scholarly journals Maternal undernutrition alters triiodothyronine concentrations and pituitary response to GnRH in fetal sheep

2002 ◽  
Vol 173 (3) ◽  
pp. 449-455 ◽  
Author(s):  
MT Rae ◽  
SM Rhind ◽  
CE Kyle ◽  
DW Miller ◽  
AN Brooks
Keyword(s):  
Adult Life ◽  
Reproductive Function ◽  
Maternal Blood ◽  
Pituitary Function ◽  
Reproductive Capacity ◽  
Fetal Sheep ◽  
Treatment Groups ◽  
Maternal Undernutrition ◽  
Igf I

The aims of this study were to determine which hormones may have a role in the expression of maternal undernutrition effects on reproductive function, in both the developing fetus and the adult offspring. This was undertaken by measuring the effects of long-term maternal undernutrition on metabolic hormone profiles and pituitary responses to single doses of GnRH and GH-releasing factor (GRF) in fetal sheep. From mating, groups of ewes were fed rations providing either 100% (HIGH) or 50% (LOW) of estimated metabolisable energy requirements for pregnancy throughout the experiment until slaughter at approximately 119 days of gestation. Fetal and maternal blood samples were collected from 113 until 119 days of gestation, via carotid and jugular catheters respectively, and assayed for insulin, IGF-I, GH, thyroxine and triiodothyronine (T(3)). Undernutrition had no effects on fetal weight, fetal gonad weight of either sex, fetal insulin or IGF-I concentrations. Male LOW fetuses exhibited a significantly attenuated response (P<0.05) to a bolus challenge of GnRH compared with HIGH fetuses. Basal fetal GH concentrations and the response to exogenous GRF were similar in both treatment groups, although LOW fetuses exhibited more secretory episodes (P<0.01). Mean T(3) concentrations were significantly lower in both the maternal (P<0.01) and fetal (P<0.05) plasma of LOW animals compared with HIGH animals. It is concluded that pituitary function was altered in fetal males and could influence male reproductive development. On the other hand, in female sheep, fetal gonadal abnormalities and reductions in reproductive capacity in adult life which are associated with fetal undernutrition are unlikely to be attributable to altered pituitary function. Additionally, these studies raise the possibility that thyroid hormones may have a role in the expression of maternal undernutrition effects on fetal development.

Pituitary and gonadal responses to the long-term pulsatile administration of gonadotrophin-releasing hormone in fetal fetal sheep

1997 ◽  
Vol 153 (3) ◽  
pp. 385-391 ◽  
Author(s):  
G B Thomas ◽  
A N Brooks
Keyword(s):  
Plasma Concentrations ◽  
Reproductive Function ◽  
Inverse Correlation ◽  
Experimental Period ◽  
Immediate Release ◽  
Late Gestation ◽  
Plasma Testosterone ◽  
Fetal Sheep ◽  
Pulsatile Administration

Abstract The fetal hypothalamo–pituitary–gonadal axis reaches a peak in activity at mid-gestation and this is followed by a period of suppression which persists until the onset of puberty. The decline in gonadotrophic activity during late gestation is thought to reflect the maturation of central and peripheral feedback signals. In order to establish if sustained pituitary responsiveness is rate limiting to the reinstatement of reproductive function, we have examined the endocrine consequences of repeated pulsatile GnRH administration to male and fetal sheep during late gestation. Beginning on day 121 of gestation (term=145 days) chronically catheterized fetal sheep were given i.v. pulses of either 500 ng GnRH or saline every 2 h for 14 days. Pituitary and gonadal responses were assessed by measuring changes in plasma concentrations of LH, FSH, inhibin and testosterone (in male fetuses) in response to the first pulse of GnRH on day 1 and to the corresponding pulse on days 4, 7, 10 and 14. In response to the first pulse of GnRH there was an immediate release of LH, with the peak response being significantly (P<0·01) greater than on subsequent days. In male fetuses each pulse of LH was followed by a rise in plasma testosterone concentrations within 40–60 min. The amplitude of these testosterone responses increased significantly (P<0·01) after 9 days of treatment despite a decline in the plasma LH response. Basal FSH concentrations increased progressively (P<0·05) during pituitary stimulation with GnRH in both male and female fetuses. Immunoreactive inhibin concentrations were significantly (P<0·05) higher in males than in females, and there was a gradual increase throughout the experimental period irrespective of treatment. We observed no inverse correlation between inhibin and FSH concentrations. These data show that pulsatile administration of GnRH to fetal sheep during late gestation results in sustained re-activation of pituitary–gonadal function. The decline in fetal gonadotrophins, which is a characteristic feature of late gestation, is therefore likely to result from inadequate GnRH secretion from the fetal hypothalamus rather than an inhibition of pituitary function by peripheral feedback signals. Journal of Endocrinology (1997) 153, 385–391

