In vitro activity of zinc oxide-eugenol and glass ionomer cements on Candida albicans

The aim of this study was to evaluate in vitro the antimicrobial activity of glass ionomer (GIC) and zinc oxide-eugenol (ZOE) cements against Candida albicans. Standardized GIC and ZOE specimens were maintained in contact with C. albicans suspension (1 <FONT FACE=Symbol>´</FONT> 10(6) cells/ml) at 37°C for 24 h, 48 h or 7 days. A control group without any testing cement was included. After the incubation period, aliquots of 0.1 ml were plated on Sabouraud's agar, and then the number of colonies was counted. The results were expressed as values of logarithms of colony-forming units per milliliter (log CFU/mL) and were analyzed statistically by Kruskal-Wallis ANOVA. After 48 h of incubation, the ZOE group presented no growth of C. albicans. GIC and control groups presented similar mean values at all tested periods. According to the results obtained, it could be concluded that, under the experimental conditions, ZOE cement was more effective in vitro against C. albicans than GIC.

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Marginal Leakage of Endodontic Temporary Restorative Materials around Access Cavities Prepared with Pre-Endodontic Composite Build-Up: An In Vitro Study

Materials ◽

10.3390/ma13071700 ◽

2020 ◽

Vol 13 (7) ◽

pp. 1700

Author(s):

Atsushi Kameyama ◽

Aoi Saito ◽

Akiko Haruyama ◽

Tomoaki Komada ◽

Setsuko Sugiyama ◽

...

Keyword(s):

Zinc Oxide ◽

Resin Composite ◽

Glass Ionomer Cement ◽

In Vitro Study ◽

Restorative Material ◽

Glass Ionomer ◽

Zinc Oxide Eugenol ◽

Restorative Materials ◽

Ionomer Cement

This study aimed to examine the marginal seal between various commercial temporary restorative materials and exposed dentin/built-up composite. Sixty bovine incisors were cut above the cemento-enamel junction, and half of the dentin was removed to form a step, which was built up using flowable resin composite. The root canals were irrigated, filled with calcium hydroxide, and sealed using one of six temporary sealing materials (hydraulic temporary restorative material, temporary stopping material, zinc oxide eugenol cement, glass-ionomer cement, auto-cured resin-based temporary restorative material, and light-cured resin-based temporary restorative material) (n = 10 for each material). The samples were thermocycled 500 times and immersed in an aqueous solution of methylene blue. After 2 days, they were cut along the long axis of the tooth and the depth of dye penetration was measured at the dentin side and the built-up composite side. For the margins of the pre-endodontic resin composite build-up, the two resin-based temporary restorative materials showed excellent sealing. Hydraulic temporary restorative material had a moderate sealing effect, but the sealing effect of both zinc oxide eugenol cement and glass-ionomer cement was poorer.

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Retentiveness of Various Luting Agents Used With Implant-Supported Prosthesis: An In Vitro Study

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-12-00008 ◽

2014 ◽

Vol 40 (6) ◽

pp. 649-654 ◽

Cited By ~ 8

Author(s):

Pooja Garg ◽

Malesh Pujari ◽

D. R. Prithviraj ◽

Sumit Khare

Keyword(s):

Zinc Oxide ◽

Zinc Phosphate ◽

Glass Ionomer Cement ◽

Glass Ionomer ◽

Mean Values ◽

Pull Out ◽

Zinc Oxide Eugenol ◽

Bonferroni Test ◽

The Mean ◽

Ionomer Cement

Desired retrievability of cemented implant-supported fixed prosthesis makes the retentive strength of cementing agents an important consideration. The aim of the study was to evaluate the retentiveness of purposely designed implant cement and compare its retentiveness with dental cements that are commonly used with implant systems. Ten implant analogs were embedded in auto-polymerizing acrylic resin blocks and titanium abutments were attached to them. Fifty standardized copings were waxed directly on the abutment and casted. The cements used were: (1) resin-bonded zinc oxide eugenol cement, (2) purposely designed implant cement, (3) zinc phosphate cement, (4) zinc polycarboxylate cement, and (5) glass ionomer cement. After cementation, each sample was subjected to a pull-out test using universal testing machine and loads required to remove the crowns were recorded. The mean values and standard deviations of cement failure loads were analyzed using ANOVA and Bonferroni test. The mean values (± SD) of loads at failure (n = 10) for various cements were as follows (N): resin-bonded zinc oxide eugenol cement 394.62 (± 9.76), Premier implant cement 333.86 (± 18.91), zinc phosphate cement 629.30 (± 20.65), zinc polycarboxylate cement 810.08 (± 11.52), and glass ionomer cement 750.17 (± 13.78). The results do not suggest that one cement type is better than another, but they do provide a ranking order of the cements regarding their ability to retain the prosthesis and facilitate easy retrievability.

