomized design with two types of factors and three replications. The storage media consists of 3 levels, namely: Without the storage media, the media for storing rice husk and the media for storing sawdust. The second factor is the storage period consists of 4 levels, namely: 10 days storage, 20 days storage, 30 days storage and 40 days storage. The parameters measured were germination power, germination rate, and vigor index. Storage media and storage period significantly affected germination and vigor indexes but did not significantly affect germination rates. The results showed that the sawdust storage media was a good medium for storing clove seeds (Syzygium aromaticum L.). This can be seen in the results of the average germination of clove seeds stored in powder storage media with a storage time of 10 days giving better results of 85% compared to rice husks at a storage time of 10 days with a germination percentage of 65%.  Keywords: clove seeds, save media, save period   ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh media dan periode simpan terhadap viabilitas benih cengkeh. Penelitian dilaksanakan di laboratorium Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan Ambon. Penelitian menggunakan rancangan acak lengkap pola faktorial dengan dua macam faktor dan tiga kali ulangan. Media simpan terdiri dari 3 taraf yaitu: Tanpa media simpan, media simpan sekam padi dan media simpan serbuk gergaji. Faktor kedua adalah periode simpan terdiri dari 4 taraf yaitu: penyimpanan 10 hari, penyimpanan 20 hari, penyimpanan 30 hari dan penyimpanan 40 hari. Parameter yang diukur adalah daya berkecambah, laju perkecambahan dan indeks vigor. Media simpan dan periode penyimpanan berpengaruh nyata pada persentase perkecambahan dan indeks vigor tetapi tidak berpengaruh nyata pada laju perkecambahan. Hasil penelitian menunjukan bahwa media simpan serbuk gergaji merupakan media yang baik bagi penyimpanan benih cengkeh (Syzygium aromaticum L.). Hal ini terlihat pada hasil rata-rata daya berkecambah benih cengkeh yang disimpan pada media simpan serbuk dengan lama penyimpanan selama 10 hari memberikan hasil yang lebih baik yakni 85% jika dibandingkan dengan sekam padi pada lama penyimpanan 10 hari dengan persentase perkecambahan 65%. Kata Kunci: benih cengkeh, media simpan, periode simpan

scholarly journals Storage Period and Different Abiotic Factors Regulate Seed Germination of Two Apocynum Species — Cash Crops in Arid Saline Regions in the Northwestern China

2021 ◽  
Vol 12 ◽  
Author(s):  
Li Jiang ◽  
Chaowen She ◽  
Changyan Tian ◽  
Mohsin Tanveer ◽  
Lei Wang
Keyword(s):  
Seed Germination ◽  
Abiotic Stresses ◽  
Arid Regions ◽  
Natural Fiber ◽  
Abiotic Factors ◽  
Light Condition ◽  
Storage Period ◽  
Germination Percentage ◽  
Environmental Benefits ◽  
Degraded Land

On degraded land in arid regions, cultivation of Apocynum species can provide significant environmental benefits by preventing soil erosion and desertification. Furthermore, Apocynum venetum and Apocynum pictum, which are mainly distributed in salt-barren lands in the northwestern region of China, are traditionally used to produce natural fiber and herbal tea. Direct sowing of both species may encounter various abiotic stresses such as drought and salinity. However, these effects on germination remain largely unknown, especially for seeds with different storage periods. The aim of this study was to evaluate the effects of storage period, light condition, temperature regime, drought, and salinity on germination performances of both species. Germination experiment was carried out in November 2017. There were four replicates for each treatment, and each petri dish contained 25 seeds. The results indicated that prolongation of storage period significantly decreased the germination percentage and velocity, especially under abiotic stresses. Light did not affect seed germination of A. venetum and A. pictum under any conditions. Seeds had better germination performance at 10/25 and 15/30°C than those of seeds incubated at any other temperatures. With the increase of polyethylene glycol (PEG) and salinity concentrations, seed germination for both species gradually decreased, especially for seeds stored for 2 years. Low PEG (0–20%) and salinity concentration (0–200 mM) did not significantly affect germination percentage of freshly matured seeds. However, long-time storage significantly decreased drought and salinity tolerance in A. venetum and A. pictum during germination stage. For saline soils in arid and semi-arid regions, freshly matured seeds or 1-year-stored seeds of both Apocynum species are recommended to be sown by using drip-irrigation in spring.

scholarly journals PENGARUH WADAH, SUHU DAN WAKTU SIMPAN TERHADAP PERKECAMBAHAN BENIH MENIRAN (Phyllanthus niruri L.)

