scholarly journals TETRAZOLIUM TEST TO ESTIMATE OKRA (Abelmoschus esculentus (L.) MOENCH) SEED VIABILITY

2018 ◽  
Vol 31 (4) ◽  
pp. 817-822 ◽  
Author(s):  
Antônia Adailha Torres Souza ◽  
Narjara Walessa Nogueira ◽  
Salvador Barros Torres ◽  
Clarisse Pereira Benedito ◽  
Maria Lilia de Souza Neta ◽  
...  
Keyword(s):  
Test Procedure ◽  
Seed Viability ◽  
Abelmoschus Esculentus ◽  
Tetrazolium Salt ◽  
Distilled Water ◽  
Germination Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tetrazolium Test

ABSTRACT This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an 18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.

scholarly journals Qualidade Física e Fisiológica de Sementes Comercializadas de Capim-Colonião em Campo Grande - MS

2020 ◽  
Vol 24 (1) ◽  
pp. 07-11
Author(s):  
Silvia Rahe Pereira ◽  
Adriana Paula D’Agostini Contreiras-Rodrigues ◽  
Daiane Rodrigues Oliveira ◽  
Valdemir Antônio Laura ◽  
Daniela Aparecida Dalla-Costa
Keyword(s):  
Cultural Value ◽  
Germination Test ◽  
Tropical Regions ◽  
Genetic Potential ◽  
Randomized Design ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
Maximum Expression ◽  
Tetrazolium Test

O Centro-Oeste brasileiro representa a região brasileira com maior área e produção de sementes de Megathyrsus maximus, uma gramínea muito utilizada para a formação de pastagens em regiões tropicais do Brasil e outros países da América. Objetivou-se no presente estudo diagnosticar a qualidade física e fisiológica de sementes de três cultivares da espécie M. maximus (Mombaça, BRS Tamani e BRS Zuri) comercializadas por diferentes empresas de Campo Grande (MS). Os experimentos foram conduzidos utilizando-se sementes da safra 2017-2018 em um delineamento inteiramente casualizado, em esquema fatorial 3 x 6, avaliando-se três cultivares e seis procedências. As variáveis analisadas foram: pureza física, peso de mil sementes, primeira contagem do teste de germinação, germinação, valor cultural e teste de tetrazólio. Os resultados encontrados demonstram que, com exceção de um lote da cultivar Mombaça, os demais lotes das três cultivares da espécie Megathyrsus maximus (Mombaça, BRS Tamani e BRS Zuri), de todas as procedências avaliadas, apresentaram qualidade física e fisiológica compatível com a comercialização em função da legislação vigente. No entanto, é possível encontrar lotes com qualidade distinta no mercado de Campo Grande. Assim, a escolha adequada da procedência (empresa) vai garantir a expressão máxima do potencial genético, subsidiada por processos adequados de produção e pós-colheita. Palavras-chave: Megathyrsus Mombaça. Gramíneas Tropicais. Teste de Germinação. Teste de Tetrazólio. Valor Cultural.   Abstract Brazilian's Midwest represents the region with the largest area and production of Megathyrsus maximus seeds, a grass widely used for the pastures formation in tropical regions of Brazil and other countries in America.The objective of this study was to diagnose the physical and physiological quality of seeds of three cultivars of the species Megathyrsus maximus (Mombaça, BRS Tamani and BRS Zuri) commercialized by different companies of Campo Grande (MS). The experiments were conducted using seeds of the 2017-2018 harvest, in a completely randomized design, using a factorial scheme 3 x 6, with three species and six sources, totaling 18 treatments. The variables analyzed were: physical purity, weight of one thousand seeds, first count of germination test, germination, cultural value and tetrazolium. The results showed that with the exception of one lot of cultivar Mombaça, the other lots of the three cultivars of species Megathyrsus maximus (Mombasa, BRS Tamani and BRS Zuri), of all provenances, have compatible physical and physiological quality with marketing in accordance with current legislation. However, it is possible to find lots of different quality in the Campo Grande market. Thus, the appropriate choice of source (company) will guarantee the maximum expression of genetic potential, subsidized by adequate production and post-harvest processes. Keywords: Megathyrsus mombaça. Tropical Grasses. Germination Test. Tetrazolium Test. Cultural Value.

scholarly journals Criteria for implementation of a tetrazolium test in canola seeds

