Response of the lung after pneumonectomy in alloxan diabetic rats

In order to determine the response of remaining lung after pneumonectomy during diabetes, female Wistar rats that received an intraperitoneal injection of alloxan (D Group) or saline (C Group) were submitted to lung resection. Six days after alloxan injection (45 mg/kg), diabetes was confirmed. The rats were randomized divided in 3 subgroups: non-operated (NO), sham operation (SO) and left pneumonectomy (PE). Thirty days after the surgery all rats were killed and the weight and volume of the lungs were measured. PE-diabetic rats showed smaller lung weight and volume than PE-non-diabetic animals. The results demonstrated that the compensatory lung growth postpneumonectomy were not observed in diabetic rats.

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Heterogeneity of maximal lobar emptying rates in dogs with compensatory lung growth

Journal of Applied Physiology ◽

10.1152/jappl.1989.67.3.1164 ◽

1989 ◽

Vol 67 (3) ◽

pp. 1164-1170 ◽

Cited By ~ 4

Author(s):

S. N. Mink ◽

S. G. Holtby ◽

D. J. Berenzanski ◽

L. Oppenheimer ◽

N. R. Anthonisen

Keyword(s):

Vital Capacity ◽

Sham Operation ◽

Forced Expiration ◽

Lung Growth ◽

Lung Weight ◽

Reduced Pressure ◽

Lung Volumes ◽

Maximum Expiratory Flow ◽

Expiratory Flow ◽

Left Pneumonectomy

Five dogs underwent left pneumonectomy at 10 wk of age, whereas four littermates underwent a sham operation. At 26 wk of age the postpneumonectomy dogs had total lung vital capacity (VC) and lung weight similar to controls, but maximum expiratory flow was reduced. Pressure capsules were glued to right lower (RLL) and right cardiac (RCL) lobes, and alveolar pressures (PA) were measured during forced expiration. In postpneumonectomy dogs RLL and RCL both emptied more slowly than in control dogs, and emptying was especially delayed in RCL, which underwent the most growth. When both lobes deflated together, PA in RCL and RLL were similar in control dogs, but in postpneumonectomy dogs PA in RCL exceeded that in RLL by approximately 3 cmH2O from 80 to 20% VC. Because the higher driving pressure in RCL compensated for the relatively high resistance of RCL, the pattern of lobar emptying was relatively uniform over these lung volumes. This result was compatible with interdependence of lobar maximum expiratory flows. In addition, at PA of 6–10 cmH2O in postpneumonectomy dogs, maximum emptying rates of RCL were less when RCL deflated alone than when RCL and RLL emptied together, again demonstrating interdependence of lobar maximum expiratory flow.

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Effects of acetone extract of Cola nitida on brain sodium-potassium adenosine triphosphatase activity and spatial memory in healthy and streptozotocin-induced diabetic female Wistar rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2016-0019 ◽

2018 ◽

Vol 29 (4) ◽

pp. 411-416 ◽

Cited By ~ 3

Author(s):

Aminat Omolola Imam-Fulani ◽

Kamaldeen Olalekan Sanusi ◽

Bamidele Victor Owoyele

Keyword(s):

Atpase Activity ◽

Spatial Memory ◽

Wistar Rats ◽

Saline Group ◽

Diabetic Rats ◽

Acetone Extract ◽

Diabetic Group ◽

Normal Saline Group ◽

Group 4 ◽

Female Wistar Rats

Abstract Background This study was carried out to investigate the effects of acetone extract of Cola nitida on brain Na+/K+-ATPase activity and spatial memory of healthy and streptozotocin (STZ)-induced diabetic female Wistar rats. Methods Forty-two female Wistar rats were used for this study and were randomly distributed into six groups (n=7). Rats in group 1 were used as control and were administered normal saline; group 2 rats were healthy rats administered 50 mg/kg of kola nut extract per day; group 3 rats were healthy rats administered 100 mg/kg of kola nut extract per day; group 4 rats were a diabetic group also administered normal saline; group 5 rats were diabetic rats administered 50 mg/kg of kola nut extract per day; and group 6 rats were diabetic rats administered 100 mg/kg of kola nut extract per day. Diabetes was induced with 50 mg/kg of STZ. After 3 weeks of administration, the spatial memories of the rats were tested using the Y-maze, followed by assay of Na+/K+-ATPase activity. Results The result shows a significant increase in Na+/K+-ATPase activity of diabetic treated groups (5 and 6) when compared with the diabetic group (4) and a significant increase in Na+/K+-ATPase activity of healthy treated groups (2 and 3) when compared with control. Also, there was a significant increase in spatial memory of the diabetic treated groups when compared with diabetic group. Conclusions This study revealed that kola nut extract has restorative effect on brain Na+/K+-ATPase activities and spatial memory of STZ-induced diabetic female Wistar rats.

