scholarly journals Chronophered approach to appointment of patients with alergodromatosis of antioxidant drugs

2019 ◽  
pp. 11-16
Author(s):  
Ольга Денисенко
Keyword(s):  
Atopic Dermatitis ◽  
Reduced Glutathione ◽  
Clinical Manifestations ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Complex Treatment ◽  
Daily Rhythms ◽  
Oxidative Modification Of Proteins ◽  
Radical Oxidation ◽  
Oxidation Processes

Objective - to increase the efficiency of treatment of pa­tients with allergic dermatitis by using chronodermined antioxidant drugs, taking into account the daily rhythms of the parameters of prooxidant antioxidant homeostasis. Materials and methods: 73 patients with allergic derma­titis (eczema, allergic and atopic dermatitis) were exam­ined, which determined the circadian (daily) rhythms of the parameters of prooxidant-antioxidant homeostasis. Results and discussion. In patients with allergic der­matitis, an increase in the mesor and amplitude of ma­lonic aldehyde, the fractions of oxidative modification of proteins and mediomolecular peptides with acrophase in the evening hours, as well as the reduction of the mesor of reduced glutathione and ceruloplasmin with batifase in evening hours, has been established, indicating activa­tion and growth during free radical oxidation processes lipids and proteins against the background of a decrease in the activity of antioxidant flora, which substantiates the chronoderminable purpose of such a patient with antioxidant drugs. Conclusion: Chronodetermined ap­pointment in the complex treatment of allergic dermati­tis antioxidant drugs accelerates the regression of their clinical manifestations, reduces the timing of treatment of patients, as well as helps to normalize their prooxidant antioxidant homeostasis.

scholarly journals Окиснювальна модифікація білків у щурів різного віку за умов гострого отруєння токсинами блідої поганки

2017 ◽  
Author(s):  
I. P. Kuzmak ◽  
Ye. B. Dmukhalska ◽  
S. R. Pydruchna ◽  
T. Ya Yaroshenko ◽  
I. Ya. Krynytska ◽  
...  
Keyword(s):  
Age Groups ◽  
Intraperitoneal Administration ◽  
Free Radical Oxidation ◽  
Numerical Data ◽  
Pathological Process ◽  
Oxidative Modification ◽  
Amanita Phalloides ◽  
Oxidative Modification Of Proteins ◽  
Radical Oxidation ◽  
Protein Oxidative Modification

Introduction. In recent years, scientific articles of domestic and foreign scientists obtained numerical data on the important role of oxidative modification of proteins in the development of many diseases. It is known that entering the organism of toxic compounds, including toxins of Amanita phalloides, sharply oppress antioxidant system increases the contents of peroxidation products lipids and proteins in liver, kidneys.The aim of the study – to examine the dynamics of oxidative modification of proteins in rats of different ages affected by poison Amanita phalloides toxins in different periods of the experiment.Methods of the research. In all experimental groups poisoning was modeled by single intraperitoneal administration Amanita phalloides extract obtained by the method of H. Wieland (85 mg / kg body weight). Euthanasia was performed under sodium thiopental anesthesia at 6, 24 and 72 hours after poisoning. Oxidative modification of proteins in blood serum was investigated by Meschyshen method.Results and Discussion. Protein oxidative modification indices in all age groups rats on the model of toxic injury by Amanita phalloides toxins were investigated. It was found out that the progression of the pathological process in the liver and the depletion of the compensatory capacity in animals with acute poisoning by Amanita phalloides leads to the activation of free radical oxidation of proteins and cause the increasing of protein oxidative modification indices. The most pronounced changes occur in 6- and 18- month rats in 24 hours after intoxication.Conclusions. It was defined, that the progression of the pathological process in the liver and the depletion of the compensatory capacity as a result of poisoning by toxins of Amanita phalloides cause the increasing of protein oxidative modification indices. It can be considered as one of the earliest markers of damage to organs and tissues including hepatocytes and reliable early predictor of the development and pathology related oxidative stress.

