scholarly journals Molecular detection of Mycoplasma suis in extensive pig production systems in the State of Maranhão, northeast Brazil

2019 ◽  
Vol 28 (2) ◽  
pp. 306-309 ◽  
Author(s):  
Mickaellen Susanny dos Santos Martins ◽  
Lucas Diniz Silva ◽  
Leandro Macêdo Miranda ◽  
Cristian Alex Aquino Lima ◽  
Renan Bressianini do Amaral ◽  
...  
Keyword(s):  
Structural Changes ◽  
Molecular Detection ◽  
Production Systems ◽  
The State ◽  
Rrna Gene ◽  
Northeast Brazil ◽  
Blood Samples ◽  
Pig Production ◽  
Mycoplasma Suis ◽  
First Time

Abstract Mycoplasma suis is a bacterium that causes hemoplasmosis in pigs. This agent is capable of adhering to the surface of porcine erythrocytes, inducing structural changes on these cells. In Brazil, there are few reports about the disease, its causal agent, and the economic impact of this pathogen on pig production systems and farm sanitation. The present study aimed to investigate the occurrence of M. suis in extensive swine farms located in the counties of Itapecuru Mirim, Santa Rita and Rosario, State of Maranhão, northeast Brazil. For such purpose, 64 blood samples of pigs from these facilities were tested for M. suis using a 16S rRNA gene-based quantitative real-time PCR (qPCR); 82.3%, 65.2% and 25% of blood samples of swine from farms in the cities of Itapecuru Mirim, Santa Rita and Rosario were positive for M. suis by qPCR, respectively. This study shows, for the first time, that M. suis circulates in pig populations from the state of Maranhão, Northeast Brazil.

Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey

2016 ◽  
Vol 61 (1) ◽  
Author(s):  
Handan Cetinkaya ◽  
Damla Haktanir ◽  
Seckin Arun ◽  
Cem Vurusaner
Keyword(s):  
Molecular Detection ◽  
Fragment Length Polymorphism ◽  
Common Species ◽  
Mycoplasma Species ◽  
Pcr Assay ◽  
Blood Samples ◽  
Total Prevalence ◽  
Polymerase Chain ◽  
First Time ◽  
Candidatus Mycoplasma Turicensis

AbstractThe aim of this study was to investigate Mycoplasma spp. species in blood samples of the domestic cats from the province of Istanbul, Turkey. Three hundred eighty four blood samples of client-owned cats were used for the identification of Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm) and Candidatus Mycoplasma turicensis (CMt) by Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) assays. Out of 384 blood samples, 74 (19.3%) were positive for one of Mycoplasma species. The total prevalence of Mhf, CMhm and CMt infections was 9.9%, 17.7% and 0.8% respectively. The most common species was CMhm. Co-infections were mostly with Mhf/CMhm and the frequency was 8.1%. Two cats were infected with three species. The current study was the first molecular prevalence study of hemotropic mycoplasmas in Istanbul, reporting the presence of CMt for the first time in Turkey. Prevalence of feline mycoplasma was notably high in Istanbul and PCR assay could be preferred rather than the microscopic examination for the diagnosis.

scholarly journals Molecular detection of bovine hemotrophic mycoplasmas in Mexico

2020 ◽  
Vol 13 (52) ◽  
pp. 67-82
Author(s):  
Rosa Estela Quiroz Castañeda ◽  
Kytzya Mejía Aragón ◽  
Hugo Aguilar Diaz ◽  
Jesús Francisco Preciado de la Torre
Keyword(s):  
Molecular Detection ◽  
Detection Method ◽  
Animal Health ◽  
Clinical Signs ◽  
Health Condition ◽  
Duplex Pcr ◽  
Blood Samples ◽  
End Point ◽  
Mycoplasma Wenyonii ◽  
First Time

The presence of hemoplasmas Candidatus Mycoplasma haemobos and Mycoplasma wenyonii that infect bovine cattle has been reported during the last years. Hemoplasmas may affect animal health either alone or in coinfections with other microorganisms, resulting in anemia and other clinical signs. In Mexico, only Ca. M. haemobos has been detected in cattle; in this work, we report for the first time in our country the presence of M. wenyonii in animals from different geographical sources amd we detected both hemoplasmas by duplex PCR. Also, by single end-point PCR, we found Ca. M. haemobos and M. wenyonii in 96% and 96.29% of the blood samples, respectively. Both hemoplasmas were detected in 50% of the samples analyzed, which suggest that the duplex PCR developed in this work might improve if some modifications are performed. This molecular detection method will provide valuable information to know the health condition of national cattle to prevent pathogen dispersion.

