scholarly journals Effect of hypoxia tolerance on the relation between indicators of free radical oxidation of lipides and proteins in murine kidneys during the post-resuscitation period

2017 ◽  
Vol 98 (6) ◽  
pp. 949-954 ◽  
Author(s):  
G A Bayburina ◽  
E A Nurgaleeva ◽  
E F Agletdinov ◽  
A F Samigullina
Keyword(s):  
Free Radical ◽  
Free Radical Oxidation ◽  
Systemic Circulation ◽  
Oxidative Modification ◽  
Hypoxia Tolerance ◽  
Oxidative Modification Of Proteins ◽  
Radical Oxidation ◽  
Resistance To Hypoxia ◽  
The Relationship ◽  
Observation Period

Aim. Study of the relationship between the parameters of free radical oxidation of proteins and lipids in the murine kidneys in the post-resuscitation period after stopping the systemic circulation, depending on their resistance to hypoxia. Methods. The systemic circulation was stopped by intra-thoracic clamping of the neurovascular bundle for 5 minutes, performed under general ether anesthesia in male noninbred white rats, divided after testing into two groups based on resistance to hypoxia. The observation period lasted for 35 days. In the homogenates of kidney tissues, the content of products reactive to tiobarbituric acid, carbonylated proteins, the formation of metal-catalyzed carbonylated proteins and bitirozin were determined. Results. The characteristic manifestation of oxidative stress in the recovery period after stopping blood circulation and resuscitation was found to be reciprocity of the relationship between the levels of lipoperoxidation and oxidative modification of proteins. Highly resistant to hypoxia animals were characterized by high resistance of proteins of kidney tissue to free radical oxidation against the background of high levels of lipid peroxidation. On the contrary, in animals non-resistant to hypoxia, against the background of relatively low values of lipoperoxidation, high levels of oxidative modification of proteins, both initial and induced, were recorded. Conclusion. In post-resuscitation period in highly resistant to hypoxia animals, marked activation of lipoperoxidation occurs accompanied by a transient increase in the carbonylation of proteins in the early observation period; for low-resistant to hypoxia animals high intensity of carbonyl stress against the background of the relative «preservation» of lipid structures of the cell is characteristic, which persists throughout the post-resuscitation period, which can make a significant contribution to kidney damage, increasing the risk of renal failure.

scholarly journals Association of blood and liver free radical oxidation indicators in rats with different resistance to hypoxia after survived anoxia

2015 ◽  
Vol 96 (5) ◽  
pp. 798-802
Author(s):  
G A Bayburina ◽  
E A Nurgaleeva ◽  
S A Bashkatov ◽  
D Z Shibkova
Keyword(s):  
Antioxidant Activity ◽  
Factor Analysis ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Thiobarbituric Acid ◽  
Systemic Circulation ◽  
Antioxidant Systems ◽  
Radical Oxidation ◽  
Resistance To Hypoxia ◽  
White Rats

Aim. To study the association of rat liver and blood free radical oxidation indicators with different resistance to hypoxia on the long run after ischemic damage caused by systemic circulation arrest. Methods. Circulatory arrest lasting for 5 minutes was modeled at ether anesthesia on male white rats which were further allocated to 3 groups according to resistance to hypoxia. The observation period was 35 days. The levels of reduced glutathione, catalase, and products reacting with thiobarbituric acid were determined in liver homogenates, levels of erythrocyte catalase, total antioxidant activity, glutathione peroxidase, glutathione transferase, and products reacting with thiobarbituric acid - in blood. Statistical analysis was performed by parametric statistics, regression and factor analysis. Results. Rats with different resistance to hypoxia initially differed significantly on the number of safety systems functioning indicators levels in liver tissue and blood cells. Total factor analysis of the entire body of empirical data revealed two factors, conventionally called «resistance to hypoxia» and «antioxidant activity of blood». Daily analysis of the figures recorded at 1, 3, 5, 7, 14, 21 and 35 day of the experiment revealed similarities in common and daily factor solutions, demonstrating the objectivity of the revealed patterns, which emphasizes the significant role of free radical oxidation in anoxic conditions pathogenesis. Conclusion. The balance pro- and antioxidant systems activity in the liver and blood is an essential component of the body’s resistance to hypoxia affecting the survival of the animals after systemic circulation arrest. Their level predicts resistance to hypoxia at the recovery period.

