Effect of supplemental lactic acid bacteria on growth performance, glutathione turnover and aflatoxin B1 removal in lambs
This study investigated the effect of lactic acid bacteria (LAB) on growth performance, glutathione turnover, aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) residue and AFB<sub>1</sub>-DNA adduct in growing lambs. Diets were a 2 × 2 factorial design, including AFB<sub>1</sub> at 0 or 100 µg/kg and LAB at 0 or 3 × 109 cfu/kg. Results showed that AFB<sub>1</sub> decreased (P < 0.05) feed intake (FI), body weight gain (BWG), and activities of glutathione (GSH), glutathione s-transferases (GSTs) and glutathione reductase (GR) in liver and duodenal mucosa, whereas these parameters were increased (P < 0.05) by supplemental LAB. AFB<sub>1</sub> increased and LAB decreased the feed conversion ratio (P < 0.05). Interactions (P < 0.05) were found on BWG, liver GSTs and mucosal GSH. LAB decreased (P ≤ 0.001) AFB<sub>1</sub> residue in liver, kidney, plasma and faeces and AFB<sub>1</sub>-DNA adduct in kidney, plasma and faeces. It can be concluded that LAB can partially protect against AFB<sub>1 </sub>toxicity by facilitating glutathione turnover and reducing AFB<sub>1</sub> toxicity in lambs.