scholarly journals Blood parasites, total plasma protein and packed cell volume of small wild mammals trapped in three mountain ranges of the Atlantic Forest in Southeastern Brazil

2007 ◽  
Vol 67 (3) ◽  
pp. 531-535 ◽  
Author(s):  
MAML. Silva ◽  
A. Ronconi ◽  
N. Cordeiro ◽  
DEP. Bossi ◽  
HG. Bergallo ◽  
...  
Keyword(s):  
Cell Volume ◽  
Atlantic Forest ◽  
Plasma Protein ◽  
Packed Cell Volume ◽  
Blood Parasites ◽  
Southeastern Brazil ◽  
Total Plasma ◽  
Wild Mammals ◽  
Total Plasma Protein ◽  
Significant Difference

A study of blood parasites in small wild non-flying mammals was undertaken in three areas of the Atlantic Forest in Southeastern Brazil: Serra de Itatiaia, RJ, Serra da Bocaina, SP and Serra da Fartura, SP, from June 1999 to May 2001. A total of 450 animals (15 species) were captured in traps and it was observed in 15.5% of the blood smears the presence of Haemobartonella sp. and Babesia sp. in red blood cells. There was no statistically significant difference between parasited and non-parasited specimens regarding total plasma protein, packed cell volume and body weight, which strongly suggests that these specimens might be parasite reservoirs.

scholarly journals Respons Hematologi dan Kimia Darah Domba Lokal Indonesia Terhadap Stres Transportasi Selama 12 Jam (HAEMATOLOGICAL AND BLOOD BIOCHEMICAL RESPONSES TO 12 HOUR TRANSPORTATION STRESS IN IN LOCAL INDONESIAN SHEEP)

2019 ◽  
Vol 20 (1) ◽  
pp. 48
Author(s):  
Sarmin Sarmin ◽  
Amelia Hana ◽  
Pudji Astuti ◽  
Yuda Heru Febrianto ◽  
Claude Mona Airin
Keyword(s):  
Glucose Metabolism ◽  
Cell Volume ◽  
Plasma Protein ◽  
Packed Cell Volume ◽  
Recovery Period ◽  
Total Plasma ◽  
Biochemical Responses ◽  
Blood Biochemical ◽  
Total Plasma Protein ◽  
Transportation Stress

The objective of this study was to investigate the effect of transport 12 hours on  haematological and some biochemical responses of local Indonesian sheeps.  Ten adults (2.0-2.5 years)  male local Indonesian sheeps  BW adult 30-40 kg were transported by L-300 pickup truck starting at 3:00 pm to 3:00 am on the d 2 without stopping. Jugular blood were collecte-d at 7 am (-8 h pre- transportation as baseline), 15:00  immadiately after (0 h),  19:00 (4 h), 23:00 (8 h) ,  03.00 (12 h ), and at 11.00 d 2 (+8 h)  post-  transportation as recovery period). Under  the conditions of the present study, transport of local Indonesian sheeps significantly increased glucose  during 4 h  transport (P <0.05), increased eosinophil at 0 h (P <0.05) and no significant changed in erythrocytes, haemoglobin , monocytes, neutrophils, lymphocytes, leukocytes,  packed cell volume (PCV), and total plasma protein (TPP) (P> 0.05). Our findings showed that transportation 12 h affected  eosinophil during 0 h and glucose metabolism during 4 h of transportation, but did not any affect on some haematology and dehydration responses in local Indonesian sheeps. 

scholarly journals Diseases of the urinary system of cows: 2- Causative bacteria and Haematological changes.

1999 ◽  
Vol 23 (1) ◽  
pp. 11-21
Author(s):  
S. A.G. Al-Samarrae, ◽  
W. S.T. Al- Azawi
Keyword(s):  
Cell Volume ◽  
Creatinine Level ◽  
Blood Urea Nitrogen ◽  
Plasma Protein ◽  
Packed Cell Volume ◽  
Haemoglobin Concentration ◽  
Urine Samples ◽  
Total Plasma ◽  
Bacterial Cultures ◽  
Total Plasma Protein