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In vitro Evaluation of Topical Fluoride Varnishes Effect on Enamel Demineralization Around Orthodontic Brackets

Revista de Chimie ◽

10.37358/rc.18.11.6673 ◽

2018 ◽

Vol 69 (11) ◽

pp. 3013-3017

Author(s):

Roxana Oancea ◽

Anca Mesaros ◽

Octavia Iulia Balean ◽

Angela Codruta Podariu

Keyword(s):

Resin Composite ◽

Glass Ionomer Cement ◽

Orthodontic Brackets ◽

Fluoride Varnish ◽

Control Group ◽

Glass Ionomer ◽

Mean Values ◽

Resin Modified Glass Ionomer ◽

Ionomer Cement

The aim of this in vitro study was to test the effectiveness of applying the fluoride varnish on bracketed teeth with 2 types of bonding agent. 10 patients aged 12-18 (mean age 13.06 +/- 1.72 years) were scheduled to have the four fist premolar teeth extracted. The teeth were dived in 4 groups: 2 experimental and 2 controls. The teeth were cut in half so 80 surfaces were analyzed. The brackets were collated with Transbond XT and Fuji Ortho Lc. For each of the materials that have been used there was a test and a control group. The tests groups received one single application of Duraphat. The content in fluoride was measured by spectrophotometer. The mean values of fluoride indicated an important increase in the fluoride content after using resin modified glass ionomer cement and fluoride varnish. Statistically significant differences were determined between each of the control and treatment group when both materials have been used, after 10 days and one month application of the varnish. The study pointed out that the fluoride varnish combined with resin modified glass ionomer cement is more efficient than the classical resin composite in preventing demineralization around orthodontic brackets.

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Photodynamic inactivation of planktonic cultures of Streptococcus mutans using erythrosine irradiated by LED

Brazilian Dental Science ◽

10.14295/bds.2020.v23i2.1857 ◽

2020 ◽

Vol 23 (2) ◽

Author(s):

Maria Ângela Lacerda Rangel Esper ◽

Junia Oliveira Barbosa ◽

Janaína De Araújo Alvarenga ◽

Juliana Campos Junqueira ◽

Alessandra nara De Souza Rastelli ◽

...

Keyword(s):

Streptococcus Mutans ◽

Light Emitting Diode ◽

In Vitro Study ◽

Control Group ◽

Photodynamic Inactivation ◽

Experimental Conditions ◽

Light Emitting ◽

Colony Forming Units ◽

Led Irradiation

Objective: The aim of this in vitro study was to evaluate the efficacy of photodynamic inactivation (PDI) with erythrosine (E), using a light-emitting diode (LED) on planktonic cultures of Streptococcus mutans. Material and Methods: A Streptococcus mutans strain (UA 159) was used to prepare the suspensions containing 107 cells/mL, which was tested under different experimental conditions: a) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); b) LED irradiation only (P-L+); c) treatment with erythrosine only (E+L-); and d) no LED irradiation or photosensitizer (P) treatment, which served as a control group (P-L-). After treatment, strains were seeded onto MSBS agar for determination of the number of colony-forming units (CFU/mL). Results: The results were submitted to analysis of variance and the Tukey test (p < 0.05). No reduction in the number of CFU/mL was observed in the treatment group with erythrosine (E+L+) when compared to the control (P-L-). Conclusion: PDI using erythrosine did not reduce the number of CFUs per millimeter within the parameters in this study.KEYWORDSErythrosine; Decay; Photodynamic inactivation; Light-emitting diode.

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Agitation Techniques to Enhance Drainage of Retained Hemothorax

Surgical Innovation ◽

10.1177/1553350620978002 ◽

2020 ◽

pp. 155335062097800

Author(s):

Ian A. Makey ◽

Nitin A. Das ◽

Samuel Jacob ◽

Magdy M. El-Sayed Ahmed ◽

Colleen M. Makey ◽

...

