scholarly journals Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

2018 ◽  
Author(s):  
Renata Cristina Ferreira Dias ◽  
Vanete Thomaz-Soccol ◽  
Aline Kuhn Sbruzzi Pasquali ◽  
Silvana Maria Alban ◽  
Ricardo Cancio Fendrich ◽  
...  
Keyword(s):  
Rapid Test ◽  
Immunofluorescence Assay ◽  
Confirmatory Test ◽  
Serum Samples ◽  
Leishmania Spp ◽  
Confirmatory Analysis ◽  
The City ◽  
Public Collection ◽  
Geographical Characteristics ◽  
Immunoenzymatic Assay

Abstract The aim of this study was to investigate the occurrence of anti-Leishmania spp. antibodies in dogs from localities in the city of Foz do Iguaçu, Paraná state, Brazil, on the border with Argentina and Paraguay. Blood samples dogs were collected to perform the following serologic tests: immunochromatographic DPP® rapid test, indirect immunoenzymatic assay (ELISA) and indirect immunofluorescence assay (IFA). In 2012, 285 dogs were analyzed on Argentina border, and in 2013, serum samples from 396 dogs on the border of Paraguay were collected. Using ELISA for screening and IFA for the confirmatory test, the results showed that the antibody prevalence was 1.8% (5/285) on the border of Argentina and 3.0% (12/396) on Paraguay border. When using the DPP® for screening and ELISA as a confirmatory analysis, we observed a seroreagent prevalence in dogs of 2.5% (7/285) on Argentina border and 5.1% (20/396) on Paraguay border. The non-public collection of domestic waste (p= 0.0004) was shown to be associated with leishmaniasis. This study shows the presence of leishmaniasis and suggest the emergence of canine visceral leishmaniasis in state of Paraná due to the confirmed occurrence of seroreactive dogs on Argentina and Paraguay border, which has environmental and geographical characteristics that favor the spread of the parasite.

scholarly journals Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology

2015 ◽  
Vol 22 (10) ◽  
pp. 1130-1132 ◽  
Author(s):  
Hugh W. F. Kingston ◽  
Stuart D. Blacksell ◽  
Ampai Tanganuchitcharnchai ◽  
Achara Laongnualpanich ◽  
Buddha Basnyat ◽  
...  
Keyword(s):  
Rapid Diagnostic Test ◽  
Scrub Typhus ◽  
Specific Antigen ◽  
Rapid Test ◽  
Febrile Illness ◽  
High Specificity ◽  
Immunofluorescence Assay ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Comparative Accuracy

ABSTRACTThis study investigated the comparative accuracy of a recombinant 56-kDa type-specific antigen-based rapid diagnostic test (RDT) for scrub typhus for the detection of IgM antibodies by using conventional serology in well-characterized serum samples from undifferentiated febrile illness patients. The RDT showed high specificity and promising comparative accuracy, with 82% sensitivity and 98% specificity for samples defined positive at an IgM indirect immunofluorescence assay positivity cutoff titer of ≥1:1,600 versus 92% and 95% at ≥1:6,400, respectively.

scholarly journals Serosurvey of tick-borne pathogens in dogs from urban and rural areas from Parana State, Brazil

2013 ◽  
Vol 22 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Thállitha Samih Wischral Jayme Vieira ◽  
Rafael Felipe da Costa Vieira ◽  
Denise Amaral Gomes do Nascimento ◽  
Kátia Tamekuni ◽  
Roberta dos Santos Toledo ◽  
...  
Keyword(s):  
Rural Areas ◽  
Urban Areas ◽  
Rapid Test ◽  
Immunofluorescence Assay ◽  
Human Infection ◽  
Risk Exposure ◽  
Serum Samples ◽  
Babesia Vogeli ◽  
Tick Borne Disease ◽  
Urban And Rural Areas

Considering the zoonotic potential of tick-borne disease (TBD) agents and the fact that dogs may act as sentinels for human infection, the aim of the present study was to determine the seroprevalence of TBD agents and risk factors for exposure in two different canine populations from Parana State, Southern Brazil. A total of 138 dog serum samples from urban (UA) (n=68) and rural (RA) (n=70) areas were tested with commercial ELISA rapid test forAnaplasma phagocytophilum, Ehrlichia canisand Borrelia burgdorferi antibodies and indirect immunofluorescence assay (IFAT) for Babesia vogeli. An overall of 92∕138 (66.7%) dogs, being 62∕68 (91.2%) from UA and 30∕70 (42.9%) from RA, were seropositive for at least one TBD agent. From the total number of dogs, sixty-two were positive for E. canis (44.9%), 19 (13.8%) for A. phagocytophilum, and 64 (46.4%) for B. vogeli. Anti-B. burgdorferi antibodies were not detected. Dogs from UA showed a higher percentage of tick infestation (p = 0.0135) and were highly associated with seropositivity to E. canis (p = 0.000005), A. phagocytophilum (p = 0.0001), and B. vogeli (p = 0.0012). In summary, the findings indicate that dogs from urban areas present higher potential risk exposure to TBD pathogens than those from rural areas.

