Ruminal parameters and ruminal degradability of feedlot sheep fed safflower grains

SUMMARY This study aimed to evaluate the rumen degradability of dry matter (DM), disappearance of neutral detergent fiber (NDF) of Tifton 85 hay, and fermentation parameters (pH and ruminal ammonia nitrogen) of sheep fed diets with inclusion of safflower grains (0, 7.50 and 15%). Three mixed breed lambs with average body weight of 35.50± 1.50 kg were used. The rumen degradability of dry matter and NDF of the Tifton 85 haywas analyzedby the disappearance of DM and NDF, and the kinetic parameters adjusted according to the first order asymptotic model. The samples were incubated in the rumen in descending order (96, 48, 24, 12, 6, 3, 0 hours). The determination of ruminal pH and ruminal ammonia nitrogen (RAN) was performed before feeding and 2, 4, 6 and 8 hours after feeding. Data of ruminal fermentation were tested by analysis of variance with repeated measures in time, at 5% probability. Ruminal pH values were not statistically different. When evaluating the RAN, there was a significant difference between the times and treatments for the diet with 15% safflower. The diets presented low effective degradability of DM and NDF with mean values of 18.02% and 47.40%, respectively. The safflower grain can be used up to 7.50% in the diets without altering the degradability and ruminal fermentation.

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Use of babassu cake and pineapple byproduct silage in the diets of feedlot sheep

Semina Ciências Agrárias ◽

10.5433/1679-0359.2021v42n2p795 ◽

2021 ◽

Vol 42 (2) ◽

pp. 795-808

Author(s):

Ernestina Ribeiro Santos Neta ◽

◽

Daiany Iris Gomes ◽

Luis Rennan Sampaio Oliveira ◽

Rafael Mezzomo ◽

...

Keyword(s):

Soybean Meal ◽

Crude Protein ◽

Dry Matter ◽

Ammonia Nitrogen ◽

Latin Squares ◽

Neutral Detergent Fiber ◽

Elephant Grass ◽

Ruminal Ph ◽

Grass Silage ◽

Babassu Cake

This study evaluated the use of babassu cake as a replacement for soybean meal and pineapple byproduct silage as roughage in the diets of feedlot sheep. Ten rumen-fistulated sheep were used and distributed in an experimental design consisting of two 5 x 5 Latin squares. Babassu cake replaced soybean meal at four concentration levels (0, 26.66, 53.33, and 80%), and pineapple byproduct silage was used as roughage. A treatment was also evaluated in which elephant grass silage was used as roughage and corn and soybean meal were used as the concentrate. No effect of babassu cake as a replacement for soybean meal on dry matter intake, neutral detergent fiber, or crude protein was observed. Higher babassu cake replacement levels in the diets resulted in linear reductions in the digestibility of the dry matter, neutral detergent fiber, organic matter, nonfiber carbohydrates and total carbohydrates. For the digestibility of the crude protein, no effect was observed after the addition of babassu cake. The replacement levels of the babassu cake had no effect on the intake and retention of the daily nitrogen, ammonia nitrogen and ruminal pH. Babassu cake could replace soybean meal at concentrations of up to 80% when the roughage source was pineapple byproduct silage. Compared with elephant grass silage, pineapple byproduct silage improves nutrient intake and digestibility and nitrogen intake, retention, and absorption without compromising the ruminal pH or ammonia nitrogen of feedlot sheep.

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Citrus pulp in lamb diets: intake, digestibility, and ruminal parameters

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n5p3421 ◽

2015 ◽

Vol 36 (5) ◽

pp. 3421 ◽

Cited By ~ 2

Author(s):

Eduardo Lucas Terra Peixoto ◽

Mirton José Frota Morenz ◽

Carlos Elysio Moreira da Fonseca ◽

Elizabeth Dos Santos Moura ◽

Karla Rodrigues de Lima ◽

...

