scholarly journals Effects of ozonation on water quality and pikeperch (Sander lucioperca) performance in a recirculating aquaculture system

2021 ◽  
Vol 66 (No. 9) ◽  
pp. 381-390
Author(s):  
Jitka Kolářová ◽  
Jiří Křišťan ◽  
Oleksandr Malinovskyi ◽  
Josef Velíšek ◽  
Alžběta Stará ◽  
...  
Keyword(s):  
Water Quality ◽  
Oxygen Demand ◽  
Thiobarbituric Acid ◽  
Treatment Method ◽  
Ozone Treatment ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Sander Lucioperca ◽  
Aquaculture System ◽  
Recirculation Aquaculture System

The aim of this study was to examine the effects of ozonation on the water quality, and growth, blood biochemistry, antioxidant capacity and survival of pikeperch (Sander lucioperca) reared in a recirculation aquaculture system for 30 weeks. A group without ozone treatment was used as a control. The ozone application led to a significant reduction of the water chemical oxygen demand, biological oxygen demand and unsuspended solids concentration. The results revealed that an ozone treatment as a water treatment method has a positive influence on the intensive culture of pikeperch ensuring a higher survival rate (77%) compared to the non-treated control group (67.2%). Moreover, the ozonation prevented fin damage to a large extent and reduced the prevalence of an Ichthyophthirius multifiliis infection. Furthermore, the ozone application led to a reduction in the thiobarbituric acid reactive substance level and enhanced the superoxide dismutase activity in the fish gills. However, the effect of ozonation was null on the plasma biochemical parameters. Overall, these findings suggest that an ozone treatment, using adequate technological equipment to destroy the residual ozone, improves the water quality and protects pikeperch against any possible infection and fin damage in a recirculation aquaculture system.

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome

2011 ◽  
Vol 14 (3) ◽  
pp. 443-448 ◽  
Author(s):  
N. Kurhalyuk ◽  
H. Tkachenko ◽  
K. Pałczyńska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Tbars Level ◽  
Reactive Substance ◽  
Brown Trout Salmo Trutta

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome In the present work we evaluated the effect of ulcerative dermal necrosis (UDN) syndrome on resistance of erythrocytes to haemolytic agents and lipid peroxidation level in the blood from brown trout (Salmo trutta m. trutta L.). Results showed that lipid peroxidation increased in erythrocytes, as evidenced by high thiobarbituric acid reactive substance (TBARS) levels. Compared to control group, the resistance of erythrocytes to haemolytic agents was significantly lower in UDN-positive fish. Besides, UDN increased the percent of hemolysated erythrocytes subjected to the hydrochloric acid, urea and hydrogen peroxide. Results showed that UDN led to an oxidative stress in erythrocytes able to induce enhanced lipid peroxidation level, as suggested by TBARS level and decrease of erythrocytes resistance to haemolytic agents.

scholarly journals Evaluation of theIn VitroandIn VivoAntioxidant Potentials ofSudarshanaPowder

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Weerakoon Achchige Selvi Saroja Weerakoon ◽  
Pathirage Kamal Perera ◽  
Dulani Gunasekera ◽  
Thusharie Sugandhika Suresh
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Thiobarbituric Acid ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Trolox Equivalent ◽  
Antioxidant Effects

Sudarshanapowder (SP) is one of the most effective Ayurveda powder preparations for paediatric febrile conditions. The objective of the present study was to evaluate thein vitroandin vivoantioxidant potentials of SP. Thein vitroantioxidant effects were evaluated using ABTS radical cation decolourization assay where the TROLOX equivalent antioxidant capacity (TEAC) was determined. Thein vivoantioxidant activity of SP was determined in Wistar rats using the Lipid Peroxidation (LPO) assay in serum. Thein vitroassay was referred to as the TROLOX equivalent antioxidant capacity (TEAC) assay. For thein vivoassay, animals were dosed for 21 consecutive days and blood was drawn to evaluate the MDA level. Thein vitroantioxidant activity of 0.5 μg of SP was equivalent to 14.45 μg of standard TROLOX. The percentage inhibition against the radical formation was50.93±0.53%. The SP showed a statistically significant (p<0.01) decrease in the serum level of thiobarbituric acid-reactive substance in the test rats when compared with the control group. These findings suggest that the SP possesses potent antioxidant activity which may be responsible for some of its reported bioactivities.

Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys

2016 ◽  
Vol 57 (1-2) ◽  
pp. 10-21 ◽  
Author(s):  
Julia Kalenski ◽  
Elina Mancina ◽  
Pascal Paschenda ◽  
Christian Beckers ◽  
Christian Bleilevens ◽  
...  
Keyword(s):  
Fractional Excretion ◽  
Thiobarbituric Acid ◽  
Warm Ischemia ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Machine Perfusion ◽  
Acid Base Balance ◽  
Fractional Excretion Of Sodium ◽  
Urine Production ◽  
Systemic Oxygen

Background/Aim: The global shortage of donor organs for transplantation has necessitated the expansion of the organ pool through increased use of organs from less ideal donors. Venous systemic oxygen persufflation (VSOP) and oxygenated machine perfusion (OMP) have previously demonstrated beneficial results compared to cold storage (CS) in the preservation of warm-ischemia-damaged kidney grafts. The aim of this study was to compare the efficacy of VSOP and OMP for the preservation of warm-ischemia-damaged porcine kidneys using the recently introduced Ecosol preservation solution compared to CS using Ecosol or histidine-tryptophan-ketoglutarate solution (HTK). Materials and Methods: Kidneys from German Landrace pigs (n = 5/group) were retrieved and washed out with either Ecosol or HTK after 45 min of clamping of the renal pedicle. As controls, kidneys without warm ischemia, cold stored for 24 h in HTK, were employed. Following 24 h of preservation by VSOP, OMP, CS-Ecosol, or CS-HTK, renal function and damage were assessed during 1 h using the isolated perfused porcine kidney model. Results: During reperfusion, urine production was significantly higher in the VSOP and OMP groups than in the CS-HTK group; however, only VSOP could demonstrate lower urine protein concentrations and fractional excretion of sodium, which did not differ from the non-warm-ischemia-damaged control group. VSOP, CS-Ecosol, and controls showed better maintenance of the acid-base balance than CS-HTK. Reduced lipid peroxidation, as reflected in postreperfusion tissue thiobarbituric acid-reactive substance levels, was observed in the VSOP group compared to the OMP group, and the VSOP and CS-Ecosol groups had concentrations similar to the controls. The ratio of reduced to oxidized glutathione was higher in the VSOP, OMP, and CS-Ecosol groups than in the CS-HTK group and controls, with a higher ratio in the VSOP than in the OMP group. Conclusion: VSOP was associated with mitigation of oxidative stress in comparison to OMP and CS. Preservation of warm-ischemia-damaged porcine kidneys by VSOP was improved compared to OMP and CS, and was comparable to preservation of non-warm-ischemia-damaged cold-stored kidneys.

Nephrotoxicity in rats induced by chlorpryfos-ethyl and ameliorating effects of antioxidantsy

2002 ◽  
Vol 21 (4) ◽  
pp. 223-230 ◽  
Author(s):  
M Oncu ◽  
F Gultekin ◽  
E Karaöz ◽  
I Altuntas ◽  
N Delibas
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Mononuclear Cells ◽  
Kidney Tissue ◽  
Thiobarbituric Acid ◽  
Glomerular Sclerosis ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
High Doses ◽  
Chlorpyrifos Ethyl

Nephrotoxicity induced by chlorpyrifos-ethyl (CE) and ameliorating effects of melatonin and vitamin E plus vitamin C were evaluated in rats exposed to CE. Experimental groups were as follows: control (C), CE treated (CE), vitamin E plus vitamin C treated (Vit), melatonin treated (Mel), vitamin E plus vitamin C plus CE treated (Vit+CE), and melatonin plus CE treated (Mel+CE). The rats in the CE, Vit+CE and Mel+CE groups were administered orally with CE in two equal doses of 41 mg/kg body weight (0.25 LD50). Melatonin and vitamins E and C were administrated intramuscularly at the doses of 10, 150 and 200 mg/kg, respectively. The levels of thiobarbituric acid reactive substance (TBARS) and antioxidant potential (AOP), and the activities of glutathione peroxidase (GSHPx), catalase (CAT) and superoxide dismutase (SOD) were studied in the homogenates of kidney tissue. There were no significant differences in the activities of SOD and CAT between the experimental groups. The level of TBARS increased significantly (P<0.05) while AOP decreased significantly (P<0.05) in the CE group compared with the C group. GSH-Px activity was significantly (P<0.05) lower in the CE group and higher in the melatonin group than the control group. Histopathological changes were found in the kidney tissue of rats treated with CE. These were infiltration in mononuclear cells at perivascular and peritubular areas, hydropic degenerations in tubule epithelium and glomerular sclerosis. The severity of the lesions was reduced by administration of vitamins and melatonin. These results suggest that CE increases lipid peroxidation and decreases AOP by increasing oxidative stress, and that high doses of melatonin and a combination of vitamin E plus vitamin C considerably reduce the toxic effect of CE on kidney tissue of rats.

