scholarly journals Effect of Lepidium meyenii (Maca) on spermatogenesis in male rats acutely exposed to high altitude (4340 m)

2004 ◽  
Vol 180 (1) ◽  
pp. 87-95 ◽  
Author(s):  
GF Gonzales ◽  
M Gasco ◽  
A Cordova ◽  
A Chung ◽  
J Rubio ◽  
...  
Keyword(s):  
High Altitude ◽  
Sea Level ◽  
Sperm Count ◽  
Treated Group ◽  
Stage I ◽  
Male Rats ◽  
Dependent Manner ◽  
Epididymal Sperm ◽  
Lepidium Meyenii ◽  
Stage Viii

Lepidium meyenii (Maca) is a Peruvian hypocotyl that grows exclusively between 4000 and 4500 m in the central Andes. Maca is traditionally employed in the Andean region for its supposed fertility-enhancing properties.The aim of this study was to test the hypothesis that Maca can prevent high altitude-induced testicular disturbances. Adult male rats were exposed for 21 days to an altitude of 4340 m and treated with vehicle or aqueous extract of Maca (666.6 mg/day). The lengths of the stages of the seminiferous epithelium and epididymal sperm counts were obtained at 0, 7, 14 and 21 days of exposure. The stages of the seminiferous tubules were assessed by transillumination. A dose-response study was also performed at sea level to determine the effect of Maca given to male rats at doses of 0, 6.6, 66.6 and 666.6 mg/day for 7 days on body weight, seminiferous tubule stages and epididymal sperm count. The length of stage VIII and the epididymal sperm count were increased in a dose-dependent manner in Maca-treated rats but treatment reduced the length of stage I. At the highest dose, sperm count increased 1.58 times, the length of stage VIII increased 2.4 times and the length of stage I was reduced 0.48 times compared with the value at dose 0. Exposure to high altitude resulted in a reduction in epididymal sperm count after 7 days and lower values were maintained up to 21 days. Altitude reduced spermiation (stage VIII) to half and the onset of spermatogenesis (stages IX-XI) to a quarter on days 7 and 14 but treatment with Maca (666.6 mg/day) prevented these changes. Data on transillumination and epididymal sperm count in the Maca-treated group exposed to high altitude were similar to those obtained at sea level. Maca increased the sperm count on day 21 of exposure to high altitude to values similar (1095.25 +/- 20.41x10(6) sperm, means +/- S.E.M.) to those obtained in the Maca-treated group at sea level (1132.30 +/- 172.95x10(6) sperm). Furthermore, in the Maca-treated group exposed for 21 days to high altitude, epididymal sperm count was higher than in the non-treated group at sea level (690.49 +/- 43.67x10(6) sperm). In conclusion, treatment of rats with Maca at high altitude prevented high altitude-induced spermatogenic disruption.

Effect of high altitude exposure on spermatogenesis and epididymal sperm count in male rats

Andrologia ◽  
2003 ◽  
Vol 35 (6) ◽  
pp. 368-374
Author(s):  
M. Gasco ◽  
J. Rubio ◽  
A. Chung ◽  
L. Villegas ◽  
G. F. Gonzales
Keyword(s):  
High Altitude ◽  
Sperm Count ◽  
Male Rats ◽  
Epididymal Sperm ◽  
Altitude Exposure

Effect of high altitude exposure on spermatogenesis and epididymal sperm count in male rats

Andrologia ◽  
2003 ◽  
Vol 35 (6) ◽  
pp. 368-374 ◽  
Author(s):  
M. Gasco ◽  
J. Rubio ◽  
A. Chung ◽  
L. Villegas ◽  
G. F. Gonzales
Keyword(s):  
High Altitude ◽  
Sperm Count ◽  
Male Rats ◽  
Epididymal Sperm ◽  
Altitude Exposure

scholarly journals Effect of Tramadol on Sperm Profile of Male Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
I. S. Esua ◽  
U. U. Uno ◽  
U. B. Ekaluo
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Head ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Sperm Viability ◽  
Albino Rat ◽  
Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

Spermatotoxic effects of carbaryl in rats

1996 ◽  
Vol 15 (9) ◽  
pp. 736-738 ◽  
Author(s):  
N. Pant ◽  
R. Shankar ◽  
SP Srivastava
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Young Male ◽  
Male Rats ◽  
Epididymal Sperm ◽  
Abnormal Morphology ◽  
Age Dependent ◽  
Effect Level

The spermatotoxic effect of carbaryl in adult and young male rats has been examined. Carbaryl 50 and 100 mg/kg b.wt. Male fed 5 d/week for 60 days, caused dose and age- dependent decline in epididymal sperm count and sperm motility, an increase in sperm with abnormal morphology. The dose of 25 mg/kg/d was a 'No observed effect level' for the indices studied. Young animals in comparison to adults exhibited pronounced spermatotoxic effects.

scholarly journals Effect of Thymus vulgaris leaf extract on cadmium-induced testicular toxicity in rats