Clinical and pathogenetic role of state of prooxidant-antioxidant regulation in patients with purulent meningitis in the dynamics of disease

2014 ◽  
Vol 18 (4 (72)) ◽  
Author(s):  
D. A. Zadyraka ◽  
E. V. Riabokon
Keyword(s):  
Free Radical ◽  
Conventional Treatment ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Blood Proteins ◽  
Radical Oxidation ◽  
Pathogenetic Role ◽  
Purulent Meningitis ◽  
Laboratory Changes

The paper shows, that patients with purulent meningitis on the background of severe clinical and laboratory changes had a disturbance of prooxidant-antioxidant regulation with a shift towards activation of free radical oxidation. In the dynamics of the disease, after 7-10 days of conventional treatment, against the background of a reduction of cephalic and meningeal syndromes intensity, a normalization of liquor and hemogram indices, in most patients there was a further decrease in catalase activity and an increase of nitrites, of parameters of spontaneous and induced oxidative modification of blood proteins that remained when the patients were discharged, compared with healthy people.

scholarly journals Genetic peculiarities of free radical oxidation of lipids and proteins in the semen of breeding boars

Biologija ◽  
2018 ◽  
Vol 64 (3) ◽  
Author(s):  
Svitlana Polishchuk ◽  
Svitlana Tsekhmistrenko ◽  
Vitalii Polishchuk ◽  
Oksana Tsekhmistrenko ◽  
Nataliia Ponomarenko ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Reproductive Function ◽  
Oxidative Modification ◽  
Peroxide Oxidation ◽  
Large White ◽  
Radical Oxidation ◽  
Key Enzyme ◽  
Synthetic Line

Complex research into biochemical aspects of the functioning of the antioxidant system for the protection of sperm of the breeding boars has established that the intensity of the flow of peroxide oxidation of lipids in germ cells is much higher than in the extracellular space. Reactions of free radical peroxide oxidation of proteins and lipids in the semen of healthy breeding boars are characterized by a stable level of activity that is necessary for the normal course of implementation of processes of the reproductive function. All components of the antioxidant system stay in mutual compensatory ratios under physiological conditions. As a rule, reducing the concentration or activity of some antioxidant enzymes leads to corresponding changes in others. The main antioxidants of the germ cell genomes are superoxide dismutase and ceruloplasmin. Catalase is a key enzyme that neutralizes H2O2 in the semen fluid of breeding boars. The content of total proteins in the semen of the synthetic line SS23 animals is greater (p < 0.05) than in the breeding boars of the large white breed. The processes of the oxidative modification of proteins in the animals body of the synthetic line runs more intensely, as it is evidenced by the higher content of the aldehyde and ketodinitrophenyl hydrazones of the main and neutral character in the sperm cytoplasm.

The relationship between neuroendocrine dysfunction and free-radical oxidation in old age alcoholism

2015 ◽  
Vol 115 (8) ◽  
pp. 73
Author(s):  
D. B. Vinogradov ◽  
A. Kh. Mingazov ◽  
I. V. Izarovskaya ◽  
K. A. Babin ◽  
A. I. Sinitsky
Keyword(s):  
Free Radical ◽  
Old Age ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
The Relationship

scholarly journals Effect of free radical oxidation on content of corticosteroid receptors in the lungs of animals with different sensitivity to hypoxia in the postresuscitation period

2019 ◽  
pp. 56-63
Author(s):  
Г.А. Байбурина ◽  
Е.А. Нургалеева ◽  
А.Ф. Самигуллина ◽  
Э.Ф. Аглетдинов
Keyword(s):  
Free Radical ◽  
Glucocorticoid Receptors ◽  
Recovery Period ◽  
Free Radical Oxidation ◽  
Mineralocorticoid Receptors ◽  
Ether Anesthesia ◽  
Radical Oxidation ◽  
Resistance To Hypoxia ◽  
Corticosteroid Receptors ◽  
Late Recovery

