Oral Supplementation of Bauhinia variegata affects the Behavior of Albino Mice in a Gender Specific Manner

Aim of this study was to report the biocompatibility of Holmium zirconate, zinc sulphide and holmium zirconate/zinc sulphide (HO2Zr2O7/ZnS) nanocomposite in albino mice. Holmium zirconate, zinc sulphide and holmium zirconate/zinc sulphide (Ho2Zr2O7/ZnS) nanocomposite were synthesized by, normal microemulsion, chemical co-precipitation and two step synthesis method, respectively. The synthesized materials were characterized by X-ray diffraction (XRD) for the confirmation of phase while scanning electron microscopy (SEM) was used morphological analysis. The composition and the particle size distribution were confirmed by energy dispersive spectroscopy and particle size analysis respectively. Seven week old mice were divided into two groups in a gender specific manner: control group that were intraperitoneally injected with saline solution and treated group were administered with 50 mg/ml solvent/Kg body weight of Holmium zirconate/zinc sulphide nanocomposite for 22 days. A series of neurological tests, blood cell count, selected serum parameters and biomarkers of oxidative stress were analysed in vital organs of both treatments. It was observed that nanocomposite treated female mice remained mobile (P = 0.05) for longer time while both male (P = 0.03) and female (P = 0.02) mice had more rotations than saline treated mice during open field test. Nanocomposite treated male had reduced stretch attend reflex during light dark box test. Blood and serum parameters remained unaffected (P ˃ 0.05) when compared between nanocomposite treated and untreated mice of both genders. Malondialdehyde concentration was significantly elevated (P = 0.04) in liver of male while superoxide dismutase concentrations were significantly reduced (P = 0.05) in brain of female albino mice treated with nanocomposite than their respective control groups.

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Effects of Methanolic Leaf Extracts of Daniella oliveri on Biochemical and Haematological Parameters of Albino Mice Infected with Plasmodium berghei NK 65

Journal of Advances in Microbiology ◽

10.9734/jamb/2021/v21i530348 ◽

2021 ◽

pp. 22-32

Author(s):

Muhammed Muazu ◽

Karderam Bukar Dikwa ◽

Deborah Madi Dibal ◽

Muhammed Danjuma ◽

Gideon Obaje Sunday ◽

...

Keyword(s):

Body Weight ◽

Normal Control ◽

Plasmodium Berghei ◽

Leaf Extract ◽

Hematological Parameters ◽

Negative Control ◽

Haematological Parameters ◽

Control Group ◽

Leaf Extracts ◽

Albino Mice

The rapid emergence and spread of Plasmodium falciparum resistance to Artemisinin derivatives and all the conventional antimalarial drugs necessitates the importance of ethnobotany, resulting in need to study the antiplasmodial potentials and the resultant effects of the methanolic leaf extract of Daniella oliveri (D.oliveri) on the biochemical and haematological parameters of the infected and treated albino mice. A total of 30 mice were randomized to six groups; 1 (positive control), 2 (negative control), 3 (normal control), 4, 5 and 6 (treatment groups) of five mice per group, body weights of mice were measured before and after infection and treatments, the mice were Infected intravenously with 0.2 ml of 1x107 standard inoculum of chloroquine sensitive Plasmodium berghei infected erythrocytes on the first day (day 0), treatment commence 72 hours later (day 3), continued for 5 days to terminate on day 7. On day 8, the Swiss Albino mice used for antiplasmodial activity were subjected to euthanasia under chloroform, aseptically dissected and blood was collected through cardiac puncture in lithium heparin bottle for biochemical assays and in an ethylene diamine tetra- acetic acid (EDTA) bottles for haematological assays.All mice in the treatment group showed decrease in body weight except for normal control group that showed increase in body weight. Methanolic leaf extract of D.oliveri contains some secondary metabolites that are hepato-protective in nature with no significant effects on the biochemical and hematological parameters of the malaria infected and treated albino mice.

