scholarly journals DNA index and anatomical aspects of the micrografting of dragon fruit on different rootstocks

2021 ◽  
Vol 56 ◽  
Author(s):  
Renata Amato Moreira ◽  
Mariane Aparecida Rodrigues ◽  
Deniete Soares Magalhães ◽  
Leila Aparecida Salles Pio ◽  
Dalilhia Nazaré dos Santos ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Experimental Design ◽  
Dna Content ◽  
Dna Index ◽  
Dragon Fruit ◽  
Before And After ◽  
Hylocereus Undatus ◽  
Hylocereus Polyrhizus ◽  
Made In

Abstract The objective of this work was to evaluate the viability of the micrografting of yellow dragon fruit (Selenicereus megalanthus) on different rootstocks, based on DNA content and anatomical analyses. The used rootstocks were: yellow dragon fruit, white dragon fruit (Hylocereus undatus), Saborosa (Selenicereus setaceus) dragon fruit, and the Cebra and Orejona red dragon fruit (Hylocereus polyrhizus) varieties. The experimental design was completely randomized with five treatments and four replicates of five plants. After 30 days of cultivation, the following traits were evaluated: length and diameter of the micrografts and microrootstocks; and root length, percentage of setting, and fresh mass of the micrografts. Flow cytometry analyzes were performed before and after micrografting to verify genetic stability and the occurrence of endoreduplication. In addition, histological sections were made in the micrografting region to verify the connections of vessels and tissues between the graft and the rootstock. Endoreduplication was observed in all treatments. The amount of DNA in the yellow dragon fruit micrograft increased on the red Orejona variety. The presence of vessel connections was verified between the micrografts and microrootstocks. The yellow dragon fruit was also more vigorous when grafted on Orejona. Based on DNA content and anatomical analyses, in vitro yellow dragon fruit micrografting is feasible in all used rootstocks.

scholarly journals Pemanfaatan Jamur Trichoderma sp Sebagai Antagonis Patogen Busuk Sulur Tanaman Buah Naga Merah (Hylocereus polyrhizus) Secara In Vitro

2018 ◽  
Vol 7 (1) ◽  
pp. 19
Author(s):  
Herlyan Prasetiyo ◽  
Purwati Purwati ◽  
Iin Arsensi
Keyword(s):  
Plant Disease ◽  
Secondary Data ◽  
Pathogenic Fungi ◽  
Primary Data ◽  
Field Observations ◽  
Trichoderma Sp ◽  
Dragon Fruit ◽  
Fruit Plants ◽  
Hylocereus Polyrhizus

Utilization of Trichoderma sp fungi as pathogenic fungi antagonists in red dragon fruit plants (Hylocereus polyrhizus) in vitro. The purpose of this study was to identify foul pathogens of dragon fruit plants and then test the ability of Trichoderma sp antagonists to deciduous pathogens of red dragon fruit plants in vitro. The study was conducted from August to October 2016, The research was conducted at the Laboratory of Pest and Plant Disease Sciences, Faculty of Agriculture, Mulawarman University, Samarinda. Sampling of plants exposed to foul tendrils was carried out in Bukit Merdeka Village, Samboja District, Kutai Kartanegara Regency. There are two data observed in this study, primary data and secondary data. Primary data is data obtained directly from the source through direct field observations and laboratory observations and secondary data data obtained from interviews with farmers. The results showed that the pathogen that causes tendon rot in dragon fruit plants is the fungus Colletotrichum gloesporioides (penz. Ssaac). Trichoderma sp can inhibit the development of pathogens Colletotrichum gloesporioides (Penz.) Ssaac., With the highest average resistance of 71.85%. 

scholarly journals CHARACTERISTICS ANALYSIS OF DRAGON FRUIT EXTRACT SKIN BETACYANIN of Hylocereus polyrhizus AND Hylocereus undatus WITH TEST OF STABILITY ORGANOLEPTIC JELLY AS ATLAS MEDIA

2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Tutut Puji Lestari
Keyword(s):  
High School ◽  
Phase I ◽  
Junior High School ◽  
Junior High ◽  
Phase Iii ◽  
Fruit Skin ◽  
Dragon Fruit ◽  
Hylocereus Undatus ◽  
Hylocereus Polyrhizus ◽  
Phase I And Ii

