Nutrient mobilization, growth and field survival of Pinus pringlei inoculated with three ectomycorrhizal mushrooms

Background. All of the Pinaceae require the ectomycorrhizal interaction to survive under field conditions. However, their use has not traditionally been considered in the production of forest plants in Neotropical areas.Hypothesis. There will be an increase in plant quality in greenhouse and in survival in the field of Pinus pringlei inoculated with ectomycorrhizal fungi (ECMF).Site of study. The experiment was carried out in Texcoco, Mexico, in two sites: i) greenhouse of the Colegio de Postgraduados; and ii) in the field, in the San Pablo Ixayoc town.Methods. P. pringlei plants, a native species of Mexico, were inoculated with the ECMF: i) Hebeloma alpinum (Ha); ii) Laccaria trichodermophora (Lt); and iii) Thelephora terrestris (Tt). The effect of this inoculation on plant growth and nutritient translocation was evaluated. A morphological and molecular characterization of ectomycorrhizal roots and an evaluation of the seedling field survival were carried out, and compared with non-inoculated plants.Results. Pinus pringlei plants inoculated with ECMF increased their growth and nutrient content. A significant nutritional allocation from root to stem mainly of K, Fe, and Zn was recorded.The morpho-anatomical and molecular characterization showed the presence of Ha, Lt and Tt in ectomycorrhizal roots. Two-year old plants mainly inoculated with Ha and Tt showed higher survival in the field than non-inoculated plants.Conclusions. The beneficial effects of ECMF on P. pringlei growth, nutrimental translocation (mainly on K, Fe and Zn), and field survival rates are reported for the first time.

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Molecular Characterization of the Gene Encoding a New AmpC β-Lactamase in a Clinical Strain of Acinetobacter Genomic Species 3

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.48.4.1374-1378.2004 ◽

2004 ◽

Vol 48 (4) ◽

pp. 1374-1378 ◽

Cited By ~ 17

Author(s):

Alejandro Beceiro ◽

Lourdes Dominguez ◽

Anna Ribera ◽

Jordi Vila ◽

Francisca Molina ◽

...

Keyword(s):

Nucleotide Sequence ◽

Molecular Characterization ◽

Biochemical Properties ◽

Clinical Strain ◽

The Novel ◽

Gene Encoding ◽

Genomic Species ◽

First Time

ABSTRACT A presumptive chromosomal cephalosporinase (pI, 9.0) from a clinical strain of Acinetobacter genomic species 3 (AG3) is reported. The nucleotide sequence of this β-lactamase shows for the first time the gene encoding an AmpC enzyme in AG3. In addition, the biochemical properties of the novel AG3 AmpC β-lactamase are reported

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Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

Ciência Rural ◽

10.1590/0103-8478cr20141810 ◽

2015 ◽

Vol 45 (12) ◽

pp. 2197-2200 ◽

Cited By ~ 2

Author(s):

Thor Vinícius Martins Fajardo ◽

Monique Bezerra Nascimento ◽

Marcelo Eiras ◽

Osmar Nickel ◽

Gilvan Pio-Ribeiro

Keyword(s):

Amino Acid ◽

Molecular Characterization ◽

Molecular Analysis ◽

Nucleotide Sequences ◽

Amino Acid Sequences ◽

Causal Agent ◽

Ringspot Virus ◽

Prunus Necrotic Ringspot Virus ◽

First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

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Genome organization and molecular characterization of the three Formica exsecta viruses—FeV1, FeV2 and FeV4

PeerJ ◽

10.7717/peerj.6216 ◽

2019 ◽

Vol 6 ◽

pp. e6216 ◽

Cited By ~ 3

Author(s):

Kishor Dhaygude ◽

Helena Johansson ◽

Jonna Kulmuni ◽

Liselotte Sundström

Keyword(s):

Molecular Characterization ◽

Genome Organization ◽

Rna Virus ◽

Open Reading Frames ◽

Link Type ◽

Infection Pathways ◽

Comparative Genomics Analysis ◽

First Time ◽

Negative Sense

We present the genome organization and molecular characterization of the three Formica exsecta viruses, along with ORF predictions, and functional annotation of genes. The Formica exsecta virus-4 (FeV4; GenBank ID: MF287670) is a newly discovered negative-sense single-stranded RNA virus representing the first identified member of order Mononegavirales in ants, whereas the Formica exsecta virus-1 (FeV1; GenBank ID: KF500001), and the Formica exsecta virus-2 (FeV2; GenBank ID: KF500002) are positive single-stranded RNA viruses initially identified (but not characterized) in our earlier study. The new virus FeV4 was found by re-analyzing data from a study published earlier. The Formica exsecta virus-4 genome is 9,866 bp in size, with an overall G + C content of 44.92%, and containing five predicted open reading frames (ORFs). Our bioinformatics analysis indicates that gaps are absent and the ORFs are complete, which based on our comparative genomics analysis suggests that the genomes are complete. Following the characterization, we validate virus infection for FeV1, FeV2 and FeV4 for the first time in field-collected worker ants. Some colonies were infected by multiple viruses, and the viruses were observed to infect all castes, and multiple life stages of workers and queens. Finally, highly similar viruses were expressed in adult workers and queens of six other Formica species: F. fusca, F. pressilabris, F. pratensis, F. aquilonia, F. truncorum and F. cinerea. This research indicates that viruses can be shared between ant species, but further studies on viral transmission are needed to understand viral infection pathways.