Keyword(s):

Trauma Surgery ◽

Control Group ◽

In Vivo Experiments ◽

Vibration Technique ◽

Retained Hemothorax ◽

And Control ◽

Negative Conclusion ◽

Benchtop Model

Background. Retained hemothorax (RH) is a common problem in cardiothoracic and trauma surgery. We aimed to determine the optimum agitation technique to enhance thrombus dissolution and drainage and to apply the technique to a porcine-retained hemothorax. Methods. Three agitation techniques were tested: flush irrigation, ultrasound, and vibration. We used the techniques in a benchtop model with tissue plasminogen activator (tPA) and pig hemothorax with tPA. We used the most promising technique vibration in a pig hemothorax without tPA. Statistics. We used 2-sample t tests for each comparison and Cohen d tests to calculate effect size (ES). Results. In the benchtop model, mean drainages in the agitation group and control group and the ES were flush irrigation, 42%, 28%, and 2.91 ( P = .10); ultrasound, 35%, 27%, and .76 ( P = .30); and vibration, 28%, 19%, and 1.14 ( P = .04). In the pig hemothorax with tPA, mean drainages and the ES of each agitation technique compared with control (58%) were flush irrigation, 80% and 1.14 ( P = .37); ultrasound, 80% and 2.11 ( P = .17); and vibration, 95% and 3.98 ( P = .06). In the pig hemothorax model without tPA, mean drainages of the vibration technique and control group were 50% and 43% (ES = .29; P = .65). Discussion. In vitro studies suggested flush irrigation had the greatest effect, whereas only vibration was significantly different vs the respective controls. In vivo with tPA, vibration showed promising but not statistically significant results. Results of in vivo experiments without tPA were negative. Conclusion. Agitation techniques, in combination with tPA, may enhance drainage of hemothorax.

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How Downward and Upward Comparisons on Facebook Influence Grandiose and Vulnerable Narcissists’ Self-Esteem—A Priming Study

Behavioral Sciences ◽

10.3390/bs11030039 ◽

2021 ◽

Vol 11 (3) ◽

pp. 39

Author(s):

Phillip Ozimek ◽

Hans-Werner Bierhoff ◽

Elke Rohmann

Keyword(s):

Social Networking Sites ◽

Online Survey ◽

Social Comparisons ◽

Past Research ◽

Self Esteem ◽

Self Report ◽

Control Group ◽

Experimental Conditions ◽

Vulnerable Narcissism ◽

And Control

Past research showed that social networking sites represent perfect platforms to satisfy narcissistic needs. The present study aimed to investigate how grandiose (GN) and vulnerable narcissism (VN) as well as social comparisons are associated with Facebook activity, which was measured with a self-report on three activity dimensions: Acting, Impressing, and Watching. In addition, the state self-esteem (SSE) was measured with respect to performance, social behavior, and appearance. One hundred and ten participants completed an online survey containing measures of SSE and Facebook activity and a priming procedure with three experimental conditions embedded in a social media context (upward comparison, downward comparison, and control group). Results indicated, as expected, that high VN was negatively associated with SSE on each subscale and the overall score. In addition, it was found that VN, but not GN, displayed positive associations with frequency of Facebook activities. Finally, it was proposed and confirmed that VN in interaction with the priming of downward comparisons negatively affected SSE. The conclusion drawn is that VN represents a key variable for the prediction of self-esteem as well as for the frequency of Facebook activity.

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THE UPTAKE OF TRITIATED LYSINE VASOPRESSIN BY RAT AND PIG PITUITARY NEURAL LOBES IN VITRO

Journal of Endocrinology ◽

10.1677/joe.0.0500669 ◽

1971 ◽

Vol 50 (4) ◽

pp. 669-677 ◽

Cited By ~ 4

Author(s):

B. A. EDWARDS

Keyword(s):

Tap Water ◽

Exchange Chromatography ◽

Control Group ◽

Breakdown Product ◽

Nacl Solution ◽

Water Control ◽

Neural Lobe ◽

Lysine Vasopressin ◽

And Control

SUMMARY Uptake of tritiated lysine vasopressin ([3H]LVP) was studied in halved neural lobes of rats (which had been given either tap water (control group) or 2% (w/v) NaCl solution as drinking water for 4 days) as well as in slices of pig neural lobe. Uptake of radioactivity into the neural lobes was shown but analysis of the extracts of incubated lobes of both species by ion exchange chromatography showed that very little of it remained in the tissue as hormone. In addition, some radioactivity was associated with trichloroacetic acid-insoluble proteins. After 90 min of incubation, and after correction for the breakdown, the uptake of unchanged [3H]LVP, expressed as a tissue: medium ratio, was 0·14 ± 0·04 and 0·09 ± 0·03 (mean ± s.e.m.) for the saline-treated and control rats respectively, while the tissue: medium ratios for the breakdown product(s) were 6·47 ± 0·45 and 5·50 ± 0·36. The results suggest uptake of [3H]LVP into the cell with almost complete intracellular breakdown of the hormone.

