scholarly journals Environmental exposure to endocrine disruptors on semen quality of men

2018 ◽  
Vol 19 (3) ◽  
pp. 42-47
Author(s):  
S. V. Chigrinets ◽  
G. V. Bryuhin
Keyword(s):  
Bisphenol A ◽  
Dna Fragmentation ◽  
Endocrine Disruptors ◽  
Semen Quality ◽  
World Health ◽  
Plasma Testosterone ◽  
Total Testosterone ◽  
Study Objective ◽  
Normal Morphology

The study objective is to investigate the correlations between endocrine disruptors (bisphenol A, triclosan and 4-nonylphenol) exposure, measured on semen and semen quality of men, total testosterone in the plasma.Materials and methods. We measured semen bisphenol A, triclosan and 4-nonylphenol concentrations of 41 men by gas chromatography/ mass spectrometry. According to the World Health Organization guidelines, we divided our patients into 2 groups: normozoospermia and patozoospermia. And furthermore, total plasma testosterone and index DNA fragmentation spermatozoa were determined. The results were statistically processed using the Mann–Whitney U-test and correlation analysis. A p-value less than 0.05 was considered significant.Results. Bisphenol A was detected in 100 % of semen samples, triclosan – in 85,4 %, 4-nonylphenol – in 97,6 %, with a median concentration of 0.108, 0.17 and 0.165 ng/ml respectively. The groups were different statistically significant for levels of bisphenol A, triclosan and index DNA fragmentation spermatozoa (р <0.001, р = 0.033, р = 0.005 respectively). We found significant negative correlations between concentration of triclosan and percentage of normal morphology (p = 0.004), percentage of progressively motile spermatozoa (p = 0.049) and the positive correlation – between concentration of triclosan and percentage of DNA fragmentation spermatozoa (p <0,001). Аlso we found significant negative correlations between concentration of bisphenol A and total count (p = 0.04), percentage of progressively motile spermatozoa (p <0.001), percentage of normal morphology (p = 0.002), levels of total testosterone in plasma (p = 0.047). We demonstrated the synergy of investigated endocrine disruptors on semen quality.Conclusion. Semen bisphenol A and triclosan concentrations have a negative correlation with the parameters of the ejaculate. Environmental levels of bisphenol A negatively correlated with testosterone levels in plasma.

scholarly journals Environmental Exposure to Endocrine Disruptor of Bisphenol A and Semen Quality of Men

2018 ◽  
Vol 73 (5) ◽  
pp. 338-343
Author(s):  
Stanislav V. Chigrinets ◽  
Gennady V. Brukhin
Keyword(s):  
Reproductive Health ◽  
Male Infertility ◽  
Bisphenol A ◽  
Seminal Fluid ◽  
Concentration Level ◽  
Semen Quality ◽  
Roc Curves ◽  
Total Testosterone ◽  
Idiopathic Male Infertility

Background: The reproductive health disorders in men are one of the urgent problems of international medicine. The prevalence of idiopathic male infertility has the highest rate. Oxidative stress, genetic factor, and endocrine disruptors are considered to be the most probable causes for the idiopathic male infertility. In this regard, studying the effect of endocrine disruptors, in particular bisphenol A on male reproductive health, becomes actual and relevant. Aims: The aim of the study was to reveal the relationship between the concentration level of bisphenol A (BPA) in seminal fluid and semen quality in men with normo- or pathozoospermia, as well as between the concentration level of bisphenol A and the level of total testosterone and estradiol in plasma. Materials and methods: 53 samples of seminal fluid of men with normo- or pathoospermia were studied. In seminal fluid the concentration of bisphenol A was determined by gas chromatography with mass spectrometry (GC-MS). The spermiological analysis was performed according to the WHO recommendations (2010) including the evaluation of sperm count/concentration, motility and morphology, and DNA fragmentation index. In addition, the concentration of total testosterone and estradiol in plasma was determined. The results were statistically processed using the Mann–Whitney U test, the correlation analysis, the paired regression method, and the ROC curves to determine the cut-off point for BPA in the seminal fluid. The results were considered statistically significant at p0.05. Results: In 100% of the ejaculate samples BPA with a median concentration of 0.15 (0.06–0.31) ng/ml was detected. Using the Spearman rank correlation coefficient, statistically significant correlations between the concentration of BPA and the total count (r=-0.330, p=0.016), concentration (r=-0.309; p=0.024), the proportion of progressively motile spermatozoa (r=-0.575; p=0.001), the proportion of spermatozoa with normal morphology (r=-0.397, p=0.003), the degree of sperm DNA fragmentation (r=0.349, p=0.025), and the concentration of total testosterone (r =-0.616; p0.001) were registered. A statistically significant inverse linear relationship (r=-0.406, p=0.003) and (r =-0.364, p=0.048) was determined by a paired linear regression between the BPA concentration in the seminal fluid and the proportion of progressively motile spermatozoa, and the total testosterone level respectively. To assess the risk of pathozoospermia, the threshold value of seminal BPA concentration was determined using the analysis of ROC-curves; the cut-off point was 0.1025 ng/ml. Conclusions: BPA in the seminal fluid influences negatively on the quality of the sperm and suppress the level of total testosterone in plasma.