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Remigius Ibe Onoja ◽  
Chinwe Uzoma Chukwudi ◽  
Emmanuel Uchechukwu Ugwueze ◽  
Davinson Chuka Anyogu ◽  
Wilson Obidah ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Sperm Count ◽  
Treated Group ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Thymus Vulgaris ◽  
Testicular Toxicity ◽  
Histological Changes ◽  
Ameliorative Effect ◽  
Testicular Injury

Abstract Background Cadmium (Cd) is a known metallohormone which mimics the action of steroid hormones with adverse effect on testicular function. It is highly toxic and a prevalent environmental contaminant with no conventional antidote. This study investigates the possible ameliorative effects of Thymus vulgaris extract on testicular toxicity induced by Cd in male rats. Results The testicular and epididymal weights, serum concentration of follicle stimulating hormone, luteinizing hormone, and testosterone were significantly (p ≤ 0.05) lower in the cadmium-treated group compared to the control. Necrosis of germ cells of the seminiferous tubules was observed in the testicular tissues of the cadmium-treated group. Administration of extract showed mild but non-significant (p ≥ 0.05) protective effect on the cadmium-induced decrease in sex hormones and sperm count as well as oxidative stress and histological changes. Conclusion Thymus vulgaris leaf extract had weak ameliorative effect on cadmium-induced testicular injury in rats but with promising antioxidant activity.

423. Effects of Eurycoma longifolia Jack on spermatogenesis and sperm counts of male rats treated with oestradiol

2008 ◽  
Vol 20 (9) ◽  
pp. 103
Author(s):  
A. Norhazlina ◽  
A. Wan Nurul Heriza ◽  
M. Norfilza ◽  
P. Moratv
Keyword(s):  
Cell Count ◽  
Sperm Count ◽  
Treated Group ◽  
Spermatogenic Cell ◽  
Male Rats ◽  
Control Group ◽  
Root Extract ◽  
Healthy Male ◽  
Eurycoma Longifolia ◽  
Sperm Counts

Eurycoma longifolia has been known for its aphrodisiac effects in male. Our previous study showed that with the dosage of 8 mg/kg bodyweight (BW) Eurycoma longifolia root extract increased plasma total testosterone levels in male rats when given for 14 days. Oestrogen is a potent inhibitor for testosterone production and spermatogenesis. The aim of the present study is to determine the effects of Eurycoma longifolia (8 mg/kg BW) on the spermatogenic cell count and sperm count of testosterone-suppressed male rats. Adult male Sprague-Dawley healthy male rats weighed 200–250 g were treated with, either control vehicle (no active ingredients given), oestradiol (500 mg/kg BW), Eurycoma longifolia (8 mg/kg BW) or combination of Eurycoma longifolia and oestradiol for fourteen consecutive days. Results showed that sperm count and spermatogenic cell count were increased in Eurycoma longifolia treated group compared with control group (P < 0.05) and to oestradiol treated group (P < 0.05). While in oestradiol treated group, sperm count and spermatogenic cell count were reduced significantly (P < 0.05) compared with control group. Combination of Eurycoma longifolia and oestradiol did not affect sperm count and spermatogenic cell count when compared with control group but they were significantly increased compared with oestradiol treated group (P < 0.05). Thus, the study has shown that Eurycoma longifolia is potentially capable to suppress harmful effects of oestradiol on spermatogenesis and sperm counts in healthy male rats when given for fourteen consecutive days.

Red Maca (Lepidium meyenii), a Plant from the Peruvian Highlands, Promotes Skin Wound Healing at Sea Level and at High Altitude in Adult Male Mice

2017 ◽  
Vol 18 (4) ◽  
pp. 372-383 ◽  
Author(s):  
Denisse Nuñez ◽  
Paola Olavegoya ◽  
Gustavo F. Gonzales ◽  
Cynthia Gonzales-Castañeda
Keyword(s):  
Wound Healing ◽  
High Altitude ◽  
Sea Level ◽  
Adult Male ◽  
Skin Wound ◽  
Male Mice ◽  
Skin Wound Healing ◽  
Lepidium Meyenii

scholarly journals Effects of Prolonged Treatment With Co-trimoxazole on the Thyroid Gland, Liver, and Epididymal Sperm Reserve in Dogs

2017 ◽  
Vol 10 ◽  
pp. 117955571774288 ◽  
Author(s):  
Davinson C Anyogu ◽  
Vincent SO Shoyinka ◽  
John I Ihedioha
Keyword(s):  
Thyroid Gland ◽  
Sperm Count ◽  
Treated Group ◽  
Prolonged Treatment ◽  
Epididymal Sperm ◽  
Follicular Cells ◽  
Standard Procedures ◽  
New Findings ◽  
Protozoan Infections ◽  
Dose Dependent

