scholarly journals Окиснювальна модифікація білків у щурів різного віку за умов гострого отруєння токсинами блідої поганки

2017 ◽  
Author(s):  
I. P. Kuzmak ◽  
Ye. B. Dmukhalska ◽  
S. R. Pydruchna ◽  
T. Ya Yaroshenko ◽  
I. Ya. Krynytska ◽  
...  
Keyword(s):  
Age Groups ◽  
Intraperitoneal Administration ◽  
Free Radical Oxidation ◽  
Numerical Data ◽  
Pathological Process ◽  
Oxidative Modification ◽  
Amanita Phalloides ◽  
Oxidative Modification Of Proteins ◽  
Radical Oxidation ◽  
Protein Oxidative Modification

Introduction. In recent years, scientific articles of domestic and foreign scientists obtained numerical data on the important role of oxidative modification of proteins in the development of many diseases. It is known that entering the organism of toxic compounds, including toxins of Amanita phalloides, sharply oppress antioxidant system increases the contents of peroxidation products lipids and proteins in liver, kidneys.The aim of the study – to examine the dynamics of oxidative modification of proteins in rats of different ages affected by poison Amanita phalloides toxins in different periods of the experiment.Methods of the research. In all experimental groups poisoning was modeled by single intraperitoneal administration Amanita phalloides extract obtained by the method of H. Wieland (85 mg / kg body weight). Euthanasia was performed under sodium thiopental anesthesia at 6, 24 and 72 hours after poisoning. Oxidative modification of proteins in blood serum was investigated by Meschyshen method.Results and Discussion. Protein oxidative modification indices in all age groups rats on the model of toxic injury by Amanita phalloides toxins were investigated. It was found out that the progression of the pathological process in the liver and the depletion of the compensatory capacity in animals with acute poisoning by Amanita phalloides leads to the activation of free radical oxidation of proteins and cause the increasing of protein oxidative modification indices. The most pronounced changes occur in 6- and 18- month rats in 24 hours after intoxication.Conclusions. It was defined, that the progression of the pathological process in the liver and the depletion of the compensatory capacity as a result of poisoning by toxins of Amanita phalloides cause the increasing of protein oxidative modification indices. It can be considered as one of the earliest markers of damage to organs and tissues including hepatocytes and reliable early predictor of the development and pathology related oxidative stress.

scholarly journals Chronophered approach to appointment of patients with alergodromatosis of antioxidant drugs

2019 ◽  
pp. 11-16
Author(s):  
Ольга Денисенко
Keyword(s):  
Atopic Dermatitis ◽  
Reduced Glutathione ◽  
Clinical Manifestations ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Complex Treatment ◽  
Daily Rhythms ◽  
Oxidative Modification Of Proteins ◽  
Radical Oxidation ◽  
Oxidation Processes

Objective - to increase the efficiency of treatment of pa­tients with allergic dermatitis by using chronodermined antioxidant drugs, taking into account the daily rhythms of the parameters of prooxidant antioxidant homeostasis. Materials and methods: 73 patients with allergic derma­titis (eczema, allergic and atopic dermatitis) were exam­ined, which determined the circadian (daily) rhythms of the parameters of prooxidant-antioxidant homeostasis. Results and discussion. In patients with allergic der­matitis, an increase in the mesor and amplitude of ma­lonic aldehyde, the fractions of oxidative modification of proteins and mediomolecular peptides with acrophase in the evening hours, as well as the reduction of the mesor of reduced glutathione and ceruloplasmin with batifase in evening hours, has been established, indicating activa­tion and growth during free radical oxidation processes lipids and proteins against the background of a decrease in the activity of antioxidant flora, which substantiates the chronoderminable purpose of such a patient with antioxidant drugs. Conclusion: Chronodetermined ap­pointment in the complex treatment of allergic dermati­tis antioxidant drugs accelerates the regression of their clinical manifestations, reduces the timing of treatment of patients, as well as helps to normalize their prooxidant antioxidant homeostasis.