Keyword(s):

Dry Matter ◽

Rumen Fluid ◽

Latin Square ◽

Neutral Detergent Fiber ◽

Average Weight ◽

Corn Meal ◽

Ruminal Fermentation ◽

Ruminal Ph ◽

Citrus Pulp ◽

Fermentation Parameters

<p>This study aimed to evaluate the viability of replacing corn meal with citrus pulp (0, 25, 50, 75, and 100 % dry matter of corn meal) by evaluating several nutritional parameters such as intake and digestibility of nutrients, and ruminal fermentation parameters. The diets were formulated to be isoproteic with a roughage:concentrate ratio of 60:40. Five crossbred lambs with an initial average weight of 26.1 ± 1.8 kg were used and distributed in a 5 x 5 Latin Square design. For digestibility of nutrients was carried out to feed, orts, and feces collection. The evaluated nutrients were dry matter, crude protein, ether extract, ash, neutral detergent fiber, fiber acid detergent and lignin. Were determined nitrogen and carbohydrate fractions, and ruminal fermentation parameters (N-NH 3 and ruminal pH). The results were subjected to analysis of variance and regression analysis (t-test; ? = 0.05). Citrus pulp inclusion in the diets did not affect intake and digestibility of nutrients, or the pH and the NH3-N content of the rumen fluid. Citrus pulp can be used as a total substitute for corn in concentrate or up to 26.5% in the total ration for lambs (dry basis). </p>

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Soybean hulls and / or white oat grains on the ingestive behavior of confined steers

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n5p1925 ◽

2019 ◽

Vol 40 (5) ◽

pp. 1925

Author(s):

Guilherme Joner ◽

Dari Celestino Alves Filho ◽

Andrei Retamoso Mayer ◽

Patrícia Machado Martini Cattelam ◽

Camille Carijo Domingues ◽

...

Keyword(s):

Repeated Measures ◽

Dry Matter ◽

Block Design ◽

Neutral Detergent Fiber ◽

Ingestive Behavior ◽

Raw Material ◽

Feeding Time ◽

Soybean Hulls ◽

Significance Level ◽

Significant Difference

Dry matter and neutral detergent fiber contents act as physical regulators of voluntary intake in ruminants. Therefore, different levels of these components may interfere with animal performance and require careful assessment of the used raw material. This study was conducted with the purpose to assess the effect of soybean hulls and white oat in distinct or associated uses in the concentrate diet on the ingestive behavior of cattle. Thirty-six steers with an initial mean age of 20 months and initial mean weight of 226 kg were used. The diet was composed of 50% sorghum silage and 50% concentrate based on dry matter. The tested diets were soybean hulls (concentrate fraction composed mainly of soybean hulls), white oat grains (concentrate fraction composed mainly of white oat grains), and mixture (concentrate fraction composed of equal parts of soybean hulls and white oat grains). The experimental design was a randomized block design with 12 animals per diet. The data were compared by the PROC MIXED procedure with repeated measures in time, and the means were compared by the least significant difference test at 5% significance level. Diets presented different contents of neutral detergent fiber (66, 56, and 47%) and lignin (3.2, 3.7, and 4.1%) as the percentage of white oat grain increased in the diet. The total rumination time was lower (447 minutes) for animals that received equal parts of soybean hulls and white oat grains in the diet when compared to animals receiving a diet containing soybean hulls (483 minutes) and white oat grains (495 minutes), with no difference from each other. Animals fed a diet containing soybean hulls and white oat grains showed longer feeding time, a higher number of daily meals, shorter rumination time, shorter time spent chewing, and fewer number of chews per bolus and day. This diet results in a better dry matter and neutral detergent fiber rumination efficiencies.

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PSXIII-26 Supplementation with a polyphenolic pine bark extract reduces ammonia nitrogen production in a RUSITEC system as determined by principal component analysis

Journal of Animal Science ◽

10.1093/jas/skab235.779 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 435-436

Author(s):

Nelson Vera ◽

Constanza Gutierrez ◽

Pamela Williams ◽

Cecilia Fuentealba ◽

Rodrigo Allende ◽

...