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Early Increase in Glomerular Leucocyte Number after a Reducton in Renal Mass: Implications for the Pathogenesis of Glomerulosclerosis

Clinical Science ◽

10.1042/cs0850027 ◽

1993 ◽

Vol 85 (1) ◽

pp. 27-31 ◽

Cited By ~ 7

Author(s):

K. P. G. Harris ◽

F. Baker ◽

J. Brown ◽

J. Walls

Keyword(s):

Wistar Rats ◽

Renal Scarring ◽

Early Response ◽

Sham Operation ◽

Cortical Tissue ◽

Mouse Immunoglobulin ◽

Early Increase ◽

Female Wistar Rats ◽

Glomerular Size

1. Adult female Wistar rats underwent uninephrectomy (n = 8) through a flank incision, or a sham operation (n = 7). One to two weeks later the kidney was perfused in situ and glomeruli were isolated from cortical tissue by sequential sieving, and partially digested. Glomerular leucocytes were labelled with a mouse monoclonal antibody against leucocyte common antigen followed by a fluorescein-labelled anti-mouse immunoglobulin to allow counting. 2. In a further group of animals 24 h albumin excretion and glomerular size were measured 2 weeks after either uninephrectomy (n = 6) or sham operation (n = 6). 3. Glomerular leucocyte number was significantly increased in uninephrectomized animals (15.7 ± 0.9 versus 8.9 ± 0.4, P <0.001), with some glomeruli having leucocyte numbers comparable with those seen in glomerulonephritis. 4. Albuminuria was not increased 2 weeks after uninephrectomy (233 ± 35 versus 170 ± 42 μg/24 h, not significant), and glomerular size was unchanged. Light microscopical appearance was normal. 5. An increase in glomerular leucocyte number is an early response in what was previously considered a non-immunological lesion. It precedes the development of renal scarring and may be important in the pathogenesis of this process.

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Putative Anti-Immobility Action of Acute Insulin Is Attributable to an Increase in Locomotor Activity in Healthy Wistar Rats

Neuropsychobiology ◽

10.1159/000515141 ◽

2021 ◽

pp. 1-10

Author(s):

Ana G. Gutiérrez-García ◽

Carlos M. Contreras

Keyword(s):

Locomotor Activity ◽

Treatment Regimen ◽

Wistar Rats ◽

Day Treatment ◽

Forced Swim Test ◽

Diabetic Rats ◽

Term Treatment ◽

Long Term Treatment ◽

Immobility Time ◽

Female Wistar Rats

Background/Aims: Anti-immobility actions of insulin in diabetic rats that are subjected to the forced swim test (FST) have been reported. In this test, low doses of antidepressants exert actions after long-term treatment, without affecting locomotor activity in healthy rats. Few studies have compared acute and chronic actions of insulin with antidepressants in healthy rats. Methods: We hypothesized that if insulin exerts a true anti-immobility action, then its effects must be comparable to fluoxetine in both a 1-day treatment regimen and a 21-day treatment regimen in healthy, gonadally intact female Wistar rats. Results: The results showed that low levels of glycemia were produced by all treatments, including fluoxetine, and glycemia was lower in proestrus-estrus than in diestrus-metestrus. None of the treatments or regimens produced actions on indicators of anxiety in the elevated plus maze. Insulin in the 1-day regimen increased the number of crossings and rearings in the open field test and caused a low cumulative immobility time in the FST. These actions disappeared in the 21-day regimen. Compared with the other treatments, fluoxetine treatment alone or combined with insulin produced a longer latency to the first period of immobility and a shorter immobility time in the chronic regimen in the FST, without affecting locomotor activity, and more pronounced actions were observed in proestrus-estrus (i.e., a true anti-immobility effect). Conclusion: These results indicate that insulin does not produce a true antidepressant action in healthy rats. The purported antidepressant effects that were observed were instead attributable to an increase in locomotor activity only in the 1-day regimen.

