scholarly journals Morphometry, viability and germination of seeds of Benincasa hispida [(Thunb.) Cogn.]

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Miguel Esptia-Camacho ◽  
Alisson Villalba-Soto ◽  
Hermes Aramédiz-Tatis ◽  
Carlos Cardona-Ayala
Keyword(s):  
Seed Viability ◽  
Morphological Description ◽  
Germination Test ◽  
Weight Density ◽  
Benincasa Hispida ◽  
Germination Speed ◽  
Germination Value ◽  
Protective Membrane ◽  
Tetrazolium Test ◽  
Peak Value

The objective of the study was to evaluate the morphometry, viability and germination of B. hispida seeds, at the Universidad de Córdoba, Colombia. Eight biometric characteristics were measured in a sample of seeds taken at random from a balanced compound originating from 50 fruits. The external and internal morphological description was made on a sample of 10 seeds. Seed viability was estimated by the tetrazolium test, using the combination of two staining times (2 and 3 hours) and three concentrations of (0.5, 1.0 and 1.5%), under a design completely randomized, with six treatments and four repetitions of 50 seeds. The germination test (PRUGER) was performed in a mesh house with four repetitions of 50 seeds to estimate eight physiological parameters of germination. The morpho-metric characteristics of thickness, length and width of seeds showed reduced phenotypic variation, compared with the weight, density and volume of a seed, with variation coefficients greater than 11.7%. The seed, ovoid and flattened, presents an embryo with its cotyledons wrapped in a protective membrane. The viability, determined with tetrazolium, was 89.5-96.5%, while the PRUGER was 100%, with no statistical differences between them (P≥0.05). The combination of 0.5% tetrazolium with 2 hours is sufficient to determine the viability of the seed. Germination elapsed from 6 to 19 days after sowing, with a germination speed index of 0.22, average daily germination of 5.26%, peak value of 5.33 and germination value of 28.

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

scholarly journals Viability of Carica papaya L. Seeds by the Tetrazolium Test

2018 ◽  
Vol 10 (2) ◽  
pp. 335 ◽  
Author(s):  
Sara M. C. Carvalho ◽  
Salvador B. Torres ◽  
Erivanessa C. Sousa ◽  
Danielle M. M. Sousa ◽  
Kleane T. O. Pereira ◽  
...  
Keyword(s):  
Decision Making ◽  
Carica Papaya ◽  
Seed Viability ◽  
Germination Test ◽  
Long Period ◽  
Physiological Quality ◽  
Rapid Tests ◽  
Papaya Seeds ◽  
Tetrazolium Test

Germination test for papaya seeds demands thirty days to obtain the results, which is a long period for decision-making. Thus, rapid tests become essential tools to evaluate seed viability, and the tetrazolium test is an alternative for this purpose. Hence, this study aimed to establish the adequate procedure to apply this test, in order to reduce the time of evaluation of papaya seeds. For that, four tetrazolium solution concentrations (0.05, 0.1, 0.5 and 1%) and three staining times (3, 6 and 9 hours) were tested at temperatures of 35 and 40 ºC. The experimental design was completely randomized in 4 × 3 + 1 factorial scheme (concentrations × staining times + one control = germination test). It was found that the tetrazolium test allows to evaluate the physiological quality of papaya seeds, thus reducing the time for decision-making. For the test, we recommend using the concentrations of 0.1% for 9 hours or 1% for 6 hours of staining, at temperature of 40 ºC.

scholarly journals Tetrazolium test adjustment for wheat seeds

2013 ◽  
Vol 35 (3) ◽  
pp. 361-367 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Francisco Carlos Krzyzanowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco
Keyword(s):  
Seed Viability ◽  
Longitudinal Section ◽  
Wheat Seed ◽  
Germination Test ◽  
Efficient Procedure ◽  
Paper Towels ◽  
Direct Immersion ◽  
Tetrazolium Test ◽  
Wheat Seeds ◽  
Test Adjustment

The assessment of the germination test in wheat seeds varies from 4 to 15 days, because the species normally presents dormancy in freshly harvested seeds. The tetrazolium test can characterize seed viability in less than 24 hours including lots with dormancy seeds. The objective of this study was to develop a practical and efficient procedure for evaluating the viability of wheat seeds using the tetrazolium test. Five seed lots of the BRS 208 cultivar were used, where the following were tested: a) pre-conditioning between moist paper towels or direct immersion in water for 18 hours, at 20 °C; b) longitudinal section of the embryo and the endosperm; c) coloration on paper or by immersion for 2 and 3 hours, at 30 and 40 °C; and d) concentrations of tetrazolium solution at 0.075%, 0.1%, 0.5% and 1.0%. The tetrazolium test may be efficiently used to evaluate wheat seed viability by pre-conditioning the seeds between paper towels (18 hours, at 20 °C) and adopting the following combinations of preparation and coloration: coloration of both halves of the seed on paper (2 hours, at 30 °C), in a 1.0% tetrazolium solution; or coloration of one half of the seed by immersion (3 hours, at 30 °C), in a 0.1% tetrazolium solution; or coloration of one half of the seed by immersion (2 hours, at 40 °C), in a 0.075% tetrazolium solution. This latter procedure is recommended for identifying and discarding lots with lower viability.