Dragon fruit Hylocereus polyrhizus  and Hylocereus undatus are familiy of cactus, grown in Malang. The high consumption of dragon fruit, have an impact on the fruit skin buildup that simply disposed of as trash. Dragon fruit skin is known to have a source of natural red dye, which is Betacyanin. The purpose of this study was to determine the characteristics of the dragon fruit peel extract Betacyanin Hylocereus polyrhizus and Hylocereus undatus as well as the stability of the organoleptic jelly, which will be developed into a learning materials atlas for class VIII Junior High School. The study was conducted in September-October 2015. The study was conducted in three stages. This type of research phase I and II is True Experimental, and phase III is development. The results of phase I shows that various concentrations of ethanol (70% and 90%) have an effect on the characteristics of the extract Betacyanin skin dragon fruit Hylocereus polyrhizus and Hylocereus undatus, but very significant effect on skin extract dragon fruit Hylocereus undatus the treatment of N2, EI at pH 4,5. Later in the phase II study results showed that different concentrations of extracts of the best Betacyanin significantly affect the organoleptic stability of jelly. The results of phase III is the development of phase I and II studies into Atlas media for 8th grade of  Junior High School.  

Flow Cytometrically Determined DNA Content of Breast Carcinoma and Benign Lesions: Correlations with Histopathological Parameters

1986 ◽  
Vol 72 (2) ◽  
pp. 171-177 ◽  
Author(s):  
Raffaella Uccelli ◽  
Alberto Calugi ◽  
Donato Forte ◽  
Francesco Mauro ◽  
Paolo Polonio-Balbi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Cell Subpopulation ◽  
Aneuploid Cell ◽  
Breast Carcinomas ◽  
Benign Lesions ◽  
Dna Index ◽  
Tissue Samples ◽  
Normal Tissues ◽  
Relative Dna Content

The relative DNA content of cellular samples from 54 patients affected by breast carcinomas and 20 affected by benign breast lesions (including 11 fibroadenomas) was measured by flow cytometry. All normal tissue samples and 17/20 (85%) specimens from benign lesions exhibited a cytometrically diploid DNA distribution, 3/20 (15%) benign lesions an abnormal DNA content, and 35/54 (65%) carcinomas at least one aneuploid cell subpopulation. Furthermore, 9/54 (17%) tumors were characterized by the presence of more than one aneuploid cell subpopulation. The results also indicate that flow cytometry can be used to recognize lymph nodes infiltrated by aneuploid cells. Statistically significant correlations were evidenced between the occurrence of aneuploidy or the ploidy level measured as DNA index and the nodal infiltration status. The percentage of S cells can also be extracted from DNA content distribution histograms. Statistically significant differences (p < 0.01) were also observed for the percentage of S cells between normal tissues (6.2±3.2 SD) and benign lesions (11.1±6.6 SD), normal tissues (6.2 ± 3.2 SD) and aneuploid tumors (19.7 ± 10.3 SD), benign lesions (11.1 ± 6.6 SD) and aneuploid tumors (19.7 ± 10.3 SD), and diploid (7.9 ± 4.0 SD) and aneuploid tumors (19.7 ± 10.3 SD).

scholarly journals In Vitro Inhibition of Fungal Antagonists on Fusarium sp., the Disease Causative Agent on Dragon Fruit Plant (Hylocereus undatus (Haw.) Britton & Rose)

2018 ◽  
Vol 5 (2) ◽  
pp. 224
Author(s):  
Dewa Ayu Andriastini ◽  
Yan Ramona ◽  
Meitini Wahyuni Proborini
Keyword(s):  
Causative Agent ◽  
Biocontrol Agent ◽  
Antagonistic Activity ◽  
Assay Method ◽  
In Vitro Growth ◽  
Dragon Fruit ◽  
Fusarium Sp ◽  
In Vitro Inhibition ◽  
Hylocereus Undatus

A research on in vitro inhibition of fungal antagonists, isolated from dragon fruit plantation in Sembung village, Bali, on Fusarium sp. (the disease causative agent of dragon fruit plant) was conducted with the main objective to investigate the effectiveness of these fungal antagonists to inhibit the in vitro growth of the pathogen. Dual assay method was applied in this experiment. The results showed that three potential fungal antagonists were successfully isolated in this research and they were identified as Trichoderma harzianum, Aspergillus niger, dan Paecilomyces lilacinus. All these fungal antagonists showed antagonistic activity against Fusarium sp. which was statistically significant (p<0.05) when compared to control. This indicated that all antagonist isolates were potential to be developed as biocontrol agent candidates.