scholarly journals Effect of hypoxia tolerance on the relation between indicators of free radical oxidation of lipides and proteins in murine kidneys during the post-resuscitation period

2017 ◽  
Vol 98 (6) ◽  
pp. 949-954 ◽  
Author(s):  
G A Bayburina ◽  
E A Nurgaleeva ◽  
E F Agletdinov ◽  
A F Samigullina
Keyword(s):  
Free Radical ◽  
Free Radical Oxidation ◽  
Systemic Circulation ◽  
Oxidative Modification ◽  
Hypoxia Tolerance ◽  
Oxidative Modification Of Proteins ◽  
Radical Oxidation ◽  
Resistance To Hypoxia ◽  
The Relationship ◽  
Observation Period

Aim. Study of the relationship between the parameters of free radical oxidation of proteins and lipids in the murine kidneys in the post-resuscitation period after stopping the systemic circulation, depending on their resistance to hypoxia. Methods. The systemic circulation was stopped by intra-thoracic clamping of the neurovascular bundle for 5 minutes, performed under general ether anesthesia in male noninbred white rats, divided after testing into two groups based on resistance to hypoxia. The observation period lasted for 35 days. In the homogenates of kidney tissues, the content of products reactive to tiobarbituric acid, carbonylated proteins, the formation of metal-catalyzed carbonylated proteins and bitirozin were determined. Results. The characteristic manifestation of oxidative stress in the recovery period after stopping blood circulation and resuscitation was found to be reciprocity of the relationship between the levels of lipoperoxidation and oxidative modification of proteins. Highly resistant to hypoxia animals were characterized by high resistance of proteins of kidney tissue to free radical oxidation against the background of high levels of lipid peroxidation. On the contrary, in animals non-resistant to hypoxia, against the background of relatively low values of lipoperoxidation, high levels of oxidative modification of proteins, both initial and induced, were recorded. Conclusion. In post-resuscitation period in highly resistant to hypoxia animals, marked activation of lipoperoxidation occurs accompanied by a transient increase in the carbonylation of proteins in the early observation period; for low-resistant to hypoxia animals high intensity of carbonyl stress against the background of the relative «preservation» of lipid structures of the cell is characteristic, which persists throughout the post-resuscitation period, which can make a significant contribution to kidney damage, increasing the risk of renal failure.

Clinical and pathogenetic role of state of prooxidant-antioxidant regulation in patients with purulent meningitis in the dynamics of disease

2014 ◽  
Vol 18 (4 (72)) ◽  
Author(s):  
D. A. Zadyraka ◽  
E. V. Riabokon
Keyword(s):  
Free Radical ◽  
Conventional Treatment ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Blood Proteins ◽  
Radical Oxidation ◽  
Pathogenetic Role ◽  
Purulent Meningitis ◽  
Laboratory Changes

The paper shows, that patients with purulent meningitis on the background of severe clinical and laboratory changes had a disturbance of prooxidant-antioxidant regulation with a shift towards activation of free radical oxidation. In the dynamics of the disease, after 7-10 days of conventional treatment, against the background of a reduction of cephalic and meningeal syndromes intensity, a normalization of liquor and hemogram indices, in most patients there was a further decrease in catalase activity and an increase of nitrites, of parameters of spontaneous and induced oxidative modification of blood proteins that remained when the patients were discharged, compared with healthy people.

scholarly journals Influence of neuropeptides acth(4-7)-pro-gly-pro and acth(6-9)-pro-gly-pro on the intensity of redox reactions under experimental depression

2020 ◽  
Vol 10 (3) ◽  
pp. 29-32
Author(s):  
Marina Samotrueva ◽  
Anna Yasenyavskaya ◽  
Aleksandra Tsibizova ◽  
Jumazia Erizhepova ◽  
Nikolai Myasoedov ◽  
...  
Keyword(s):  
Free Radical ◽  
Social Stress ◽  
Redox Reactions ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
Male Rats ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Initial Content ◽  
The Brain