scholarly journals Pengaruh Daun Tapak Kambing (Ipomoae pes-caprae) Terhadap Konsentrasi Spermatozoa Tikus Putih (Rattus norvegicus)

2019 ◽  
Vol 11 (2) ◽  
pp. 95
Author(s):  
Kaspul Kaspul
Keyword(s):  
Randomized Trial ◽  
Rattus Norvegicus ◽  
Trial Design ◽  
Reproductive Function ◽  
Reproductive Capacity ◽  
Antioxidant Compounds ◽  
Reproductive Disorders ◽  
Treatment Groups ◽  
White Rats ◽  
All Treatment

Reproductive continuity is an important problem for humans and animals, but sometimes reproductive disorders occur which results in decreased reproductive capacity. Goat’s foot creeper leaves have active compounds that act as antioxidants. Antioxidant compounds can maintain reproductive function. This study aims to determine the effect of Goat’s foot creeper leaves on the spermatozoa concentration of white rats. Twenty white rats male (4-week-old) were treated with a completely randomized trial design. There were 4 treatment groups with 5 replications: (1) no treatment; (2) treatment with 750 mg goat’s foot creeper infusion per day; (3) treatment with 1500 mggfoot creeper leaves per day. All treatment groups were administered for 30 days orally. The results showed an increase in spermatozoa concentration of white rats.

Influence of the age at the first insemination and live weight on milk productivity of cows

2020 ◽  
Vol 50 (2) ◽  
pp. 57-63
Author(s):  
L. L. Petrukhina ◽  
S. L. Belozertseva
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Live Weight ◽  
Reproductive Function ◽  
Reproductive Capacity ◽  
Milk Productivity ◽  
Black And White ◽  
Irkutsk Region ◽  
White Breed

The materials of the long-term (2008–2018) research into the reproductive function of cattle of the black-and-white breed of Baikal type in Irkutsk region are presented. The influence of the age at the first insemination and live weight on cows’ milk productivity, lifelong productivity and productive longevity was studied. The experiment was conducted on the basis of materials from the breeder-farm for breeding cattle of Baikal type on the groups of cows of different ages: up to 14–20 months and older. Cows inseminated at 16 months (7917 kg) had the best milk yield in the first lactation. The largest milk yield was obtained from inseminated cows with live weight of 401-410 kg. Milk production of these heifers was 7902 kg in the first lactation, 8792 kg – in the second and 8710 kg – in the third. It was revealed that age of cows and their live weight at the first successful insemination had a positive effect on the lifelong productivity and productive longevity of cows. Cows whose age at the first successful insemination was 15 or 16 months had a longer duration of productive use (productive life span of animals was 2561–2558 days, respectively). The highest lifelong productivity (34379–34585 kg) was obtained from the cows of this group. The economic efficiency of lifelong milk production was determined depending on the age of the first insemination. The highest lifelong milk productivity was obtained from cows, which were first inseminated at the age of 15–16 months. The largest revenue from sales of additional products was also received from the cows of this group (366,058–370,480 roubles per head). The results obtained allow to develop effective methods for increasing reproductive capacity of cattle.

scholarly journals Fetal programming of insulin-like growth factor (IGF)-I and IGF-binding protein-3: evidence for an altered response to undernutrition in late gestation following exposure to periconceptual undernutrition in the sheep