Dietary dragon fruit (Hylocereus undatus) peel powder improved in vitro rumen fermentation and gas production kinetics

2019 ◽  
Vol 51 (6) ◽  
pp. 1531-1538 ◽  
Author(s):  
Maharach Matra ◽  
Metha Wanapat ◽  
Anusorn Cherdthong ◽  
Suban Foiklang ◽  
Chaowarit Mapato
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Production Kinetics ◽  
Dragon Fruit ◽  
Hylocereus Undatus ◽  
In Vitro Rumen Fermentation

In Vivo and In-Vitro Evaluation of Antimicrobial Activity of Peel Extracts of Red Dragon Fruit (Hylocereus polyrhizus)

2019 ◽  
Vol 11 (1) ◽  
pp. 23 ◽  
Author(s):  
Yogita Temak ◽  
Pravin Cholke ◽  
Akshay Mule ◽  
Akahay Shingade ◽  
Sudam Narote ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
In Vitro Evaluation ◽  
Dragon Fruit ◽  
Hylocereus Polyrhizus

scholarly journals Pertumbuhan Biji Buah Naga (Hylocereus Polyrhizus) dengan Pemberian NAA dan Ekstrak Biji Jagung (Zea Mays) secara In Vitro

2021 ◽  
Vol 11 (1) ◽  
pp. 47
Author(s):  
Rizka Widasari ◽  
Mukarlina Mukarlina ◽  
Zulfa Zakiah
Keyword(s):  
Seed Germination ◽  
Growth Regulators ◽  
Seed Extract ◽  
Cultivated Plants ◽  
Corn Seed ◽  
Germination Time ◽  
M 10 ◽  
Dragon Fruit ◽  
Hylocereus Polyrhizus

(Article History: Received 25 November 2020; Revised 5 January 2021; Accepted 10 February 2021) ABSTRAKBuah naga (Hylocereus polyrhizus) merupakan salah satu tanaman yang dibudidayakan di Indonesia. Perbanyakan bibit secara in vitro dengan teknologi kultur jaringan merupakan cara alternatif yang tepat untuk menyediakan bibit dalam waktu singkat dengan jumlah banyak. Penelitian ini bertujuan untuk menguji pengaruh dan konsentrasi terbaik penambahan ekstrak biji jagung dan NAA pada media tumbuh terhadap perkecambahan biji buah naga merah secara in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian menggunakan Rancangan Acak Lengkap dengan 2 faktor perlakuan. Faktor pertama yaitu NAA dengan konsentrasi 0 M, 10-8M, 10-7M, dan 5x10-7M. Faktor kedua yaitu ekstrak biji jagung dengan konsentrasi 0%, 5%, 7,5%, dan 10%. Hasil penelitian menunjukkan pemberian ekstrak biji jagung tunggal, NAA tunggal dan kombinasi ekstrak biji jagung dan NAA memberikan pengaruh nyata terhadap tinggi planlet, namun tidak berpengaruh nyata terhadap persentase perkecambahan. Pemberian ekstrak biji jagung tunggal memberikan pengaruh nyata terhadap waktu berkecambah, sedangkan pemberian ekstrak biji jagung tunggal dan NAA tunggal berpengaruh nyata terhadap jumlah akar. Perlakuan  5.10-7M NAA + 7,5% ekstrak biji jagung menghasilkan pertumbuhan tinggi plantlet terbaik. Konsentrasi 7,5% ekstrak biji jagung merupakan konsentrasi terbaik waktu berkecambahan biji buah naga dan konsentrasi NAA tunggal 10-8M memberikan jumlah akar terbanyak.Kata Kunci: Pertumbuhan in vitro, Biji Buah Naga, Ekstrak Biji Jagung, NAA.                                     ABSTRAKDragon fruit (Hylocereus polyrhizus) is one of the cultivated plants in Indonesia. In vitro propagation of seeds with the addition of synthetic growth regulators or from growth regulators from organic matter in the media, is an appropriate alternative way to provide large quantities of seeds in a short time. This study aims to determine the effect and the best effect of corn seed extract and NAA on growing media on in vitro red dragon fruit seed germination. The research was conducted at the Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Tanjungpura Pontianak. The study used a completely randomized design with 2 treatment factors. The first factor is NAA with a concentration of 0 M, 10-8M, 10-7M, and 5x10-7M. The second factor is corn seed extract with a concentration of 0%, 5%, 7.5%, and 10%. The results showed that the administration of single corn seed extract, single NAA and a combination of corn seed extract and NAA had a significant effect on plantlet height, but did not significantly affect the germination percentage. The administration of single corn seed extract had a significant effect on germination time, while the administration of single corn seed extract and single NAA had a significant effect on the number of roots. The 5.10-7M NAA + 7.5% corn seed extract treatment resulted in the best plantlet height growth. The concentration of 7.5% corn seed extract was the best concentration for dragon fruit seed germination time and a single NAA concentration of 10-8M gave the highest number of roots.Keywords: In Vitro Growth, Dragon Fruit Seeds, Corn Seed Extract, NAA.

