scholarly journals Bioactivity evaluation against Artemia salina Leach of medicinal plants used in Brazilian Northeastern folk medicine

2012 ◽  
Vol 72 (3) ◽  
pp. 505-509 ◽  
Author(s):  
DDR. Arcanjo ◽  
ACM. Albuquerque ◽  
B. Melo-Neto ◽  
LCLR. Santana ◽  
MGF Medeiros ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Moringa Oleifera ◽  
Folk Medicine ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Ageratum Conyzoides ◽  
Bioactive Natural Products ◽  
Flower Extract ◽  
The Mean ◽  
Ethanol Extracts

The brine shrimp (Artemia salina Leach) lethality bioassay offers an advantage in standardization and quality control of botanical products. This test is well correlated with antitumor activity (cytotoxicity) and can be used to monitor the activity of bioactive natural products. This paper reports the bioactivity of ethanol extracts from seven medicinal plants from the Northeast of Brazil (Acmella uliginosa, Ageratum conyzoides, Eugenia uniflora, Plectranthus neochilus, Moringa oleifera, Justicia pectoralis and Equisetum sp.) against Artemia salina. Biological activity was evaluated for extracts at 1, 10, 100, and 1000 µg/mL in triplicate, and the mean lethal concentration values (LC50) were obtained by probit analysis. The species Acmella uliginosa showed the highest bioactivity, and its flower extract was more active than its leaf extract.

scholarly journals ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2014 ◽  
Vol 14 (2) ◽  
pp. 106 ◽  
Author(s):  
Grace S. Baud ◽  
Meiske S. Sangi ◽  
Harry S.J. Koleangan
Keyword(s):  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Euphorbia Tirucalli ◽  
Ethanol Extracts

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.

scholarly journals Assessment of toxicity of Moringa oleifera flower extract to Biomphalaria glabrata, Schistosoma mansoni and Artemia salina

Chemosphere ◽  
2015 ◽  
Vol 132 ◽  
pp. 188-192 ◽  
Author(s):  
Cláudio A.A. Rocha-Filho ◽  
Lidiane P. Albuquerque ◽  
Luanna R.S. Silva ◽  
Patrícia C.B. Silva ◽  
Luana C.B.B. Coelho ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Moringa Oleifera ◽  
Biomphalaria Glabrata ◽  
Artemia Salina ◽  
Flower Extract

scholarly journals Yerba Mate (Ilex paraguariensis) Beverage: Nutraceutical Ingredient or Conveyor for the Intake of Medicinal Plants? Evidence from Paraguayan Folk Medicine

2018 ◽  
Vol 2018 ◽  
pp. 1-17 ◽  
Author(s):  
Monika Kujawska
Keyword(s):  
Medicinal Plants ◽  
Medicinal Plant ◽  
Moringa Oleifera ◽  
Folk Medicine ◽  
Ilex Paraguariensis ◽  
Yerba Mate ◽  
Importance Value ◽  
Important Species ◽  
Number Of Citations

The use of medicinal plants mixed with yerba mate (Ilex paraguariensis) has been poorly studied in the ethnopharmacological literature so far. The Paraguayan Mestizo people have the longest tradition of using the yerba mate beverage, apart from the indigenous Guarani people. This study analyses the role of yerba mate and medicinal plants in the treatment of illnesses within Paraguayan folk medicine. The research was conducted among 100 Paraguayan migrants living in Misiones, Argentina, in 2014 and 2015. Yerba mate is not considered to be a medicinal plant by its own virtues but is culturally a very important type of medicinal plant intake. Ninety-seven species are employed in hot and cold versions of the yerba mate beverage. The most important species are as follows: Allophylus edulis (highest number of citations), Aristolochia triangularis (highest relative importance value), and Achyrocline flaccida and Achyrocline tomentosa (highest score by Index of Agreement on Species). The plants are used in the treatment of 18 medicinal categories, which include illnesses traditionally treated with plants: digestive system, humoral medicine, and relatively new health conditions such as diabetes, hypertension, and high levels of cholesterol. Newly incorporated medicinal plants, such as Moringa oleifera, are ingested predominantly or exclusively with the mate beverage.

scholarly journals Phenolic compounds, flavonoids and antioxidant activity of leaves, flowers and roots of goat weed

2014 ◽  
Vol 32 (4) ◽  
pp. 486-490 ◽  
Author(s):  
Rosana GR Dores ◽  
Sarah F Guimarães ◽  
Tatiane V Braga ◽  
Maira CM Fonseca ◽  
Pauliane M Martins ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Functional Food ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Positive Control ◽  
Cellular Aging ◽  
Ageratum Conyzoides ◽  
Ethanol Extracts ◽  
Bioanalytical Validation