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Oral supplementations of Bauhinia variegata’s leaf extract has anxiolytic effect and improves locomotory and exploratory behavior of female albino mice

International Journal of Phytomedicine ◽

10.5138/09750185.2073 ◽

2017 ◽

Vol 9 (2) ◽

Author(s):

Furhan Iqbal ◽

Muhammad Jahangir ◽

Asmat Ullah ◽

Muhammad Naseer Khan Khattak ◽

Shahid Iqbal

Keyword(s):

Body Weight ◽

Open Field ◽

Adult Female ◽

Exploratory Behavior ◽

Leaf Extract ◽

Elevated Plus Maze ◽

Anxiolytic Effect ◽

Elevated Plus Maze Test ◽

Plus Maze ◽

Albino Mice

Present study was designed to investigate the effects of 200 mg/ml solvent/Kg of body weight of Bauhinia variegata’s leaf extract on neuromuscular coordination, locomotory and exploratory behavior of adult female albino mice. Six week old female albino mice were used as experimental animals. Mice were either orally supplemented with 200 mg/ml/Kg of body weight Bauhinia variegata’s leaf extract or with saline solution [0.9%] for 7 days. Behavioral observations were made by applying a series of neurological tests [Rota rod, Elevated plus maze, Light and dark box, Open field and Novel object test]. Exploratory and locomotory behavior during Open Field test was improved in leaf extract treated mice as they had more means speed [P = 0.01], rotations [P = 0.001], clockwise rotations [P = 0.004] and anti-clockwise rotations [P = 0.001] than control mice. Bauhinia variegata’s leaf extract treated mice had higher transition frequency [P = 0.002] and spent more time in light compartment during light dark box test than saline treated controls. Bauhinia variegata’s leaf extract treated mice covered more distance [P = 0.033] with higher speed [P = 0.028] compared to control mice during Elevated Plus Maze test. Bauhinia variegata’s leaf extract improves the locomotory and exploratory behavior in adult female albino mice. Leaf extract of Bauhinia variegate can be further explored to be used as a therapeutic agent for the treatment of neurological ailments.

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Effect of P-alaxin on some neurobehavioural parameters in Swiss white mice

Journal of Applied and Natural Science ◽

10.31018/jans.v10i4.1947 ◽

2018 ◽

Vol 10 (4) ◽

pp. 1308-1313

Author(s):

C. O. Nku ◽

A. O. Essien ◽

A. U. Ime

Keyword(s):

Body Weight ◽

Dose Group ◽

High Dose Group ◽

Morris Water Maze Test ◽

Control Group ◽

High Dose ◽

Clinical Dose ◽

Maze Test ◽

Normal Rat ◽

Total Analysis

The effect of P-alaxin on some neurobehavioural parameters was studied in Swiss white mice. 21 mice were randomly assigned to three groups (n=7) namely; control group, clinical dose group and high dose group. Animals in control group received normal rat chow and standard feed ad libitum. Animals in the clinical dose group were given P-alaxin at a dose of 0.01mg/kg body weight while animals in the high dose group were given P-alaxin at a dose of 0.02mg/kg body weight. The results obtained showed that, in the open field maze test, the clinical dose and high dose groups showed significant increase (P<0.05) in stretch attend posture compared to control. Conversely, there was a significant decrease (P<0.05) in line crossings of high and clinical dose groups when compared to control. In the light/dark transition box test, the clinical and high dose groups showed a significant decrease (P<0.05) in the frequency of line crossings when compared to control. In the morris water maze test, the high and clinical dose groups showed a significant inncrease in frequency of swimming latency when compared to control group. From the total analysis obtained, P-alaxin decreases locomotion and exploratory activities, it impairs motivation and the tendency to investigate the environment as well as reduction in visuospartial learning and memory.

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Synthesis, Characterization and Biocompatibility of Holmium Zirconate/Zinc Sulphide Nanocomposite in Albino Mice in a Gender Specific Manner

Journal of the chemical society of pakistan ◽

10.52568/000552/jcsp/43.01.2021 ◽

2021 ◽

Vol 43 (1) ◽

pp. 46-46

Author(s):

Hafsa Noureen Hafsa Noureen ◽

Mubashra Salim Mubashra Salim ◽

Amjad Nadeem Amjad Nadeem ◽

Sana Shafiq Sana Shafiq ◽

Samia Yousuf Samia Yousuf ◽

...

