The Lower Yom River Basin is located in the north of Thailand. This study carried out to calibrate and validate using SWAT model in terms of streamflow and sediment concentration hydrographs (Year 2000-2012) for 3 RID streamflow gauging stations (the Royal Irrigation Department). The nitrates concentrations simulate have been influenced of land use changes during last ten years. Optimal values of model parameters derived from calibration and validation processes, which showed well fitted between observed and simulated results. In the last decade, particular in Lower Yom River, the land use change gradually transformed to be more paddy field and has been increased 127.48 km2 (approx. 0.87% increase), followed by urban area, which has been increased 196.66 km2 (approx. 1.35% increase), respectively. Average monthly concentration of nitrate increased 38.28 mg/l (approx.13.40 % increase), 43.17 mg/l (approx.12.00% increase), 43.02 mg/l (approx. 8.60% increase) at station Y.6, Y.4 and Y.17, respectively. Accordingly, on the basis of the results presented in this study, land use changes can significantly affect on concentrations of nitrate.