The experiment is devoted to the study of the antioxidant properties of neuropeptides from melanocortins ACTH(4-7)-Pro-Gly-Pro (Semax) and ACTH(6-9)-ProGly-Pro under conditions of experimental depression. The study was carried out on white outbred male rats. In the process of modeling experimental depression (social stress) inter-male confrontations were observed as a result of which groups of animals with aggressive and submissive behaviors were formed. The free radical oxidation processes were assessed by determining the activity of catalase, the initial content of malondialdehyde (MDA), the rate of spontaneous and ascorbate-dependent lipid peroxidation (LPO) in the hypothalamic and prefrontal regions of the brain by spectrophotometric method. It was found that under the influence of melanocortins, there is a pronounced suppression of the processes of free radical oxidation in the hypothalamic and prefrontal regions of the brain, which arose against the background of a stressful load which is manifested by a decrease in the indicators of the oxidative process.

scholarly journals Genetic peculiarities of free radical oxidation of lipids and proteins in the semen of breeding boars

Biologija ◽  
2018 ◽  
Vol 64 (3) ◽  
Author(s):  
Svitlana Polishchuk ◽  
Svitlana Tsekhmistrenko ◽  
Vitalii Polishchuk ◽  
Oksana Tsekhmistrenko ◽  
Nataliia Ponomarenko ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Reproductive Function ◽  
Oxidative Modification ◽  
Peroxide Oxidation ◽  
Large White ◽  
Radical Oxidation ◽  
Key Enzyme ◽  
Synthetic Line

Complex research into biochemical aspects of the functioning of the antioxidant system for the protection of sperm of the breeding boars has established that the intensity of the flow of peroxide oxidation of lipids in germ cells is much higher than in the extracellular space. Reactions of free radical peroxide oxidation of proteins and lipids in the semen of healthy breeding boars are characterized by a stable level of activity that is necessary for the normal course of implementation of processes of the reproductive function. All components of the antioxidant system stay in mutual compensatory ratios under physiological conditions. As a rule, reducing the concentration or activity of some antioxidant enzymes leads to corresponding changes in others. The main antioxidants of the germ cell genomes are superoxide dismutase and ceruloplasmin. Catalase is a key enzyme that neutralizes H2O2 in the semen fluid of breeding boars. The content of total proteins in the semen of the synthetic line SS23 animals is greater (p < 0.05) than in the breeding boars of the large white breed. The processes of the oxidative modification of proteins in the animals body of the synthetic line runs more intensely, as it is evidenced by the higher content of the aldehyde and ketodinitrophenyl hydrazones of the main and neutral character in the sperm cytoplasm.

scholarly journals CHARACTERISTIC OF FREE-RADICAL DAMAGE AND ANTIOXIDANT PROTECTION STATE IN PATIENTS WITH CHRONIC KIDNEY DISEASE AGAINST A BACKGROUND OF MALABSORPTION SYNDROME

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
А.Е. Shkarutyak
Keyword(s):  
Chronic Kidney Disease ◽  
Free Radical ◽  
Kidney Disease ◽  
Free Radical Oxidation ◽  
Oxidation Products ◽  
Essential Factor ◽  
Free Radical Damage ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Radical Damage