scholarly journals Prevalence and risk factors associated with Leishmania spp. and Trypanosoma cruzi infections in dogs presented at veterinary clinics in João Pessoa, Paraíba state, northeastern Brazil

2018 ◽  
Vol 39 (5) ◽  
pp. 2293
Author(s):  
Arthur Willian de Lima Brasil ◽  
Dália Monique Ribeiro Machado ◽  
Mariana Aimee Ramos Xavier da Silva ◽  
Noelle De Castro Barrosa ◽  
Raizza Barros Sousa Silva ◽  
...  
Keyword(s):  
Risk Factors ◽  
Trypanosoma Cruzi ◽  
Fluorescent Antibody ◽  
Significant Proportion ◽  
Antibody Test ◽  
Northeastern Brazil ◽  
Confirmatory Test ◽  
Factors Associated ◽  
Leishmania Spp ◽  
The City

This study aimed to determine the prevalence and risk factors associated with Leishmania spp. and Trypanosoma cruzi infections in dogs presented at veterinary clinics in the city of João Pessoa, Paraíba state, northeastern Brazil. A total of 384 dogs from 34 veterinary clinics were sampled between April 2015 and May 2016. For serological diagnosis of canine visceral leishmaniasis and Chagas disease, the indirect fluorescent antibody test was used. Reactive sera were submitted to a confirmatory test using the S7 ELISA. The seroprevalence of Leishmania spp. was 2.8% (11/384), and that of T. cruzi was 1.5% (6/384). Two animals were positive for both parasites. The variable “access to the street” was identified as a risk factor for Leishmania spp. infection (OR = 4.81; 95% CI = 1.23–18.75). We concluded that a significant proportion of dogs presented at veterinary clinics in João Pessoa are seropositive for Leishmania spp. or T. cruzi infections, and we suggest that greater care be taken with dogs that have access to the street.

scholarly journals A rapid point-of-care assay accurately measures vitamin D

2021 ◽  
Author(s):  
K. Albrecht ◽  
J. Lotz ◽  
L. Frommer ◽  
K. J. Lackner ◽  
G. J. Kahaly
Keyword(s):  
Vitamin D ◽  
Metabolic Pathways ◽  
Point Of Care ◽  
Immunofluorescence Assay ◽  
Laboratory Method ◽  
Controlled Study ◽  
Mean Deviation ◽  
Serum Samples ◽  
Assay Validation ◽  
Accuracy And Precision

Abstract Purpose Vitamin D (VitD) is a pleiotropic hormone with effects on a multitude of systems and metabolic pathways. Consequently, the relevance of a sufficiently high VitD serum level becomes self-evident. Methods A rapid immunofluorescence assay designed for the point-of-care measurement of serum VitD3 solely was tested. Inter- and intra-assay validation, double testing and result comparison with a standardized laboratory method were performed. Results An overall linear correlation of r = 0.89 (Pearson, 95% CI 0.88–0.92, p < 0.01) between the point of care and the conventional reference assay was registered. Accuracy and precision were of special interest at cut-points (10 ng/ml [mean deviation 1.7 ng/ml, SD 1.98 ng/ml, SE 0.16 ng/ml], 12 ng/ml [MD 0.41, SD 1.89, SE 0.19] and 30 ng/ml [MD − 1.11, SD 3.89, SE 0.35]). Only a slight deviation was detected between the two assays when using fresh (r = 0.91, 95% CI 0.86–0.94, p < 0.01) and frozen serum samples (r = 0.86, 0.82–0.89, p < 0.01). Results remained steady when samples were frozen several times. Inter- and intra-assay validation according to the CLSI protocol as well as multiuser testing showed stable results. Conclusion This novel, innovative, and controlled study indicates that the evaluated rapid point of care VitD assay is reliable, accurate, and suited for clinical practice.

scholarly journals Antibodies anti-trypanosomatides in domestic cats in Paraná: who is at highest risk of infection?