scholarly journals Effect of hypoxia tolerance on the relation between indicators of free radical oxidation of lipides and proteins in murine kidneys during the post-resuscitation period

2017 ◽  
Vol 98 (6) ◽  
pp. 949-954 ◽  
Author(s):  
G A Bayburina ◽  
E A Nurgaleeva ◽  
E F Agletdinov ◽  
A F Samigullina
Keyword(s):  
Free Radical ◽  
Free Radical Oxidation ◽  
Systemic Circulation ◽  
Oxidative Modification ◽  
Hypoxia Tolerance ◽  
Oxidative Modification Of Proteins ◽  
Radical Oxidation ◽  
Resistance To Hypoxia ◽  
The Relationship ◽  
Observation Period

Aim. Study of the relationship between the parameters of free radical oxidation of proteins and lipids in the murine kidneys in the post-resuscitation period after stopping the systemic circulation, depending on their resistance to hypoxia. Methods. The systemic circulation was stopped by intra-thoracic clamping of the neurovascular bundle for 5 minutes, performed under general ether anesthesia in male noninbred white rats, divided after testing into two groups based on resistance to hypoxia. The observation period lasted for 35 days. In the homogenates of kidney tissues, the content of products reactive to tiobarbituric acid, carbonylated proteins, the formation of metal-catalyzed carbonylated proteins and bitirozin were determined. Results. The characteristic manifestation of oxidative stress in the recovery period after stopping blood circulation and resuscitation was found to be reciprocity of the relationship between the levels of lipoperoxidation and oxidative modification of proteins. Highly resistant to hypoxia animals were characterized by high resistance of proteins of kidney tissue to free radical oxidation against the background of high levels of lipid peroxidation. On the contrary, in animals non-resistant to hypoxia, against the background of relatively low values of lipoperoxidation, high levels of oxidative modification of proteins, both initial and induced, were recorded. Conclusion. In post-resuscitation period in highly resistant to hypoxia animals, marked activation of lipoperoxidation occurs accompanied by a transient increase in the carbonylation of proteins in the early observation period; for low-resistant to hypoxia animals high intensity of carbonyl stress against the background of the relative «preservation» of lipid structures of the cell is characteristic, which persists throughout the post-resuscitation period, which can make a significant contribution to kidney damage, increasing the risk of renal failure.

Clinical and pathogenetic role of state of prooxidant-antioxidant regulation in patients with purulent meningitis in the dynamics of disease

2014 ◽  
Vol 18 (4 (72)) ◽  
Author(s):  
D. A. Zadyraka ◽  
E. V. Riabokon
Keyword(s):  
Free Radical ◽  
Conventional Treatment ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Blood Proteins ◽  
Radical Oxidation ◽  
Pathogenetic Role ◽  
Purulent Meningitis ◽  
Laboratory Changes

The paper shows, that patients with purulent meningitis on the background of severe clinical and laboratory changes had a disturbance of prooxidant-antioxidant regulation with a shift towards activation of free radical oxidation. In the dynamics of the disease, after 7-10 days of conventional treatment, against the background of a reduction of cephalic and meningeal syndromes intensity, a normalization of liquor and hemogram indices, in most patients there was a further decrease in catalase activity and an increase of nitrites, of parameters of spontaneous and induced oxidative modification of blood proteins that remained when the patients were discharged, compared with healthy people.

scholarly journals Intensity of cardiac free-radicals processes and expression of glutathione peroxidase and glutathione reductase genes in rats with adrenaline

2012 ◽  
Vol 58 (5) ◽  
pp. 530-538 ◽  
Author(s):  
I.Y. Iskusnykh ◽  
T.N. Popova ◽  
O.S. Musharova
Keyword(s):  
Free Radical ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Free Radical Oxidation ◽  
Pathological Process ◽  
Mrna Levels ◽  
Maximal Increase ◽  
Radical Oxidation ◽  
Polymerase Chain ◽  
Radical Processes