Keyword(s):

Principal Component Analysis ◽

Repeated Measures ◽

Pearson Correlation ◽

Principal Component ◽

Component Analysis ◽

Ammonia Nitrogen ◽

Pine Bark ◽

Bark Extract ◽

Ruminal Fermentation ◽

Fermentation Parameters

Abstract The aim of the study was to correlate the effects of supplementation with a polyphenolic pine bark extract (PBE) in diets with different forage to concentrate (F:C) ratio on methane (CH4), ammonia nitrogen (NH3–N) production and ruminal fermentation parameters using the Rumen Simulation Technique (RUSITEC). The experimental diets were F:C 70:30 (HF) or F:C 30:70 (HC) with or without 2% PBE on a DM basis. The four diets were isoproteic (15% CP), with similar OM (HF 94% and HC 96%), but different NDF (HF 40% and HC 25%). The treatments, in duplicate, were assigned in an 8 fermenter RUSITEC apparatus. Incubations were run twice, with 5 days of sampling after 10 days adaptation. The experimental design was a 2x2 factorial arrangement in a randomized complete block with repeated measures. Pearson correlation and principal component analysis (PCA) were conducted to elucidate relationships among PBE total polyphenols (TP) and the variables evaluated. The TP was highly correlated with NH3–N (r = –0.98; P < 0.001) and butyrate (r = –0.85; P < 0.001), and had a high correlation with propionate (r = 0.75; P < 0.001) and acetate (r = 0.68; P = 0.001). Correlation with total VFA was moderate (r = –0.59; P = 0.006), and CH4 yield and IVDMD there were not correlated (r ≤ –0.07; P ≥ 0.188). The PCA (KMO = 0.655; BTS < 0.001) shows that 75.2% of the total variation is explained by the first two principal components (PC1 = 46.5% and PC2 = 28.7%). In the score plot, PC1 discriminated between diets with and without PBE, while the PC2 separated based on NDF. The loading plot showed that TP and propionate were clustered, and had inverse directions to NH3–N. In conclusion, the PBE supplementation reduces NH3–N production in a RUSITEC system without decreasing CH4 yield or negatively affecting ruminal fermentation parameters.

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PSX-B-10 Effect of undigested neutral detergent fiber concentration and forage inclusion rate on ruminal pH, reticular motility, and total tract permeability for finishing beef heifers

Journal of Animal Science ◽

10.1093/jas/skab235.811 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 457-458

Author(s):

Murillo C Pereira ◽

Karen A Beauchemin ◽

Tim A McAllister ◽

Wenzhu Yang ◽

Joyce Van Donkersgoed ◽

...

Keyword(s):

Gastrointestinal Tract ◽

Dry Matter ◽

Latin Square ◽

Barley Grain ◽

Neutral Detergent Fiber ◽

Fiber Concentration ◽

Eating Time ◽

Ruminal Ph ◽

Contraction Duration ◽

Gastrointestinal Permeability

Abstract This study evaluated the effects of undigested neutral detergent fiber (uNDF) concentration and forage inclusion (FI) rate on dry matter (DM) intake, ruminal pH, reticular contractions, and gastrointestinal permeability for finishing beef cattle. Five ruminally cannulated Hereford′Simmental heifers (699±69.1 kg) were used in an incomplete 6×6 Latin square (26-d periods) with a 2×3 factorial treatment arrangement. Barley grain-based diets were formulated using barley silage or wheat straw to provide low or high uNDF (7.1 vs. 8.5% DM) with forage proportions of 5, 10, or 15% of dietary DM. Dry matter intake (P ≥ 0.10) and eating time (P ≥ 0.13) were not affected by uNDF, FI, or uNDF′FI. With low uNDF diets, increasing FI numerically (P = 0.02) increased rumination time (min/d); while, with high uNDF diets, rumination time increased with 5 to 10% FI, but not thereafter (P = 0.03). Mean ruminal pH was not affected by uNDF (6.17 vs. 6.19; P = 0.08), but increased with increasing FI (6.04b, 6.23a, and 6.28a; P = 0.02). Duration of ruminal pH < 5.5 was not affected by uNDF but tended (P = 0.07) to be reduced with increasing FI. High uNDF diet tended to increase the frequency of reticular contractions (1.43 vs. 1.51 contractions/min; P = 0.07) but decreased the contraction duration (13.2 vs. 14.1 sec; P = 0.04). Increasing FI increased contraction frequency (1.39b, 1.50a, and 1.53a contractions/min; P = 0.03) and tended to reduce contraction duration as forage increased from 5 to 10 and 15% (14.3, 13.1, and 13.6 sec; P = 0.07). Feeding high uNDF decreased (P = 0.05) permeability of the gastrointestinal tract based on the appearance of Cr-EDTA in urine following an intra-ruminal dose. Increasing FI tended to reduce gastrointestinal tract permeability (P = 0.06). Limited interactions indicate that uNDF and FI act independently suggesting that increasing dietary uNDF, without increasing FI rate, can stimulate frequency of reticulo-ruminal contractions and reduce gastrointestinal permeability for finishing cattle.