Keyword(s):

Particle Size ◽

Synthesis Method ◽

Particle Size Analysis ◽

Zinc Sulphide ◽

Size Analysis ◽

Control Group ◽

Serum Parameters ◽

Specific Manner ◽

Albino Mice ◽

Gender Specific

Aim of this study was to report the biocompatibility of Holmium zirconate, zinc sulphide and holmium zirconate/zinc sulphide (HO2Zr2O7/ZnS) nanocomposite in albino mice. Holmium zirconate, zinc sulphide and holmium zirconate/zinc sulphide (Ho2Zr2O7/ZnS) nanocomposite were synthesized by, normal microemulsion, chemical co-precipitation and two step synthesis method, respectively. The synthesized materials were characterized by X-ray diffraction (XRD) for the confirmation of phase while scanning electron microscopy (SEM) was used morphological analysis. The composition and the particle size distribution were confirmed by energy dispersive spectroscopy and particle size analysis respectively. Seven week old mice were divided into two groups in a gender specific manner: control group that were intraperitoneally injected with saline solution and treated group were administered with 50 mg/ml solvent/Kg body weight of Holmium zirconate/zinc sulphide nanocomposite for 22 days. A series of neurological tests, blood cell count, selected serum parameters and biomarkers of oxidative stress were analysed in vital organs of both treatments. It was observed that nanocomposite treated female mice remained mobile (P = 0.05) for longer time while both male (P = 0.03) and female (P = 0.02) mice had more rotations than saline treated mice during open field test. Nanocomposite treated male had reduced stretch attend reflex during light dark box test. Blood and serum parameters remained unaffected (P ˃ 0.05) when compared between nanocomposite treated and untreated mice of both genders. Malondialdehyde concentration was significantly elevated (P = 0.04) in liver of male while superoxide dismutase concentrations were significantly reduced (P = 0.05) in brain of female albino mice treated with nanocomposite than their respective control groups.

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Synthesis, Characterization and Biocompatibility of Holmium Zirconate/Zinc Sulphide Nanocomposite in Albino Mice in a Gender Specific Manner

Journal of the chemical society of pakistan ◽

10.52568/000552 ◽

2021 ◽

Vol 43 (1) ◽

pp. 46-46

Author(s):

Hafsa Noureen Hafsa Noureen ◽

Mubashra Salim Mubashra Salim ◽

Amjad Nadeem Amjad Nadeem ◽

Sana Shafiq Sana Shafiq ◽

Samia Yousuf Samia Yousuf ◽

...

Keyword(s):

Particle Size ◽

Synthesis Method ◽

Particle Size Analysis ◽

Zinc Sulphide ◽

Size Analysis ◽

Control Group ◽

Serum Parameters ◽

Specific Manner ◽

Albino Mice ◽

Gender Specific

Aim of this study was to report the biocompatibility of Holmium zirconate, zinc sulphide and holmium zirconate/zinc sulphide (HO2Zr2O7/ZnS) nanocomposite in albino mice. Holmium zirconate, zinc sulphide and holmium zirconate/zinc sulphide (Ho2Zr2O7/ZnS) nanocomposite were synthesized by, normal microemulsion, chemical co-precipitation and two step synthesis method, respectively. The synthesized materials were characterized by X-ray diffraction (XRD) for the confirmation of phase while scanning electron microscopy (SEM) was used morphological analysis. The composition and the particle size distribution were confirmed by energy dispersive spectroscopy and particle size analysis respectively. Seven week old mice were divided into two groups in a gender specific manner: control group that were intraperitoneally injected with saline solution and treated group were administered with 50 mg/ml solvent/Kg body weight of Holmium zirconate/zinc sulphide nanocomposite for 22 days. A series of neurological tests, blood cell count, selected serum parameters and biomarkers of oxidative stress were analysed in vital organs of both treatments. It was observed that nanocomposite treated female mice remained mobile (P = 0.05) for longer time while both male (P = 0.03) and female (P = 0.02) mice had more rotations than saline treated mice during open field test. Nanocomposite treated male had reduced stretch attend reflex during light dark box test. Blood and serum parameters remained unaffected (P ˃ 0.05) when compared between nanocomposite treated and untreated mice of both genders. Malondialdehyde concentration was significantly elevated (P = 0.04) in liver of male while superoxide dismutase concentrations were significantly reduced (P = 0.05) in brain of female albino mice treated with nanocomposite than their respective control groups.