2015 ◽  
Vol 37 (4) ◽  
pp. 222-227 ◽  
Author(s):  
Mariana Faber Flores ◽  
Camila Ribeiro de Souza Grzybowski ◽  
Kelly Pazolini ◽  
Jean Carlo Possenti ◽  
Maristela Panobianco
Keyword(s):  
Incubation Period ◽  
Crop Rotation ◽  
Vegetable Oil ◽  
Salt Concentration ◽  
Seed Viability ◽  
Human Consumption ◽  
Tetrazolium Salt ◽  
Canola Seed ◽  
Germination Test ◽  
Tetrazolium Test

Canola is an oilseed winter plant, constituting an alternative to crop rotation. It presents socioeconomic value for the production of vegetable oil for human consumption, biodiesel and animal food formulation. The evaluation of canola seed viability is routinely performed by germination test, which can be extended for up to 14 days if it is necessary to overcome dormancy. The objective of this study was to establish criteria for conducting a tetrazolium test in canola seeds, determining viability groups. Four lots of seed were used, placed between soaked paper to hydrate for 16 hours at 20 °C. In the coloration step, after preliminary tests, the following combinations of tetrazolium salt concentration, temperature and incubation period were tested: 0.5 and 1.0% at 30 °C; 0.2, 0.5 and 1.0% at 40 °C for two, four and six hours. It was concluded that the evaluation of viability of canola seeds by the tetrazolium test is effective upon hydration of the seeds between papers for 16 hours at 20 °C, coloration by immersion in 0.5% tetrazolium solution for six hours, at 40 °C and two viability groups were established and illustrated.

scholarly journals Viability of recently harvested and stored Xylopia aromatica (Lam.) Mart. (Annonaceae) seeds

2012 ◽  
Vol 34 (3) ◽  
pp. 408-415 ◽  
Author(s):  
Fábio Socolowski ◽  
Silvio Moure Cicero ◽  
Daniela Cristine Mascia Vieira
Keyword(s):  
Native Species ◽  
Restoration Ecology ◽  
Seed Viability ◽  
Distilled Water ◽  
Germination Test ◽  
X Ray ◽  
Morphophysiological Dormancy ◽  
Two Temperatures ◽  
Tetrazolium Test

Xylopia aromatica is a native species from Brazil's "Cerrado", recommended for restoration ecology and also as a medicine. Its seeds have embryos with morphophysiological dormancy, making nursery propagation difficult. The objective of this study was to verify the efficiency of X-ray and tetrazolium tests for evaluating the viability of three seed lots, stored for different periods. All seeds were X-rayed (13 kV, 350 seconds) and samples used for tetrazolium and germination tests. In the tetrazolium test, seeds were submitted to six treatments at two temperatures (25 and 30 °C) with imbibition in distilled water and immersion in three concentrations of tetrazolium solution (0.5, 0.75 and 1%) at the two imbibition temperatures. Seeds for the germination test were placed for imbibition in distilled water and a 500 ppm Promalin® (6-Benzyladenine + GA4 + GA7) solution and later sown in sterilized sand. The embryo could not be observed with the X-ray test. However, those seeds observed with an undamaged endosperm did not differ in the percentages of seeds with firm and stained endosperms observed in the tetrazolium test for all the lots. The tetrazolium test is efficient for evaluating seed viability, principally if imbibed at 30 °C and immersed in a 0.5% solution at 30 °C.

scholarly journals Methodological adjustments to the tetrazolium test in coriander seeds

2021 ◽  
Vol 43 ◽  
Author(s):  
Raimunda Nonata Oliveira da Silva ◽  
Gizele Ingrid Gadotti ◽  
Ireni Leitzke Carvalho ◽  
Ivan Ricardo Carvalho ◽  
Jerffeson Araujo Cavalcante ◽  
...  
Keyword(s):  
Decision Making ◽  
Water Content ◽  
Salt Solution ◽  
Staining Pattern ◽  
Tetrazolium Salt ◽  
Post Harvest ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Direct Immersion ◽  
Tetrazolium Test

Abstract: The reduction in the time to perform the tetrazolium test is critical in decision making at pre and post-harvest of seeds. Thus, the objective of this study was to investigate different concentrations of the tetrazolium salt solution, temperatures, and staining times, to reduce the time for assessing the viability of coriander seeds by the tetrazolium test. The study was divided into two experiments conducted independently but sequentially. The seeds were evaluated for water content, germination, and viability in both experiments by the tetrazolium test. A completely randomized design was used, with four replications. For the tetrazolium test, the seeds were preconditioned by direct immersion in water for sixteen hours at 20 °C. In experiment I, the seeds were exposed to different concentrations of the tetrazolium salt solution (0.25; 0.5; 0.75; and 1.0%). In experiment II, different combinations of temperatures (35; 40; and 45 °C) and staining times (three, four, five, and six hours) were tested. Based on the findings, it was concluded that the best staining pattern of coriander seeds for performing the tetrazolium test is achieved by using the concentration of 0.5% tetrazolium salt solution for five hours at 40 °C.

scholarly journals Tetrazolium test for assessment of seed viability of atemoya (Annona cherimola Mill. x A. squamosa L.)

