scholarly journals Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program

2020 ◽  
Vol 13 (12) ◽  
pp. 2649-2657
Author(s):  
Berlin Pandapotan Pardede ◽  
Muhammad Agil ◽  
Yudi Yudi ◽  
Iman Supriatna
Keyword(s):  
Dna Fragmentation ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Fertility Rate ◽  
Breeding Program ◽  
Sperm Viability ◽  
Production Site ◽  
Frozen Semen ◽  
Acrosome Integrity

Background and Aim: Various factors can reduce the quality of semen used for artificial insemination and have an impact on fertility decline, such as poor handling during frozen semen distribution. This study was aimed at assessing the quality of frozen-thawed semen after distribution in the field and its importance in maintaining fertility. Materials and Methods: The Brahman Cross (BX) breeding program of PT Lembu Jantan Perkasa, Indonesia, was used. This program was preferred due to its adherence to guidelines that limit the effects of extraneous factors that may affect semen quality. Frozen-thawed semen samples from eight bulls with the same production code were analyzed and compared between the production site (artificial insemination [AI] center) and the field (BX breeding program). Total and progressive motility (PM) of sperm were determined using computer-assisted semen analysis. Plasma membrane integrity (PMI) was assessed using hypoosmotic swelling test, sperm viability using Eosin-Nigrosin staining, acrosome integrity using trypan blue-Giemsa staining, morphological abnormalities using William staining, and DNA fragmentation using toluidine blue staining. The fertility rate was determined using the conception rate (%) derived from AI data based on 502 AI services and 478 cows in the BX breeding program. A t-test was used to compare the quality of frozen-thawed semen before and after distribution. The relationship between the qualities of frozen semen after distribution in the field with fertility was analyzed using Pearson correlation. Results: There was no significant difference (p>0.05) in the quality of frozen-thawed semen (sperm motility, PMI, viability, acrosome integrity, abnormalities, and DNA fragmentation) between the production site (AI center) and after distribution in the field (BX breeding program). The semen met the minimum standards for AI programs. Total motility (r=0.986), PM (r=0.961), sperm viability (r=0.971), PMI (r=0.986), and acrosome integrity (r=0.992) were all positively correlated (p<0.05) with fertility rate; while sperm abnormalities (r=-0.996) and sperm DNA fragmentation (r=0.975) were negatively correlated (p<0.05) with fertility rate. Conclusion: The study showed that to achieve the maximal and optimal fertility rate in bulls in an AI program, the overall quality of frozen-thawed semen in all aspects is critical. This can be achieved if the handling during distribution and storage, as well as the various factors that may affect the quality of semen in the field, can be controlled properly.

scholarly journals Decreased bull fertility: age-related changes in sperm motility and DNA fragmentation

2020 ◽  
Vol 151 ◽  
pp. 01010
Author(s):  
Berlin P. Pardede ◽  
Iman Supriatna ◽  
Yudi Yudi ◽  
Muhammad Agil
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Fertility Rate ◽  
Blue Staining ◽  
Age Group ◽  
Progressive Motility ◽  
Frozen Semen ◽  
Group 3 ◽  
Group 2

This study aimed to analyze the effect of the age of bulls on sperm motility and DNA fragmentation and its impact on fertility. Ninety-six frozen semen straw from eight bulls were divided into four groups based on age (group-1: 5-6 years; group-2: 7-8 years; group-3: 9-10 years; group-4: 11-12 years). Total and progressive motility were detected by using computer-assisted semen analysis (CASA), while DNA fragmentation was detected by Toluidine blue staining. Over 500 artificial insemination services in the field were used for fertility rate analysis. The results of the analysis of total motility, progressive, and DNA fragmentation in all age groups still meet the minimum standard for artificial insemination programs. Analysis of progressive motility and DNA fragmentation showed significant differences in each age group (P<0.01), whereas analysis of total motility showed no significant differences in group-2 (7-8 years) and group-3 (9-10 years) (P>0.01). Increased age in bulls correlated significantly with increased sperm DNA fragmentation (P<0.01), decreased total and progressive motility (P<0,01), and potentially reduced the fertility rate (P<0.01). In conclusion, although the quality of frozen semen still meets the standards for artificial insemination programs, the age factor in bulls needs to be considered for achieving maximum fertility.