scholarly journals Connection of urethral microbiota with the semen quality and the concentration of endocrine disrupters in men semen

2018 ◽  
Vol 19 (4) ◽  
pp. 60-66
Author(s):  
S. V. Chigrinets ◽  
G. V. Bryuhin
Keyword(s):  
Bisphenol A ◽  
Seminal Fluid ◽  
Semen Quality ◽  
Statistical Processing ◽  
Test System ◽  
P Value ◽  
Study Objective ◽  
Comparison Groups

The study objective is to investigate the correlations between opportunistic microorganisms of the urethra and the quality of ejaculate, as well as the concentration of ubiquitory endocrine disruptors (bisphenol A and triclosan) in men semen.Materials and methods. We analysed 63 semen samples of men with normo- and patozoospermia. In seminal fluid the concentration of bisphenol A and triclosan was determined by gas chromatography with mass spectrometry. The microbiocenosis of the urethra was studied by real-time polymerase chain reaction using the Androflor test system. The results were subjected to statistical processing using the Mann — Whitney U-test and correlation analysis. A p-value less than 0.05 was considered significant.Results. Bisphenol A was found in 100 % of the semen samples. Triclosan was detected in 84.9 % of the samples. Comparison groups on the quality of ejaculate (normo- and pathozoospermia) were statistically significantly different in the concentration of bisphenol A and triclosan in seminal fluid (p <0.001) and (p = 0.003) respectively. The study established statistically significant correlations between bisphenol A concentration, seminal triclosan and urethral microbiota: Lactobacillus spp. (r = 0.501, p = 0.003), Corynebacterium spp. (r = 0.425, p = 0.015), Anaerococcus spp. (r = 0.371, p = 0.045), Eubacterium spp. (r = 0.357, p = 0.037) for bisphenol A and Staphylococrns spp. (r = 0.392, p = 0.026), Streptococcus spp. (r = —0.486, p = 0.005) for triclosan.Conclusion. In this study the connection of the urethral microbiota with the quality of the ejaculate and the concentration of endocrine dis-ruptors (bisphenol A and triclosan) in men semen was established. In this regard, it is necessary to take into account the role of asymptomatic urethral dysbiosis when administering patients with poor ejaculate quality.

Quality of ram spermatozoa separated with modified swim up method

2018 ◽  
Vol 33 (2) ◽  
pp. 62-70 ◽  
Author(s):  
A Hossain ◽  
MM Islam ◽  
F Naznin ◽  
RN Ferdousi ◽  
FY Bari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Fluid Medium ◽  
Normal Morphology ◽  
The Mean ◽  
Mass Activity ◽  
Fresh Semen ◽  
Artificial Vagina

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70

scholarly journals Spectrophotometric Analysis of the Resazurin Reduction Test as a Tool for Assessing Canine Semen Quality

2013 ◽  
Vol 57 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Rafał Strzeżek ◽  
Krystyna Filipowicz ◽  
Marta Stańczak ◽  
Władysław Kordan
Keyword(s):  
Sperm Motility ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Spectrophotometric Analysis ◽  
Normal Morphology ◽  
Additional Tool ◽  
Reduction Test ◽  
Highly Correlated

Abstract The resazurin reduction test (RRT) was subjected to spectrophotometric analysis to evaluate the quality of canine semen. Twenty four samples of canine semen were analysed. The absorption peaks for resazurin and resorufin were determined at 615 and 580 nm, respectively. The RRT ratio (RRTsperm-the ratio for samples containing spermatozoa, RRTplasma-the ratio for samples containing seminal plasma) was calculated by dividing the absorbance at 580 nm by the absorbance at 615 nm. Spearman’s correlation test was used to determine the significance of correlations between the analysed sperm parameters and the results of the resazurin reduction assay. The RRT ratio was highly correlated with sperm motility (r=0.68, P<0.01), progressive sperm motility (r=0.61, P<0.01), the subpopulation of cells with rapid velocity (r=0.72, P<0.01), and the subpopulation of cells with medium velocity (r= -0.54, P<0.05). A negative correlation was observed between the reducing capacity of seminal plasma vs. sperm with plasma membrane integrity (r= -0.60, P<0.01) and sperm with normal morphology (r= -0.58, P<0.01). The RRT test can be used as an additional tool for evaluation of the quality of canine semen.