scholarly journals Occurrence and distribution of the Ceratiomyxales (Myxomycetes) in northeastern Brazil

2008 ◽  
Vol 51 (5) ◽  
pp. 971-980 ◽  
Author(s):  
Laise de Holanda Cavalcanti ◽  
Andrea Carla Caldas Bezerra ◽  
Antônia Aurelice Aurélio Costa ◽  
Inaldo do Nascimento Ferreira ◽  
Maria de Fátima de Andrade Bezerra
Keyword(s):  
Geographical Distribution ◽  
The State ◽  
Northeastern Brazil ◽  
Northeast Brazil ◽  
Herbarium Collections ◽  
Species Descriptions ◽  
First Time

Order Ceratiomyxales, which belongs to subclass Ceratiomyxomycetidae, includes the exospore bearing Myxomycetes, solely comprising family Ceratiomyxaceae and genus Ceratiomyxa, with four species. Based on the literature, on herbarium collections, and on recent surveys carried out by the authors in different states and ecosystems, it was possible to determine the occurrence and distribution of C. fruticulosa (cosmopolitan),C. morchella and C. sphaerosperma (predominantly tropical or subtropical) in northeastern Brazil. Species descriptions and illustrations are presented, as well as a map of their geographical distribution in eight of the nine states of Northeast Brazil. This order is being recorded for the first time for the state of Sergipe. Ceratiomyxa morchella and C. sphaerosperma are being cited for the first time for the state of Paraíba.

scholarly journals Porcine hemothropic mycoplasmas infection associated with productive impact in intensive pig production

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Fernando Antônio Moreira Petri ◽  
Karina Sonalio ◽  
Henrique Meiroz de Souza Almeida ◽  
Maria Eugênia Silveira Ferraz ◽  
Gabriel Yuri Storino ◽  
...  
Keyword(s):  
16S Rrna ◽  
Daily Weight Gain ◽  
Economic Losses ◽  
Rrna Gene ◽  
Finishing Pigs ◽  
Blood Samples ◽  
Pig Production ◽  
Rdna Gene ◽  
Spearman Correlation Test ◽  
Pig Farm

Abstract Background So far, three porcine hemoplasmas (PH) have been identified, namely Mycoplasma suis, Mycoplasma parvum, and Mycoplasma haemosuis. The first one is the main agent associated with porcine hemoplasmosis, a possible cause of economic losses in pig production. Thus, this work aimed to detect and quantify PH 16S rRNA in finishing pigs and to associate its load estimate with average daily weight gain (ADWG). For this purpose, whole blood samples from 318 pigs were collected at an age of 75 days (d0) when the pigs entered the finishing phase and 105 days later (d105). To calculate ADWG, the animals were weighed at the abovementioned dates. Then, DNA from blood samples were submitted to a qPCR targeting the 16S rRNA gene for PH. Spearman correlation test was performed to investigate potential associations between ADWG and the quantification values. Lastly, the molecular characterization of PH was done by sequencing the 23S rDNA gene. Results Out of the 318 samples, 190 (59.74%) were positive on d0, and 304 (95.6%) were positive on d105. A significant correlation was observed (p < 0.05), albeit with a low coefficient value (0.18), when comparing ADWG with quantification values on d105. The phylogenetic analysis based on the 23S rDNA gene showed that four sequences were closely related to M. parvum, and one sequence was positioned in the M. suis cluster. Conclusion Two PH, M. suis and M. parvum, were detected in a Brazilian pig farm. Moreover, increasing occurrence through time was observed, which may have affected the productive performance of positive animals, mainly at the end of the finishing phase, when antimicrobials are removed.