2018 ◽  
Vol 27 (2) ◽  
pp. 232-236 ◽  
Author(s):  
Andressa Maria Rorato Nascimento de Matos ◽  
Eloiza Teles Caldart ◽  
Fernanda Pinto Ferreira ◽  
Keila Clarine Monteiro ◽  
Marielen de Souza ◽  
...  
Keyword(s):  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Domestic Cats ◽  
Serum Samples ◽  
Animal Group ◽  
Animal Protection ◽  
Leishmania Spp ◽  
In Series ◽  
Different Populations ◽  
Simple Logistic

Abstract The aim of this study were to detect antibodies anti-Leishmania spp. and anti-Trypanosoma cruzi in two different populations of domestic cats (Felis catus domesticus) from North Paraná referred for surgical castration and to determine which characteristics of the animals studied may be associated with seropositivity. Serum samples from 679 cats were analyzed using enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT) in series. Associations between age, sex, race, year of care and animal group were verified using the simple logistic regression. Percentage of 8.5% (58/679) of cats were positive for Leishmania spp. and 7.6% (51/673) for T. cruzi by the tests ELISA and IFAT. Animals collected by non-governmental animal protection organizations presented more seropositivity for Leishmania spp. (p<0.0001). Results shown that Leishmania spp. and T. cruzi are present in domestic cats in the northern part of the state of Paraná, as well as, owners of non-governmental animal protection organizations may be more exposed to leishmaniasis when compared to other animal owners evaluated in the present study.

scholarly journals Early Antibody Responses to Experimental Mycobacterium bovis Infection of Cattle

2006 ◽  
Vol 13 (6) ◽  
pp. 648-654 ◽  
Author(s):  
W. R. Waters ◽  
M. V. Palmer ◽  
T. C. Thacker ◽  
J. P. Bannantine ◽  
H. M. Vordermeier ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Skin Testing ◽  
Rapid Test ◽  
Kinetic Studies ◽  
Immunoblot Analysis ◽  
Immunoglobulin M ◽  
Serum Samples ◽  
Mycobacterial Antigens ◽  
New Generation ◽  
And Control

ABSTRACT Bovine tuberculosis persists as a costly zoonotic disease in numerous countries despite extensive eradication and control efforts. Sequential serum samples obtained from Mycobacterium bovis-infected cattle were evaluated for seroreactivity to mycobacterial antigens. Animals received M. bovis by aerosol, intratonsil, intranasal, or intratracheal inoculation. Assays included the multiantigen print immunoassay for determination of antigen recognition patterns, immunoblot analysis for sensitive kinetic studies, and the VetTB STAT-PAK test, a novel, rapid test based on lateral-flow technology. Responses to MPB83 were detected for all M. bovis-infected animals regardless of the route or strain of M. bovis used for inoculation. Other less commonly recognized antigens included ESAT-6, CFP-10, and MPB70. Responses to MPB83 were detectable as early as 4 weeks after inoculation, were boosted upon injection of purified protein derivatives for skin testing, and persisted throughout the course of each of the four challenge studies. MPB83-specific immunoglobulin M (IgM) was detected prior to MPB83-specific IgG detection; however, early IgM responses rapidly waned, suggesting a benefit of tests that detect both IgM- and IgG-specific antibodies. The VetTB STAT-PAK test detected responses in sera from 60% (15/25) of the animals by 7 weeks after challenge and detected responses in 96% (24/25) of the animals by 18 weeks. These findings demonstrate the potential for new-generation antibody-based tests for the early detection of M. bovis infection in cattle.

scholarly journals Investigation of the rapid immunochromatographic test performance in the diagnosis of syphilis; comparison of four serological methods

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Huseyin Agah Terzi ◽  
Ozlem Aydemir ◽  
Engin Karakece ◽  
Huseyin Hatipoglu ◽  
Mehmet Olmez ◽  
...  
Keyword(s):  
Test Performance ◽  
Gold Standard ◽  
Treponema Pallidum ◽  
Rapid Test ◽  
Immunochromatographic Test ◽  
Rapid Plasma Reagin ◽  
Serum Samples ◽  
Hemagglutination Assay ◽  
Predictive Values ◽  
Sensitivity Specificity

AbstractObjectivesTo test the performance of the newly available rapid test for syphilis, we compared it with Treponema pallidum hemagglutination assay (TPHA). Additionally, we investigated the performance of rapid plasma reagin (RPR) and chemiluminescence microparticle immunoassays (CMIA) at our laboratory using TPHA as a gold standard.MethodsThe serum samples of 595 patients with the pre-diagnosis of syphilis were studied by four serological methods. The sensitivity, specificity, and predictive values of RPR, CMIA, and syphilis rapid test were assessed by utilizing TPHA as a gold standard for the diagnosis of syphilis.ResultsOf the patients, 6.2% (37/595) had positive RPR, 5.5% (33/595) had positive CMIA, 5.5% (33/595) had a positive rapid immunochromatographic method and 5% (30/595) had positive TPHA. When TPHA results were taken as the reference, the sensitivity of the rapid test for syphilis was 100%, the specificity was 99.5%, PPV was 90.9%, and NPV was 100.0%.ConclusionsIt was observed that the rapid test for syphilis used in the study was quite successful, its cost was appropriate, and the test was very fast and easy to apply. At the same time, the agreement between syphilis rapid test and TPHA was found to be excellent.

