Assessment of the immunological status in patients of a surgical hospital

1993 ◽  
Vol 74 (3) ◽  
pp. 218-219
Author(s):  
I. V. Klyushkin ◽  
E. E. Krasnoshchekova ◽  
K. T. Valeeva ◽  
M. N. Nasrullaev

In patients with a surgical profile, the humoral link of the immune system was studied for the production of 3 classes of immunoglobulins (IgA, IgM, IgG), the formation of circulating immune complexes (CIC), bactericidal activity of blood serum (BASK) in comparison with the clinical picture of the disease. Serum immunoglobulins were determined according to Mancini, CEC by the conventional method according to D. K. Novikov (1987), BASK by the method of V. I. Nikitenko and O. V. Bukharin.

2019 ◽  
Vol 21 (94) ◽  
pp. 132-135
Author(s):  
Y. V. Duda

One of the actual rabbits breeding problem is the reduction of their resistance, which is caused by the spread of individual invasive diseases, especially cysticercosis, which is caused by the larval Cysticercus pisiformis stage of the mature cestode Taenia pisiformis. The study was conducted on 38 male rabbits age 3–4 months, weighing 3.5–4.0 kg of the Californian breed, selected by analogy. Animal were separated into two groups: healthy animals (control group) and sick animals (research group). After blood collection, slaughter was conducted and the presence of cysticercus cysts was visually determined in the organisms of the rabbits. Control animals received a balanced standard granular feed and water without limit; research group in addition to the standard granulated feed with water received flaccid hay. The animals were kept in mesh single-tier cages in the room, according to the current veterinary and sanitary standards. The definition of phagocytic activity of neutrophils was carried out with the addition of standardized to 2500000000/ml suspension of daily culture of E. coli 055K59№3912/41. The bactericidal activity of blood serum was determined by the method Smirnova A.V. and Kuzmina T.A. in relation to the E. coli microbial test-culture 055K59№3912/41. The serum lysozyme activity was determined by the Nephelometric method using the Dorofachuk V.G. method to the microbial test culture Micrococcus luteus ATSS9341. Circulating immune complexes were determined using polyethylene glycol in borate buffer (pH 8.4). It was found that in blood of sick animals phagocytic activity is lower than in blood of healthy ones (respectively, 41.82 ± 1.51% versus 47.38 ± 1.10%, P < 0.01). The main cells involved in phagocytosis are leukocytes, in particular neutrophils and eosinophils. A low indicator of phagocytic activity shows depressed phagocytosis in the organism of animals suffering from cysticercosis of rabbits. The phagocytic number in the blood of rabbits of the experimental group was significantly lower by 0.51 units (P < 0.05) as compared to the control group and correlated with the index of phagocytic activity. An important element of immunity are indicators of bactericidal and lysozyme activity of blood serum. Low bactericidal activity of serum by 12.88% (P < 0.01) and lysozyme activity in rabbits with Cysticercus pisiformis also indicates a weakening of the factors of nonspecific natural resistance of the organism. Analyzing the level of circulating immune complexes, we found a high level of medium (9.14 ± 0.40 vs 5.58 ± 0.50) and small (9.21 ± 1.35 vs 4.64 ± 0.68) CIC for cysticercosis, respectively, 1.64 (P < 0.01) and 1.98 times (P < 0.001) against the control. This indicates the inhibition of the immunobiological activity in the organism of rabbits as a result of the combination of specific antibodies with the products of the exchange of helminths.


2018 ◽  
Vol 20 (87) ◽  
pp. 32-37 ◽  
Author(s):  
V.G. Stoyanovskyy ◽  
A.О. Krogh ◽  
I.A. Kolomiіets