Scaphopoda (Mollusca) from the Brazilian continental shelf and upper slope (13º to 21ºS) with descriptions of two new species of the genus Cadulus Philippi, 1844

Zootaxa ◽  
2006 ◽  
Vol 1267 (1) ◽  
pp. 1 ◽  
Author(s):  
CARLOS HENRIQUE SOARES CAETANO ◽  
VICTOR SCARABINO ◽  
RICARDO SILVA ABSALÃO
Keyword(s):  
New Species ◽  
Continental Shelf ◽  
Geographical Distribution ◽  
The State ◽  
The Other ◽  
Geographical Range ◽  
Northeast Brazil ◽  
Two New Species ◽  
First Time ◽  
Upper Slope

Twenty six species of Scaphopoda, included in 12 genera and five families were identified. Within these, Fustiaria stenoschiza (Pilsbry & Sharp, 1897), Gadila pocula n.comb. (Dall, 1889); G. simpsoni n.comb. (Henderson, 1920) and G. pandionis (Verril & Smith in Verril, 1880) are recorded for the first time for Brazil and two new species of the genus Cadulus are here described. Besides that, six species previously recorded from north and northeast Brazil have their geographical range expanded southward: Antalis circumcincta (Watson, 1879), Episiphon didymum (Watson, 1879), Ep. sowerbyi (Guilding, 1834), Entalina platamodes (Watson, 1879), Gadila dominguensis (Orbigny, 1853) and Compressidens pressum (Pilsbry & Sharp, 1897). In the other hand, Paradentalium infractum n.comb. (Odhner, 1931) has its geographical distribution extended northward to the state of Maranhão (02ºS). Gadila acus (Dall, 1889) is proposed as junior synonym of G. dominguensis (Orbigny, 1853).

scholarly journals Characterization and typology of sheep and goat production systems in the State of Paraíba, a semi-arid region of northeastern Brazil

2017 ◽  
Vol 38 (4) ◽  
pp. 2163 ◽  
Author(s):  
Ricardo De Figueiredo Guilherme ◽  
Ana Milena César Lima ◽  
José Romero Alexandre Alves ◽  
Diego Figueiredo da Costa ◽  
Raymundo Rizaldo Pinheiro ◽  
...  
Keyword(s):  
Technology Use ◽  
Technical Assistance ◽  
Production Systems ◽  
Environmental Control ◽  
The State ◽  
Northeastern Brazil ◽  
Northeast Brazil ◽  
Breeding Activity ◽  
Epidemiological Surveys ◽  
Semi Arid

Breeding of goats and sheep is an important activity in northeast Brazil, contributing to economic and social development of this region. Thus, the objective of this study was to characterize goat and sheep rearing and breeding in the central-western region of the State of Paraíba, northeast Brazil. The study area included Borborema and mesoregions of the backlands of the state, represented by 127 municipalities in an area of 38.293 km2, with density of goats and sheep at 12.9 and 8.27 head/km2, respectively. A total of 62 farms belonging to five municipalities of the Borborema mesoregion and four municipalities of the Sertão mesoregion were surveyed. Epidemiological surveys were conducted in the farms, and questionnaires sought information on the economic, productive, and social aspects of the farm owners and the farms. The results allowed the classification of goat and sheep production systems in the semi-arid Paraíba as a family system and of subsistence, for farmers’ own consumption and for local trade, with low technology use and herds with up to 100 animals. The farmers’ level of education was low, and the investments and technical assistance for the development of breeding activity in the region were insufficient and/or inadequate. Thus, it was essential to conduct an economic study on the farms to assess the actual economic and financial status of breeding activity and the subsequent returns. It is suggested that these aspects be taken into account when planning livestock development policies, especially in terms of finance and technical assistance, as well as in the implementation of strategies to control commonly occurring diseases in the region, aimed at combating agents, environmental control, and protection of susceptible livestock.

scholarly journals Molecular and serological detection of Ehrlichia canis in naturally exposed dogs in Iran: an analysis on associated risk factors

2014 ◽  
Vol 23 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Nadi Maazi ◽  
Abdolali Malmasi ◽  
Parviz Shayan ◽  
Seyed Mahdi Nassiri ◽  
Taghi Zahraei Salehi ◽  
...  
Keyword(s):  
Risk Factors ◽  
Rural Areas ◽  
Ehrlichia Canis ◽  
Rrna Gene ◽  
Igg Antibodies ◽  
Serological Detection ◽  
Blood Samples ◽  
Associated Risk Factors ◽  
First Time ◽  
The 16S Rrna Gene