Urine samples for bacterial cultures (697) and (604) blood samples for packed cell volume, haemoglobin concentration total plasma protein , blood urea nitrogen and creatinine level were collected farms and veterinary clinics (control) and two cow stations in Al- Latifia and Al Dijala.  The bacterial cultures revealed negative results in the control cows , while bacteria isolated from urine samples in the two stations were similar, and they were Staphylococcus aureus, Corynebacterium renale, haemolytic Escherchia coli . and Corynebacterium pyogenes ( 32, 35,15,8) in Al- Latifia station and (84,136,135,8) in Al-Dijala station respectively  Blood examination showed that packed cell volume, haemoglobin concentration, and total plasma protein showed low levels in Al- Dijala , while blood urea nitrogen and creatinine level showed higher levels in the same coup of cows in comparision with the control and Al Latifia cows.  Infectious pustular vulvovaginitis infected the reproductive system of all cows examined in the Al - Dijala station was behined the 100% bacterial isolate . The numbers of bacterial colonies in cultures from this ststion were more than ( 100000 ) coloni / cc. urine. The prevelance of urinary infection at the age of 3, 4,5,6,7years was 6.25% ; 16.47% , 25.71% , 27.77% and 20.75% respectively and the over all present of bacterial isolates in this study was 36.4%. 

PSIX-13 Activation of cell signaling pathways by galectins in cow blood

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 267-267
Author(s):  
Djaafar M Rehrah ◽  
Mulumebet Worku ◽  
Hamid Ismail
Keyword(s):  
Cell Signaling ◽  
Signaling Pathways ◽  
Plasma Protein ◽  
Group Treatment ◽  
Control Group ◽  
Antibody Array ◽  
Total Plasma ◽  
Total Plasma Protein ◽  
Significant Difference ◽  
Cell Signaling Pathways

Abstract Galectins are part of a conserved family of β-galactoside-binding proteins that contribute to critical biological events during mammalian gestation and increasingly recognized for a possible role in the immune response of the cow. The objective of this study was to evaluate the effect of Galectins on signal transduction and cell activation in cow blood. Whole blood was collected aseptically from the jugular vein of healthy Holstein Friesian dairy cows (N=3). Blood samples (2.5ml) in duplicate were treated with 150µl of the four different type of recombinant galectins (1, 3, 4, and 9) respectively and untreated samples were served as control. The concentration of total plasma protein was determined using the Pierce BCA kit. Protein expression profiling was performed using1,358 antibodies on the Full Moon BioSystems’ Signaling Explorer antibody array covering 20 cell signaling pathways, as recommended by the manufacturer using an Agilent microarray scanner. Data normalization was performed using GeneSpring GX software to generate fold changes in gene expression and then filtered to obtain a list of significantly upregulated and downregulated genes. Features were extracted from protein array images of samples treated with Galectin 1, 3, 4, 9, and untreated sample as a control group. Treatment with all four Galectins increased the concentration of total plasma protein. Average increases due to treatment with Gal1, Gal3, Gal4, and Gal9 were 27%, 10%, 20%, and 14% respectively. ANOVA test showed significance difference among the groups (p &lt; 0.05). Dunnett option was used to compare each of the treated samples to the control group as a baseline. The results also showed that there was significant difference between the control group and any of the treated group (p &lt; 0.05). Distinct signaling pathways are activated in response to Galectin exposure. Further studies are needed to define their regulation and functional impact on cow health.

scholarly journals Hematological profile, total plasma protein and fibrinogen concentrations of clinically healthy adult Campeiro horses

2015 ◽  
Vol 46 (1) ◽  
pp. 144-149 ◽  
Author(s):  
Joandes Henrique Fonteque ◽  
Marcio Luiz Ceccatto ◽  
Renan Menegasso Bagio ◽  
Jackson Schade ◽  
Mere Erika Saito ◽  
...  
Keyword(s):  
Plasma Protein ◽  
Healthy Adult ◽  
Complete Blood Count ◽  
Age Groups ◽  
Total Plasma ◽  
Blood Profile ◽  
Pregnant Females ◽  
Total Plasma Protein ◽  
Significant Difference ◽  
Student’S T

ABSTRACT: This research was performed by a multidisciplinary and interagency team and sought to determine the blood profile, total plasma protein and fibrinogen concentrations of clinically healthy adult Campeiro horses. A total of 138 horses (14 stallions, 74 non-pregnant and 50 pregnant mares) over three years of age from breeders located in the states of Santa Catarina and Rio Grande do Sul were divided into groups according to age, sex and pregnancy status. Statistical analysis of the data was performed using the ANOVA test, Student's T test, and descriptive analyses (P<0.05). There was a significant difference (P<0.05) in the values for MCV, MCH, and total number of eosinophiles when comparing different age groups. There was also a notable difference (P<0.05) in the total plasma protein and total number of eosinophile variables when comparing pregnant females to non-pregnant females. These results show that the Campeiro breed presents certain peculiarities regarding variables in the complete blood count, total plasma protein and fibrinogen compared to the values described by other authors for other breeds. Thus, it is suggested that the values established in this study should be used as benchmarks for interpreting erythrocyte and leukocyte counts when evaluating these variables in Campeiro horses.