Co-trimoxazole is an antimicrobial drug gotten from potentiation of sulfamethoxazole with trimethoprim. It is widely used for the treatment of bacterial and protozoan infections in humans. It is also used in veterinary clinics against susceptible microorganisms, but thyroid dysfunction has raised concern especially in dogs. This study aimed to determine the effects of prolonged treatment with co-trimoxazole in euthyroid dogs. Dogs were given co-trimoxazole at 30, 60, and 120 mg/kg body weight at 12-hour intervals for 21 days. Standard procedures were used to assay total T4 and T3, thyrotropin, testosterone, aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in serum. The thyroid gland and testes were weighed. In addition, thyroid and liver were examined histologically. Epididymal sperm count was also performed. Co-trimoxazole caused dose-dependent depression of serum thyroxine levels with severe colloid depletion, intrafollicular hemorrhage, hyperplasia, and hypertrophy of the follicular cells. The liver showed vacuolar hepatopathy. Epididymal sperm count was low in the 120 mg/kg–treated group. The study revealed that thyroid hemorrhage and lowered epididymal sperm reserve were new findings in co-trimoxazole toxicity in dogs.

scholarly journals Effect of Glibenclamide on Motor and Behavioral Activity of Animals with Traumatic Brain Injury in the Highlands

2021 ◽  
Vol 7 (6) ◽  
Author(s):  
M. Shuvalova ◽  
Yu. Shidakov ◽  
A. Shanazarov
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Locomotor Activity ◽  
High Altitude ◽  
Sea Level ◽  
Brain Injuries ◽  
White Male ◽  
Statistical Processing ◽  
Male Rats ◽  
Behavioral Activity

Human activity is associated with the risk of injury. The rate of cases of traumatic brain injury (TBI) in high-altitude conditions is high. It should be assumed that exogenous hypoxia will have a significant impact on the development of the clinical consequences of TBI. However, information about the behavior of animals on the background of TBI in the highlands is scarce. The search for means of correcting brain injuries remains an urgent issue. To date, glibenclamide has been proposed for this purpose, but its effect in the highlands has not been studied. Objective: to evaluate the effect of glibenclamide on the behavioral activity of animals with TBI in the highlands. The object of the study is 82 white male rats weighing 250–310 g. The low-mountain series of the experiment was carried out at an altitude of 760 m above sea level (Bishkek). The high-altitude series was modeled on the Tuya-Ashu pass — 3200 m above sea level (Kyrgyzstan). TBI was reproduced according to the method of Y. Tang (1997). Correction with glibenclamide at a dose of 0.1 mg/kg per os. Behavioral activity was evaluated using the Open Field method, and muscle strength was evaluated using the S. V. Speransky method on the 3rd day of the experiment. Statistical processing of the obtained data was carried out in the SPSS 16.0 program. TBI in the low mountains resulted in a decrease in locomotor activity by 67% (P<0.001), and efficiency — by 43% (P<0.001). In the group of rats with TBI in the highlands, compared with the data of healthy animals that visited the same altitude, locomotor activity decreased by 44% (P<0.001), racks — by 60% (P<0.001), minks — by 76% (P< 0.01), grooming — by 55% (P<0.01), the number of boluses of defecation increases by 37% (P<0.05). Correction of TBI with glibenclamide in the highlands led to an increase in locomotion by 2 times (P<0.001), standing — by 2.3 times, peering into minks — by 4 times (P<0.01), working capacity — by 2.04 times (P<0.001). The level of defecation decreased by 70% (P<0.001). Violations of the behavior of rats in the highlands with TBI without the use of glibenclamide are more pronounced than in experiments in the foothills. Correction of TBI that occurred in the highlands demonstrates a positive neurotropic effect of glibenclamide.

scholarly journals Study the Physiological Effects of Finasteride (Prostacare) on some Reproductive Parameters in Male Rats (Rattus- Rattus)

2020 ◽  
Vol 13 (1) ◽  
pp. 91-96
Author(s):  
Assad AL-Edany
Keyword(s):  
Sperm Count ◽  
Rattus Rattus ◽  
Treated Group ◽  
Reproductive Hormones ◽  
Male Rats ◽  
Control Group ◽  
Physiological Effects ◽  
Reproductive Parameters ◽  
Live Sperm ◽  
Animal House

This study was conducted in the veterinary medicine college in basrah university animal house and aimed to investigate the effect of finasteride (prostacare) on malesome parameters reproductive system in albino male rats (Rattua-Rattus). Thirty six mature male rats with body weight 200-210 g and age 8 weeks were randomly divided into three groups (12 animal each group). The first group was considered as control group and treated orally normal salin (0.9%N.S)during the experimental 30 days , the second group was treated finasteride(0.016mg/kg of body weigt oraiiy during the experimental(30)days.and the third group was administrated finasteride(0.032mglkg of body weigt orally during the experimental 30 days. The results showed a significant decrease in the sperm count ,sperm motility ,live sperm and increase in dead sperm percentage as well as a significant decrease in the level of reproductive hormones (testosterone, LH and FSH) in treated group with finasteride compared with control group , from this study it was concluded that the finasteride has undesired physiological effects on fertility in albino male rats (Rattus-Rattus).

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