The correlation between changes in activities of glutathione peroxidase and glutathione reductase in heart of rats during development of adrenaline myocarditis and intensity of free radical processes estimated by biochemiluminesce parameters and the content of lipoperoxidation products was demonstrated. The maximal increase of glutathione peroxidase and glutathione reductase activities (in 1.8 and 1.4 times accordingly) was observed t 24 h after the development of the pathological process; this coincided with the maximum intensity of prosesses of free radical oxidation. Using combination of reverse transcriptions with real-time polymerase chain reaction the cardiac mRNA levels of glutathione peroxidase and glutathione reductase genes were determined during the development of adrenaline myocarditis in rats. Analysis of expression of glutathione peroxidase and glutathione reductase genes showed, that the level of this transcripts demonstrated 2,8- and 7,3- increase in rats with adrenaline myocarditis, respectively. Obviously, overexpression of these enzymes can increase the resistance of cardiomyocites to oxidative stress.

scholarly journals Genetic peculiarities of free radical oxidation of lipids and proteins in the semen of breeding boars

Biologija ◽  
2018 ◽  
Vol 64 (3) ◽  
Author(s):  
Svitlana Polishchuk ◽  
Svitlana Tsekhmistrenko ◽  
Vitalii Polishchuk ◽  
Oksana Tsekhmistrenko ◽  
Nataliia Ponomarenko ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Reproductive Function ◽  
Oxidative Modification ◽  
Peroxide Oxidation ◽  
Large White ◽  
Radical Oxidation ◽  
Key Enzyme ◽  
Synthetic Line

Complex research into biochemical aspects of the functioning of the antioxidant system for the protection of sperm of the breeding boars has established that the intensity of the flow of peroxide oxidation of lipids in germ cells is much higher than in the extracellular space. Reactions of free radical peroxide oxidation of proteins and lipids in the semen of healthy breeding boars are characterized by a stable level of activity that is necessary for the normal course of implementation of processes of the reproductive function. All components of the antioxidant system stay in mutual compensatory ratios under physiological conditions. As a rule, reducing the concentration or activity of some antioxidant enzymes leads to corresponding changes in others. The main antioxidants of the germ cell genomes are superoxide dismutase and ceruloplasmin. Catalase is a key enzyme that neutralizes H2O2 in the semen fluid of breeding boars. The content of total proteins in the semen of the synthetic line SS23 animals is greater (p < 0.05) than in the breeding boars of the large white breed. The processes of the oxidative modification of proteins in the animals body of the synthetic line runs more intensely, as it is evidenced by the higher content of the aldehyde and ketodinitrophenyl hydrazones of the main and neutral character in the sperm cytoplasm.

scholarly journals Influence of the preparation “Bendamin” on the indicators of antioxidant protection of rat myocardium in experimental modeling of heart failure

2019 ◽  
Vol 21 (95) ◽  
pp. 98-101
Author(s):  
I. S. Varkholiak ◽  
B. V. Gutyj
Keyword(s):  
Intraperitoneal Administration ◽  
Clinical Signs ◽  
Free Radical Oxidation ◽  
Feed Additives ◽  
Control Group ◽  
Standard Diet ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Experimental Group