2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Nurul Husniyati Listyana ◽  
Rahma Widyastuti ◽  
Widyantoro Widyantoro
Keyword(s):  
Seed Germination ◽  
Storage Time ◽  
Storage Temperature ◽  
Storage Period ◽  
Raw Material ◽  
Wild Plant ◽  
Phyllanthus Niruri ◽  
Storage Container ◽  
Genetic Traits ◽  
And Storage

ABSTRACT Meniran (Phyllanthus niruri L.) is a wild plant that used extensively as a raw material for traditional medicine but has not been cultivated yet. Meniran cultivation requires the availability of quality seeds and seedlings to produce the high productivity. The ability of seeds to germinate (seed viability) after the storage period is influenced by several factors both internal and external, including seed water content, genetic traits, initial viability and packaging material, storage temperature and humidity, microorganisms, and human factors.. The study aims to determine the effect of storage containers, storage temperature and time of storage on the power of meniran seed germination. The study was conducted in March-April 2018 at the B2P2TO2T Seed Laboratory. The research design was Factorial Completely Randomized Design with 3 factors and 3 replications. The first factor was a storage container consisting of plastic, paper envelopes and aluminum foil. Second factor was a storage temperature consisting of 0 ° C, -20 ° C and -50 ° C.  The third factor was storage time which consists of 2 weeks, 4 weeks, 6 weeks, 8 weeks and 10 weeks. The results showed that the storage container and storage time had a significantly different effect on meniran seed germination. The storage time does not have a significant effect on germination. Together, the storage container, storage temperature and storage time do not have a significant effect on meniran seed germination. The interaction between the storage temperature and the storage time and the interaction between the storage container and the storage time give significantly different effects on germination. ABSTRAK Meniran (Phyllanthus niruri L.) merupakan tanaman liar yang simplisianya banyak dibutuhkan sebagai bahan baku obat tradisional namun belum dibudidayakan. Budidaya tanaman meniran memerlukan ketersediaan benih dan bibit yang bermutu untuk menghasilkan produktivitas yang tinggi. Kemampuan benih untuk berkecambah (viabilitas benih) setelah masa penyimpanan dipengaruhi oleh beberapa faktor baik internal maupun eksternal, meliputi kadar air benih, sifat genetik, viabilitas awal serta bahan pengemas, suhu dan kelembaban ruang simpan, mikroorganisme, serta faktor manusia. Penelitian bertujuan untuk mengetahui pengaruh wadah simpan, suhu simpan dan waktu simpan terhadap daya perkecambahan benih meniran. Penelitian dilaksanakan pada bulan Maret-April 2018 di Laboratorium Benih B2P2TO2T. Desain penelitian yang digunakan yaitu Rancangan Acak Lengkap Faktorial dengan 3 faktor dan 3 ulangan. Faktor 1 yaitu wadah penyimpanan yang terdiri dari plastik, kertas amplop dan aluminium foil. Faktor 2 yaitu suhu simpan yang terdiri dari 0°C, -20°C dan -50°C. Faktor 3 yaitu waktu penyimpanan yang terdiri dari 2 minggu, 4 minggu, 6 minggu, 8 minggu dan 10 minggu. Hasil penelitian menunjukkan wadah simpan dan waktu simpan memberikan pengaruh yang berbeda signifikan terhadap perkecambahan benih meniran.  Suhu simpan tidak memberikan pengaruh yang berbeda signifikan terhadap perkecambahan. Secara bersama-sama wadah simpan, suhu simpan dan waktu simpan tidak memberikan pengaruh yang berbeda signifikan terhadap perkecambahan benih meniran. Interaksi antara suhu simpan dan waktu simpan serta interaksi antara wadah simpan dan waktu simpan memberikan pengaruh yang berbeda signifikan terhadap perkecambahan.  

scholarly journals Experimental seed germination for ex situ conservation of Mexican Podostemaceae