Reactivity of serum samples from patients with a flavivirus infection measured by immunofluorescence assay and ELISA

2002 ◽  
Vol 4 (12) ◽  
pp. 1209-1215 ◽  
Author(s):  
Penelopie Koraka ◽  
Herve Zeller ◽  
Matthias Niedrig ◽  
Albert D.M.E Osterhaus ◽  
Jan Groen
Keyword(s):  
Immunofluorescence Assay ◽  
Serum Samples ◽  
Flavivirus Infection

scholarly journals A case of human monocytic ehrlichiosis in Serbia

2014 ◽  
Vol 142 (1-2) ◽  
pp. 79-82 ◽  
Author(s):  
Bogdan Arsic ◽  
Ana Gligic ◽  
Elizabeta Ristanovic ◽  
Branislav Lako ◽  
Aleksandar Potkonjak ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Clinical Picture ◽  
Peripheral Blood ◽  
Immunofluorescence Assay ◽  
High Fever ◽  
Tick Bite ◽  
Indirect Immunofluorescence Assay ◽  
Ehrlichia Chaffeensis ◽  
Serum Samples ◽  
History Of

Introduction. Ehrlichiosis is a bacterial zoonosis transmitted by hematophagous arthropods - ticks. In humans, it occurs as monocytic, granulocytic, and ewingii ehrlichiosis. Pathological process is based on parasitic presence of Ehrlichia organisms within peripheral blood cells - monocytes and granulocytes. Case Outline. Fifty-two year old patient was admitted to hospital due to high fever of over 40?C that lasted two days, accompanied with chills, muscle aches, malaise, loss of appetite, headache, confusion, breathing difficulties, and mild dry cough. The history suggested tick bite that occurred seven days before the onset of disease. Doxycycline was introduced and administered for 14 days, causing the disease to subside. Indirect immunofluorescence assay was used to analyze three serum samples obtained from this patient for Ehrlichia chaffeensis antibodies, and peripheral blood smear was evaluated for the presence of Ehrlichia and Ehrlichia aggregation into morulae. Conclusion. Ehrlichiosis should be considered in each case where there is a history of tick bite together with the clinical picture (high fever, chills, muscle aches, headache, generalized weakness and malaise, and possible maculopapular rash). The presence of Ehrlichia chaffeensis antibodies was confirmed in a patient with the history of tick bite, appropriate clinical picture and indirect immunofluorescence assay. This confirmed the presence of human monocytotropic ehrlichiosis, a disease that is uncommonly identified in our country.

scholarly journals Seroprevalence of SARS-CoV-2 infection in a municipality in southern Brazil

2021 ◽  
Vol 10 (9) ◽  
pp. e30710917996
Author(s):  
Maria Joana Carvalho e Silva ◽  
Kassia Kramer ◽  
Yasmim Paula Cesco ◽  
Kendy Otak ◽  
Andréia Machado Cardoso ◽  
...  
Keyword(s):  
Rna Virus ◽  
Rapid Test ◽  
Epidemiological Studies ◽  
Population Based ◽  
Southern Brazil ◽  
Cross Sectional ◽  
Population Based Study ◽  
Rapid Spread ◽  
And Control ◽  
The City

SARS-CoV-2 is an RNA virus, responsible for causing an acute respiratory syndrome, which became known worldwide for its rapid spread and for causing the pandemic of a new disease: COVID-19. Thus, epidemiological studies have been developed in order to determine the extent of the problems and the magnitude of the disease. The present study aimed to evaluate the seroprevalence of SARS-CoV-2 infection and its relationship with socio-demographic and clinical-epidemiological factors in the city of Chapecó, Santa Catarina, Brazil. It was a quantitative, epidemiological, observational, cross-sectional, population-based study with 2179 participants. Socio-demographic, associated behavioral, aggravating and comorbid parameters were evaluated, relating them to positivity or not in the rapid test implemented. The rapid test identified 22.2% of the diagnosed seroprevalence of COVID-19 in the municipality in question. A higher incidence of cases was observed in people living with other people, in people who had contact with positive cases, and in symptomatic patients, corroborating with current studies. The results highlight the importance of clinical-epidemiological studies for the understanding and control of COVID-19.

Sign in / Sign up

Export Citation Format

Share Document