The article deals with the question of adaptation of the ducks organism during industrial cultivation for action of stress. The complex of successive stage reactions, caused by the functioning of stress-implementing systems, arise in the body of the poultry in response to the influence of destabilizing factors and aimed at neutralizing these factors, preservation of homeostasis. Increasing the influence of technological stress factors leads to a decrease in the immune status of the duck organism. The study of the effectiveness of incorporating into the diet ducks harmless feed additives, which contributes to the normalization of the course of adaptive-compensatory reactions in their body during the critical periods of postnatal ontogenesis and can serve as anti-stress prevention in conditions of industrial growth of this species of poultry remains relevant. The purpose of the work is to find out the implementation of an adaptation syndrome in the system of indices of non-specific resistance of the body of ducks when it is included in the diet of the biologically active feed supplement «Primix Bionorm K» and supplements «Biovir». The experiment was conducted on clinically healthy young ducks of the Pekin breed of egg production line. In the 270-day age, all three groups of ducks were modeled experimental technological stress in the form of prolonged transport. From 240 to 270 days of age, ducks additionally fed the additive «Primix Bionorm K» and was pouring with water an additive «Biovir». The material for research was blood, which was taken until morning feeding after slaughter on 240 days of life (to stress), 270 days of life (stage of anxiety), at 273 and 285 days (stages of resistance). Adaptation of the state of non-specific resistance of the duck of the control group to the control group under conditions of stress in the anxiety stage is characterized by an increase of 17.3% (Р < 0.05) of the value of the bactericidal activity of the blood serum, a decrease of 8.5% of the value of lysozyme activity of the blood serum, by 9.3% of phagocytic activity against the background of growth on 8.7% of the number of circulating immune complexes compared to 240-day-old poultry. At the stage of resistance there is a decrease in the amount of blood serum bactericidal activity by 17.3% (Р < 0.05), an increase in the activity of lysozyme in blood serum by 8.5%, and the number of immune complexes by 20.7% (Р < 0.05), with the stabilization of the studied parameters at the later stages of the development of a stress reaction. In these periods, a decrease in the body weight of control group ducks was set at 5.7 – 6.8%. Feeding of the additive «Primix Bionorm K» during the period of the implementation of the stress syndrome has a positive effect on the indices of the cellular and humoral level of nonspecific resistance in the form of an increase in the amount of bactericidal activity of the serum on 38.2% (P < 0.05), an increase in the activity of phagocytosis by 15.1% (P < 0.05), phagocytic index – by 36.1% (P < 0.05) with a decrease in the number of circulating immune complexes by 15.5%. The presentation of the additive «Biovir» causes an increase in the level of bactericidal and lysozyme activity of the blood serum on 47.3% (P < 0.01) and 18.7% (P < 0.05). The use of additives can reduce the negative effect of transport stress in the duck body, increase nutrient uptake, as indicated by an increase in the body weight of experimental birds in the average by 7.1–10.5% (P < 0.05) during the development of the adaptive syndrome.


2018 ◽  
Vol 20 (88) ◽  
pp. 136-140 ◽  
Author(s):  
V. V. Zhurenko ◽  
O. V. Zhurenko

Cows, young cattle and calves of black-and-bark breeds, spontaneously affected by the causative agent Cryptosporidium parvum, were selected for the research. Blood for research was taken from calves in the morning before feeding from the jugular vein. Biochemical parameters of blood serum were determined using a biochemical analyzer of the closed type. Changes in the activity of serum enzymes in the initial stages of the disease are not specific, they indicate a compensatory reaction of liver cells and biliary tract. Effect of Cryptosporidium in calves body accompanied by severe disorders that affect the metabolism of proteins, carbohydrates and enzymes. These changes lead to structural changes in the organs at the cellular level. Serum infested animals significantly reduced total protein content of 25.5% (P < 0.001), albumin – by 14.6% (P < 0.01), the concentration of glucose in – 21.2% (P < 0.01), carotene content – 12.6% (P < 0.05), the level of calcium and phosphorus – 17.5 and 18% (P < 0.05), respectively, and increased total bilirubin content by 23% (P < 0.001), indicating significant violations of the protein synthesis and detoxification processes in the liver and the tension in the metabolism of their body. Reduced glucose concentration in blood serum is due to the fact that in the body of sick animals there have been increased costs for maintaining the energy needs of its own organism. In conducting studies for 35 days in animals in the experimental group noted an increase in the concentration of circulating immune complexes by 10.5% relative to control. Increasing the concentration of circulating immune complexes in serum of calves, patients with cryptosporidiosis, indicates the presence of specific interaction antigen-antibody and decrease the activity of the humoral link of the immune system. In the determination of immunosuppressive proteins – serum cords in serum of calves, it was found that at 7, 14 days their concentration was within the physiological limits. Increasing the concentration of circulating immune complexes in serum of calves, patients with cryptosporidiosis, indicates the presence of specific interaction antigen-antibody and decrease the activity of the humoral link of the immune system.