scholarly journals Bacteriospermia and Sperm Quality of Cryopreserved Bull Semen Used in Artificial Insemination of Cows in South Wollo Zone, Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Abadi Amare Reda ◽  
Gizat Almaw ◽  
Solomon Abreha ◽  
Wedajo Tadeg ◽  
Belege Tadesse
Keyword(s):  
Bacterial Contamination ◽  
Semen Quality ◽  
Sperm Quality ◽  
Bacterial Load ◽  
Bacterial Count ◽  
Normal Morphology ◽  
Bull Semen ◽  
Significant Difference ◽  
Processing And Storage

The objectives of this trial were to estimate prevalence of bacteriospermia, to determine the bacterial load, and to isolate the types of bacteria as well as to assess the association between bacterial load and sperm quality traits in cryopreserved bull semen in field conditions in the South Wollo Zone. A total of 309 cryopreserved straws of semen from the Holstein Friesian (HF)-cross bull (n = 180 straws) and pure Jersey bull (n = 129 straws) were investigated. Bacteriological assessments of the presence of aerobic bacteria, estimation of bacterial count and bacterial isolation, as well as semen quality were performed. Aerobic bacterial contamination was prevalent in 38.8% of the semen straws. No significant difference in the prevalence of bacteriospermia was observed among bulls although the HF-cross bull had a higher prevalence (40.0%). But, significant difference in prevalence of bacteriospermia was found among semen ejaculates of the same bull. The risk of bacteriospermia in the HF-cross bull was higher (Odds ratio = 1.86, 95% CI = 0.168–20.26) compared to Jersey although not significant. Overall average bacterial load of 50.38 ± 16.29 colony-forming units (CFU)/ml (from nil to 1318.20 CFU/ml) was found. No significant difference in bacterial count among bulls and their ejaculates was observed. Moreover, correlation analysis revealed that the proportions of motility, live, and normal morphology were negatively influenced by an increase in the bacterial contamination of semen. In this study, three isolates of coagualse-negative Staphylococcus species and one isolate of Corynebacterium species were found. Average percentages of sperm motility (48.35 ± 1.23), live (66.08 ± 1.0), and normal morphology (80.62 ± 1.24) were observed. It was concluded that cryopreservation does not guarantee the quality of semen from bacterial contamination. Hence, meticulous care should be adopted to prevent contamination of semen by bacteria during collection, transportation, processing, and storage times.

105 Increasing fertility of interspecific hybrid males using biotechnological approaches

2021 ◽  
Vol 33 (2) ◽  
pp. 159
Author(s):  
A. Vetokh ◽  
A. Tadzhieva ◽  
B. Iolchiev ◽  
N. Volkova ◽  
V. Bagirov
Keyword(s):  
Dna Fragmentation ◽  
Interspecific Hybrid ◽  
Semen Quality ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Differential Staining ◽  
Computer Assisted ◽  
Sperm Dna Fragmentation ◽  
Russian Science

The results of AI depend on many factors, with the quality of semen being one of the most important. Not all male hybrids can meet the requirements for semen quality, because they often have reduced fertility following cryopreservation. Thus, it is necessary to improve semen processing before use in AI. The aim of the study was to evaluate the effectiveness of using the “swim-up” flotation method to improve sperm quality of hybrid males of the Ovis genus. Semen from interspecific hybrid rams (1/4 Argali×3/4 Romanov, n=15; 1/8 Argali×7/8 Romanov, n=15) was freshly obtained, frozen–thawed, and processed by the swim-up method. Evaluation of sperm motility was determined using computer-assisted semen analysis. Statistical analysis was performed using SPSS vs.15.0 (ANOVA and t-test; SPSS Inc.). Semen was collected during the breeding season (October–December) via artificial vagina. Assessment of acrosome integrity was determined using differential staining with a Diachem diff-quick kit (NPF ABRIS+). The degree of sperm DNA fragmentation was determined using the acridine-orange test. The sperm freezing/thawing cycle was accompanied by sperm damage and an increase in the proportion of immobile sperm from 10 to 58%, with non-progressive movement increasing from 9 to 19.3%. The number of spermatozoa with abnormal morphology doubled, and the DNA fragmentation index increased from 16 to 26%. Use of the swim-up procedure allowed us to sort progressively motile spermatozoa. The content of progressively motile spermatozoa in the samples obtained from the supernatant was 86%, which was 2.3 times higher than in frozen–thawed sperm (P≤0,01). The obtained results show the effective use of the swim-up procedure to determine the quality of semen in hybrid rams. These studies were carried out with financial support from the Russian Science Foundation, grant No. 18-16-00079 and the Ministry of Science and Higher Education of the Russian Federation.