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The flow of two zinc oxide-eugenol-based endodontic sealers

Vojnosanitetski pregled ◽

10.2298/vsp1301021i ◽

2013 ◽

Vol 70 (1) ◽

pp. 21-25

Author(s):

Dragan Ilic

Keyword(s):

Zinc Oxide ◽

Flow Rate ◽

Null Hypothesis ◽

Applied Force ◽

National Standard ◽

Control Group ◽

Disk Diameter ◽

Zinc Oxide Eugenol ◽

Significant Difference ◽

Endodontic Sealers

Background/Aim. Endodontic sealers (ES) for obturation are usually prepared with a slight variation of their components both on purpose or unintentionally. Considering that fact, as well as a frequent use of compaction techniques with the applied force to gutta-percha and ES of 1-3 kg, the aim of this study was to investigate the flow of two zinc-oxide eugenol ES in regard to the applied force and a variation of sealer?s components. Methods. The experimental group samples of both ES were prepared according to the manufacturer?s instructions, applied between pair of glass slabs and loaded by weights of 1 and 2 kg, respectively (American National Standard, Specification No. 57). Some samples of one ES were prepared as thick consistency with 10% more powder and some as thin mixture with 10% less powder than the standard prescription. These semples had been exposed to the load of 2 kg. The control group included samples of both ES prepared as standard prescription but exposed to the weight of one glass slab only. The spread ES appeared as a regular circle 10 min upon mixing and weighting. Measuring of the circle diameter was done by an orthodontic ruler. The flow of the used ES was considered the function of its spread diameter. Results. Application of 1 vs 2 kg load for both regularly mixed sealers in the scope of disk diameter (flow) was statistically insignificant (p > 0.05). This means that the stated null hypothesis that there would be no significant difference in flow rate among the regularly mixed sealers at the level of ? = 0.05 is accepted. The findings about difference in the disk diameter in regard to mixing variation of Endomethasone indicate that the null hypothesis that there would be no significant difference in flow rate between the regular and thick mixed mass at the level of ? = 0.05 is accepted. In the comparison of regular and thin mix a significant difference was noted and the null hypotesis is rejected (p < 0.01). The control group results displayed Roth 801 as less viscous than Endomethasone sealer (p < 0.01). Conclusion. Application of 1 or 2 kg pressure on the samples of both exposed sealers does not significantly affect the flow values as well as comparison of the regular to thick consistency of Endomethasone while comparison of its regular to thin mass shows a significant difference.

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136 EVIDENCE OF A DIRECT EFFECT OF P4 ON IVF-DERIVED BOVINE 8-CELL EMBRYOS

Reproduction Fertility and Development ◽

10.1071/rdv17n2ab136 ◽

2005 ◽

Vol 17 (2) ◽

pp. 219 ◽

Cited By ~ 5

Author(s):

C.E. Ferguson ◽

T.R. Davidson ◽

M.R.B. Mello ◽

A.S. Lima ◽

D.J. Kesler ◽

...

Keyword(s):