1998 ◽  
Vol 159 (3) ◽  
pp. 501-508 ◽  
Author(s):  
BW Gallaher ◽  
BH Breier ◽  
CL Keven ◽  
JE Harding ◽  
PD Gluckman
Keyword(s):  
Binding Protein ◽  
Late Gestation ◽  
Fetal Sheep ◽  
Fetal Plasma ◽  
Maternal Undernutrition ◽  
Igf I ◽  
Igf Binding ◽  
Ad Libitum ◽  
Igf Binding Protein ◽  
Igfbp 3

It has been demonstrated in several animal models that undernutrition in utero has significant long lasting effects on subsequent fetal and postnatal development. To address the hypothesis that the insulin-like growth factors (IGFs) may mediate such effects, our study examined whether a period of periconceptual maternal undernutrition could have a lasting influence on the IGF axis in the fetal sheep. Ewes were either allowed to feed ad libitum or kept undernourished from day 60 prior to mating until day 30 after conception, and then both groups were allowed to feed ad libitum. These groups were further divided at day 105 of gestation, either being fed ad libitum or undernourished until day 115 of gestation. Fetal and maternal blood samples were obtained at both day 105 and 115 of gestation. We describe the development of a specific homologous RIA to measure ovine IGF-binding protein-3 (IGFBP-3) in fetal and maternal sheep plasma. Fetal plasma IGFBP-3 and IGF-I concentrations were significantly (P<0.05) reduced at day 115 of gestation after maternal undernutrition. The fetal plasma IGFBP-2 levels were unchanged. The degree of reduction in fetal plasma IGFBP-3 and IGF-I between day 105 and 115 of gestation as a response to acute maternal undernutrition was significantly greater (P<0.05) in fetuses of mothers receiving low periconceptual nutrition. The response of maternal plasma IGFBP-3 and IGF-I to undernutrition did not depend on the level of periconceptual nutrition. Western blot data indicate that changes in either maternal or fetal plasma IGFBP-3 concentrations were not the result of increased proteolytic activity. These results suggest that exposure to maternal periconceptual undernutrition reprograms IGFBP-3 and IGF-I regulation in the developing sheep fetus, altering its response to undernutrition in late gestation.

scholarly journals Dietary carotenoids affect the development of individual differences and behavioral plasticity

2019 ◽  
Vol 30 (5) ◽  
pp. 1273-1282 ◽  
Author(s):  
Shannon R Kelleher ◽  
Aimee J Silla ◽  
Petri T Niemelä ◽  
Niels J Dingemanse ◽  
Phillip G Byrne
Keyword(s):  
Individual Variation ◽  
Behavioral Plasticity ◽  
Life Stage ◽  
Adult Life ◽  
Life Stages ◽  
Treatment Groups ◽  
Nutritional Conditions ◽  
Exploration Behavior ◽  
Irreversible Effects

AbstractNutritional conditions experienced during development are expected to play a key role in shaping an individual’s behavioral phenotype. The long term, irreversible effects of nutritional conditions on behavioral variation among and within individuals remains largely unexplored. This study aimed to investigate how long-term carotenoid availability (representing low vs. high quality nutritional conditions) during both larval and adult life stages influences the expression of among-individual variation (animal personality) and within-individual variation (behavioral plasticity). We tested for personality and plasticity along the exploration/avoidance behavioral axis in the Southern Corroboree frog (Pseudophryne corroboree). We predicted that treatment groups receiving carotenoids during early development would be more exploratory and have greater among- and within-individual variation compared with individuals that did not receive carotenoids (i.e., silver spoon hypothesis). Superior nutritional conditions experienced during development are expected to provide individuals with resources needed to develop costly behaviors, giving them an advantage later in life irrespective of prevailing conditions. Unexpectedly, frogs that did not receive carotenoids as larvae expressed greater among-individual variance in exploration behavior. Additionally, frogs that did not receive carotenoids at either life stage displayed greater within-individual variance. Our findings provide no support for the silver spoon hypothesis but suggest that inconsistent nutritional conditions between life stages may adversely affect the development of behavioral phenotypes. Overall, our results indicate that early and late life nutritional conditions affect the development of personality and plasticity. They also highlight that nutritional effects on behavior may be more complex than previously theorized.