scholarly journals Effect of preservation time on the quality of frozen semen in indigenous rams

2015 ◽  
Vol 44 (1) ◽  
pp. 10-15 ◽  
Author(s):  
BBA Mahmuda ◽  
Azizun Nesa ◽  
BF Zohara ◽  
MGS Alam ◽  
FY Bari
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Volume Density ◽  
Normal Morphology ◽  
Mean Values ◽  
Frozen Semen ◽  
Significant Difference ◽  
The Mean ◽  
Fresh Semen

The study was carried out to observe the effects of preservation time on the quality of frozen semen of indigenous rams. Semen was collected using AV once a week from 4 rams. Tris based with 10% egg yolk and 7% glycerol extender was used to extend and freezing the semen. Fresh semen was evaluated for volume, density, mass motility and concentration, and mean values were observed as 0.8±0.2ml, 3.0±0.3, 3.2±0.7, 3.9±0.7×109/ml, respectively. Significant difference (p<0.05) was found for all the parameters among the rams. Mean values of motility, viability and normal morphology percentages were 83.3±4.3%, 88.2±4.4%, 84.2±3.5% in fresh semen while those of chilled semen at 40C were 74.7±2.3, 78.8±4.9 and 79.2±2.9%, respectively. For all the parameters, significant (p<0.05) difference was found among the rams. Frozen sperm motility was observed after thawing at 39-400C for 14-15 seconds. The mean motility, viability and normal morphology percentages after freezing for 24hrs, 7, 15 and 30 days of duration were 39.8±3.1, 41.1±4.3, 40.1±4.1 and 39.4±2.9%; 44.5±2.5, 45.3±2.8, 44.6±2.8 and 43.9±2.8%; 71.0±2.0, 71.7±1.5, 70.7±1.7 and 70.3±1.8%, respectively and values did not decrease significantly (p>0.05) with the increasing time of preservation. Non significantly decrease of the semen quality with advance of preservation time indicates the suitability of the protocol used for freezing of indigenous ram semen in Bangladesh.DOI: http://dx.doi.org/10.3329/bjas.v44i1.23113            Bang. J. Anim. Sci. 2014. 44 (1): 10-15

scholarly journals Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program

2020 ◽  
Vol 13 (12) ◽  
pp. 2649-2657
Author(s):  
Berlin Pandapotan Pardede ◽  
Muhammad Agil ◽  
Yudi Yudi ◽  
Iman Supriatna
Keyword(s):  
Dna Fragmentation ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Fertility Rate ◽  
Breeding Program ◽  
Sperm Viability ◽  
Production Site ◽  
Frozen Semen ◽  
Acrosome Integrity

Background and Aim: Various factors can reduce the quality of semen used for artificial insemination and have an impact on fertility decline, such as poor handling during frozen semen distribution. This study was aimed at assessing the quality of frozen-thawed semen after distribution in the field and its importance in maintaining fertility. Materials and Methods: The Brahman Cross (BX) breeding program of PT Lembu Jantan Perkasa, Indonesia, was used. This program was preferred due to its adherence to guidelines that limit the effects of extraneous factors that may affect semen quality. Frozen-thawed semen samples from eight bulls with the same production code were analyzed and compared between the production site (artificial insemination [AI] center) and the field (BX breeding program). Total and progressive motility (PM) of sperm were determined using computer-assisted semen analysis. Plasma membrane integrity (PMI) was assessed using hypoosmotic swelling test, sperm viability using Eosin-Nigrosin staining, acrosome integrity using trypan blue-Giemsa staining, morphological abnormalities using William staining, and DNA fragmentation using toluidine blue staining. The fertility rate was determined using the conception rate (%) derived from AI data based on 502 AI services and 478 cows in the BX breeding program. A t-test was used to compare the quality of frozen-thawed semen before and after distribution. The relationship between the qualities of frozen semen after distribution in the field with fertility was analyzed using Pearson correlation. Results: There was no significant difference (p>0.05) in the quality of frozen-thawed semen (sperm motility, PMI, viability, acrosome integrity, abnormalities, and DNA fragmentation) between the production site (AI center) and after distribution in the field (BX breeding program). The semen met the minimum standards for AI programs. Total motility (r=0.986), PM (r=0.961), sperm viability (r=0.971), PMI (r=0.986), and acrosome integrity (r=0.992) were all positively correlated (p<0.05) with fertility rate; while sperm abnormalities (r=-0.996) and sperm DNA fragmentation (r=0.975) were negatively correlated (p<0.05) with fertility rate. Conclusion: The study showed that to achieve the maximal and optimal fertility rate in bulls in an AI program, the overall quality of frozen-thawed semen in all aspects is critical. This can be achieved if the handling during distribution and storage, as well as the various factors that may affect the quality of semen in the field, can be controlled properly.

