scholarly journals Chemical Characterization and in-vitro Antimicrobial Screening of Ethanolic Extract of Propolis Collected from Jazan, Saudi Arabia

2019 ◽  
Vol 52 (1) ◽  
Author(s):  
Mohammed Al Bratty ◽  
Hassan A. Alhazmi ◽  
Desam Nagarjuna Reddy ◽  
Abdul Jabbar Al-Rajab ◽  
Sadique A. Javed ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Antimicrobial Screening ◽  
Ethanolic Extract Of Propolis

scholarly journals The Comparative Evaluation of the Antimicrobial Effect of Propolis with Chlorhexidine against Oral Pathogens: An In Vitro Study

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
A. Eralp Akca ◽  
Gülçin Akca ◽  
Fulya Toksoy Topçu ◽  
Enis Macit ◽  
Levent Pikdöken ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Ethanolic Extract ◽  
Antimicrobial Effect ◽  
Single Species ◽  
Test Methods ◽  
Streptococcus Sobrinus ◽  
Gram Positive Bacteria ◽  
Agar Dilution ◽  
Ethanolic Extract Of Propolis

This study aimed to compare the antimicrobial effectiveness of ethanolic extract of propolis (EEP) to chlorhexidine gluconate (CHX) on planktonicStreptococcus mutans,Streptococcus sobrinus,Lactobacillus acidophilus,Lactobacillus salivariussubsp.salivarius,Aggregatibacter actinomycetemcomitans,Prevotella intermedia,Porphyromonas gingivalis,Staphylococcus aureus,Enterococcus faecalis,Actinomyces israelii,Candida albicans, and their single-species biofilms by agar dilution and broth microdilution test methods. Both agents inhibited the growth of all planktonic species. On the other hand, CHX exhibited lower minimum bactericidal concentrations than EEP against biofilms ofA. actinomycetemcomitans,S. aureus, andE. faecaliswhereas EEP yielded a better result against Lactobacilli andP. intermedia. The bactericidal and fungicidal concentrations of both agents were found to be equal against biofilms of Streptecocci,P. gingivalis,A. israelii, andC. albicans. The results of this study revealed that propolis was more effective in inhibiting Gram-positive bacteria than the Gram-negative bacteria in their planktonic state and it was suggested that EEP could be as effective as CHX on oral microorganisms in their biofilm state.

scholarly journals In Vitro Antifungal and Antivirulence Activities of Biologically Synthesized Ethanolic Extract of Propolis-Loaded PLGA Nanoparticles against Candida albicans

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Anupon Iadnut ◽  
Ketsaya Mamoon ◽  
Patcharin Thammasit ◽  
Sudjai Pawichai ◽  
Singkome Tima ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Virulence Factors ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Vero Cells ◽  
Plga Nanoparticles ◽  
Water Soluble ◽  
Free Form ◽  
Ethanolic Extract Of Propolis

Propolis is a natural substance and consists of bioactive compounds, which gives it antioxidant and antimicrobial properties. However, the use of propolis is limited by the low solubility in aqueous solutions. Thus, nanoparticles may be likely to accomplish enhanced delivery of poorly water-soluble phytomedicine. The aim of the present study was to fabricate and evaluate the biological activity of ethanolic extract of propolis-loaded poly(lactic-co-glycolic acid) nanoparticles (EEP-NPs). The EEP-NPs were prepared using the oil-in-water (o/w) single-emulsion solvent evaporation technique. The physicochemical properties of EEP-NPs were characterized and tested on their cytotoxicity, antifungal activity, and impact on key virulence factors that contribute to pathogenesis of C. albicans. EEP-NPs were successfully synthesized and demonstrated higher antifungal activity than EEP in free form. Moreover, EEP-NPs exhibited less cytotoxicity on Vero cells and suppressed the virulence factors of C. albicans, including adhesion, hyphal germination, biofilm formation, and invasion. Importantly, EEP-NPs exhibited a statistical decrease in the expression of hyphal adhesion-related genes, ALS3 and HWP1, of C. albicans. The results of this study revealed that EEP-NPs mediates a potent anticandidal activity and key virulence factors by reducing the gene-encoding virulence-associated hyphal- adhesion proteins of C. albicans and, thereby, disrupting the morphologic presence and attenuating their virulence.

scholarly journals Polyphenols from Chilean Propolis and Pinocembrin Reduce MMP-9 Gene Expression and Activity in Activated Macrophages