2009 ◽  
Vol 8 (4) ◽  
pp. 148-151
Author(s):  
V. S. Dmitruk ◽  
L. V. Striga

The influence of the chronopharmacological scheme of treatment with the use of exogenous Melatonin in the form of the Melaksen drug on the immunity status of psoriasis patients has been studied. The study of the immunity status consisted in determination of the main Manchini classes of immunoglobulins A, M, G, the level of circulating immune complexes by the method of selective precipitation in 3% polyethylene glycol. In addition, we determined the subpopulation of lymphocytes carrying CD3, CD4, CD8, CD16, CD 25, and CD72 antigens by the immunofluorescent method. In has been found that Melatonin in psoriasis patients exerts the immunomodulatory action on the immune system at the combined treatment (due to an increase in the number of -lymphocytes with cytotoxic-suppressive properties at obvious signs of immune disbalance in the form of the increased ratio of Т CD4+ and CD8+).


2019 ◽  
Vol 10 (1) ◽  
pp. 87-91
Author(s):  
A. R. Shcherbatyy ◽  
L. G. Slivinska ◽  
B. V. Gutyj ◽  
V. L. Fedorovych ◽  
B. O. Lukashchuk

We observed the processes of lipid peroxidation, characterized by increased content of intermediate and its end products (diene conjugates, lipid hydroperoxides, malonic dialdehyde), in the blood of the pregnant mares. The changes of the data of indicators in the organism of the mares at the 9–11 months of pregnancy were established. The level of diene conjugates in the blood of animals was 3.6 ± 0.12 μmol/l, 33.3 and 44.0%, which was higher compared to non-pregnant mares and mares at 4th month of pregnancy. The concentration of lipid hydroperoxides in the blood of mares increased by 100% and by 42.9% in the blood of non-pregnant mares and mares at 4th month of pregnancy. The concentration of malondialdehyde in the blood of mares increased by 75.0%, 51.2% and 25.0% compared to non-pregnant mares and mares at 4th and 7th month of pregnancy, respectively. The results of our research showed that the mineral-vitamin premix Marmix had a positive influence on the state of lipid peroxidation products, contributed to their lowering in the blood of pregnant mares, which demonstrated antioxidant action and stimulated the function of the immune system. It was established that the bactericidal and lysozyme activity of the blood serum and the concentration of circulating immune complexes increases in the mares with lack of trace elements. It was shown that feeding mares during the 9–11 months of pregnancy with the mineral-vitamin premix Marmix had a positive influence on the state of humoral factors of nonspecific resistance. In particular, the concentration of bactericidal activity of serum increased by 31.0%, lysozyme activity of blood serum – 45.4% and contributed to the decrease of the concentration of circulating immune complexes in serum by 3.8 times. The use of mineral-vitamin premix Marmix during 60 days in feeding pregnant mares caused a restoration of the clinical status, a decrease in the content of lipid peroxidation products.


Author(s):  
M. S. Reheda ◽  
L. A. Lubinets ◽  
B. F. Shchepanskyi

In this paper, it is shown that the modeling process of bronchial asthma (BA ) is accompanied by changes, in comparison with the control group, in the indicators of immune system response: T- & B-lymphocytes, circulating immune complexes in blood of guinea pig males on the 4th, 18th, 25th day of experiment.The aim of the study – determination of some indices of immune system in blood of guinea pigs in the modeling process of BA on 4th, 18th, 25th day of the experiment.Materials and Methods. Experiments were conducted on 40 guinea pigs (males), with body weight 0.25–0.27 kg. Animals were divided into four groups of ten animals in each. Intact guinea pigs were the first group. Animals with experimental BA – the second, third, fourth group respectively on the 4th, 18th, 25th day of the experiment. Experimental BA was reproduced by V. I. Babych method. In blood of intact guinea pigs and animals with experimental BA , the number of T- and B-lymphocytes was determined by the method of E. F. Chernushenko, L. S. Kohosov, determination of the level of circulating immune complexes was carried out by the method of V. Haskova and co-authors. The results of the study were processed by the method of variation statistics using Student's criterion.Results and Discussion. The results of the studies showed unidirectional changes in individual parameters of the immune system, depending on the periods of the formation of BA : an increase in the number of B-lymphocytes and circulating immune complexes, a decrease in the number of T-lymphocytes for all of the studied days of the experiment.Conclusions. The obtained results indicate significant changes in the immune system parameters in the blood of experimental animals with BA and are important for understanding the pathogenesis of BA . These studies provide an opportunity to find the more perfect and effective methods of diagnosis of BA.