scholarly journals Tumor-tumor Cell Fusion Hybrids Obtained by the Modified PHA-DMSO-PEG Method Promote Temozolomide Resistance in Glioma

2021 ◽  
Author(s):  
Ruifang Mi ◽  
Jiayu Ji ◽  
Mingxin Li ◽  
Mengmeng Zhang ◽  
Junwen Zhang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cell ◽  
Cell Fusion ◽  
Dna Content ◽  
Glioma Cell ◽  
Glioma Cells ◽  
Giant Cells ◽  
Fusion Method ◽  
Fusion Efficiency

Abstract Background: Cell fusion and the subsequent aneuploidy are commonly observed in different kinds of tumor. In glioma, cell fusion and the number of the polyploid giant cells were found to be augmented with the tumor grades (WHO Ⅰ-Ⅳ) and closely related to poor prognosis. Phytohemagglutinin (PHA) holds an ability to induce cell-cell membrane contact and accelerates the cell fusion process mediated by the fusogenic agent polyethylene glycol (PEG). Dimethyl sulfoxide (DMSO) is well known as a cryoprotective agent involving in cell cryopreservation. In this study, we aim to obtain the glioma fusion hybrids by the modified fusion method in vitro, and then investigate the pathological consequences and the related molecular mechanism with the cell hybrids.Methods: Glioma cells were labelled by lentiviruses infection. The PEG fusion efficiency was respectively improved by the addition of PHA and DMSO, and quantified by flow cytometry. Then, fusion hybrids were obtained by puromycin screening and fluorescence-activated cell sorting (FACS). Furthermore, DNA content was analyzed through flow cytometry. Cell proliferation rate and cell viability under temozolomide (TMZ) was detected by CCK-8 assay. Lastly, the related gene expression was measured through qRT-PCR and Western blotting. Results: Glioma cell-cell contact was achieved by adding certain concentration of PHA in vitro. Tumor-tumor cell fusion efficiency was improved by PHA and DMSO. Glioma fusion hybrids were successfully obtained after puromycin screening and FACS. Cell size, DNA content and chromosome numbers of the fusion hybrids were almost twice that of the parental glioma cells. Moreover, glioma fusion hybrids showed an enhanced TMZ resistance potential compared to the parental cells, and also the MGMT expression was up-regulated in the hybrids.Conclusions: We successfully obtained the glioma tumor-tumor cell fusion hybrids through the modified PHA-DMSO-PEG fusion method. Cell fusion may contribute to TMZ resistance in glioma, thus inhibition of cell fusion could be a promising orientation to improve TMZ resistance. Moreover, combining TMZ and MGMT inhibitor could be a beneficial approach in patients with glioma polyploid giant cells.

scholarly journals Brain-Derived Microparticles Induce Systemic Coagulation Associated with Traumatic Brain Injury

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 1497-1497
Author(s):  
Jing-fei Dong ◽  
Ye Tian ◽  
Breia Salsbery ◽  
Hengjie Yuan ◽  
Min Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Traumatic Brain Injury ◽  
Flow Cytometry ◽  
Coagulation Factors ◽  
Endothelial Barrier ◽  
Dependent Manner ◽  
Activated Platelets ◽  
Dose Dependent ◽  
Made In