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Dietary rambutan peel powder as a rumen modifier in beef cattle

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.19.0342 ◽

2020 ◽

Vol 33 (5) ◽

pp. 763-769

Author(s):

Thiwakorn Ampapon ◽

Metha Wanapat

Keyword(s):

Beef Cattle ◽

Dry Matter ◽

Volatile Fatty Acids ◽

Latin Square ◽

Neutral Detergent Fiber ◽

Napier Grass ◽

Feed Consumption ◽

Ruminal Fermentation ◽

Fruit Peel ◽

Negative Effect

Objective: The experiment was conducted to study the effect of rambutan (Nephelium lappaceum) fruit peel powder (RP) on feed consumption, digestibility of nutrients, ruminal fermentation dynamics and microbial population in Thai breed cattle.Methods: Four, 2-year old (250±15 kg) beef bull crossbreds (75% Brahman×25% local breed) were allotted to experimental treatments using a 4×4 Latin square design. Four dietary supplementation treatments were imposed; non-supplementation (control, T1); supplementation of RP fed at 2% of dry matter intake (DMI) (low, T2); supplementation of RP fed at 4% of DMI (medium, T3) and supplementation of RP fed at 6% of DMI (high, T4). All cattle were given a concentrate supplement at 1% of body weight while Napier grass was provided as a free choice.Results: The findings revealed that RP supplementation did not negatively affect (p>0.05) DMI of Napier grass, while RP intake and total DMI were the greatest in the RP supplementation at 4% and 6% DMI. Nevertheless, the nutrients (dry matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber) digestibilities were not changed in the RP supplementation groups. Rumen fermentation parameters especially those of total volatile fatty acids, acetate and butyrate were not significantly changed. However, the propionate concentration was remarkably increased (p<0.05) in the RP supplementation. Notably, the ratio of acetate to propionate, the number of protozoa, as well as the methane estimation were significantly reduced in the RP supplemented groups (4% and 6% of DMI), while the counts of bacteria was not altered.Conclusion: Supplementation of RP (4% of DMI) improved rumen propionate production, reduced protozoal population and methane estimation (p<0.05) without a negative effect on feed consumption and nutrients total tract digestibilities in beef cattle. Using dietary rambutan fruit peel powder has potential promise as a rumen regulator.

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Nutritional Quality and In Vitro Rumen Fermentation Characteristics of Silage Prepared with Lucerne, Sweet Maize Stalk, and Their Mixtures

Agriculture ◽

10.3390/agriculture11121205 ◽

2021 ◽

Vol 11 (12) ◽

pp. 1205

Author(s):

Musen Wang ◽

Fujin Zhang ◽

Xinxin Zhang ◽

Ying Yun ◽

Lei Wang ◽

...

Keyword(s):