scholarly journals Hepatic protein synthesis in the sheep: effect of intake as by use of stable-isotope-labelled glycine, leucine and phenylalanine

1997 ◽  
Vol 77 (2) ◽  
pp. 255-271 ◽  
Author(s):  
A. Connell ◽  
A. G Calder ◽  
S. E Anderson ◽  
G. E Lobley*
Keyword(s):  
Protein Synthesis ◽  
Plasma Protein ◽  
Liver Protein ◽  
Plasma Samples ◽  
Total Plasma ◽  
Plasma Albumin ◽  
Total Plasma Protein ◽  
Significant Difference ◽  
Apolipoprotein B100 ◽  
Hepatic Portal

Rates of protein synthesis for the liver, plasma albumin and total plasma protein were quantified in sheep either offered a supra-maintenance intake or fasted for 3 d. The technique of continuous infusion over a 12 h period was employed with the simultaneous infusion of [1-13C]glycine, [1-13C]leucine and [2H5]phenylalanine. Blood and plasma samples were removed at timed intervals from the hepatic portal and hepatic veins plus the aorta. Enrichments of the free amino acids (AA) were determined in all blood and plasma samples as was the protein-bound AA in an apolipoprotein B100 extract. Protein-bound phenylalanine enrichments were also measured in albumin and total protein from plasma plus samples from liver biopsies. The apolipoprotein B100 enrichments agreed well with those of the free AA in hepatic (and hepatic portal) plasma but were lower than for arterial free AA and greater than liver homogenate free AA. This adds support to the concept that export proteins may preferentially use AA directly from extracellular sources. Intake had no significant effect on constitutive liver protein synthesis and the values agreed well with those obtained by other isotopic approaches. There were, however, signicant declines, based on hepatic venous free phenylalanine enrichment, at the lower intake in both the fractional (3·4v. 4·7 % per d;P=0·024) and absolute (2·4v. 4·2 g/d;P=0·011) synthesis rates of albumin, which matched the estimated decrease in total plasma albumin content (52v. 67 g,P<0·01). In contrast, there was a smaller reduction in total plasma protein mass (145v. 151 g,P=0·035) with no observed significant difference in kinetic parameters. Albumin synthesis was calculated to account for a maximum of 17 % of total liver protein synthesis in the fed condition and this may fall to 8 % during moderate fasts.

scholarly journals Potential Analysis of Lemna sp. Extract as Immunostimulant to Increase Non-Specific Immune Response of Tilapia (Oreochromis niloticus) against Aeromonas hydrophila

2021 ◽  
Vol 8 (1) ◽  
pp. 40-47
Author(s):  
Eric Armando ◽  
Ayu Lestiyani ◽  
R. Adharyan Islamy
Keyword(s):  
Body Weight ◽  
Plasma Protein ◽  
Lysozyme Activity ◽  
Total Plasma ◽  
Immune Parameters ◽  
Randomized Design ◽  
Total Plasma Protein ◽  
Significant Difference ◽  
And Control ◽  
Range Test

Lemna sp. is known to have several bioactive compounds and polysaccharide macromolecules that can function as immunomodulators to affect non-specific immune responses to increase the body's resistance to pathogens. This study aims to determine the potential of catfish eye extract as an immunostimulant by observing non-specific tilapia immune parameters. The extraction method used was 96% ethanol maceration for 2 days with a ratio of 1: 4. The experimental design used a Completely Randomized Design with 5 treatments (doses 0.2, 0.4, 0.6 mg/kg, control + and control -) and 3 replications. The non-specific parameters of immunity observed included total plasma protein (Bradford method), superoxide dismutase and lysozyme activity. The data obtained will be analyzed using ANOVA, if there is a significant difference, it will be further tested with Duncan Multiple. Range Test (DMRT). The results showed that the highest total plasma protein was found in treatment C (giving an extract of 0.3 mg/kg body weight) with an average total plasma protein after 12 days of maintenance of 4.99 g / dL. The extract dose of 0.3 mg/body weight showed a rapid decrease in SOD and increase Lysozyme activity.

scholarly journals Effect of Acute Cardiac Tamponade on Red Cell Volume, Total Plasma Protein and Plasma Volume

1957 ◽  
Vol 5 (3) ◽  
pp. 257-260 ◽  
Author(s):  
REX J. UNDERWOOD ◽  
HERBERT E. GRISWOLD ◽  
WILLIAM A. HURST
Keyword(s):  
Cardiac Tamponade ◽  
Cell Volume ◽  
Plasma Protein ◽  
Plasma Volume ◽  
Red Cell ◽  
Total Plasma ◽  
Red Cell Volume ◽  
Total Plasma Protein
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