2021 ◽  
Vol 102 (5) ◽  
pp. 626-635
Author(s):  
A U Sabitov ◽  
D A Soldatov ◽  
Yu B Khamanova

Aim. To assess the clinical and immunological features in patients with chronic opisthorchiasis, depending on the duration of the infection. Methods. The first group consisted of 19 patients with the duration of the infection up to 1 year, the second group consisted of 21 patients with the duration of the infection between 1 and 5 years, the third group was formed of 23 patients with the duration of the disease more than 5 years, the control group 20 healthy individuals. Immunological research was carried out at the Clinical Diagnostic Center. Statistical processing was performed using Microsoft Excel 2010 and Statistica 6.0 software. The statistical significance of differences was determined by using the MannWhitney test (U-test) at the level of significance of p 0.05. The correlations were assessed by calculating Spearman's rank correlation coefficients.Results Clinical features of chronic opisthorchiasis were revealed in the disease duration groups of up to 1 year, from 1 year to 5 years, more than 5 years: the subclinical course was most common in the group of up to 1 year; cholangiohepatitis prevailed in the group of between 1 to 5 years, allergic skin syndrome, cholangiocholecystitis and pancreatitis dominated in the group of more than 5 years. The immune response in chronic opisthorchiasis was characterized by: up to 1 year lymphocytosis, increased levels of immunoglobulins M (IgM) and circulating immune complexes (CIC), a decrease in the number of T-lymphocytes (CD3+), as well as an increase in bactericidal activity of leukocytes (BAL); between 1 and 5 years monocytosis, increased levels of immunoglobulins M, immunoglobulins G and circulating immune complexes, a decrease in T-cytotoxic lymphocytes (CD8+) and nitro blue tetrazolium (NBT test), as well as an increase in NK cells and phagocytic activity of monocytes, more than 5 years eosinophilia. Conclusion. Common features of rearrangement of the immune system in opisthorchiasis: inflammatory changes in the hemogram, activation of humoral immunity with parallel suppression of the cellular component of the immune system, and increased phagocytosis.


2020 ◽  
Vol 3 ◽  
pp. 135-138
Author(s):  
O. V. Kryachko ◽  
◽  
L.A. Lukoyanova ◽  

A small amount of circulating immune complexes is determined in a healthy body, and it naturally increases when there is an antigen in the circulation. Stressful situations can inhibit the implementation of immune protection, both adaptive and innate immune responses. The purpose of this study was to study the dynamics of the level of circulating immune complexes in blood serum after modeling single and combined stress effects in rabbits. 10 male rabbits were used for the experiment. The animals were 8 months old and had a live weight of 3.40±0.73 kg. Transport stress was caused by transporting animals in a car. Immobilization stress was modeled by fixing rabbits in a fixation de-vice for small animals by their legs in the dorsal position. In the final series of experi-ments, a combination of actions was per-formed (immobilization, then, after three days, transportation). As a result of studies it was found that the reaction of animals to stress was characterized by decreased level of the CIC, during transportation (by 1.2 times (p<0.05) and immobilization (1.6 times, p<0.05), the combination of stress factors showed a similar effect. In the post-stress period, we observed in all cases an increase in the CIC level in the blood of rab-bits, which exceeded the CIC values both at the time of the stress reaction and the level of intact animals.


Biology ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 123
Author(s):  
Elena Velichko ◽  
Sergey Makarov ◽  
Elina Nepomnyashchaya ◽  
Ge Dong

Determination of the concentration and size of the circulating immune complexes in the blood is an essential part of diagnostics of immune diseases. In this work, we suggest using the dynamic light scattering method to determine the sizes of circulating immune complexes in blood serum. By the dynamic light scattering spectrometer, we found that for healthy and sick donors, the size and concentration of circulating immune complexes differed significantly. The dynamics of formation of these complexes were also examined in this work. It was shown that the formation of immune complexes in the blood of healthy donors is faster than the same reactions in the blood serum of donors with diseases. The results can be used in the diagnostics of the immune status and detection of chronic inflammation. We can recommend the dynamic light scattering method for implementation in biomedical diagnostics.


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