The general aim of this study, which was conducted for the first time in Iran, was to evaluate the seroprevalence and geographical distribution of Ehrlichia canis in a dog population in Iran, followed by molecular confirmation using PCR and sequencing. Blood samples were collected from 240 dogs in different areas of Alborz and Tehran Provinces and initially analyzed using the immunofluorescent antibody (IFA) test to detect anti-Ehrlichia canis IgG antibodies. Subsequently, nested PCR was performed based on a fragment of the 16S rRNA gene of E. canis on serologically positive samples. The results showed that 40/240 dogs (16.6%) presented anti-Ehrlichia canis IgG antibodies and that nine of the blood samples from the 40 seropositive dogs (22.5%) contained E. canis DNA, which was confirmed by sequencing. The seroprevalence of E. canis tended to be higher in purebred, one to three-year-old male dogs living in the Plain zone, in rural areas; however, this difference was not statistically significant.

Three new species of Cancellariidae (Gastropoda: Neogastropoda) from northeast Brazil with first record of Gergovia for the Atlantic ocean

Zootaxa ◽  
2007 ◽  
Vol 1387 (1) ◽  
pp. 59-68
Author(s):  
JOSÉ C.N. DE BARROS ◽  
SILVIO F.B. DE LIMA
Keyword(s):  
Atlantic Ocean ◽  
First Record ◽  
The State ◽  
The Body ◽  
Northeast Brazil ◽  
Deep Waters ◽  
Fernando De Noronha ◽  
Three New Species ◽  
Body Whorl ◽  
First Time

Brocchinia verheckeni n. sp. and Brocchinia harasewychi n. sp. are described from several stations from 340 to 720m on the Continental Slope off Northeast Brazil. Brocchinia verheckeni occurred from the states of Ceará to Sergipe, and B. harasewychi was collected from the states of Pernambuco to Alagoas. Both species are considered endemic to these regions. This is the second record of the genus Brocchinia Jousseaume, 1887 in deep waters off Brazil. Brocchinia harasewychi has a protoconch with 1 ¼ whorls, which is more inflated in comparison to B. verheckeni, the body whorl has 3 spiral cords, the uppermost bordering the suture and the other 2 near mid-whorl. The body whorl of Brocchinia verheckeni has 3 strongly nodulose spiral cords and an outer lip with 5 to 6 internal denticles, while that of B. harasewychi has a smooth outer lip. The genus Gergovia Cossmann, 1899 is recorded for the first time in the Atlantic Ocean with the description of Gergovia petiti n. sp., known only from the oceanic region from Northeast Brazil, occurring from the state of Ceará to the state of Bahia, as well as in the Fernando de Noronha archipelago, probably living in the muddy substrate at depths of between 206 and 720 meters.

Incidence and Control of Classical Swine Fever, with Special Reference to the Situation in the EEC

1989 ◽  
Vol 18 (1) ◽  
pp. 12-17
Author(s):  
H. J. Bendixen
Keyword(s):  
Virus Infection ◽  
Special Reference ◽  
Marked Reduction ◽  
Structural Changes ◽  
Production Systems ◽  
Classical Swine Fever ◽  
Pig Production ◽  
Technical Basis ◽  
And Control ◽  
Control And Eradication

Classical swine fever (CSF) has for almost a century been a serious problem for European pig producers, but concerted efforts in the Member States of the EEC have resulted in a marked reduction of the prevalence of the disease. The EEC is determined to eliminate it from its territory. This article describes the behaviour of the virus infection in pigs and the technical basis for control and eradication. Expansion and intensified production systems in the pig sector have made it difficult to control CSF and the necessity for structural changes in pig production is urged.

scholarly journals The Neotropical Amanita crebresulcata Bas: new citation from Northeast Brazil

Hoehnea ◽  
2007 ◽  
Vol 34 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Felipe Wartchow ◽  
Leonor Costa Maia
Keyword(s):  
The State ◽  
Amazon Forest ◽  
Northeast Brazil ◽  
Time Out ◽  
First Time

Amanita crebresulcata Bas, a species originally described from the State of Amazonas is reported for the second time out of the Amazon Forest and for the first time in northeast Brazil. Description, discussion and drawings of the species are provided.

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