scholarly journals THE ANTI-OXIDANT ACTIVITIES OF ETHANOL EXTRACT OF MAHOGANY (Sweitenia mahagoni Jacq.) SEEDS IN THE LIVER TISSUES OF DIABETIC EXPERIMENTAL RATS

2017 ◽  
Vol 11 (2) ◽  
Author(s):  
Eka Prasetiawan ◽  
I Ketut Mudite Adnyane ◽  
Tutik Wresdiyati
Keyword(s):  
Ethanol Extract ◽  
Thiobarbituric Acid ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Sprague Dawley ◽  
Anti Oxidant ◽  
Oxidant Content ◽  
Liver Tissues

The aim of this research was to analyze the anti-oxidant activities of ethanol extract of mahogany seeds on the liver tissues of diabetic experimental rats to overcome oxidative stress condition. This study used male Rattus norvegicus strain Sprague Dawley which were divided into 5 groups: (i) negative control group (K-), (ii) positive control group/diabetes mellitus (DM) (K+), (iii) DM group treated with 500 mg/kg BW ethanol extract of mahogany seeds (EM), (iv) DM group treated with acarbose (KO), and (v) non-DM group treated with 500 mg/kg BW ethanol extract of mahogany seed (KE). Alloxan with dose of 110 mg/kg BW was used to induce diabetes in rats. The treatments were conducted for 28 days. At the end of treatment, the liver tissues were collected and analyzed for malondialdehyde (MDA) content using thiobarbituric acid reactive substance (TBARS) method and anti-oxidant cooper zinc superoxide dismutase (Cu,Zn-SOD) content using immunohistochemical technique. The result showed that level of MDA were significantly different (P<0.05) in K-; K+; EM; KO; and KE with values were 0.81±0.17; 1.19±0.26; 1.10±0.04; 0.95±0.13; and 0.92±0.0 µmol/gram, respectively. The anti-oxidant content (Cu, Zn-SOD) of rat liver tissue in K+ lower than in K-, while anti-oxidant content in EM and KE were higher compared to K+. The study concluded that ethanol mahogany seeds extract decreased blood glucose levels and increased the anti-oxidant status in the liver tissues of diabetic rats.

Quality and stability of meat obtained from broiler chickens fed diets containing tonic root (Mondia whitei) as supplement

2020 ◽  
Vol 47 (3) ◽  
pp. 99-109
Author(s):  
K. A. Sanwo ◽  
A. V. Adegoke ◽  
O. Y. Ayo – Ajasa ◽  
A. O. Fagbohun ◽  
T. M. Adeyanju
Keyword(s):  
Broiler Chickens ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Thiobarbituric Acid ◽  
Quality Parameters ◽  
Control Group ◽  
Broiler Chicks ◽  
Thiobarbituric Acid Reactive Substance ◽  
Treatment Groups ◽  
Inclusion Level