2014 ◽  
Vol 36 (3) ◽  
pp. 357-361 ◽  
Author(s):  
Juliana Iassia Gimenez ◽  
Gisela Ferreira ◽  
Claudio Cavariani
Keyword(s):  
Experimental Design ◽  
Seed Viability ◽  
Tetrazolium Salt ◽  
Germination Test ◽  
Tissue Integrity ◽  
Annona Cherimola ◽  
The Family ◽  
Viable Seeds ◽  
Tetrazolium Test

The study aimed to adapt the tetrazolium test to assess the viability of atemoya (Annona cherimola Mill. x A. squamosa L.) seeds. The experimental design was completely randomized in 3 x 3 factorial arrangement (tetrazolium salt concentrations x exposure times), totaling 9 treatments with four replicates of 25 seeds. After immersion in water for 24 hours, the seeds were longitudinally sectioned and exposed to three tetrazolium salt concentrations (0.05%, 0.5% and 1%) for three periods of time (2, 4 and 6 hours). The evaluations were performed according to pre-established criteria of tissue integrity and staining. The germination test was performed, using the GA 4+7 + N-(fenilmetil)-aminopurina regulators at concentrations of 0 and 300 mg.L-1, because dormancy is observed in species of the family Annonaceae. Tetrazolium salt at 1% yielded the highest percentage of viable seeds within the shortest time, while longer exposure times were necessary with concentrations of 0.05% and 0.5%. It can be concluded that the concentration of 1% of tetrazolium salt for two hours of exposure is more appropriate for assessing the viability of atemoya seeds.

scholarly journals Criopreservação de sementes de rainha do abismo (Sinningia leucotricha)

2017 ◽  
Vol 23 (1) ◽  
pp. 15 ◽  
Author(s):  
Vanessa Stegani ◽  
Guilherme Augusto Cito Alves ◽  
Douglas Junior Bertoncelli ◽  
Ricardo Tadeu de Faria
Keyword(s):  
Liquid Nitrogen ◽  
Dry Mass ◽  
Germination Percentage ◽  
Distilled Water ◽  
Germination Test ◽  
Blotting Paper ◽  
Cryogenic Solutions ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Cryoprotective Solution

The objective was to evaluate the use of cryogenic solutions in cryopreservation Sinningia leucotricha seeds by the vitrification method in liquid nitrogen. The treatments were: T1 - control: without cryoprotective solution; T2 - PVS1; T3 - modified PVS1; T4 - PVS2; T5 - modified PVS2; T6 - PVS3; T7 - PVS3 modified; T8 - PVS2 + 1% phloroglucinol. After 15 days of immersion of seeds in LN, the cryotubes were removed and rapidly reheated to a temperature of 40 °C water bath for 1.5 minutes. Then, the seeds were washed with wash solution for 20 min. Later they were submitted to the germination test which was conducted on blotting paper moistened with distilled water packaged in crystal polystyrene boxes kept in a growth chamber at 25 ± 2 °C and 16 hours photoperiod. Was evaluated the germination percentage the germination speed index (GSI), and at the end of the experiment will determine the length of shoot (LS) and root (LR), and dry mass of seedlings (DMS). We used a completely randomized design with eight treatments and five replications, consisting of 100 seeds. The direct submission of the queen of the abyss seeds in liquid nitrogen provided the highest germination values, GSI, LS, LR and DMS. The queen of the abyss seeds can be cryopreserved in liquid nitrogen directly without the need to cryoprotectant solutions.

scholarly journals Suitability of tetrazolium test for Tamarindus indica L. seeds

2022 ◽  
Vol 43 (2) ◽  
pp. 509-522
Author(s):  
Luciana Nunes Cordeiro ◽  
◽  
Riselane de Lucena Alcântara Bruno ◽  
Lucas Kennedy Silva Lima ◽  
Albericio Pereira de Andrade ◽  
...  
Keyword(s):  
Seed Quality ◽  
Seed Viability ◽  
Dry Weight ◽  
Tetrazolium Salt ◽  
Tamarindus Indica ◽  
Test Protocol ◽  
Randomized Design ◽  
Class 1 ◽  
Set Up ◽  
Tetrazolium Test