Abstract Uncontrolled hemorrhage is a leading cause of the preventable deaths that occur in patients with trauma. The cause of trauma-associated coagulopathy is multifactorial, including blood loss, consumption of coagulation factors and platelets, the dilution of coagulation factors and platelets due to fluid resuscitation, and hypothermia. Traumatic brain injury (TBI) lacks two key causal factors for coagulopathy: heavy blood loss and a large volume of fluid resuscitation, but is associated with a significantly higher incidence of coagulopathy. The pathogenesis of this TBI-associated coagulopathy remains poorly understood. We tested the hypothesis that brain-derived microparticles (BDMPs) released from an injured brain play a causal role in developing systemic coagulopathy after TBI. Here, we report that mice subjected to fluid percussion injury (1.9±0.1 atm) developed a BDMP-dependent hypercoagulable state, with a peak level of plasma glial cell and neuronal microparticles, reaching 17,496 ± 4,833/µl and 18,388 ± 3,657/µl 3 hrs after TBI. BDMPs were measured by flow cytometry using triple gating based on particle size and the expression of neural cell markers and phosphatidylserine (PS). To exclude contributions to the coagulopathy of non-neural cell microparticles released during trauma stress, BDMPs were made from normal brain by freeze-thawing and mechanical injury. BDMPs thus made had below detection levels of microparticles from leukocytes (CD45), endothelial cells (CD144), erythrocytes (CD235a), and platelets (CD42b). Uninjured mice injected with BDMPs made in vitro developed a hyper-turn-hypo-coagulable state in a dose-dependent manner as measured by the rates of clot formation and fibrinogen depletion, resulting in microvascular fibrin deposition in the lungs, kidney and heart. BDMPs measured 50 – 500 nm with relatively intact membranes under transmission electron microscopy and expressed neuronal or glial cell markers and procoagulant PS and tissue factor (TF). BDMPs promoted clot formation in a PS-dependent assay at a maximal activity of ~1 x 105 BDMPs/µl, equivalent to 1.6 µg/µl of purified brain PS. They were equally active in promoting thrombin generation in a PS-and TF-dependent manner, BDMPs at 2.5 x 104 /µl yielding an activity equivalent to 1 pM of soluble TF. The procoagulant activity of BDMPs was significantly stronger than microparticles generated from collagen-stimulated platelets and was blocked by the PS-binding lactadherin in a dose-dependent manner. Consistent with observations made in the mouse models, fetal hippocampal cells in culture produced microparticles upon injury. These microparticles transmigrated through the disrupted endothelial barrier in the presence of live, but not lyophilized platelets. BDMP-bound platelets were detected by flow cytometry and scan electron microscopy. They activated platelets as measured by increases in calcium influx and CD62p expression, but did not induce platelet aggregation directly or in the presence of low doses of collagen. In summary, we have studied acute changes in coagulation associated with TBI using a mouse FPI model combined with in vitro experiments. Focusing on the first 6 hrs post-TBI minimizes confounding changes induced by secondary events, such as ischemic injury. The results define a causal role for BDMPs in the TBI-associated systemic coagulation. We also show that BDMPs activated platelets. Activated platelets may facilitate the transmigration of BDMPs through the disrupted endothelial barrier by releasing pro-inflammatory mediators to promote local inflammation at a site of vascular injury. This notion is supported by the finding that live, but not lyophilized platelets and, to lesser degree, plasma from activated platelets promoted BDMP transmigration through a monolayer of endothelial cells. Finally, the PS binding lactadherin blocked the BDMP-dependent procoagulant activity, raising two interesting perspectives. First, PS scavengers and neutralizing molecules may reduce or prevent coagulopathy associated with TBI. Second, an intrinsic or acquired deficiency in the PS-dependent clearance of microparticles may predispose an individual to consumptive coagulopathy associated with TBI and other conditions. Disclosures No relevant conflicts of interest to declare.

scholarly journals MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT

2019 ◽  
Vol 6 (1) ◽  
pp. 1-7
Author(s):  
Diego Pandeló José ◽  
José Marcello Salabert De Campos ◽  
Lyderson Facio Viccini ◽  
Emilly Ruas Alkimim ◽  
Marcelo De Oliveira Santos
Keyword(s):  
Flow Cytometry ◽  
Medicinal Plant ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Naphthalene Acetic Acid ◽  
Flow Cytometry Analysis ◽  
Ploidy Levels

Lippia lacunosa is a Brazilian savanna plant that belongs to the Verbenaceae family. It has been used in folk medicine as a treatment for different diseases. This species represents an endangered Brazilian medicinal plant, and this is the first report documenting a reliable protocol for the in vitro propagation and regeneration of L. lacunosa. Axenic explants were cultivated in MS medium containing different concentrations of naphthalene acetic acid (NAA) to induce root growth. The mean shoot length and the number of roots were highest with 0.06 mg·L-1 NAA. The highest number of buds in shoot regeneration was induced with 2 mg·L-1 6-benzylaminopurine (BA). To obtain a long-term culture, the dwarf shoots were elongated on MS media containing 0.5 mg·L-1 BA alternated with MS containing 2 mg·L-1 BA every 40 days. In the present protocol, the long-term shoots retained the ability to root even after long periods of BA treatment. In addition, we evaluated the nuclear DNA content and ploidy levels, including the occurrence of endopolyploidy, in long-term micropropagated plant leaves using flow cytometry analysis. The plants propagated in vitro over several years possessed nuclear DNA contents ranging from 2.940 to 3.095 pg, and no differences in DNA content were found among in vitro plants or between these plants and the control (L. lacunosa from a greenhouse with a DNA content of 3.08 pg). The flow cytometry analysis also demonstrated that there was no polyploidization. The present study will be useful for biotechnological approaches and provides the first estimate of the nuclear DNA content of this species using flow cytometry.

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