scholarly journals Effect of light quality on Tabebuia rosea seed germination

2014 ◽  
Vol 20 (2) ◽  
pp. 191
Author(s):  
Sofía Basto ◽  
Claudia Ramírez
Keyword(s):  
Seed Germination ◽  
Light Quality ◽  
Germination Rate ◽  
Germination Capacity ◽  
Germination Speed ◽  
Tabebuia Rosea ◽  
Germination Value ◽  
Positive Effect ◽  
Effect Of Light ◽  
Peak Value

Tabebuia rosea germination capacity and rate have been examined under light and dark conditions. However, seeds responses to light quality, in particular to short and mid-wavelengths, are not well known. We hypothesized that short wavelengths would have a positive effect on rosea germination. The effects of short, mid and long-wavelengths on germination capacity, rate (germination rate index, germination rate R50, a devised index of germination rate R50´ and peak value), mean daily germination and germination value were evaluated. Light quality had little effect on germination capacity; it was reduced only in darkness. Seeds under short and mid-wavelengths germinated faster (germination rate) than under any other light treatments. We concluded that althoughlt T. rosea seeds respond to all light quality treatments, the short and mid-wavelengths are the cue for increasing germination speed. For synchronizing germination, short and mid-wavelengths transmitted by red cellophane paper can be used as a pre-germinative treatment for restoration and silvicultural purposes.

scholarly journals Criteria for implementation of a tetrazolium test in canola seeds

2015 ◽  
Vol 37 (4) ◽  
pp. 222-227 ◽  
Author(s):  
Mariana Faber Flores ◽  
Camila Ribeiro de Souza Grzybowski ◽  
Kelly Pazolini ◽  
Jean Carlo Possenti ◽  
Maristela Panobianco
Keyword(s):  
Incubation Period ◽  
Crop Rotation ◽  
Vegetable Oil ◽  
Salt Concentration ◽  
Seed Viability ◽  
Human Consumption ◽  
Tetrazolium Salt ◽  
Canola Seed ◽  
Germination Test ◽  
Tetrazolium Test

Canola is an oilseed winter plant, constituting an alternative to crop rotation. It presents socioeconomic value for the production of vegetable oil for human consumption, biodiesel and animal food formulation. The evaluation of canola seed viability is routinely performed by germination test, which can be extended for up to 14 days if it is necessary to overcome dormancy. The objective of this study was to establish criteria for conducting a tetrazolium test in canola seeds, determining viability groups. Four lots of seed were used, placed between soaked paper to hydrate for 16 hours at 20 °C. In the coloration step, after preliminary tests, the following combinations of tetrazolium salt concentration, temperature and incubation period were tested: 0.5 and 1.0% at 30 °C; 0.2, 0.5 and 1.0% at 40 °C for two, four and six hours. It was concluded that the evaluation of viability of canola seeds by the tetrazolium test is effective upon hydration of the seeds between papers for 16 hours at 20 °C, coloration by immersion in 0.5% tetrazolium solution for six hours, at 40 °C and two viability groups were established and illustrated.

scholarly journals Viability of recently harvested and stored Xylopia aromatica (Lam.) Mart. (Annonaceae) seeds

2012 ◽  
Vol 34 (3) ◽  
pp. 408-415 ◽  
Author(s):  
Fábio Socolowski ◽  
Silvio Moure Cicero ◽  
Daniela Cristine Mascia Vieira
Keyword(s):  
Native Species ◽  
Restoration Ecology ◽  
Seed Viability ◽  
Distilled Water ◽  
Germination Test ◽  
X Ray ◽  
Morphophysiological Dormancy ◽  
Two Temperatures ◽  
Tetrazolium Test

Xylopia aromatica is a native species from Brazil's "Cerrado", recommended for restoration ecology and also as a medicine. Its seeds have embryos with morphophysiological dormancy, making nursery propagation difficult. The objective of this study was to verify the efficiency of X-ray and tetrazolium tests for evaluating the viability of three seed lots, stored for different periods. All seeds were X-rayed (13 kV, 350 seconds) and samples used for tetrazolium and germination tests. In the tetrazolium test, seeds were submitted to six treatments at two temperatures (25 and 30 °C) with imbibition in distilled water and immersion in three concentrations of tetrazolium solution (0.5, 0.75 and 1%) at the two imbibition temperatures. Seeds for the germination test were placed for imbibition in distilled water and a 500 ppm Promalin® (6-Benzyladenine + GA4 + GA7) solution and later sown in sterilized sand. The embryo could not be observed with the X-ray test. However, those seeds observed with an undamaged endosperm did not differ in the percentages of seeds with firm and stained endosperms observed in the tetrazolium test for all the lots. The tetrazolium test is efficient for evaluating seed viability, principally if imbibed at 30 °C and immersed in a 0.5% solution at 30 °C.

scholarly journals Tetrazolium test for assessment of seed viability of atemoya (Annona cherimola Mill. x A. squamosa L.)