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Effect of Sorafenib on Sex Hormone Levels in Male Swiss Albino Mice

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00674 ◽

2021 ◽

pp. 3883-3888

Author(s):

Surekha D. Shetty ◽

Laxminarayana Bairy K. ◽

AM Prasad ◽

Satheesha Nayak B. ◽

Ashwini Aithal P.

Keyword(s):

Body Weight ◽

Semen Analysis ◽

Young Patients ◽

Differentiated Thyroid Carcinoma ◽

Positive Control ◽

Vital Role ◽

Reproductive Age ◽

Control Group ◽

Swiss Albino Mice ◽

Albino Mice

Background: Hormones play a vital role in initiating and maintenance of male reproductive or testicular function which includes the production of androgens and spermatozoa. Testosterone is essential for the initiation and maintenance of spermatogenesis. FSH is responsible for the stimulation of spermatogenesis. Semen analysis and hormone evaluation are essential parameters in the diagnosis of infertility in males. Objective: The aim of the present study is to evaluate the effect of sorafenib on FSH and intratesticular testosterone levels in male Swiss albino mice. Materials and Methods: The animals were segregated into control, positive control, and treatment groups (n=6). Treatment group received 25, 50 and 100 mg/kg body weight of sorafenib orally for seven consecutive days at intervals of 24 hours between two administrations. Positive control group received 100 mg/kg body weight of imatinib. The animals were sacrificed at the end of 1st, 2nd, 4th, 5th, 7th and 10th week after the last exposure to sorafenib. Results: The intratesticular testosterone level was significantly (P<0.05) reduced in treated groups and severe effect was observed on week 4th and 5th weeks. FSH level was increased significantly (P<0.05) in sorafenib treated groups of mice. Conclusion: The administration of sorafenib does affect testosterone and FSH level significantly, but this effect is reversible once the drug is withdrawn. This finding may help the clinicians to plan and address the fertility-related issues in young patients of reproductive age who are being treated with sorafenib for advanced renal cell carcinoma, hepatocellular carcinoma and differentiated thyroid carcinoma.

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In vivo antidiarrheal study of ethanolic extracts of Mikania cordata and Litsea monopetala leaves

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v10i3.23402 ◽

2015 ◽

Vol 10 (3) ◽

pp. 562 ◽

Cited By ~ 2

Author(s):

Fatema Nasrin ◽

Md. Lukman Hakim

Keyword(s):

Body Weight ◽

Castor Oil ◽

Leaf Extract ◽

Positive Control ◽

Control Group ◽

P Value ◽

Leaf Extracts ◽

Ethanolic Extracts ◽

Antidiarrheal Activity

In this study the antidiarrheal activity of ethanolic extracts of the leaves of Mikania cordata and Litsea monopetala was evaluated. Diarrhea was induced in mice by oral administration of castor oil (0.5 mL) 30 min after the administration of the extracts. During a 4 hour study the number of diarrheal feces and percentage inhibition of the extracts (200 and 400 mg/kg body weight) was determined. Loperamide (3 mg/kg body weight) served as standard and belonged to the positive control group. The extracts exhibited potent antidiarrheal activity as well as achieved statistically significant p value (p<0.01 and p<0.05) compared to control group. Among the extracts the highest percentage inhibition of defecation (60%) was recorded for leaf extract (400 mg/kg body weight) of L. monopetala. So, the study corroborates the significant antidiarrheal activity of M. cordata and L. monopetala leaf extracts and raises the demand of further sophisticated investigation.