Ageratum conyzoides (mentrasto or goat weed) has invaluable socio-economic-cultural importante being used as unconventional vegetable in traditional cuisine of Minas Gerais state, Brazil. In folk medicine it is used as a purgative, febrifuge, antiemetic and against colic, ulcers and uterine problems. The objective of this study was to quantify the concentration of phenolic compounds, flavonoids and antioxidant activity in ethanol extracts of goat weed, as a step towards analytical and bioanalytical validation of its use as aherbal and/or medication as functional food. Thus, analysis of the levels of phenolic, flavonoids and antioxidant activity (AAT %) were made in ethanol extracts of leaves (EF), flowers (EFL) and roots (ER) of goat weed. Measuring phenolic compounds in EF, ER and EFL, was carried out by the Folin-Ciocalteu method. The content of flavonoids in the extracts was determined using vanillin in acid medium. For the antioxidant evaluation we used the methodology of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging method. The experimental design was randomized blocks design, all analyzes being performed with four replications. Data were submitted to analysis of variance and to test at 1% significance. ER had a higher concentration of phenolic compound (23.33 mg/mL), followed by EFL (19.254 mg/mL) and PE (14.722 mg/mL). The highest content of flavonoids was EF (5.726 µg/mL), followed by EFL (5.463 µg/mL) and ER (4.805 µg/mL). The antioxidant activity of EFL (87.62%) was similar to the positive control validating the potential of this species as a functional food and the development of standardized herbal formulation that can be used for preventing of degenerative diseases and cellular aging.

Artemia species: An Important Tool to Screen General Toxicity Samples

2020 ◽  
Vol 26 (24) ◽  
pp. 2892-2908 ◽  
Author(s):  
Epole Ntungwe N ◽  
Eva M. Domínguez-Martín ◽  
Amilcar Roberto ◽  
Joana Tavares ◽  
Vera M. S. Isca ◽  
...  
Keyword(s):  
Natural Products ◽  
Medicinal Plants ◽  
Brine Shrimp ◽  
Low Cost ◽  
Artemia Salina ◽  
Toxicity Assay ◽  
Brine Shrimp Lethality ◽  
Bioactive Natural Products ◽  
Therapeutic Drugs ◽  
Brine Shrimp Lethality Assay

Medicinal plants are a good source of novel therapeutic drugs, due to the phytochemicals present. Artemia, commonly known as brine shrimp, is a tiny halophilic invertebrate belonging to class Crustacean, which plays an important role in saline aquatic and marine eco-systems. Besides its usage in aquaculture, it is also highly valued for its application in toxicity detection and it is used in areas such as Ecology, Physiology, Ecotoxicology, Aquaculture and Genetics. Furthermore, Artemia based lethality assay (brine shrimp lethality assay, BSLA) is rapid, convenient and low cost. Presently, brine shrimp lethality assays are enormously employed in research and applied toxicology. It has been used in the study of natural products as a preliminary toxicity assay to screen a large number of extracts and compounds for drug discovery in medicinal plants. The aim of this review paper is to collect, organize, select and discuss the existing knowledge about the different uses of Artemia salina as a bench-top bioassay for the discovery and purification of bioactive natural products.

scholarly journals Cytotoxic Effect of Five Medicinal Plants Extracts Using Brine Shrimp (Artemia salina) TEST

2012 ◽  
Vol 47 (1) ◽  
pp. 133-136
Author(s):  
R Akter ◽  
Mohammed A Satter ◽  
M S Khan ◽  
M S Rahman ◽  
N U Ahmed
Keyword(s):  
Medicinal Plants ◽  
Brine Shrimp ◽  
Cytotoxic Effect ◽  
Curcuma Longa ◽  
Artemia Salina ◽  
Curcuma Zedoaria ◽  
Scoparia Dulcis ◽  
Potential Sources ◽  
Ethanol Extracts ◽  
Streblus Asper

Medicinal plants like Curcuma longa, Curcuma zedoaria, Streblus asper, Enhydra fluctunas and Scoparia dulcis are commonly available in   Bangladesh. This study investigated the effect of ethanol extracts from these five medicinal plants on the viability of Brine Shrimp larvae.   The lethal concentrations (LC50 values) of the extracts were determined. LC50 values of C. longa, C. zedoaria and S. asper were less than   250?g/ml and that of E. fluctunas was greater than 250 ?g/ml. Among five plants tested, one (S. dulcis) appear to be inactive. Results indicate   that C. longa, C. zedoaria and S. asper could be potential sources of bio-active compounds DOI: http://dx.doi.org/10.3329/bjsir.v47i1.10724 Bangladesh J. Sci. Ind. Res. 47(1), 133-136, 2012