Tonic root has been speculated to improve Libido and this could have effect on performance and carcass traits of the animal. Afifty-six days experiment was conducted to determine meat quality of Abor acre Strain of broiler chickens fed graded dietary inclusion of supplemental white ginger (Mondia whitei). One hundred and eighty, one day-old broiler chicks were assigned to five treatment groups with three replicates per group. Birds on treatment one were fed concentrate with no supplements, those on treatment two had concentrate + 0.5g Mondia whitei per kg feed, treatment three had concentrate + 1g Mondia whitei per kg feed; Treatment 4 had concentrate with 1.5g Mondia whitei per kg feed and treatment five had concentrate + 2g Mondia whitei per kg feed. At the end of the eight weeks feeding trial, three birds each from each replicate of five treatment groups were randomly selected, deprived of food for 12 hours prior to slaughtering. After the traditional slaughtering method, feather plucking and evisceration were done and breast muscles were excised from the carcasses for quality parameters evaluation. The results showed significance (P< 0.05) in some measured parameters such as colour, yellowness (b*) and Lightness (L*). Highest (P<0.05) triglyceride content of meat (145.33 mg/dL) was recorded in the meat of birds fed inclusion level at 0.5g/kg and the lowest value (91.67mg/dL) in the meat of birds fed inclusion level of 1g/kg. Breast muscles had the highest (P<0.05) pH score 6.72 at 1g/kg inclusion level and least value of 6.51 was in the control group. Dietary inclusion of Mondia whitei showed no significant (P>0.05) effect on cholesterol, high density lipoprotein (HDL) and low-density lipoprotein (LDL). Although superoxide dismutase (SOD) and water absorptive power were not significantly (P>0.05) influenced, cooking and refrigeration loss as well as thiobarbituric acid reactive substance (TBARS) in meat were significantly (P<0.05) lowest in birds fed 1g/kg dietary inclusion level of Mondia whitei. Some phytochemicals and overall sensory acceptability were statistically influenced by the dietary treatments.

scholarly journals Water quality assessment of the Sinos River – RS, Brazil

2015 ◽  
Vol 75 (4 suppl 2) ◽  
pp. 62-67 ◽  
Author(s):  
C. Steffens ◽  
C. R. Klauck ◽  
T. Benvenuti ◽  
L. B. Silva ◽  
M. A. S. Rodrigues
Keyword(s):  
Water Quality ◽  
Water Samples ◽  
Oxygen Demand ◽  
Industrial Effluents ◽  
Water Quality Assessment ◽  
Fecal Coliforms ◽  
Control Group ◽  
The Public ◽  
Social And Economic Development ◽  
Physical And Chemical

Worldwide environmental pollution is increasing at the same rate as social and economic development. This growth, however, is disorganized and leads to increased degradation of water resources. Water, which was once considered inexhaustible, has become the focus of environmental concerns because it is essential for life and for many production processes. This article describes monitoring of the water quality at three points along the Sinos River (RS, Brazil), one in each of the upper, middle and lower stretches. The points were sampled in 2013 and again in 2014. The water samples were analyzed to determine the following physical and chemical parameters plus genotoxicity to fish: metals (Cr, Fe, Al), chemical oxygen demand, biochemical oxygen demand, chlorides, conductivity, total suspended solids, total phosphorous, total and fecal coliforms, pH, dissolved oxygen, turbidity, total Kjeldahl nitrogen nitrate and ammoniacal nitrogen. Genotoxicity was tested by exposing individuals of the species Astyanax jacuhiensis to water samples and then comparing them with a control group exposed to water from the public water supply. The results confirmed the presence of substances with genotoxic potential at the sample points located in the middle and lower stretches of the river. The results for samples from the upper stretch, at P1, did not exhibit differences in relation to the control group. The physical and chemical analyses did not detect reductions in water quality in the lower stretch, as had been expected in view of the large volumes of domestic and industrial effluents discharged into this part of the river.

Protective effects of Gingko biloba on thioacetamide-induced fulminant hepatic failure in rats

2006 ◽  
Vol 25 (12) ◽  
pp. 705-713 ◽  
Author(s):  
M M M Harputluoglu ◽  
U Demirel ◽  
H Ciralik ◽  
I Temel ◽  
S Firat ◽  
...  
Keyword(s):  
Fulminant Hepatic Failure ◽  
Hepatic Failure ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Protective Effects ◽  
Liver Necrosis ◽  
Gingko Biloba ◽  
Liver Tbars

Gingko biloba (GB) has antioxidant and platelet-activating factor (PAF) antagonistic effects. We investigated the protective effects of GB on thioacetamide (TAA)induced fulminant hepatic failure in rats. Fulminant hepatic failure was induced in treatment groups by three intraperitoneal (ip) injections of TAA (350 mg/kg) at 24-hour intervals. Treatments with GB (100 mg/kg per day, orally) and N-acetylcysteine (20 mg/kg twice daily, sc) were initiated 48 hours prior to TAA administration. The liver was removed for histopathological examinations. Serum and liver thiobarbituric acid-reactive substance (TBARS) levels were measured for assessment of oxidative stress. Liver necrosis and inflammation scores and serum and liver TBARS levels were significantly higher in the TAA group compared to the control group (P <0.001,<0.001, 0.001,<0.001, respectively). Liver necrosis and inflammation scores and liver TBARS levels were significantly lower in the GB group compared to the TAA group (P <0.001,<0.001 and 0.01, respectively). GB ameliorated hepatic damage in TAA-induced fulminant hepatic failure. This may be due to the free radical-scavenging effects of GB.