Organic Matter ◽

Nutritional Quality ◽

Dry Matter ◽

Rumen Fermentation ◽

Gas Production ◽

Neutral Detergent Fiber ◽

Ruminal Fermentation ◽

Fresh Matter ◽

In Vitro Rumen Fermentation

The objective of this work was to evaluate the pH, chemical composition, minerals, vitamins, and in vitro rumen fermentation characteristics of silage prepared with lucerne, sweet maize stalk (MS), and their mixtures. Freshly chopped lucerne and MS were combined in ratios of 100:0 (M0, control), 80:20 (M20), 60:40 (M40), 40:60 (M60), 20:80 (M80), and 0:100 (M100) on a fresh matter basis. Each treatment was prepared in triplicate, and a total of eighteen silos were fermented for 65 days. After 65 days of fermentation, the pH values in M0, M20, M40, M60, M80, and M100 silages were 5.47, 4.84, 4.23, 4.13, 3.79, and 3.61, respectively. As the MS proportion in the mixtures increased, silage K, Ca, P, Na, Fe, and Cu concentrations linearly decreased (p < 0.001) and so did vitamins B5 and K1 and α-tocopherol. In vitro rumen dry matter and organic matter degradability, pH, ammonia, total volatile fatty acid, and gas production linearly decreased (p < 0.01), while neutral detergent fiber concentration linearly increased (p < 0.001), with increasing proportion of MS. The in vitro dry matter and organic matter degradability rapidly decreased when the MS percentage was ≥60%. In conclusion, the M40 silage is the most suitable for livestock utilization in local forage production considering the balance of silage pH, nutritional quality, and in vitro ruminal fermentation characteristics.

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Effects of monensin inclusion and level of intake in limit-feeding strategies for beef cows1

Translational Animal Science ◽

10.1093/tas/txaa108 ◽

2020 ◽

Vol 4 (3) ◽

Author(s):

Caleb J Boardman ◽

Tryon A Wickersham ◽

Levi A Trubenbach ◽

Jason E Sawyer

Keyword(s):

Dry Matter ◽

Feeding Strategies ◽

Ruminal Fermentation ◽

Acid Detergent Fiber ◽

Passage Rate ◽

Ruminal Ph ◽

Energy Retention ◽

Gross Energy ◽

Predicted Values ◽

Limit Feeding

Abstract A study was conducted to evaluate the effects of intake management and ionophore inclusion on diet utilization under managed intake conditions in beef cattle. Two experiments utilized common diets fed at 120% (H) or 80% (L) of maintenance with either 0 or 200 mg/d monensin in a factorial arrangement. Forty cows were fed for 56 d (Exp. 1) to evaluate effects on diet utilization and energy retention; diets were fed to 16 ruminally cannulated steers (Exp. 2) to determine effects on digestion, energy value, and ruminal fermentation. Cows fed H had greater body weight (BW) gain (P < 0.01) and retained energy (RE; P < 0.01), although estimated heat production was also greater (P < 0.01). Monensin had limited effects on overall BW gain (P = 0.97). Monensin had no effect on RE (P = 0.94) or calculated heat energy (HE; P = 0.53) despite effects on diet utilization observed in steers. In steers, L increased (P < 0.01) digestion of dry matter, organic matter, acid detergent fiber, and gross energy (GE) and reduced (P < 0.01) passage rate; monensin did not affect digestion (P > 0.15) but did reduce passage rate (P < 0.03). Monensin lowered (P < 0.01) acetate:propionate ratio and increased (P < 0.05) ruminal pH. Monensin did not alter feed required for maintenance; however, limit-feeding reduced apparent daily maintenance requirement to 62.85 kcal/kg BW0.75, a 26% reduction from model-predicted values.

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Effect of stage of maturity at harvest for forage pea (Pisum sativum L.) on eating behavior, ruminal fermentation, and digestibility when fed as hay to yearling beef heifers

Translational Animal Science ◽

10.1093/tas/txz167 ◽

2019 ◽

Vol 4 (1) ◽

pp. 149-158 ◽

Cited By ~ 1

Author(s):

Alex A Pursley ◽

Bill Biligetu ◽

Tom Warkentin ◽

Herbert A Lardner ◽

Gregory B Penner

Keyword(s):