2014 ◽  
Vol 36 (3) ◽  
pp. 357-361 ◽  
Author(s):  
Juliana Iassia Gimenez ◽  
Gisela Ferreira ◽  
Claudio Cavariani
Keyword(s):  
Experimental Design ◽  
Seed Viability ◽  
Tetrazolium Salt ◽  
Germination Test ◽  
Tissue Integrity ◽  
Annona Cherimola ◽  
The Family ◽  
Viable Seeds ◽  
Tetrazolium Test

The study aimed to adapt the tetrazolium test to assess the viability of atemoya (Annona cherimola Mill. x A. squamosa L.) seeds. The experimental design was completely randomized in 3 x 3 factorial arrangement (tetrazolium salt concentrations x exposure times), totaling 9 treatments with four replicates of 25 seeds. After immersion in water for 24 hours, the seeds were longitudinally sectioned and exposed to three tetrazolium salt concentrations (0.05%, 0.5% and 1%) for three periods of time (2, 4 and 6 hours). The evaluations were performed according to pre-established criteria of tissue integrity and staining. The germination test was performed, using the GA 4+7 + N-(fenilmetil)-aminopurina regulators at concentrations of 0 and 300 mg.L-1, because dormancy is observed in species of the family Annonaceae. Tetrazolium salt at 1% yielded the highest percentage of viable seeds within the shortest time, while longer exposure times were necessary with concentrations of 0.05% and 0.5%. It can be concluded that the concentration of 1% of tetrazolium salt for two hours of exposure is more appropriate for assessing the viability of atemoya seeds.

Can the Tetrazolium Test be Used as an Alternative to the Germination Test in Determining Seed Viability of Grass Species?

Crop Science ◽  
2016 ◽  
Vol 56 (2) ◽  
pp. 707-715 ◽  
Author(s):  
Vanessa N. Soares ◽  
Sabry G. Elias ◽  
Gizele I. Gadotti ◽  
Adriel E. Garay ◽  
Francisco A. Villela
Keyword(s):  
Seed Viability ◽  
Grass Species ◽  
Germination Test ◽  
Tetrazolium Test

scholarly journals Rapid results of peach palm seed viability: a methodological proposition for the tetrazolium test

2020 ◽  
Vol 42 ◽  
Author(s):  
Andreza Cerioni Belniaki ◽  
Thomas Bruno Michelon ◽  
Elisa Serra Negra Vieira ◽  
Maristela Panobianco
Keyword(s):  
Moisture Content ◽  
Seed Viability ◽  
Bactris Gasipaes ◽  
Germination Test ◽  
Peach Palm ◽  
Half Seed ◽  
Tetrazolium Test

ABSTRACT: Prior to commercialization, seeds of peach palm (Bactris gasipaes Kunth) have to undergo the germination test, whose well-established methodology takes 120 days. Due to their recalcitrant behavior, the seeds have short longevity when stored (around 30-45 days), which makes it challenging to select the most viable ones for marketing. This study aimed to determine a methodology for the tetrazolium test to be carried out in peach palm seeds, in order to fast deliver results that can be correlated to the germination test. Different forms of pre-conditioning, preparation, and staining were investigated via moisture content, germination, and tetrazolium tests, so as to define the vital parts of the seed and sort out the viability classes. For the seed lot under study, the tetrazolium test delivered results supported by the germination test when the following procedures were adopted: pre-conditioning by water submersion (20 °C for 24 h), longitudinal cut adjacent to the embryo, and half-seed immersion (embryo + endosperm) in a 1.0% tetrazolium solution for 4 h at 30 °C. Having fulfilled these criteria, it became possible to separate the peach palm seeds into two classes (viable or non-viable).

scholarly journals TETRAZOLIUM TEST TO ESTIMATE OKRA (Abelmoschus esculentus (L.) MOENCH) SEED VIABILITY

2018 ◽  
Vol 31 (4) ◽  
pp. 817-822 ◽  
Author(s):  
Antônia Adailha Torres Souza ◽  
Narjara Walessa Nogueira ◽  
Salvador Barros Torres ◽  
Clarisse Pereira Benedito ◽  
Maria Lilia de Souza Neta ◽  
...  
Keyword(s):  
Test Procedure ◽  
Seed Viability ◽  
Abelmoschus Esculentus ◽  
Tetrazolium Salt ◽  
Distilled Water ◽  
Germination Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tetrazolium Test

ABSTRACT This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an 18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.

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