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Influence of treatment with quercetin on lipid parameters and oxidative stress of pregnant diabetic rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2012-0173 ◽

2013 ◽

Vol 91 (2) ◽

pp. 171-177 ◽

Cited By ~ 14

Author(s):

Camila Pereira Braga ◽

Ana Carolina Momentti ◽

Fernando Barbosa Peixoto ◽

Rafaela de Fátima Ferreira Baptista ◽

Felipe André dos Santos ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Biochemical Parameters ◽

Wistar Rats ◽

Diabetic Rats ◽

Lipid Hydroperoxides ◽

Female Wistar Rats ◽

Lipid Parameters ◽

And Oxidative Stress ◽

Pregnant Diabetic

Among the numerous coadjuvant therapies that could influence the incidence and progression of diabetic complications, antioxidants and flavonoids are currently being tested in clinical trials. We investigated the effect of quercetin on biochemical parameters in streptozotocin-induced (60 mg/kg body mass, by intraperitoneal injection) diabetic rats. A total of 32 female Wistar rats were distributed among 4 groups as follows: control (G1); control treated with quercetin (G2); diabetic (G3); and diabetic treated with quercetin (G4). Quercetin administered to pregnant diabetic rats controlled dyslipidemia and improved lipid profiles in diabetes mellitus, regulated oxidative stress by reducing the generation of lipid hydroperoxides, and increased the activity of the antioxidant enzyme glutathione peroxidase.

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Radiologic evaluation using computed tomography for compensatory lung growth; a prospective comparison with histological data from a large animal model

10.21203/rs.3.rs-498601/v1 ◽

2021 ◽

Author(s):

Keiji Ohata ◽

Toyofumi F. Chen-Yoshikawa ◽

Masatsugu Hamaji ◽

Takeshi Kubo ◽

Tatsuo Nakamura ◽

...

Keyword(s):

Computed Tomography ◽

Lung Resection ◽

Large Animal Model ◽

Large Animal ◽

Lung Growth ◽

Lung Weight ◽

Tissue Volume ◽

Compensatory Lung Growth ◽

Post Surgery ◽

Ct Data

Abstract Background Non-invasive analysis using computed tomography (CT) data may be a promising candidate to evaluate neo-alveolarization in adult lungs following lung resection. This study evaluates and compares the validity of CT analysis with histologic morphometry for compensatory lung growth in a large animal model.Methods We calculated the radiologic tissue volume and the radiologic lung weight from CT data taken at 1, 3, and 6 months post-surgery on 15 male beagle dogs that had a right thoractotomy, bilobectomy, or pneumonectomy (n = 5 in each group). Results were analyzed using one-way ANOVA and were subsequently compared to histologic findings of tissue samples at 6 months post-surgery using Pearson’s correlation.Results An increase in radiologic tissue volume and radiologic lung weight was identified, which was positively correlated with histologic lung parenchymal amounts (correlation coefficient = 0.955 and 0.934, respectively, p < 0.001). Histology of lung specimens at six months post-surgery revealed an increase in the tissue amount in both Bilobectomy and Peumonectomy groups, which was consistent with compensatory lung growth.Conclusion Radiologic tissue volume and radiologic lung weight reflected compensatory lung growth following lung resection. Radiologic assessment using CT data can be a promising clinical modality to evaluate postoperative lung growth.

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ANTI-ESTROGENIC PROPERTIES OF ETHANOLIC LEAF EXTRACT OF BOERHAVIA DIFFUSA ON FEMALE WISTAR RATS.

10.1055/s-0037-1608549 ◽

2017 ◽

Author(s):

C Olajide Omolola ◽

J Okpuzor ◽

I Nnadozie Jane ◽

R Adewole Adeniyi

Keyword(s):

Wistar Rats ◽

Leaf Extract ◽

Female Wistar Rats ◽

Estrogenic Properties ◽

Ethanolic Leaf Extract ◽

Boerhavia Diffusa

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NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

Acta Endocrinologica ◽

10.1530/acta.0.0750569 ◽

1974 ◽

Vol 75 (3) ◽

pp. 569-578 ◽

Cited By ~ 29

Author(s):

G. Buffler ◽

S. Roser

Keyword(s):

Wistar Rats ◽

Venous Blood ◽

Oestrous Cycle ◽

Female Rats ◽

Cycle Duration ◽

Follicular Growth ◽

The Third ◽

Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

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A new method for the experimental induction of secundary biliary cirrhosis in wistar rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502001000200003 ◽

2001 ◽

Vol 16 (2) ◽

pp. 75-81 ◽

Cited By ~ 5

Author(s):

Gracinda De Lourdes Jorge ◽

Luiz Sergio Leonardi ◽

Ilka de Fatima Santana Ferreira Boin ◽

Orlando de Castro e Silva Jr ◽

Cecilia Amelia Fazzio Escanhoela

Keyword(s):

Bile Duct ◽

Morphological Study ◽

Wistar Rats ◽

Hepatic Duct ◽

Control Group ◽

Sham Operation ◽

Biliary Cirrhosis ◽

Technical Failure ◽

Duct Obstruction ◽

Duct Ligation

The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.

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