2019 ◽  
Vol 97 (3) ◽  
pp. 413 ◽  
Author(s):  
Valeria Flores-Enríquez ◽  
Guillermo Castillo ◽  
Margarita Collazo-Ortega
Keyword(s):  
Seed Germination ◽  
Storage Time ◽  
Light Quality ◽  
Germplasm Conservation ◽  
Seed Storage ◽  
Germination Percentage ◽  
Seed Banks ◽  
Ex Situ ◽  
Effect Of Temperature ◽  
Collection Site

<p><strong>Background</strong>: Podostemaceae are extremely susceptible to local extinction by habitat loss. Since ~70 % of the river systems in Mexico show some degree of water contamination, it is relevant to generate information about seed storage and germination behavior to design germplasm conservation strategies (<em>e.g</em>., <em>ex situ</em> seed banks) of Mexican podostemads.</p><p><strong>Hypotheses</strong>: Seed germination decreases as seed storage time increases. Further, light quality, temperature and collection site influence similarly the germination response of both species.</p><p><strong>Studied species</strong>: <em>Marathrum foeniculaceum</em> Humb. &amp; Bonpl., <em>Noveloa coulteriana</em> (Tul.)<em> </em>C. Philbrick</p><p><strong>Study site and years of study</strong>: 13 different seeds collections (1996-2013), at four locations in the rivers Horcones and Arroyo del Rincon Jalisco, México. </p><p><strong>Methods</strong>: A germination chamber experiment was performed to evaluate the effect of temperature, light quality and storage time on the germination of both species.</p><p><strong>Results</strong>: Seeds lose viability after nine years of storage. In both species, the time to reach the highest Accumulated Germination Percentage (AGP) was faster in seeds of one-two yr compared to seeds of six-seven yr. <em>N. coulteriana</em> have significant differences in Final Germination Percentage (FGP) between collection rivers. Storage time affects AGP of <em>N. </em>coulteriana more than in <em>M. foeniculaceum.</em></p><p><strong>Conclusions</strong>: Both species can form <em>ex situ</em> seed banks up to 8 yr age in paper bags storage. <em>N. coulteriana</em> is more susceptible to variation in storage conditions compared to <em>M. </em><em>foeniculaceum</em>. Collection site affect seed germination after storing.  Long-term conventional <em>ex situ</em> seed germination storage is not a viable strategy to conserve germplasm of Podostemaceae.</p>

scholarly journals Effects of temperature, gibberellic acid, and KNO3 treatments on seed germination of the wild plant Maesa japonica

2020 ◽  
Vol 48 (1) ◽  
pp. 65-72
Author(s):  
Hyuk Joon Kwon ◽  
So Lim Shin ◽  
Yu-Ri Kim ◽  
Soo-Young Kim
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Morphological Characteristics ◽  
Germination Percentage ◽  
Wild Plant ◽  
Optimal Conditions ◽  
Optimum Temperature ◽  
Dark Cycle ◽  
Germination Speed ◽  
Effects Of Temperature

We investigated the morphological characteristics of Maesa japonica seeds and the optimal conditions for germination. The seeds were exalbuminous, and their length and width were 0.85 ± 0.09 mm and 0.77 ± 0.05 mm, respectively. We tested germination at 15, 20, 25, 30 and 35°C; the optimum temperature for germination was 30°C (64% germination). To test the effects of gibberellic acid (GA3) and KNO3 on germination, the seeds were soaked in solutions containing 0, 0.2, 0.5, 1.0, 1.5 or 2.0 g GA3 L-1 or 0, 0.05, 0.10, 0.20 or 0.50 g KNO3 L-1 before sowing for germination. KNO3 treatment increased germination percentage and speed. Soaking in GA3 concentrations over 1.0 g L-1 stimulated 100% germination after 13 days. A presoaking treatment that combined 1.0 g GA3 L-1 and 0.2 g KNO3 L-1 promoted germination speed and shortened the time in which half of the seeds germinated. Overall, we determined that the best method for germination of Maesa japonica seeds was pre-soaking in 1.0 g GA3 L-1 and 0.2 g KNO3 L-1 at 4°C in the dark for 24 hours, followed by incubation at 25–30°C in a 16-hour light, 8-hour dark cycle for 10 days.

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