Цель исследования - изучение особенностей динамики показателей свободнорадикального окисления и уровня кортикостероидных рецепторов в легких у животных с разной чувствительностью к гипоксии в постреанимационном периоде. Методика. Эксперимент выполнен на самцах беспородных белых крыс, разделенных по устойчивости к гипоксии на 2 группы: неустойчивые и высокоустойчивые. Остановку системного кровообращения продолжительностью 5 мин моделировали под общим эфирным наркозом (через 1 нед после тестирования на устойчивость к гипоксии) интраторакальным пережатием сосудистого пучка сердца с последующей реанимацией. Через 1, 3, 5, 7, 14, 21 и 35 сут животных под эфирным наркозом декапитировали. В плазме крови определяли содержание кортикостерона и альдостерона, в гомогенатах легких - концентрацию глюкокортикоидных и минералокортикоидных рецепторов, уровни продуктов, реагирующих с тиобарбитуровой кислотой, карбонилированных белков, железозависимое образование битирозина, активность супероксиддисмутазы, каталазы, содержание восстановленного глутатиона. Результаты. Установлено, что для неустойчивых к гипоксии животных характерна высокая интенсивность свободнорадикального окисления, проявляющаяся развитием карбонильного стресса на фоне снижения активности ключевых антиоксидантов и сопряженная с выраженными изменениями динамики кортикостероидных рецепторов: в первые 3 сут постреанимационного периода наблюдалось снижение уровня кортикостероидных рецепторов, а к концу 35-суточного мониторинга значительное нарастание концентрации минералокортикоидных рецепторов. Высокую степень устойчивости к гипоксии отличает относительно низкая интенсивность свободнорадикального окисления, характеризующаяся только усилением липопероксидации за счет адекватной емкости и сохранности водорастворимых антиоксидантных систем, экранирующих белки. В раннем восстановительном периоде это сопровождается сохранением уровня кортикостероидных рецепторов, в позднем - существенным ростом концентрации глюкокортикоидных рецепторов. Заключение. Увеличение содержания глюкокортикоидных рецепторов у высокоустойчивых к гипоксии животных в позднем восстановительном периоде (14-е - 35-е сут) играет важную роль, предположительно путем модулирования течения воспалительного процесса, ограничения неконтролируемых иммунных реакции и повреждение тканей. Следствием значительного нарастания концентрации минералокортикоидных рецепторов у животных с низкой устойчивостью к гипоксии может быть усиление сосудистого ремоделирования и развитие фиброза в легких. Aim. The aim of the study was to characterize changes in free radical oxidation and corticosteroid receptor density in the lungs of animals with different sensitivity to hypoxia in the postresuscitation period. Methods. Experiments were performed on mongrel male white rats divided into two groups based on their hypoxia resistance, non-resistant and highly resistant. One week after testing the rats for resistance to hypoxia, a 5-min arrest of systemic circulation was modeled under ether anesthesia by intrathoracic compression of the vascular bundle of the heart with subsequent resuscitation. The follow-up period was 35 days. At 1, 3, 5, 7, 14, 21, and 35 days, the animals were sacrificed by decapitation under ether anesthesia, and blood and tissue samples were collected. Concentrations of corticosterone and aldosterone were measured in plasma; concentrations of glucocorticoid and mineralocorticoid receptors, thiobarbituric acid reactive substances, and carbonylated proteins, iron-dependent formation of dityrosine, activities of superoxide dismutase and catalase, and concentration of reduced glutathione were measured in lung homogenates. Results. Hypoxia non-resistant rats had a high intensity of free radical oxidation as evident from the development of carbonyl stress associated with decreased activities of key antioxidants and pronounced changes in the dynamics of corticosteroid receptors. A reduced level of corticosteroid receptors was observed in the first three days of resuscitation period and followed by a significant increase in mineralocorticoid receptors at the end of 35-day monitoring. High resistance to hypoxia was characterized by a relatively low intensity of free radical oxidation, evident only from increased lipid peroxidation, due to an adequate capacity and preservation of water-soluble antioxidants. During the early recovery period, high resistance to hypoxia was associated with preserved level of corticosteroid receptors whereas during late recovery, the high hypoxia resistance was associated with a significant increase in the concentration of glucocorticoid receptors. Conclusion. The increased density of glucocorticoid receptors in hypoxia high-resistant animals during the late recovery period (days 14-35) plays an important role, presumably by modulating the inflammatory process and restricting uncontrolled immune responses and tissue damage. The significant increase in mineralocorticoid receptors in hypoxia low-resistance animals may result in stimulation of vascular remodeling and development of pulmonary fibrosis.