Protective Effect of Moringa Oleifera Leaves Against TramadolInduced Nephrotoxicity in Mice

2017 ◽  
Vol 9 (02) ◽  
Author(s):  
Ashraf Albrakati
Keyword(s):  
Oral Dose ◽  
Moringa Oleifera ◽  
Treated Group ◽  
Control Group ◽  
Serum Urea ◽  
Kidney Function Tests ◽  
Mean Values ◽  
Intraperitoneal Dose ◽  
Moringa Oleifera Leaves ◽  
The Mean

Tramadol, a broadly in recent years, is an effective analgesic agent for the treatment of moderate to acute pain. Its metabolites are excreted by the kidney which may cause nephrotoxicity. Moringa oleifera leaves are commonly used to provide herbal and plant-derived medicinal products especially in developing nations. The present study was carried out to determine the biochemical and histopathological changes in the kidney of tramadol-treated albino mice and to evaluate the possible protective role of Moringa oleifera leaves against tramadol-induced nephrotoxicity. Twenty adult albino mice were divided into four groups. Control group (group i) received daily intraperitoneal injection of normal saline only, group ii received oral dose of Moringa oleifera leaves extract (20 mg/kg/bw) for three weeks, group iii received daily intraperitoneal dose of tramadol (0.3 mg/kg/bw) for the same period, group iv, received daily oral dose of Moringa oleifera leaves extract, (20 mg/kg/bw) three hours before injecting intraperitoneal dose of tramadol (0.3 mg/kg/bw), for the same period. Blood samples were withdrawn at the end of the experiment for kidney function tests and specimens from the kidney were processed for histological study. No significant differences in the mean values of the kidney function tests were noticed between Moringa oleifera group and control group. However, there was highly significant increase in the mean values of serum, urea and creatinine in tramadol-treated group as compared to the control group. Although tramadol + Moringa oleifera group revealed significant difference in the mean values of urea and creatinine when compared with tramadol-treated group. So, Moringa oleifera leaves extract have been shown to attenuate the renal dysfunction, improve the renal architecture, with nearly normalization of serum urea and creatinine levels which indicate improvement of renal function. In conclusion, in the light of biochemical results and histological findings, co-administration of Moringa oleifera leaves lessened the negative effects of tramadol-induced nephrotoxicity; possibly by its antioxidant action. Further investigation of these promising protective effects of Moringa oleifera leaves against tramadol-induced renal injury may have considerable impact on developing an adjunct therapy aiming to improve the therapeutic index of some nephrotoxic drugs.

Bis (Isothiocyanatomethyl) Benzene, A Plant Derived Anti-Neoplastic Compound: Purified from Moringa Oleifera Leaf Extract

2019 ◽  
Vol 19 (5) ◽  
pp. 677-686 ◽  
Author(s):  
Samrat Paul ◽  
Piyali Basak ◽  
Namrata Maity ◽  
Chayan Guha ◽  
Nandan Kumar Jana
Keyword(s):  
Anticancer Activity ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Folk Medicine ◽  
Drug Formulation ◽  
Indigenous Species ◽  
Cancer Cell Growth ◽  
Apoptotic Pathway ◽  
Dimeric Form ◽  
Mcf 7

Background: Moringa oleifera lam, commonly known as “Sajina”, is an indigenous species to India. In our folk medicine, it is used for the treatment of Canker (cancer). The Moringa oleifera leaf extract contains many phyto-compounds, with some being anti-neoplastic in nature. Objective: Our preliminary study showed that the leaf extract significantly kills cancer cells compared to normal cells. On searching for the new phyto-compound, Bis-isothiocyanatomethyl) benzene was purified and isolated. Methods: The sequential process of fractional distillation, column chromatography, followed by TLC and HPLC is performed for purification. Every fraction from each step was tested on HeLa cell line for evaluating the presence of the phyto-compound. Results and Conclusion: FTIR peak analysis of a single phyto-compound shows the presence of thiocyanate group, aromatic carbon group. 1H & 13C NMR peak analysis along with High-resolution mass spectroscopy (HRMS) calculation confirm the chemical structure with IUPAC name [Bis (Isothiocyanatomethyl) benzene]. Previously, Isothiocyanatomethyl- benzene solely or in conjugation with sugar molecule has been reported, but its dimeric form in nature hasnot yet been published anywhere. It shows anticancer activity by retarding cancer cell growth & inhibits carcinogenesis on HeLa, MCF-7, and MDA-MB-231 cell lines by caspase 3 apoptotic pathway and showed comparatively less cytotoxicity to PBMC cell. It shows anticancer activity almost the same as the market available drug Cis-Platin. Therefore, further extrapolating its activity with different concentrations may result in its use as a drug formulation for the treatment of cancer.

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