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The Ornithodoros capensis group (Acari: Argasidae): a morphological diagnosis and molecular characterization of O. capensis sensu stricto from Queimada Grande Island, Brazil

Systematic and Applied Acarology ◽

10.11158/saa.22.1.5 ◽

2017 ◽

Vol 22 (1) ◽

pp. 28 ◽

Cited By ~ 4

Author(s):

Sebastián Muñoz-Leal ◽

Ricardo A. Dias ◽

Carlos R. Abrahão ◽

Marcelo B. Labruna

Keyword(s):

Molecular Characterization ◽

Related Species ◽

Morphological Analysis ◽

Bacterial Pathogens ◽

Sensu Stricto ◽

Closely Related Species ◽

Paulo State ◽

Morphological Diagnosis ◽

First Time

Ornithodoros capensis sensu lato (s. l.) is a worldwide-distributed group of soft ticks that parasitize birds in insular and continental lands. It is currently composed of 11 morphologically closely related species. Several viral and bacterial pathogens, and particularly Coxiella-like endosymbiont organisms have been described coexisting with ticks of this group. Since it last report in 1983, the presence of O. capensis s. l. in Brazil has remained undocumented. By a morphological analysis of larvae and a molecular characterization of ticks and Coxiella genes we describe for the first time O. capensis sensu stricto in Brazil from specimens collected on Queimada Grande Island, in São Paulo state.

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Aleurocanthus spiniferus (Hemiptera: Aleyrodidae) in Some European Countries: Diffusion, Hosts, Molecular Characterization, and Natural Enemies

Insects ◽

10.3390/insects11010042 ◽

2020 ◽

Vol 11 (1) ◽

pp. 42 ◽

Cited By ~ 2

Author(s):

Francesco Nugnes ◽

Stefania Laudonia ◽

Giovanni Jesu ◽

Maurice Gerardus Maria Jansen ◽

Umberto Bernardo ◽

...

Keyword(s):

Molecular Characterization ◽

Natural Enemies ◽

Host Plants ◽

Biocontrol Agent ◽

First Record ◽

New Host ◽

Ladybird Beetle ◽

Albanian Population ◽

First Time

After the first record in 2008 in Southeast Italy, the alien invasive and quarantine pest Aleurocanthus spiniferus (orange spiny whitefly—OSW) has gradually spread throughout Europe, infesting several new host plants in addition to the known hosts. Molecular characterization of some Italian populations and a newly found Albanian population highlighted two different haplotypes invading Europe, belonging to one of the haplogroups previously recorded in China. A predator was recorded for the first time in several fields in Italy in association with OSW and other whitefly species. It was successively identified through a morpho-molecular characterization as a Nearctic member of the tribe Serangiini, the ladybird beetle, Delphastus catalinae. This predator represents a promising biocontrol agent to manage A. spiniferus outbreaks in Italy and other invaded countries.

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Molecular and phylogenetic analyses of the liver amphistome Explanatum explanatum (Creplin, 1847) Fukui, 1929 in ruminants from Bangladesh and Nepal based on nuclear ribosomal ITS2 and mitochondrial nad1 sequences

Journal of Helminthology ◽

10.1017/s0022149x16000420 ◽

2016 ◽

Vol 91 (4) ◽

pp. 497-503 ◽

Cited By ~ 4

Author(s):

U.K. Mohanta ◽

H.B. Rana ◽

B. Devkota ◽

T. Itagaki

Keyword(s):

Molecular Characterization ◽

Phylogenetic Analyses ◽

Economic Loss ◽

Fixation Index ◽

Its2 Region ◽

Group I ◽

Severe Liver Damage ◽

Second Internal Transcribed Spacer ◽

First Time

AbstractExplanatum explanatum flukes, liver amphistomes of ruminants, cause significant economic loss in the livestock industry by inducing severe liver damage. A total of 66 flukes from 26 buffaloes and 7 cattle in four different geographic areas of Bangladesh and 20 flukes from 10 buffaloes in the Chitwan district of Nepal were subjected for analysis. The sequences (442 bp) of the second internal transcribed spacer (ITS2) of ribosomal DNA and the variable fragments (657 bp) of mitochondrial nicotinamide dehydrogenase subunit 1 (nad1) of E. explanatum flukes from Bangladesh and Nepal were analysed. The aim of this study was molecular characterization of the flukes and to elucidate their origin and biogeography. In the ITS2 region, two genotypes were detected among the flukes from Bangladesh, while flukes from Nepal were of only one genotype. Phylogenetic analyses inferred from the nad1 gene revealed that at least four divergent populations (groups I–IV) are distributed in Bangladesh, whereas two divergent populations were found to be distributed in Nepal. Fst values (pairwise fixation index) suggest that Bangladeshi and Nepalese populations of group I to IV are significantly different from each other; but within groups III and IV, the populations from Bangladesh and Nepal were genetically close. This divergence in the nad1 gene indicates that each lineage of E. explanatum from diverse geography was co-adapted during the multiple domestication events of ruminants. This study, for the first time, provides molecular characterization of E. explanatum in Bangladesh and Nepal, and may provide useful information for elucidating its origin and dispersal route in Asia.