The aim of this study was to investigate the effect of Bendamin on the antioxidant status of rats in experimental doxorubicin-induced cardiomyopathy. The studies were performed on white sexually mature young male of Vistar line rats weighing 180–200 g, which were kept on a standard diet of the Institute Vivarium of the State Research Institute of Veterinary Drugs and Feed Additives. Animals were divided into 3 groups of 6 animals in each: control group – intact animals; experimental group R1, in which animals were simulated with doxorubicin-induced cardiomyopathy by intraperitoneal administration of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks; experimental group R2, in which animals were injected with the drug “Bendamin” at a dose of 20 mg/kg after injection of doxorubicin. The data obtained indicate that the introduction into the experimental animals of the experimental group of doxorubicin is accompanied by intensification in the myocardium of the processes of free radical oxidation. The increase in diene conjugates was found to be 32.7% and TBK-active products increased by 37.6% compared to the control group of rats. Doxorubicin was also found to cause inhibition of the glutathione system of the animal's antioxidant protection after administration to rats. After administration of the drug “Bendamin”, rats of the second experimental group were suppressed with lipid peroxidation under the conditions of doxorubicin intoxication. In the rats of the second experimental group, the level of intermediates and end products is likely to decrease, so in the rat myocardial homogenate the level of diene conjugates decreased by 16.8% and the level of TBA-active products by 20.8% compared to the first experimental group. who had clinical signs of cardiomyopathy caused by doxorubicin administration. The use of the drug “Bendamin” in rats of the second experimental group contributed to the enhancement of the enzymatic and non-enzymatic element of the antioxidant system, protecting the structural and functional integrity of cell bioembranes. The results of the studies enrich the pharmacological characteristics of the drug “Bendamin”, indicate its sufficiently clear protective effect on the myocardium in experimental doxorubicin cardiomyopathy and is a convincing proof of the feasibility of the drug in veterinary practice.

scholarly journals Correction of free radical processes and Mitochondrial dysfunction in rats poisoned by sodium nitrite and tobacco smoke, mildronate preparation

2017 ◽  
Author(s):  
P. H. Lykhatskyi
Keyword(s):  
Free Radical ◽  
Sodium Nitrite ◽  
Tobacco Smoke ◽  
Age Groups ◽  
Free Radical Oxidation ◽  
Premature Death ◽  
The Body ◽  
Mitochondrial Enzymes ◽  
Radical Oxidation ◽  
Different Ages

Introduction. According to the WHO, smoking takes the second place in the list of reasons that cause premature death of people. The interest in clarifying the mechanisms of action on the organism of nitrites and nitrates and the links of the pathogenesis of gemic hypoxia, which thus arises, is due to their widespread use in industry, agriculture and medicine. Therefore, the study of the combined effects of several xenobiotics on the body is feasible and relevant.The aimof the study –to investigate the effectiveness of using antihydroxant Mildronate and its influence on the oxidative processes in the body of rats of different ages infected with sodium nitrite on the background of 45 days of tobacco intoxication.Research methods. Under the conditions of damage to rats of different ages, sodium nitrite on the background of tobacco intoxication was determined in the blood of the content of methemoglobin (MetHb) in reaction with acetone cyanadirin and carboxyhemoglobin (HbCO) in reaction with hexacyanone (III) potassium. The activity of lipoperoxidation processes was assessed by the content of TBА – active products (TBА – AP) in the serum of blood, lungs and myocardium of experimental animals. The evaluation of the functioning of the bioenergetic processes was carried out using the activity of succinate dehydrogenase (SAD), which was studied by the reaction of reduction of potassium ferricyanide, which solution has a yellow color, to colorless ferrocyanide of potassium succinate by the action of EDA and cytochrome oxidase (CO) by the oxidation reaction of dimethyl-n-phenylenediamine.Results and Discussion. It has been established that the defeat of rats of different age groups by sodium nitrite (24 and 72 h before the end of the experiment) on the background of 45 days intoxication with tobacco smoke leads to the activation of free radical oxidation processes, indicating an increase in the content of methemoglobin in the blood of affected animals and TBА-AP in serum of blood, lungs and myocardium after poisoning. At the same time in the blood the content of carboxyhemoglobin increases. In the body, there is a mixed hypoxia (hemitic and circulatory), which leads to disturbances in the functioning of the mitochondrial oxidation chain. The most susceptible to the activity of toxicotoxins were immature animals, and in older animals there was a significant change in the activity of mitochondrial enzymes in the myocardium after the injury, which was more pronounced in comparison with other age groups of rats. In order to correct the revealed violations, a drug of metabolic action with antihypoxant properties of mildronate was used, which positively influenced the activity of lipoperoxidation processes and the activity of mitochondrial enzymes under hypoxia conditions.Conclusions. The obtained results indicate the expediency of inclusion of mildronate in the complex treatment of poisonings of different genesis.