scholarly journals The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia

2021 ◽  
pp. 2473-2481
Author(s):  
Berlin Pandapotan Pardede ◽  
Tulus Maulana ◽  
Ekayanti Mulyawati Kaiin ◽  
Muhammad Agil ◽  
Ni Wayan Kurniani Karja ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Semen Analysis ◽  
Normal Function ◽  
Computer Assisted ◽  
Major Protein ◽  
Protein Marker ◽  
Acridine Orange Staining ◽  
Frozen Semen

Background and Aim: Protamine (PRM) is the major protein in the sperm nucleus and plays an essential role in its normal function. Moreover, PRM has great potential as a protein marker of semen production and quality. This study aimed to assess the potential of sperm bovine PRM as a protein marker of semen production and quality in bulls at the National Artificial Insemination (AI) Center of Indonesia. Materials and Methods: The semen production capacity of each bull was collected from frozen semen production data at the Singosari AI Center for 6 months, and was then divided into two groups (high and low). A total of 440 frozen semen straws from six Limousin (LIM), six Friesian Holstein (FH), six Peranakan Ongole (PO), and four Aceh bulls aged 4-5 years were used in the study. The frozen semen was used to measure the concentration of PRM1, PRM2, and PRM3 using the enzyme immunoassay method. The frozen semen was also used to assess the quality of the semen, including progressive motility (PM) through computer-assisted semen analysis, sperm viability through eosin–nigrosin analysis, and the DNA fragmentation index through Acridine Orange staining. Results: PRM1 was significantly higher in all bull breeds included in the study (p<0.00), followed by PRM2 (p<0.00) and PRM3 (p<0.00). PRM1 significantly affected semen production in LIM, FH, PO, and Aceh bulls (p<0.05). Moreover, PRM2 significantly affected semen production only in FH and Aceh bulls (p<0.05), whereas PRM3 affected this parameter in PO and Aceh bulls exclusively (p<0.05). Consistently and significantly, PRM1 was positively correlated with the PM and viability of sperm and negatively associated with its DNA fragmentation in LIM, FH, PO, and Aceh bulls (p<0.05; p<0.01). The correlation analysis between PRM2 and PRM3 and semen quality parameters varied across all bull breeds; some were positively and negatively correlated (p<0.05; p<0.01), and some were not correlated at all. Conclusion: PRM1 has excellent potential as a protein marker of semen production and quality in bulls at the National AI Center of Indonesia.

scholarly journals Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

2018 ◽  
Vol 43 (1) ◽  
pp. 26 ◽  
Author(s):  
H. C. Mahendra ◽  
D. Samsudewa ◽  
Y. S. Ondho
Keyword(s):  
Artificial Insemination ◽  
Recovery Rate ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Animal Husbandry ◽  
Mean Value ◽  
National Standard ◽  
Frozen Semen ◽  
Standard Values

This study aimed to evaluate and compare the quality of post thawing spermatozoa of buffalo frozen semen produced by artificial insemination centers on standard values, and proposed reference values (PRV). Materials of the research were 60 samples of straws obtained from three Artificial Insemination Center, which are each 20 straws, respectively. Parameters observed were motility, concentration, longevity, plasma membrane integrity (PMI), acrosome integrity (AIn) and recovery rate. The obtained data were tested by Z test then presented as mean ± standard deviation. The research results from three artificial insemination center showed that motility mean was 45.00±3.07%, concentration mean was 26.09±3.11 x106 cells/0.25mL, longevity mean was 10.38±0.75%/hour, PMI mean was 45.86±10.67%, AI mean was 73.99±5.28% and recovery rate mean was 64.38±5.16%. The conclusions of this research were the motility and concentration mean were higher than Indonesian National Standard (INS), longevity mean was lower than Department of Animal Husbandry, Dairying and Fisheries (DADF) standard but PMI and AIn mean were higher, the mean value of recovery rate was higher than of proposal value. 