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Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops

Agronomía Colombiana ◽

10.15446/agron.colomb.v38n2.82975 ◽

2020 ◽

Vol 38 (2) ◽

pp. 218-225

Author(s):

Diana Marcela Rivera-Toro ◽

Juan Carlos Vaca-Vaca ◽

Karina López-López

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Molecular Characterization ◽

Aphis Gossypii ◽

Chili Pepper ◽

Specific Primers ◽

Capsicum Spp ◽

Valle Del Cauca ◽

First Time

The chili pepper (Capsicum spp. L.) is a vegetable of economic importance that has been affected worldwide by the cucumber mosaic virus (CMV), a pathogen that causes a devastating disease in this crop. The aim of this research was the detection and characterization of CMV in chili pepper crops in Valle del Cauca, Colombia. Leaves of three chili pepper varieties (tabasco, cayenne and habanero) with viral symptoms were collected in four municipalities of Valle del Cauca. Total RNA was purified and a fragment of capsid protein (CP) from CMV was amplified by RT‑PCR. Then, it was sequenced and bioinformatically analyzed, and from these sequences, specific primers were designed. From 71 chili pepper samples collected in Palmira, Yumbo, Vijes and Yotoco, 37 were positive for CMV (52.1%). The CMV chili pepper sequence analysis showed that they had their highest identity (98.5%) with a CMV isolated from bananas in Ecuador. Specific primers designed for CMV chili pepper showed greater sensitivity for detecting this virus (64.7% vs. 52.1%). The CMV chili pepper CP analysis indicated that it could be transmitted by the species Aphis gossypii. This r the first time, the molecular characterization of CMV in three chili pepper varieties.

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First Report on the Detection and Molecular Characterization of Bovine Herpesvirus 1 from a Clinical case of Infectious Bovine Rhinotracheitis in Pakistan

Pakistan Veterinary Journal ◽

10.29261/pakvetj/2020.084 ◽

2021 ◽

Vol 41 (01) ◽

pp. 160-162

Author(s):

Aayesha Riaz

Keyword(s):

Molecular Characterization ◽

Nested Pcr ◽

Bovine Herpesvirus ◽

Nasal Discharge ◽

Infectious Bovine Rhinotracheitis ◽

Bovine Herpesvirus 1 ◽

Nasal Swabs ◽

Herpesvirus 1 ◽

First Time

Bovine herpesvirus 1 (BHV-1) is a noteworthy reason for many Cattle/Buffalo diseases. Infectious bovine rhinotracheitis (IBR) is one of the diseases which are caused by the BHV-1. In the present study a cow which was suspected of IBR was examined. The animal was suffering from fever and respiratory distress along with rhinitis (red nose), in appetence, and dyspnea. The nasal mucosa and muzzle were distinctly inflamed, with nasal discharge. DNA from blood samples and nasal swabs were subjected to nested PCR using glycoprotein B gene (gB) Primers. The samples were found positive for BHV-1 gB gene. Sequencing and phylogenetic analysis revealed close similarities with other BHV-1 gB gene sequences. The accession numbers assigned to this pioneer sequences in GenBank are MT449510 (BHV-1-Pak 1) and MT449511 (BHV-1-Pak 2). In this study, we reported for the first time the detection of DNA of BHV-1 through nested PCR assay and molecular characterization of BHV-1 gB gene in Pakistan. This study will be useful in further diagnoses of BHV-1 in Pakistan and in development of BHV-1 vaccine to reduce economical losses due to IBR

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Medulloblastoma

10.1093/med/9780190696696.003.0006 ◽

2018 ◽

Author(s):

Robert North ◽

Ganesh Rao

Keyword(s):

Diagnostic Criteria ◽

Molecular Characterization ◽

Survival Rates ◽

Clinical Behavior ◽

Pathological Classification ◽

Clinical Scenarios ◽

Tumor Types ◽

Classification And Treatment ◽

Brain Malignancy

Medulloblastoma is the most common brain malignancy in children. This chapter details the current diagnostic criteria, pathological classification, and treatment paradigms. Recent molecular characterization of medulloblastoma has revealed significant variations in clinical behavior of different tumor types. While the treatments for medulloblastoma are generally quite successful, with 5 year survival rates approaching 80%, the responsiveness of each subtype to treatment varies. The authors cover common clinical scenarios along with management pearls and key references.

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