The lack of impact of peri-implantation or late gestation nutrient restriction on ovine fetal renal development and function

2011 ◽  
Vol 2 (4) ◽  
pp. 236-249 ◽  
Author(s):  
L. M. Braddick ◽  
D. M. Burrage ◽  
J. K. Cleal ◽  
D. E. Noakes ◽  
M. A. Hanson ◽  
...  
Keyword(s):  
Kidney Development ◽  
Adult Life ◽  
Late Gestation ◽  
Fetal Life ◽  
Ang Ii ◽  
Nutrient Requirements ◽  
Fetal Sheep ◽  
Fetal Biometry ◽  
Maternal Undernutrition ◽  
And Function

Unbalanced nutrition during critical windows of development is implicated in determining the susceptibility to hypertension and cardiovascular disease in adult life, but the underlying mechanisms during fetal life have not been fully elucidated. We investigated the effects of moderate nutritional restriction during critical windows in gestation on late gestation fetal sheep growth, cardiovascular and renal renin-angiotensin system function. Ewes were fed 100% nutrient requirements (control), or 40–50% nutrient requirements during the peri-implantation period (1–31 days gestation (dGA), PI40 and PI50), or 50% nutrient requirements in late gestation (104–127 dGA). At 125 ± 2 dGA, fetal cardiovascular and renal function were measured at baseline, and during frusemide, angiotensin II (Ang II), phenylephrine and hypoxia challenges. Maternal undernutrition had no effect on fetal biometry, kidney weight, nephron number, basal cardiovascular function or cardiovascular and renal responses to frusemide. Fetal blood pressure response to Ang II was blunted in PI50 (P< 0.05), but not in PI40 groups. There was no difference between groups in the cardiovascular or endocrine response to hypoxia. The lack of effect of moderate undernutrition within key developmental windows of fetal kidney development on fetal renal structure and function suggests that renal mechanisms do not underlie our previous observations of cardiovascular dysfunction in adulthood following early-life undernutrition.

scholarly journals Rituximab-Containing Risk-Adapted Treatment Strategy in Nodular Lymphocyte Predominant Hodgkin Lymphoma: 7-Years Follow-Up

Cancers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1760
Author(s):  
Novella Pugliese ◽  
Marco Picardi ◽  
Roberta Della Pepa ◽  
Claudia Giordano ◽  
Francesco Muriano ◽  
...  
Keyword(s):  
Side Effects ◽  
Hodgkin Lymphoma ◽  
Prognostic Score ◽  
Single Agent ◽  
Response To Therapy ◽  
Baseline Risk ◽  
Historical Cohort ◽  
Treatment Groups

Background: Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) is a rare variant of HL that accounts for 5% of all HL cases. The expression of CD20 on neoplastic lymphocytes provides a suitable target for novel treatments based on Rituximab. Due to its rarity, consolidated and widely accepted treatment guidelines are still lacking for this disease. Methods: Between 1 December 2007 and 28 February 2018, sixteen consecutive newly diagnosed adult patients with NLPHL received Rituximab (induction ± maintenance)-based therapy, according to the baseline risk of German Hodgkin Study Group prognostic score system. The treatment efficacy and safety of the Rituximab-group were compared to those of a historical cohort of 12 patients with NLPHL who received Doxorubicin, Bleomycin, Vinblastine, Dacarbazine (ABVD) chemotherapy followed by radiotherapy (RT), if needed, according to a similar baseline risk. The primary outcome was progression-free survival (PFS) and secondary outcomes were overall survival (OS) and side-effects (according to the Common Terminology Criteria for Adverse Events, v4.03). Results: After a 7-year follow-up (range, 1–11 years), PFS was 100% for patients treated with the Rituximab-containing regimen versus 66% for patients of the historical cohort (p = 0.036). Four patients in the latter group showed insufficient response to therapy. The PFS for early favorable and early unfavorable NLPHLs was similar between treatment groups, while a better PFS was recorded for advanced-stages treated with the Rituximab-containing regimen. The OS was similar for the two treatment groups. Short- and long-term side-effects were more frequently observed in the historical cohort. Grade ≥3 neutropenia was more frequent in the historical cohort compared with the Rituximab-group (58.3% vs. 18.7%, respectively; p = 0.03). Long-term non-hematological toxicities were observed more frequently in the historical cohort. Conclusion: Our results confirm the value of Rituximab in NLPHL therapy and show that Rituximab (single-agent) induction and maintenance in a limited-stage, or Rituximab with ABVD only in the presence of risk factors, give excellent results while sparing cytotoxic agent- and/or RT-related damage. Furthermore, Rituximab inclusion in advanced-stage therapeutic strategy seems to improve PFS compared to conventional chemo-radiotherapy.