scholarly journals Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

2021 ◽  
Vol 44 (2) ◽  
pp. 146-151
Author(s):  
Nurcholis Nurcholis ◽  
A. Furqon ◽  
R. I. Arifiantini ◽  
S. M. Salamony
Keyword(s):  
Sperm Motility ◽  
Recovery Rate ◽  
Semen Quality ◽  
Egg Yolk ◽  
Sperm Viability ◽  
Nitrogen Vapor ◽  
Frozen Semen ◽  
Fruit Oil ◽  
Range Test

Antioxidants such as tocopherol, ß-carotene, and polyunsaturated fatty acids (PUFA) from red fruit oil of Papua may be used to protect frozen semen. The study is aimed to test the effect of red fruit oil supplementation on motility, viability, and recovery rate of frozen sperm of Ongole-grade bulls. Semen was collected twice a month from eight 4-5-year-old male Ongole grade using an artificial vagina, followed by macro- and microscopical evaluations. Collected semen was divided into four tubes and diluted with tris egg yolk diluents (TEY) as a control, TEY supplemented with 0.5% red fruit oil (RFO) (TEY RFO0.5), TEY supplemented with 1% RFO (TEY RFO1.0), and TEY supplemented with 1.5% RFO (TEY RFO1.5). The diluted semen was then packed into the straw and equilibrated for 2, 4, and 6 h prior to frozen on liquid nitrogen vapor for 10 minutes. The observed variables in this study were sperm motility, sperm viability, and morphology after equilibration, after thawing, and recovery rate. The experimental design is a completely randomized factorial design. The data were analyzed using ANOVA and were further tested using Duncan multiple range test. The results showed that the sperm motility of fresh semen was 81.10±1.42%. The percentage of sperm motility in TEY RFO1.5 treatment at 6 h equilibration was 60.00±1.06%, significantly higher compared to TEY RFO1.0 and TEY RFO0.5. The percentage of post-thawing sperm motility in TEY RFO1.5 treatment was 62.40±1.09%. The best post-thawing sperm viability in TEY RFO1.5 was 80.70±1.20%, significantly increase from the treatment of TEY RFO1.0 and TEY RFO0.5. The recovery rate (RR) for TEY RFO1.5 treatment had the best percentage at 76.94%. In conclusion, RFO supplementation in semen diluents for 2 h of equilibration resulted in the best motility and viability at 0 h of post thawing observation.

scholarly journals The The Effect of Age and Breed on The Quality of Bull Semen in The Regional Artificial Insemination Centre

2021 ◽  
pp. 132-136
Author(s):  
Agung Budiyanto ◽  
Makruf Arif ◽  
Mandala Phivi Whelma Alfons ◽  
Rifia Tiara Fani ◽  
Ardian Faiz Hafid ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Age Groups ◽  
Bull Semen ◽  
Sperm Abnormalities ◽  
Frozen Semen ◽  
Cattle Reproduction ◽  
Effect Of Age ◽  
Artificial Vagina

The success of beef cattle reproduction development in Indonesia very much depends on the quality of the semen produced by the Indonesian Institute for Artificial Insemination. Good quality bull will benefit farmers, frozen semen producers, and the country in large. This study aimed to determine effect of age and breed on the libido and quality of bull semen collected in the Regional Artificial Insemination Center (RAIC). This study used PO, Simmental, and Brahman Bull in RAIC Yogyakarta, with a total amount of 15, aged 4-7 years old. Semen was collected using an artificial vagina, semen evaluation used a spectrophotometer, and eosin-nigrosin staining method used for sperm viability and abnormalities examination. The result of this study indicated that bull breed did not affect the libido value and the semen quality of bulls (P>0,05). Volume, concentration, motility, and viability in various age groups did not show significant differences (P>0,05), but age groups had a significant effect on sperm abnormalities (P<0,05). The group of bulls aged 5-6 years showed the best quality compared to other groups, with the volume 4,58 ± 1,37 ml, concentration 1,91 ± 0,36 x109/ml, motility 3,80 ± 1,30, viability 89.83 ± 10.69%, and sperm abnormalities 2.51 ± 0.81%. It can be concluded that breed did not affect the bull libido and semen quality, while age had an effect on bull sperm abnormalities.