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EFFECT OF ALOE BARBADENSIS MILLER WHOLE LEAF EXTRACT ON HYPERGLYCEMIA AND INSULIN RESISTANCE IN LOW DOSE STREPTOZOTOCIN INDUCED TYPE 2 DIABETIC RATS

Journal of Saidu Medical College, Swat ◽

10.52206/jsmc.2013.3.2.350-355 ◽

1969 ◽

Vol 3 (2) ◽

pp. 350-355

Author(s):

MEENA GUL ◽

MUHAMMAD MAZHAR HUSSAIN ◽

AYESHA BABER ◽

AMJAD ZAMAN ◽

MUSRAT ZAHRA

Keyword(s):

Insulin Resistance ◽

Body Weight ◽

Plasma Glucose ◽

Leaf Extract ◽

Low Dose ◽

Aloe Vera ◽

Control Group ◽

Sprague Dawley ◽

Type 2 Diabetic

BACKGROUND: Managing diabetes is difficult due to the number of side effects associated with drugsused for its treatment. There it is a need of an hour to look for indigenous plants which are safe and costeffective. Present study was planned to determine the effect of Aloe vera whole leaf extract and/orRosiglitazone on plasma glucose, insulin and insulin resistance in type 2 diabetic Sprague-Dawley rats.DESIGN: Randomized control trailPLACE AND DURATION OF STUDY: This study was conducted from April 2009 to Oct 2010 at theDepartment of Physiology Army Medical College, Rawalpindi in collaboration with National Institute ofHealth (NIH) Islamabad.MATERIAL AND METHOD: Type 2 DM was induced in 60 healthy Sprague-Dawley rats by feedinghigh fat diet for 2 weeks and injecting a low dose (35mg/kg) of streptozotocin intra peritoneally. Type 2diabetic rats were randomly divided into four groups, each group having 15 rats and were labeled as diabeticgroup, Aloe vera group, rosiglitazone group and combined group. The diabetic group was injected normalsaline, Aloe vera group was treated with Aloe vera whole leaf extract in dose of 300mg/kg body weight,rosiglitazone group was given 5mg/kg body weight of rosiglitazone I/P and combined group diabetic ratswere treated with 150mg/kg body weight of Aloevera extract and 2.5mg/kg body weight of rosiglitazone(halfof their effective dose) for 21 days.RESULTS: A significant reduction (p<0.001) in plasma glucose (73%), insulin (32%) and TG/HDL ratio(81%) was analyzed in combined groupascompared to diabetic control group. \CONCLUSION: The maximum impact in lowering plasma glucose, insulin and TG/HDL ratio wasrecorded in combined group, followed by rosiglitazone group and then Aloevera group.KEYWORDS:T2DM. Aloe vera, insulin resistance

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High-Fat Diet Enhances Working Memory in the Y-Maze Test in Male C57BL/6J Mice with Less Anxiety in the Elevated Plus Maze Test

Nutrients ◽

10.3390/nu12072036 ◽

2020 ◽

Vol 12 (7) ◽

pp. 2036 ◽

Cited By ~ 2

Author(s):

Kaichi Yoshizaki ◽

Masato Asai ◽

Taichi Hara

Keyword(s):

Working Memory ◽

Body Weight ◽

High Fat Diet ◽

Elevated Plus Maze ◽

High Fat ◽

Behavioral Traits ◽

Elevated Plus Maze Test ◽

Maze Test ◽

Y Maze ◽

Plus Maze

Obesity is characterized by massive adipose tissue accumulation and is associated with psychiatric disorders and cognitive impairment in human and animal models. However, it is unclear whether high-fat diet (HFD)-induced obesity presents a risk of psychiatric disorders and cognitive impairment. To examine this question, we conducted systematic behavioral analyses in C57BL/6J mice (male, 8-week-old) fed an HFD for 7 weeks. C57BL/6J mice fed an HFD showed significantly increased body weight, hyperlocomotion in the open-field test (OFT) and Y-maze test (YMZT), and impaired sucrose preference in the sucrose consumption test, compared to mice fed a normal diet. Neither body weight nor body weight gain was associated with any of the behavioral traits we examined. Working memory, as assessed by the YMZT, and anxiety-like behavior, as assessed by the elevated plus maze test (EPMT), were significantly correlated with mice fed an HFD, although these behavioral traits did not affect the entire group. These results suggest that HFD-induced obesity does not induce neuropsychiatric symptoms in C57BL/6J mice. Rather, HFD improved working memory in C57BL/6J mice with less anxiety, indicating that an HFD might be beneficial under limited conditions. Correlation analysis of individual traits is a useful tool to determine those conditions.

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