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Nicolás Saavedra ◽  
Alejandro Cuevas ◽  
Marcela F. Cavalcante ◽  
Felipe A. Dörr ◽  
Kathleen Saavedra ◽  
...  
Keyword(s):  
Gene Expression ◽  
Proteolytic Activity ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Ethanol Solution ◽  
Dependent Manner ◽  
Propolis Extract ◽  
Joint Contribution ◽  
Ethanolic Extract Of Propolis

Polyphenols from diverse sources have shown anti-inflammatory activity. In the context of atherosclerosis, macrophages play important roles including matrix metalloproteinases synthesis involved in degradation of matrix extracellular components affecting the atherosclerotic plaque stability. We prepared a propolis extract and pinocembrin in ethanol solution. Propolis extract was chemically characterized using LC-MS. The effect of treatments on gene expression and proteolytic activity was measuredin vitrousing murine macrophages activated with LPS. Cellular toxicity associated with both treatments and the vehicle was determined using MTT and apoptosis/necrosis detection assays. MMP-9 gene expression and proteolytic activity were measured using qPCR and zymography, respectively. Thirty-two compounds were identified in the propolis extract, including pinocembrin among its major components. Treatment with either ethanolic extract of propolis or pinocembrin inhibits MMP-9 gene expression in a dose-dependent manner. Similarly, an inhibitory effect was observed in proteolytic activity. However, the effect showed by ethanolic extract of propolis was higher than the effect of pinocembrin, suggesting that MMP-9 inhibition results from a joint contribution between the components of the extract. These data suggest a potential role of polyphenols from Chilean propolis in the control of extracellular matrix degradation in atherosclerotic plaques.

Mechanisms involved in the relaxant action of the ethanolic extract of propolis in the guinea-pig trachea in-vitro

2002 ◽  
Vol 54 (6) ◽  
pp. 845-852 ◽  
Author(s):  
Niraldo Paulino ◽  
Fernando M. Scremin ◽  
Lisiane B. Raichaski ◽  
Maria Cristina Marcucci ◽  
Amarilis Scremin ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Ethanolic Extract ◽  
Ethanolic Extract Of Propolis

scholarly journals Antimicrobial screening and hemolytic activity of products obtained from Piper montealegreanum Yuncker and effect in vitro on growth of Staphylococcus aureus

2020 ◽  
Vol 9 (9) ◽  
pp. e346997410
Author(s):  
Wilma Raianny Vieira da Rocha ◽  
Luanne Eugenia Nunes ◽  
Anna Flávia Costa Fernandes ◽  
Raïssa Mayer Ramalho Catão ◽  
Harley da Silva Alves
Keyword(s):  
Staphylococcus Aureus ◽  
Hemolytic Activity ◽  
Membrane Damage ◽  
Clinical Importance ◽  
Ethanolic Extract ◽  
Antimicrobial Screening ◽  
Staphyloccocus Aureus ◽  
Time Kill ◽  
Chcl3 Fraction

This study aimed to determine spectrum of antimicrobial activity of crude ethanolic extract (CEE), chloroform (CHCl3) and ethyl acetate (EtOAc) fractions, to determine influence of CHCl3 fraction on Staphylococcus aureus growth and hemolytic activity. Antimicrobial screening of CEE, CHCl3 and EtOAc fractions was carried out using broth microdilution technique against standard strains: Staphyloccocus aureus ATCC25923, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853 and Candida albicans ATCC76645 to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Time kill curves were performed to determine influence of CHCl3 fraction on MIC and ½ MIC concentrations on S. aureus growth. Hemolytic activity of CHCl3 was performed in human erythrocytes to report the cytotoxic effect. CHCl3 fraction showed activity against S. aureus with MIC=1024 µg mL-1 and MBC=2048 µg mL-1. This fraction did not show activity against other tested strains, as well as the CEE and EtOAc fraction. Time kill curve of S. aureus treated with CHCl3 fraction showed that at MIC this fraction has bacteriostatic activity, which was not observed with the ½ MIC. Hemolytic activity demonstrated that when CHCl3 fraction in MIC does not present membrane damage of erythrocytes, since it did not cause hemolysis. Studies should be conducted to evaluate the activity of CHCl3 fraction against S. aureus with different antimicrobial resistance profiles, in addition to evaluating the potential for resistance reversion by in vitro combination studies. The study demonstrated that the species P. montealegreanum can be an important source of compounds of importance for combating microorganisms of clinical importance.

scholarly journals In Vitro Anti Acne Activity of Ethanolic Extract of Stem of Berberis aristata