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

2021 ◽  
Vol 34 (2) ◽  
pp. 198-204 ◽  
Author(s):  
Suherni Susilowati ◽  
Trilas Sardjito ◽  
Imam Mustofa ◽  
Oky Setio Widodo ◽  
Rochmah Kurnijasanti
Keyword(s):  
Pregnancy Rate ◽  
Dna Fragmentation ◽  
Green Tea ◽  
Prostaglandin F2α ◽  
Green Tea Extract ◽  
Control Group ◽  
Bull Semen ◽  
Frozen Semen ◽  
Tea Extract

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

scholarly journals Comparative study of semen quality traits between Etawah grade and Senduro bucks

2021 ◽  
Vol 888 (1) ◽  
pp. 012022
Author(s):  
N Isnaini ◽  
A A Hakim ◽  
D Amertaningtyas ◽  
H E Sulistyo ◽  
A Irsyammawati ◽  
...  
Keyword(s):  
Comparative Study ◽  
Artificial Insemination ◽  
Recovery Rate ◽  
Semen Quality ◽  
T Test ◽  
Quality Traits ◽  
Spermatozoa Motility ◽  
Frozen Semen ◽  
Semen Volume

Abstract This study compared the semen quality traits between Etawah grade (EGB) and Senduro bucks (SB) raised in Singosari National Artificial Insemination Center, Indonesia. A total of 96 ejaculated semen were collected from three EGB and three SB with an average age of three years. The ejaculates were evaluated for semen volume (SV), spermatozoa concentration (SC), total spermatozoa (TS), spermatozoa motility (SM), post-thawing spermatozoa motility (PTSM), the recovery rate of spermatozoa motility (RRSM), and frozen semen production (FSP). Data of SV, SM, PTSM, and RRSM were compared using the Mann-Whitney U test. While the data of SC, TS, and FSP were analyzed using an independent t-test. The results showed that SV, SM, PTSM, and RRSM were not significantly differed (P>0.05) between breeds. Whereas, SC, TS, and FSP of EGB were significantly higher (P<0.05) than SB. Therefore, it can be concluded that Etawah grade bucks can produce higher total spermatozoa and as a result higher frozen semen production for artificial insemination as compared to Senduro bucks.

scholarly journals COMBINED EFFECT OF STREPTOMYCIN AND SWEET ORANGE ESSENTIAL OIL TO MEMBRANE AND ACROSOME INTEGRITY BOER GOAT FROZEN SEMEN

2019 ◽  
Vol 3 (2) ◽  
pp. 89
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Boer Goat ◽  
Randomized Design ◽  
Frozen Semen ◽  
Acrosome Integrity ◽  
Completely Randomized Design ◽  
Orange Essential Oil

One of factors that cause a bad quality of Boer Goat frozen semen is the growth of bacterial. This can be overcome by adding antibiotics such as streptomycin. To further suppress the growth of bacteria can be added other ingredients that contain antibacterials such as sweet orange essential oil. The purpose of this research is to know the percentage value of Membrane Integrity and Acrosome Integrity on Boer Goat frozen semen with addition sweet orange essential oil and streptomycin. The method used was experimental using Completely Randomized Design with 5 treatments and 5 replications. The treatment in this research is addition 0%, 0,25%; 0.5%; 0.75% and 1% sweet orange essential oil on tris yolk and streptomycin extender. The results showed the best treatment addition combination streptomycin and sweet orange essential oil to percentage Membrane Integrity and Acrosome Integrity is increase 1% sweet orange essential oil.Keywords: Boer Goat, essential oil, frozen semen, streptomycin, sweet orange.

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