scholarly journals The long-term bio-behavioural effects of juvenile sildenafil treatment in Sprague-Dawley versus Flinders Sensitive Line rats

2021 ◽  
pp. 1-20
Author(s):  
Juandré Lambertus Bernardus Saayman ◽  
Stephanus Frederik Steyn ◽  
Christiaan Beyers Brink
Keyword(s):  
Sprague Dawley ◽  
Long Term Effects ◽  
Bdnf Level ◽  
Treatment Groups ◽  
Sd Rats ◽  
Behavioural Effects ◽  
Sensitive Line ◽  
Level Analysis ◽  
Flinders Sensitive Line

Abstract Objective: To investigate the long-term effects of juvenile sub-chronic sildenafil (SIL) treatment on the depressive-like behaviour and hippocampal brain-derived neurotrophic factor (BDNF) levels of adult Sprague-Dawley (SD) versus Flinders Sensitive Line (FSL) rats. Methods: SD and FSL rats were divided into pre-pubertal and pubertal groups, whereafter 14-day saline or SIL treatment was initiated. Pre-pubertal and pubertal rats were treated from postnatal day 21 (PND21) and PND35, respectively. The open field and forced swim tests (FST) were performed on PND60, followed by hippocampal BDNF level analysis one day later. Results: FSL rats displayed greater immobility in the FST compared to SD rats (p < 0.0001), which was reduced by SIL (p < 0.0001), regardless of treatment period. Hippocampal BDNF levels were unaltered by SIL in all treatment groups (p > 0.05). Conclusion: Juvenile sub-chronic SIL treatment reduces the risk of depressive-like behaviour manifesting during young adulthood in genetically susceptible rats.

scholarly journals CD34+ human marrow cells that express low levels of Kit protein are enriched for long-term marrow-engrafting cells

Blood ◽  
1996 ◽  
Vol 87 (10) ◽  
pp. 4136-4142 ◽  
Author(s):  
I Kawashima ◽  
ED Zanjani ◽  
G Almaida-Porada ◽  
AW Flake ◽  
H Zeng ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
In Utero ◽  
Fetal Sheep ◽  
Hematopoietic Stem ◽  
Show Evidence ◽  
Marrow Cells

Using in utero transplantation into fetal sheep, we examined the capability of human bone marrow CD34+ cells fractionated based on Kit protein expression to provide long-term in vivo engraftment. Twelve hundred to 5,000 CD34+ Kit-, CD34+ Kit(low), and CD34+ Kit(high) cells were injected into a total of 14 preimmune fetal sheep recipients using the amniotic bubble technique. Six fetuses were killed in utero 1.5 months after bone marrow cell transplantation. Two fetuses receiving CD34+ Kit(low) cells showed signs of engraftment according to analysis of CD45+ cells in their bone marrow cells and karyotype studies of the colonies grown in methylcellulose culture. In contrast, two fetuses receiving CD34+ Kit(high) cells and two fetuses receiving CD34+ Kit- cells failed to show evidence of significant engraftment. Two fetuses were absorbed. A total of six fetuses receiving different cell populations were allowed to proceed to term, and the newborn sheep were serially examined for the presence of chimerism. Again, only the two sheep receiving CD34+ Kit(low) cells exhibited signs of engraftment upon serial examination. Earlier in studies of murine hematopoiesis, we have shown stage-specific changes in Kit expression by the progenitors. The studies of human cells reported here are in agreement with observations in mice, and indicate that human hematopoietic stem cells are enriched in the Kit(low) population.

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