scholarly journals Chronophered approach to appointment of patients with alergodromatosis of antioxidant drugs

2019 ◽  
pp. 11-16
Author(s):  
Ольга Денисенко
Keyword(s):  
Atopic Dermatitis ◽  
Reduced Glutathione ◽  
Clinical Manifestations ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Complex Treatment ◽  
Daily Rhythms ◽  
Oxidative Modification Of Proteins ◽  
Radical Oxidation ◽  
Oxidation Processes

Objective - to increase the efficiency of treatment of pa­tients with allergic dermatitis by using chronodermined antioxidant drugs, taking into account the daily rhythms of the parameters of prooxidant antioxidant homeostasis. Materials and methods: 73 patients with allergic derma­titis (eczema, allergic and atopic dermatitis) were exam­ined, which determined the circadian (daily) rhythms of the parameters of prooxidant-antioxidant homeostasis. Results and discussion. In patients with allergic der­matitis, an increase in the mesor and amplitude of ma­lonic aldehyde, the fractions of oxidative modification of proteins and mediomolecular peptides with acrophase in the evening hours, as well as the reduction of the mesor of reduced glutathione and ceruloplasmin with batifase in evening hours, has been established, indicating activa­tion and growth during free radical oxidation processes lipids and proteins against the background of a decrease in the activity of antioxidant flora, which substantiates the chronoderminable purpose of such a patient with antioxidant drugs. Conclusion: Chronodetermined ap­pointment in the complex treatment of allergic dermati­tis antioxidant drugs accelerates the regression of their clinical manifestations, reduces the timing of treatment of patients, as well as helps to normalize their prooxidant antioxidant homeostasis.

scholarly journals Aspects of free radical oxidation in the large bowel in ulcerative colitis and Crohn’s disease

2020 ◽  
pp. 58-64
Author(s):  
MV Osikov ◽  
EV Davydova ◽  
MS Boyko ◽  
AE Bakeeva ◽  
NV Kaygorodtseva ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Free Radical ◽  
Crohn's Disease ◽  
Large Bowel ◽  
Activity Index ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Trinitrobenzenesulfonic Acid ◽  
Radical Oxidation

Research into the accumulation patterns of protein oxidative modification (POM) products and lipids in Crohn’s disease (CD) and ulcerative colitis (UC) could have important implications for understanding the pathogenesis and improving the diagnosis and therapy for these diseases. The aim of this study was to investigate the aspects of free radical oxidation (FRO) in the large bowel and their possible correlations with clinical symptoms of UC and CD. In the Wistar rat model used in the experiment, CD was induced with 2,4,6-trinitrobenzenesulfonic acid, and UC was induced with oxazolone. Clinical status was assessed using the Disease activity index (DAI). Lipid peroxidation (LPO) products were measured in the heptane and isopropanol phases of the intestinal mucosa extract. POM products were measured following spontaneous and stimulated oxidation. The DAI ( Me (Q25–Q75)) was increased in both CD and UC on days 3 and 7 of the experiment: for CD, it was equally increased on days 3 and 7 (7 (3-7)) and was 11 (11–11) and 11 (9–11) for UC on days 3 and 7, respectively. The amount of primary, secondary and end LPO products in the heptane and isopropanol phases, as well as the total amount of POM products, was increased in the homogenized mucosa of the large bowel. In the CD group, the relative content of secondary basic POM products was increased on day 7 of the experiment. The following patterns of FRO were revealed: accumulation of LPO products in the UC group and accumulation of POM products in the CD group; UC is characterized by the accumulation of mostly LPO products in the heptane phase and secondary LPO products in the isopropanol phase; CD is characterized by the accumulation of secondary basic POM products. DAI scores were correlated with the amount of LPO products in the isopropanol phase and the amount of POM products in the spontaneous oxidation mode. The highest number of strong correlations was observed in the UC group. Our findings suggest a very serious contribution of FRO changes to the pathogenesis of UC and CD, meaning that LPO and POM products could be regarded as diagnostic markers and indicators of treatment efficacy.

scholarly journals Limited proteolysis and oxidative modification of proteins in the hepatocytes of patients with resistant forms of tuberculosis