Embryo Development ◽

Direct Effect ◽

Blastocyst Stage ◽

Control Group ◽

Bovine Embryo ◽

Bovine Embryos ◽

Direct Role ◽

Treatment Groups ◽

And Control

There has been much debate over a direct role for progesterone (P4) in early bovine embryo development. While previous attempts to supplement bovine embryos in vitro with P4 produced results that vary and are often contradictory, this may be a response of administering P4 at inappropriate times. Therefore, the objective of these experiments was to determine if P4 could exert a direct effect on developing IVF-derived bovine embryos when administered at an appropriate time of embryo development. In Exp. I, IVF-derived bovine 8-cell embryos were randomly allotted to treatments: (1) control, CR1aa medium (n = 168); (2) vehicle, CR1aa + ETOH (0.01%) (n = 170); and (3) P4, CR1aa + ETOH + P4 (20 ng/mL in 50-μL droplet) (n = 173). In Exp. II, IVF-derived bovine 8-cell embryos were randomly allotted to treatments: (1) control, CR1aa medium (n = 160); (2) vehicle, CR1aa + DMSO (0.01%) (n = 180); and (3) P4, CR1aa + DMSO (0.01%) + P4 (20 ng/mL in 50-μL droplet) (n = 170). All embryos were evaluated on Days 6 to 9 post-insemination and rates calculated from 8-cell embryos. In Exp. I, ETOH tended to have a detrimental effect with significantly fewer (P < 0.05) embryos (53%) developing to the blastocyst stage on Day 7 compared with the control (62%) and P4 (71%) groups. At Day 7, significantly more embryos cultured in P4 (71%) developed to the blastocyst stage compared with the control group (62%). P4 treatment significantly increased the number of Grade 1 blastocysts (25%) on Day 7 compared with vehicle (15%) and control (17%) groups. At the end of culture, there were also significantly more Day 9 hatched blastocysts in the P4 group (33%) compared with vehicle (22%) and control (21%) groups. Supplementing P4 in the culture medium increased the rate of development, resulting in significantly more blastocysts (8%) on Day 6 and hatched blastocysts (21%) on Day 8 compared with vehicle (3% and 12%) and control (0% and 8%) groups, respectively. In Exp. II, there were no significant differences between treatment groups for Day 7 blastocysts (control 54%, DMSO 61%, P4 57%) and Day 9 hatched blastocysts (control 46%, DMSO 51%, P4 46%). However, there were significantly more Grade 1 blastocysts in the P4 group (22% and 36%) on Days 6 and 8 compared with vehicle (11% and 23%) and control (13% and 23%) groups, respectively. The lack of improvement in Day 7 blastocysts and Day 9 hatched blastocysts rates leads to further uncertainty in understanding the P4 vehicle interactions. In conclusion, the results of these two experiments indicate that P4 can exert a direct effect on the developing IVF-derived bovine embryo; however, due to P4 vehicle interactions; other inert vehicles need to be explored to further evaluate the direct effects of P4 on the developing bovine embryo.

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The Accuracy and Reliability of Tooth Shade Selection Using Different Instrumental Techniques: An In Vitro Study

Sensors ◽

10.3390/s21227490 ◽

2021 ◽

Vol 21 (22) ◽

pp. 7490

Author(s):

Nattapong Sirintawat ◽

Tanyaporn Leelaratrungruang ◽

Pongsakorn Poovarodom ◽

Sirichai Kiattavorncharoen ◽

Parinya Amornsettachai

Keyword(s):

Intraclass Correlation ◽

In Vitro Study ◽

Control Group ◽

Intraoral Scanner ◽

Control Groups ◽

B Values ◽

Cie Lab ◽

And Control ◽

Shade Selection

This study aimed to investigate and compare the reliability and accuracy of tooth shade selection in the model using 30 milled crowns via five methods: (1) digital single-lens reflex (DSLR) camera with twin flash (TF) and polarized filter (DSLR + TF), (2) DSLR camera with a ring flash (RF) and polarized filter (DSLR + RF), (3) smartphone camera with light corrector and polarized filter (SMART), (4) intraoral scanner (IOS), and (5) spectrophotometer (SPEC). These methods were compared with the control group or manufacturer’s shade. The CIE Lab values (L, a, and b values) were obtained from five of the methods to indicate the color of the tooth. Adobe Photoshop was used to generate CIE Lab values from the digital photographs. The reliability was calculated from the intraclass correlation based on two repetitions. The accuracy was calculated from; (a) ΔE calculated by the formula comparing each method to the control group, (b) study and control groups were analyzed by using the Kruskal–Wallis test, and (c) the relationship between study and control groups were calculated using Spearman’s correlation. The reliability of the intraclass correlation of L, a, and b values obtained from the five methods showed satisfactory correlations ranging from 0.732–0.996, 0.887–0.994, and 0.884–0.999, respectively. The ΔE from all groups had statistically significant differences when compared to the border of clinical acceptance (ΔE = 6.8). The ΔE from DSLR + TF, DSLR + RF, SMART, and SPEC were higher than clinical acceptance (ΔE > 6.8), whereas the ΔE from IOS was 5.96 and all of the L, a, and b values were not statistically significantly different from the manufacturer’s shade (p < 0.01). The ΔE of the DSLR + RF group showed the least accuracy (ΔE = 19.98), whereas the ∆E of DSLR + TF, SMART, and SPEC showed similar accuracy ∆E (ΔE = 10.90, 10.57, and 11.57, respectively). The DSLR camera combined with a ring flash system and polarized filter provided the least accuracy. The intraoral scanner provided the highest accuracy. However, tooth shade selection deserves the combination of various techniques and a professional learning curve to establish the most accurate outcome.

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