scholarly journals Aspects of free radical oxidation in the large bowel in ulcerative colitis and Crohn’s disease

2020 ◽  
pp. 58-64
Author(s):  
MV Osikov ◽  
EV Davydova ◽  
MS Boyko ◽  
AE Bakeeva ◽  
NV Kaygorodtseva ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Free Radical ◽  
Crohn's Disease ◽  
Large Bowel ◽  
Activity Index ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Trinitrobenzenesulfonic Acid ◽  
Radical Oxidation

Research into the accumulation patterns of protein oxidative modification (POM) products and lipids in Crohn’s disease (CD) and ulcerative colitis (UC) could have important implications for understanding the pathogenesis and improving the diagnosis and therapy for these diseases. The aim of this study was to investigate the aspects of free radical oxidation (FRO) in the large bowel and their possible correlations with clinical symptoms of UC and CD. In the Wistar rat model used in the experiment, CD was induced with 2,4,6-trinitrobenzenesulfonic acid, and UC was induced with oxazolone. Clinical status was assessed using the Disease activity index (DAI). Lipid peroxidation (LPO) products were measured in the heptane and isopropanol phases of the intestinal mucosa extract. POM products were measured following spontaneous and stimulated oxidation. The DAI ( Me (Q25–Q75)) was increased in both CD and UC on days 3 and 7 of the experiment: for CD, it was equally increased on days 3 and 7 (7 (3-7)) and was 11 (11–11) and 11 (9–11) for UC on days 3 and 7, respectively. The amount of primary, secondary and end LPO products in the heptane and isopropanol phases, as well as the total amount of POM products, was increased in the homogenized mucosa of the large bowel. In the CD group, the relative content of secondary basic POM products was increased on day 7 of the experiment. The following patterns of FRO were revealed: accumulation of LPO products in the UC group and accumulation of POM products in the CD group; UC is characterized by the accumulation of mostly LPO products in the heptane phase and secondary LPO products in the isopropanol phase; CD is characterized by the accumulation of secondary basic POM products. DAI scores were correlated with the amount of LPO products in the isopropanol phase and the amount of POM products in the spontaneous oxidation mode. The highest number of strong correlations was observed in the UC group. Our findings suggest a very serious contribution of FRO changes to the pathogenesis of UC and CD, meaning that LPO and POM products could be regarded as diagnostic markers and indicators of treatment efficacy.

scholarly journals Limited proteolysis and oxidative modification of proteins in the hepatocytes of patients with resistant forms of tuberculosis

Pneumologia ◽  
2019 ◽  
Vol 68 (3) ◽  
pp. 126-129
Author(s):  
Lilia Todoriko ◽  
Ihor Semianiv ◽  
Radu Criṣan-Dabija ◽  
Olʼha S. Shevchenko
Keyword(s):  
Free Radical ◽  
Protein Modification ◽  
Comparison Group ◽  
Limited Proteolysis ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Bromphenol Blue ◽  
Oxidative Modification Of Proteins ◽  
Pulmonary Tb ◽  
Oxidative Protein Modification