Pisum Sativum ◽

Eating Behavior ◽

Latin Square ◽

Neutral Detergent Fiber ◽

Starch Digestibility ◽

Ruminal Fermentation ◽

Beef Heifers ◽

Passage Rate ◽

Ruminal Ph ◽

Pisum Sativum L

Abstract The objective of this study was to evaluate the stage of maturity at harvest for pea hay (Pisum sativum L., c.v. CDC Horizon) on dry matter intake (DMI), eating behavior, ruminal fermentation, and digestibility when fed to beef heifers. Pea hay was cut at EARLY (defined to occur when flat pods were on one or more nodes), MID (when seeds filled the pods at one or more nodes and the leaves were changing from green to gold), and LATE (yellow dry seeds filled pods on most or all of the nodes and the pods and leaves had a yellow color) phases, and was cured in the field and baled. Six ruminally-cannulated Speckle Park heifers were used in a replicated 3 × 3 Latin square design with three 18-d periods including 12 d for adaptation, 2 d for measurement of ruminal pool sizes, and 4 d for the collection of eating behavior, ruminal pH, ruminal digesta, and feces. For all treatments, the respective pea hay was included at 40% of the dietary DM. Stage of maturity at harvest for pea hay did not affect total DMI, pea hay DMI, or the total short-chain fatty acid concentration in ruminal fluid with averages of 8.6 kg/d, 3.2 kg/d, and 96.55 mM, respectively. The duration of time spent ruminating decreased with advancing pea hay maturity when reported as min/d, min/kg DMI, and min/kg neutral detergent fiber (NDF) (P ≤ 0.01). Mean ruminal pH also decreased with advancing pea maturity (P < 0.01). The ruminal DM and undigested NDF corrected for OM pools were not affected by stage of maturity (P ≥ 0.55) nor was the rate of digestion for NDF. However, NDF passage rate decreased by 0.21%/h with advancing pea hay maturity (P = 0.02). Apparent total tract digestibility of NDF (average = 16.30%, P = 0.41) was not affected, but starch digestibility decreased from 96.10% to 93.08% with advancing pea hay maturity (P = 0.07). Overall, stage of maturity at harvest for pea hay does not appear to affect DMI or NDF digestibililty but decreases chewing activity, apparent total tract starch digestibility, ruminal pH, and ruminal NDF passage rate.

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PSIV-22 The effects of altering ruminal fermentable carbohydrate supply in finishing diets containing distillers grains on rumen fermentation characteristics and nitrogen utilization

Journal of Animal Science ◽

10.1093/jas/skz258.450 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 220-220

Author(s):

Cody Ream ◽

Allison V Stevens ◽

Gwinyai Chibisa

Keyword(s):

Dry Matter ◽

Latin Square ◽

Ammonia Concentration ◽

Distillers Grains ◽

Ruminal Fermentation ◽

Carbohydrate Source ◽

Finishing Cattle ◽

Finishing Diets ◽

Ruminal Ph ◽

Total Urine

Abstract This study examined the effects of altering ruminal fermentable carbohydrate (RFC) supply by feeding different amounts of corn and wheat in finishing cattle diets containing 15% corn dried distillers grains [DDGS; dry matter (DM) basis] on ruminal fermentation characteristics and nitrogen (N) utilization. Six ruminally-cannulated crossbred heifers were used in a replicated 3 × 3 Latin square design with 28 d periods. Dietary treatments were either corn (73% of diet DM; CON), 53:20 corn:wheat blend (20W) or 33:40 corn:wheat blend (40W) as the major fermentable carbohydrate source. Diets were isonitrogenous (12% CP; DM basis). Feed intake was measured daily. Indwelling pH logger were used to measure ruminal pH (d 22 to 28) and ruminal fluid was collected from d 26 to 28 to determine fermentation characteristics, as were feces and urine to measure N excretion. Data was analyzed using PROC MIXED in SAS. Dry matter and N intake tended to be lower (P ≤ 0.07) for heifers fed the 40W compared to the CON and 20W diets. There was no diet effect (P = 0.15) on total VFA concentration; however, the duration and area for pH < 5.5 tended to be longer (P = 0.07) and greater (P = 0.096), respectively, for heifers fed the 20W and 40W compared to the CON diet. There was no diet effect (P ≥ 0.44) on ruminal ammonia concentration and total urine N excretion (g and % of N intake). However, urine urea N (UUN) excretion as a percentage of total urine N tended to be lower (P = 0.05) in heifers fed the 20W and 40W compared to the CON diet. In summary, although altering RFC supply by feeding increasing amounts of wheat resulted in a decrease in UUN excretion, it also caused a decrease in ruminal pH that possibly compromised DM intake.

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