scholarly journals Evaluation of the Effect of Ceftriaxone on Lipid Peroxidation and Antioxidant Levels in Mice

2019 ◽  
Vol 12 (1) ◽  
pp. 245-250
Author(s):  
Kunjumon Dayana ◽  
Megaravalli R. Manasa
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Antioxidant Enzymes ◽  
Free Radicals ◽  
Defense Mechanisms ◽  
Thiobarbituric Acid ◽  
Generation Cephalosporin ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Albino Mice

Lipid peroxidation generates free radicals. These free radicals are scavenged by antioxidant defense mechanisms. An imbalance between the free radicals generation and antioxidant mechanisms can result in tissue damage. Several drugs are known to induce lipid peroxidation which can be responsible for their toxic potential. Hence the current study was planned to assess the effect of ceftriaxone, a third generation cephalosporin, on lipid peroxidation and levels of antioxidants in albino mice. Ceftriaxone was injected intraperitoneally at two doses - 100 mg/kg body weight; 200 mg/kg body weight – to albino mice. TBARS (Thiobarbituric acid reactive substance) levels in plasma, erythrocytes as well as tissue and the antioxidant enzymes activities were estimated. The data from ceftriaxone groups was analyzed with control group using ANOVA and Dunnett’s test as post hoc. Ceftriaxone (100 mg/kg body weight) did not alter TBARS levels compared to control. Ceftriaxone - 200 mg/kg body weight, has significantly increased TBARS levels. The activities of antioxidant enzymes were significantly decreased by ceftriaxone at these doses. The present study demonstrates that ceftriaxone has the potential for lipid peroxidation induction and reduction in the antioxidant enzymes acitivities in albino mice.

scholarly journals The Role of Filter with Different Media Compositions on Water Quality and Survival of Pangasius (Pangasius sp.) in Recirculation Aquaculture System

Omni-Akuatika ◽  
2021 ◽  
Vol 17 (1) ◽  
pp. 8
Author(s):  
Ariska Nur Oktavia ◽  
Sri Hastuti ◽  
Dicky Harwanto
Keyword(s):  
Water Quality ◽  
Ammonia Nitrogen ◽  
Average Weight ◽  
Market Demand ◽  
Feeding Rates ◽  
Feed Conversion ◽  
Filter Media ◽  
Aquaculture System ◽  
Mechanical Filter ◽  
Recirculation Aquaculture System

The high market demand for Pangasius (Pangasius sp.) has encouraged farmers to adopt a cultivation system with high stocking densities and high feeding rates. This causes fish farming waste to increase and ammonia in the waters also increases, resulting in a decrease in water quality. One of the efforts to overcome this problem is by using Recirculation Aquaculture System (RAS). This study aims to examine the effect of filters with different media compositions in RAS of Pangasius. Test fish, average weight of 4.59 g, kept for 30 days in aquarium with a density of 1.15 g.L-1. The feed is given at satiation, twice a day. There were five treatments and four replications during study. The treatments were the difference composition of filter media, i.e. A (100% zeolite), B (25% net + 75% zeolite), C (50% net + 50% zeolite), D (75% net + 25% zeolite), and E (100% net). Dacron was used as a mechanical filter in all treatments. The results showed that the composition of different filter materials had a significant effect (P <0.05) on Survival Rate (SR), Total Amount of Feed Fed (TFF), Relative Growth Rate (RGR), and Feed Conversion Efficiency (FCE). The best performance was showed by media biofilter composition of 25% nets + 75% zeolite, with the value of VTR (Volumetric Total Ammonia Nitrogen Removal) were 55.45-66.32 g.m-3.day-1, SR was 93.75%; TFF was 124.84 g; RGR was 2.13%.day-1, and FCE was 46.87%. Various different compositions of net and zeolite as filter media on RAS were able to manage TAN below tolerance limit.Keywords: catfish, recirculation system, filter composition, dacron, net, zeolite.

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