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Shyam Baboo Prasad ◽  
Darshpreet Kaur
Keyword(s):  
Research Work ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Common Disease ◽  
Inflammatory Disorder ◽  
Antimicrobial Screening ◽  
The Impact ◽  
Mic Value

Current research work is intended to study the impact of herbal approach to treat acne, an extremely common cutaneous inflammatory disorder of multifactorial origin with prevalence in adolescents. Acne is common disease of skin and is usually treatable. An attempt had been taken to investigate the in vitro antiacne activity of ethanolic extract of stem of Berberis aristata. The MIC value of the B. aristata extract against S. epidermidis, P. acnes and M. furfur were found to be 600 μg/ml, 200 μg/ml and 100 μg/ml respectively. In vitro antimicrobial screening using erythromycin as a positive control clearly indicated that ethanolic extract of B. aristata is promising antimicrobial against the test microorganisms.

scholarly journals Antimicrobial activity of a novel Spanish propolis against planktonic and sessile oral Streptococcus spp

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Luisa Navarro-Pérez ◽  
Virginia Vadillo-Rodríguez ◽  
Irene Fernández-Babiano ◽  
Ciro Pérez-Giraldo ◽  
M. Coronada Fernández-Calderón
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Oral Streptococcus ◽  
Ancient Times ◽  
Ethanolic Extract Of Propolis

AbstractIncreased bacterial resistance to traditional antimicrobial agents has prompted the use of natural products with antimicrobial properties such as propolis, extensively employed since ancient times. However, the chemical composition of propolis extracts is extremely complex and has been shown to vary depending on the region and season of collection, due to variations in the flora from which the pharmacological substances are obtained, being therefore essential for their antimicrobial activity to be checked before use. For this purpose, we evaluate the in vitro antimicrobial and anti-biofilm activity of a new and promising Spanish ethanolic extract of propolis (SEEP) on Streptococcus mutans and Streptococcus sanguinis, responsible, as dominant ‘pioneer’ species, for dental plaque. Results reveal that S. sanguinis is more sensitive to SEEP, slowing and retarding its growth considerably with lower concentrations than those needed to produce the same effect in S. mutans. SEEP presents concentration- and time-dependent killing activity and, furthermore, some of the subinhibitory concentrations employed increased biofilm formation even when bacterial growth decreased. Mono and dual-species biofilms were also inhibited by SEEP. Findings obtained clearly show the relevance of using biofilm and subinhibitory concentration models to determine optimal treatment concentrations.

scholarly journals Bioactive compounds from Syzygium malaccense leaves: optimization of the extraction process, biological and chemical characterization

2020 ◽  
Vol 42 ◽  
pp. e46773 ◽  
Author(s):  
Aline Savi ◽  
Matheus Augusto Calegari ◽  
Gabrielle Cristina Calegari ◽  
Vidiany Aparecida Queiroz Santos ◽  
Denise Wermuth ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Radical Scavenging Ability

Syzygium malaccense belongs to the family Myrtaceae and it is popularly recognised as ‘jambo’ in Brazil. It is poorly studied regarding the bioactive compounds of fruits and leaves. This study aimed at optimizing the extraction and characterization of bioactives compounds from S. malaccense leaves. Extraction was optimized using a 23 full factorial design to evaluate the effect of ethanol concentration, time and temperature on polyphenols and antioxidants contents. Analyses of phenolic compounds content (Folin-Ciocalteau and HPLC-DAD), in vitro antioxidant capacities (DPPH, ABTS and FRAP), antimicrobial activity against bacteria and yeasts of ethanolic extract was carried out in optimal conditions of extraction. The optimized extraction condition was 45 min., 80°C and 40:60 (v v-1) ethanol:water. The extract showed high total phenolic content (0.074 mg GAE kg-1), DPPH (0.666 μmol TE kg-1) and ABTS (0.853 μmol TE kg-1) free radical scavenging ability and appreciable ferric reducing power (1.267 μmol Fe2+ kg-1). HPLC methodology allowed the identification of four bioactive compounds: gallic acid (0.00036 mg kg-1), catechin (0.00021 mg kg-1), rutin (0.00027 mg kg-1) and quercetin (0.00003 mg kg-1). The extract showed inhibitory activity against S. aureus (1 µg L-1); S. bongori (8 µg L-1), C. albicans and C. tropicalis (2 µg L-1). It also showed microbicidal potential against S. aureus; C. albicans and C. tropicalis.

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