Seed quality is routinely assessed by direct tests, e.g, the germination test, or indirect tests like the tetrazolium test, which has shoown to be promising in the determine viability and vigor, allowing the diagnosis of the main problems that may affect seed quality, such as mechanic damages, field deterioration and storage. In this respect, this study was conducted to develop a tetrazolium test protocol to evaluate the viability and vigor of Tamarindus indica L. seeds. Before exposing the seeds to the tetrazolium solution, seed preconditioning studies were carried out in which seven soaking times were tested. The soaking time that did not cause damage to the seed embryo and allowed the removal of the seed coat to expose the seed structures to the tetrazolium salt was selected. Then, an experiment was set up in a completely randomized design with a 2x3x3 factorial arrangement involving two seed lots, three soaking times in tetrazolium salt (6, 12 and 16 h) and three salt concentrations (0.075, 0.1 and 0.5%), totaling 18 treatments with four replicates of 25 seeds, evaluated at 40 ºC. For each treatment, the seeds were divided into three classes, namely, viable and vigorous embryos (class 1); viable embryos (class 2) and non-viable or dead embryos (class 3). For a comparison with the tetrazolium test results, the two seed lots were analyzed for water content, germination, emergence, first count, germination speed index, emergence speed index, growth and seedling dry weight. The viability and vigor of T. indica seeds can be evaluated after a soaking period of 48 h and immersion for 6h in tetrazolium salt at the concentration of 0.1%, at 40°C, with provides results similar to conventional seed viability tests. The tetrazolium test proved to be adequate to differentiate T. indica seed lots in terms of viability.

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

scholarly journals Effect Of Seed Hydration-Dehydration Integrated With Rhizobacteria On Viability And Vigor Of Deteriorated Soybean Seed

2018 ◽  
Vol 1 (1) ◽  
pp. 25-31
Author(s):  
P.K. Dewi Hayati
Keyword(s):  
Seed Viability ◽  
Soybean Seed ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Time Data ◽  
Randomized Design ◽  
Vigor Index ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Hydration And Dehydration

Soybeans undergo rapid deterioration due to its chemical composition and unfavourable storage conditions. The objective of the research was to determine the length period of seed hidration and dehydration which is integrated with rhizobateri to viability and vigor of deteriorated soybean seed.  A completely randomized design with four replicates were used in this experiment. Seeds were treated with varied combination of hydration period in a rhizobacteria suspension and followed by dehydration time.  Data were analysed using the F-test and significant differences were further tested with Least Significant Difference at the 5% level. Results showed that the hydration and dehydration period of seeds in a rhizobacteria suspension improved viability and vigor of seed which have 59.5% initial germination percentage. The improvement was 12.28%, 0.56, 25.4% and 1.4 days for standard germination test, vigor index, first count test percentage and T50, respectively. The hydration for 60 minutes followed by dehydration for 60 minutes gave the best results on seed viability and vigor.

scholarly journals Viability of Carica papaya L. Seeds by the Tetrazolium Test

2018 ◽  
Vol 10 (2) ◽  
pp. 335 ◽  
Author(s):  
Sara M. C. Carvalho ◽  
Salvador B. Torres ◽  
Erivanessa C. Sousa ◽  
Danielle M. M. Sousa ◽  
Kleane T. O. Pereira ◽  
...  
Keyword(s):  
Decision Making ◽  
Carica Papaya ◽  
Seed Viability ◽  
Germination Test ◽  
Long Period ◽  
Physiological Quality ◽  
Rapid Tests ◽  
Papaya Seeds ◽  
Tetrazolium Test

Germination test for papaya seeds demands thirty days to obtain the results, which is a long period for decision-making. Thus, rapid tests become essential tools to evaluate seed viability, and the tetrazolium test is an alternative for this purpose. Hence, this study aimed to establish the adequate procedure to apply this test, in order to reduce the time of evaluation of papaya seeds. For that, four tetrazolium solution concentrations (0.05, 0.1, 0.5 and 1%) and three staining times (3, 6 and 9 hours) were tested at temperatures of 35 and 40 ºC. The experimental design was completely randomized in 4 × 3 + 1 factorial scheme (concentrations × staining times + one control = germination test). It was found that the tetrazolium test allows to evaluate the physiological quality of papaya seeds, thus reducing the time for decision-making. For the test, we recommend using the concentrations of 0.1% for 9 hours or 1% for 6 hours of staining, at temperature of 40 ºC.

Sign in / Sign up

Export Citation Format

Share Document