Abstract The aim of this paper was to evaluate the oxidative modification of proteins and limited proteolysis in the hepatocytes of patients with resistant forms of pulmonary tuberculosis (TB). Material and method: This is a prospective pathomorphological study of 60 patients with confirmed pulmonary TB (clinically and anatomopathologically) deaths from various causes. To evaluate the intensity of free radical processes in hepatocytes, we used a methodology developed by Davydenko IS, 2003. Histological sections were stained with bromphenol blue by Michel-Calvo. Results: When comparing the digital data indicated in our study, it is evident that changes in proteins properties in average tendencies run virtually identically in the hepatocytes of different zones of the comparison group. Thus, the difference is unlikely (p > 0.05) when comparing red/blue ratio (specific staining of acid and basic proteins by Michel-Calvo) in the hepatocytes of zones I, II and III of the comparison group. A similar trend is observed in the analysis of average values of the optical density of a specific colouration on the free amino group of proteins by A. Yasuma and T. Ichikava. The analysis of all parameters showed a linear increase in the intensity of oxidative protein modification and limited proteolysis from zone I to III (from the periportal to the centrolobular parts of the acinus) in all subgroups of the main group according to the acinus zone (by Rappoport). Conclusions: Pulmonary TB leads to increased processes of free radical oxidation of proteins with characteristic effects – increased proteolysis and oxidation of amino groups of proteins – in the hepatocytes of the liver.

scholarly journals Histochemical Study of the Processes of Protein Oxidative Modification and Limited Proteolysis in the Endothelium of Myometrial Vessels in the Projection of the Utero-Placental Bed during Iron-Deficiency Anemia in Pregnancy

2021 ◽  
Vol 6 (4) ◽  
pp. 58-63
Author(s):  
O. A. Tiulienieva ◽  
Keyword(s):  
Endothelial Dysfunction ◽  
Iron Deficiency ◽  
Pregnant Women ◽  
Iron Deficiency Anemia ◽  
Limited Proteolysis ◽  
Oxidative Modification ◽  
Deficiency Anemia ◽  
Bromophenol Blue ◽  
Oxidative Modification Of Proteins ◽  
Protein Oxidative Modification

Utero-placental bed is the cumulation of gestationally altered endometrium at the place of ovum attachment to the uterine wall. As far as the protein oxidative modification and limited proteolysis in iron deficiency anemia are due to the fact that in conditions of hypoxia, free radical processes in the blood and tissues are enhanced, and iron deficiency is additionally able to cause hemodynamic disorders because of endothelial dysfunction in the vessels of the utero-placental area. The purpose of the study was to establish histochemical features of protein oxidative modification and limited proteolysis in the endotheliocytes of myometrial vessels in the projection of the utero-placental area depending on the degree of iron deficiency anemia in pregnant women. Materials and methods. By histochemical methods of Mikel Calvo, using reactions with bromophenol blue on "acidic" and "basic" proteins, and the method of A. Yasumа and T. Ichikawa, ninhydrin-Schiff reaction to free amino groups of proteins to assess the degree of limited proteolysis, in combination with computer microspectrophotometry and microdensitometry, quantitative characteristics of oxidative modification of proteins and limited proteolysis in endotheliocytes of myometrial segments of the utero-placental vessels in iron deficiency anemia of pregnant women were established. 74 biopsies of the observed uterine-placental area in physiological pregnancy and gestation based on iron deficiency anemia of I, II and III degrees of severity were investigated. Results and discussion. In physiological pregnancy, the intensity of oxidative modification of proteins and limited proteolysis is the lowest in endotheliocytes of myometrial segments of the spiral arteries and the highest one is in the endothelium of the vessels of the microcirculatory tract of the utero-placental area. In gestations based on iron deficiency anemia, intensification of proteins oxidative modification and limited proteolysis in the endothelium of all types of myometrial vessels of the projection of the utero-placental bed correlates with the severity of anemia. Iron deficiency anemia greatly affects the modification of proteins in the endothelial cells mostly of myometrial segments of spiral arteries of the placental bed. Conclusion. Intensification of protein oxidative modification and limited proteolysis in endotheliocytes of all type vessels of myometrium of the uterine-placental area formed by iron deficiency can be considered as a significant factor of endothelial dysfunction and a predictor of hemodynamic disorders of the placental bed

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