Oxidation processes play an important role in damage of the renal structures, especiallyagainst a background of other serious pathologies, in particular, malabsorptionsyndrome.The study of the mechanisms of mutual aggravation and progression of kidneydamage against a background of malabsorption is a very urgent problem of the presenttime.Purpose - to investigate the state of free radical oxidation of lipids, proteins andantioxidant defense in patients with chronic kidney disease of different ages withoxaluria against a background of malabsorption syndrome.Material and methods. 98 patients with chronic kidney disease of the I-II stages(pyelonephritis) with the presence of oxaluria against a background of malabsorptionsyndrome of different age were examined.The age of the patients under examination was32 - 64. All patients underwent a study of the system of free radical oxidation of lipidsand proteins.Results. chronic kidney disease with the presence of oxaluria is accompanied by asignificant increase in the blood content of free radical oxidation products, namelyaldehyde and ketone dinitrophenylhydrazones, especially of a neutral nature,malondialdehyde, as well as a significant decrease in the indicators of the antioxidantdefense system, which manifested itself to a greater extent in patients with older age.groups.Conclusion. An essential factor in the development and progression of chronic kidneydisease with the presence of oxaluria against a background of malabsorption syndromeis an increase in the intensity of free radical oxidation of lipids and proteins (mainly dueto aldehyde and ketondinitrophenylhydrazones of a neutral nature).

scholarly journals INFLUENCE OF LASER TREATMENT ON INDICATORS OF FREE-RADICAL OXIDATION AND ANTIOXIDANT PROTECTION IN SECONDARY PYELONEPHRITIS

2019 ◽  
Vol 19 (1-2) ◽  
pp. 149-156
Author(s):  
I. I Antipkin ◽  
E. V Ryazantsev ◽  
V. E Ryazantsev ◽  
A. M Khoshnaw
Keyword(s):  
Catalase Activity ◽  
Clinical Medicine ◽  
Malonic Dialdehyde ◽  
Free Radical Oxidation ◽  
The Body ◽  
Complex Treatment ◽  
Metabolic Homeostasis ◽  
Radical Oxidation ◽  
Non Destructive ◽  
Single Kidney

Relevance. Optimization of the conservative tactics of acute inflammation of the kidney in nephrolithiasis makes it advisable to use non-traditional methods of detoxification low-intensity laser radiation (LILR) in particular. Aim. To evaluate the effectiveness of laser therapy (LT) in the complex correction of acute secondary pyelonephritis (ASP) in patients with single kidney stones according to the basic adaptation criteria of oxidative-antioxidant clinical medicine КЛИНИЧЕСКАЯ МЕДИЦИНА homeostasis of the organism. Materials and methods. Indices of endogenous intoxication (EI), characterizing the oxidative-antioxidant homeostasis of the body in ASP, were studied in 96 patients with nephrolithiasis aged 19 to 40 years. Patients of the comparison group (48 people) underwent basic (according to the standards) treatment aimed at controlling of calculous pyelonephritis. Patients of the main group (48 people) in the traditional conservative therapy from the second day of hospitalization daily received additional LT sessions with apparatus LTA “Uzor-3KS” with a frequency of 0.6 kHz, a power of 1.5 watts within 4 minutes (total for treatment course is eight procedures). The effectiveness of therapy was assessed on the 5th and 9th day of the inpatient stay. Results. It was found that endotoxicosis in non-destructive forms of ASP is caused by the increase in the concentration of malonic dialdehyde of plasma (by 20.3%) and erythrocytes (by 35.5%), decrease in catalase activity (by 14.2%) and the amount of total (by 11.8%) and free (21.1%) thiol groups with a decrease in the detoxification index (DI) by 24,1%. Compared with the baseline therapy (BT), the effectiveness of LT in the complex treatment of ASP in the analyzed parameters of metabolic homeostasis against the background of the increase in catalase capacity from 6.9 to 10.2%, the detoxification index from 5 to 12.5% was 7.5% (on the 5th day) and 17.5% (on the 9th day of treatment). Conclusions. ASP leads to activation of ROS, suppression of antioxidant and catalase activity of blood, reduction of DI, indicating a subcompensated syndrome of EI. Standard therapy contributes to the normalization of ROS and SLA values of body protection and reduces the level of endotoxicosis caused by ASP. Additional inclusion in the complex treatment of LT sessions more significantly normalizes redox metabolic homeostasis and EI parameters, improving the effectiveness of basic therapy.

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