269 EFFECT OF DIFFERENT COLLECTION FREQUENCY INTERVALS ON THE QUALITY OF SEMEN OF FRENCH BULLDOGS

2015 ◽  
Vol 27 (1) ◽  
pp. 223
Author(s):  
C. B. Deorce ◽  
F. L. G. Leite ◽  
B. Loureiro
Keyword(s):  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Repeated Measure ◽  
Normal Morphology ◽  
The Third ◽  
Counting Chamber ◽  
Increased Sensitivity ◽  
Digital Manipulation

Dogs produce fewer sperm than other species. Furthermore, for French Bulldogs, anatomical peculiarities, low libido, and increased sensitivity to stress could cause further reductions in sperm count. The objective was to compare effects of semen collection at 24- versus 48-h intervals on semen quality of French Bulldogs. Five purebred French Bulldogs, 19 to 48 months old, were subjected to 5 semen collections (24 h apart). After a 30-day rest, collections were repeated, but the interval between collections was 48 h. Semen was collected (all 3 fractions) by digital manipulation without female stimuli. Volume was measured in a 20-mL syringe. Sperm concentration was determined with a Neubauer counting chamber. Motility and vigor were evaluated with a coverslipped drop of semen on a slide (preheated to 37°C). Motility was expressed as a percentage of motile sperm, and vigor was classified on a scale of 1 to 5. Morphology was evaluated by the panoptic method; 100 cells were counted and results expressed as the percentage of normal or defective cells. Effects of collection interval were analysed using PROC MIXED of SAS (animal as subject and collection as a repeated measure), with collections 2 to 5 compared with collection 1 (using the DIFF option). For collection every 24 h, the third, fourth, and fifth sperm collections were lower than the first collection for volume (10.4 ± 1.1, 8.3 ± 1.1, 5.6 ± 1.1, 3.5 ± 1.1, 2.4 ± 1.1 mL), concentration (437 ± 24, 448 ± 24, 370 ± 24, 322 ± 27, 258 ± 31 ×106 sperm mL–1), vigor (4.6 ± 0.2, 4.2 ± 0.2, 3.6 ± 0.2, 3.7 ± 0.2, 3 ± 0.2), and morphologically normal sperm (82 ± 2.2, 83 ± 2.2, 72 ± 2.2, 68 ± 2.5, 66 ± 2.9%). However, when the interval was increased to 48 h, only the fourth and fifth collections were lower (P < 0.05) than the first for volume (11.8 ± 1.1, 10.2 ± 1.1, 8.8 ± 1.1, 6.6 ± 1.1, 2.5 ± 1.1 mL), concentration (447 ± 24, 410 ± 24, 407 ± 24, 322 ± 24, 241 ± 31 ×106 sperm mL–1), vigor (5 ± 0.2, 4.8 ± 0.2, 4.4 ± 0.2, 4.2 ± 0.2, 4 ± 0.2), and sperm with normal morphology (92 ± 2.2, 90 ± 2.2, 87 ± 2.2, 80 ± 2.2, 81 ± 2.9%). Motility decreased (P < 0.05) following the fourth collection at 24-h intervals and decreased (P < 0.05) after the third collection at 48-h intervals. With a 24-h interval, 4 dogs had <60% motility lower at the fifth collection, whereas only 2 dogs had motility <60% on the fifth collection at 48-h intervals. In conclusion, semen collected at 48-h intervals was of better quality than semen collected at 24-h intervals.

Sign in / Sign up

Export Citation Format

Share Document