Pneumologia ◽  
2019 ◽  
Vol 68 (3) ◽  
pp. 126-129
Author(s):  
Lilia Todoriko ◽  
Ihor Semianiv ◽  
Radu Criṣan-Dabija ◽  
Olʼha S. Shevchenko
Keyword(s):  
Free Radical ◽  
Protein Modification ◽  
Comparison Group ◽  
Limited Proteolysis ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Bromphenol Blue ◽  
Oxidative Modification Of Proteins ◽  
Pulmonary Tb ◽  
Oxidative Protein Modification

Abstract The aim of this paper was to evaluate the oxidative modification of proteins and limited proteolysis in the hepatocytes of patients with resistant forms of pulmonary tuberculosis (TB). Material and method: This is a prospective pathomorphological study of 60 patients with confirmed pulmonary TB (clinically and anatomopathologically) deaths from various causes. To evaluate the intensity of free radical processes in hepatocytes, we used a methodology developed by Davydenko IS, 2003. Histological sections were stained with bromphenol blue by Michel-Calvo. Results: When comparing the digital data indicated in our study, it is evident that changes in proteins properties in average tendencies run virtually identically in the hepatocytes of different zones of the comparison group. Thus, the difference is unlikely (p > 0.05) when comparing red/blue ratio (specific staining of acid and basic proteins by Michel-Calvo) in the hepatocytes of zones I, II and III of the comparison group. A similar trend is observed in the analysis of average values of the optical density of a specific colouration on the free amino group of proteins by A. Yasuma and T. Ichikava. The analysis of all parameters showed a linear increase in the intensity of oxidative protein modification and limited proteolysis from zone I to III (from the periportal to the centrolobular parts of the acinus) in all subgroups of the main group according to the acinus zone (by Rappoport). Conclusions: Pulmonary TB leads to increased processes of free radical oxidation of proteins with characteristic effects – increased proteolysis and oxidation of amino groups of proteins – in the hepatocytes of the liver.

scholarly journals Окиснювальна модифікація білків у щурів різного віку за умов гострого отруєння токсинами блідої поганки

2017 ◽  
Author(s):  
I. P. Kuzmak ◽  
Ye. B. Dmukhalska ◽  
S. R. Pydruchna ◽  
T. Ya Yaroshenko ◽  
I. Ya. Krynytska ◽  
...  
Keyword(s):  
Age Groups ◽  
Intraperitoneal Administration ◽  
Free Radical Oxidation ◽  
Numerical Data ◽  
Pathological Process ◽  
Oxidative Modification ◽  
Amanita Phalloides ◽  
Oxidative Modification Of Proteins ◽  
Radical Oxidation ◽  
Protein Oxidative Modification

Introduction. In recent years, scientific articles of domestic and foreign scientists obtained numerical data on the important role of oxidative modification of proteins in the development of many diseases. It is known that entering the organism of toxic compounds, including toxins of Amanita phalloides, sharply oppress antioxidant system increases the contents of peroxidation products lipids and proteins in liver, kidneys.The aim of the study – to examine the dynamics of oxidative modification of proteins in rats of different ages affected by poison Amanita phalloides toxins in different periods of the experiment.Methods of the research. In all experimental groups poisoning was modeled by single intraperitoneal administration Amanita phalloides extract obtained by the method of H. Wieland (85 mg / kg body weight). Euthanasia was performed under sodium thiopental anesthesia at 6, 24 and 72 hours after poisoning. Oxidative modification of proteins in blood serum was investigated by Meschyshen method.Results and Discussion. Protein oxidative modification indices in all age groups rats on the model of toxic injury by Amanita phalloides toxins were investigated. It was found out that the progression of the pathological process in the liver and the depletion of the compensatory capacity in animals with acute poisoning by Amanita phalloides leads to the activation of free radical oxidation of proteins and cause the increasing of protein oxidative modification indices. The most pronounced changes occur in 6- and 18- month rats in 24 hours after intoxication.Conclusions. It was defined, that the progression of the pathological process in the liver and the depletion of the compensatory capacity as a result of poisoning by toxins of Amanita phalloides cause the increasing of protein oxidative modification indices. It can be considered as one of the earliest markers of damage to organs and tissues including hepatocytes and reliable early predictor of the development and pathology related oxidative stress.

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