scholarly journals Estimation of proinflammatory cytokine and heat shock protein levels in atopic dermatitis

2019 ◽  
Vol 36 (3) ◽  
pp. 38-42
Author(s):  
Natalia M. Ivanova ◽  
Irina V. Kibalina ◽  
Namzil N. Tsybikov
Keyword(s):  
Atopic Dermatitis ◽  
Stress State ◽  
Proinflammatory Cytokine ◽  
Statistical Processing ◽  
Hsp 70 ◽  
Hsp70 Level ◽  
Clinical Symptomatology ◽  
Protein Levels ◽  
Inflammatory Damage ◽  
Long Time

Aim. To estimate the proinflammatory cytokineI L-17α level, HSP-70 level and autoantibodies to it in pathogenesis of atopic dermatitis (AD) at its exacerbation stage. Materials and methods. Twenty patients aged 18–35 years were examined; cytokine IL-17α levels, HSP-70 chaperone levels and autoantibodies to it were studied in patients, suffering from typical form of atopic dermatitis at exacerbation stage. Statistical processing of the obtained material was implemented with STATISTICA 6.1 for Windows. Results. Atopic dermatitis causes chronic inflammatory damage of the skin, the cells are in stress state for a long time that contributes to accumulation of the damaged proteins in them and significant growth of chaperone synthesis. Conclusions. In the period of active clinical symptomatology, patients with atopic dermatitis demonstrated decrease in blood IL-17α concentration and increase in HSP70 level and autoantibodies to it.

scholarly journals Surfactant protein A reduces TLR4 and inflammatory cytokine mRNA levels in neonatal mouse ileum

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lidan Liu ◽  
Chaim Z. Aron ◽  
Cullen M. Grable ◽  
Adrian Robles ◽  
Xiangli Liu ◽  
...  
Keyword(s):  
Proinflammatory Cytokine ◽  
Inflammatory Cytokine ◽  
Protein A ◽  
Surfactant Protein ◽  
Surfactant Protein A ◽  
Mrna Levels ◽  
Wild Type ◽  
Cytokine Mrna ◽  
Protein Levels ◽  
Cytokine Levels

AbstractLevels of intestinal toll-like receptor 4 (TLR4) impact inflammation in the neonatal gastrointestinal tract. While surfactant protein A (SP-A) is known to regulate TLR4 in the lung, it also reduces intestinal damage, TLR4 and inflammation in an experimental model of necrotizing enterocolitis (NEC) in neonatal rats. We hypothesized that SP-A-deficient (SP-A−/−) mice have increased ileal TLR4 and inflammatory cytokine levels compared to wild type mice, impacting intestinal physiology. We found that ileal TLR4 and proinflammatory cytokine levels were significantly higher in infant SP-A−/− mice compared to wild type mice. Gavage of neonatal SP-A−/− mice with purified SP-A reduced ileal TLR4 protein levels. SP-A reduced expression of TLR4 and proinflammatory cytokines in normal human intestinal epithelial cells (FHs74int), suggesting a direct effect. However, incubation of gastrointestinal cell lines with proteasome inhibitors did not abrogate the effect of SP-A on TLR4 protein levels, suggesting that proteasomal degradation is not involved. In a mouse model of experimental NEC, SP-A−/− mice were more susceptible to intestinal stress resembling NEC, while gavage with SP-A significantly decreased ileal damage, TLR4 and proinflammatory cytokine mRNA levels. Our data suggests that SP-A has an extrapulmonary role in the intestinal health of neonatal mice by modulating TLR4 and proinflammatory cytokines mRNA expression in intestinal epithelium.

scholarly journals Siraitia grosvenorii Residual Extract Attenuates Atopic Dermatitis by Regulating Immune Dysfunction and Skin Barrier Abnormality

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3638
Author(s):  
Yoon-Young Sung ◽  
Heung-Joo Yuk ◽  
Won-Kyung Yang ◽  
Seung-Hyung Kim ◽  
Dong-Seon Kim
Keyword(s):  
Atopic Dermatitis ◽  
Immunoglobulin E ◽  
Allergic Inflammation ◽  
Skin Barrier ◽  
Human Keratinocytes ◽  
Skin Lesions ◽  
Protein Levels ◽  
Siraitia Grosvenorii ◽  
Ifn Γ

Atopic dermatitis is a persistent inflammatory skin disorder. Siraitia grosvenorii fruits (monk fruit or nahangwa in Korean, NHG) are used as a natural sweetener and as a traditional medicine for the treatment of asthma and bronchitis. We evaluated the activity of S. grosvenorii residual extract (NHGR) on allergic inflammation of atopic dermatitis in a Dermatophagoides farinae mite antigen extract (DfE)-treated NC/Nga murine model and in vitro. Oral administration of NHGR significantly reduced epidermal hyperplasia and inflammatory cell infiltration in the skin lesions of DfE-induced atopic dermatitis, as well as the dermatitis severity score. NHGR reduced serum immunoglobulin E levels. Splenic concentrations of IFN-γ, interleukin (IL)-4, IL-5, and IL-13 were reduced by NHGR administration. Immunohistofluorescence staining showed that NHGR administration increased the protein levels of claudin-1, SIRT1, and filaggrin in atopic dermatitis skin lesions. In addition, NHGR inhibited the phosphorylation of mitogen-activated protein kinases and decreased filaggrin and chemokine protein expression in TNF-α/IFN-γ-induced human keratinocytes. Moreover, NHGR also inhibited histamine in mast cells. The quantitative analysis of NHGR revealed the presence of grosvenorine, kaempferitrin, and mogrosides. These results demonstrate that NHGR may be an efficient therapeutic agent for the treatment of atopic dermatitis.

Experimental Identification of Model Parameters and the Statistical Processing Using a Nonlinear Oscillator Applied to EEG Analysis

2018 ◽  
Author(s):  
Kenyu Uehara ◽  
Takashi Saito
Keyword(s):  
Stress State ◽  
Nonlinear Oscillator ◽  
Statistical Processing ◽  
Psychological State ◽  
Model Parameters ◽  
Eeg Analysis ◽  
Analysis Model ◽  
Eeg Data ◽  
Machine Interface ◽  
Almost All

The nonlinear analysis may help to reveal the complex behavior of the Electroencephalogram (EEG) signal. In order to analyze the EEG in real time, we have proposed an EEG analysis model using a nonlinear oscillator with one degree of freedom and minimum required parameters. Our method identifies EEG model parameters experimentally. The purpose of this study is to examine the specific characteristic of model parameters. Validation of the method and investigation of characteristic of model parameters were conducted based on alpha frequency EEG data in both relax state and stress state. The results of the parameter identification with the time sliding window for 1 second show almost all of the identified parameters have a normal distribution spread around the average. The model outputs can closely match the complicated experimental EEG data. The results also showed that the existence of nonlinear term in the EEG analysis is crucial and the linearity parameter shows a certain tendency as the nonlinearity increases. Furthermore, the activities of EEG become linear on the mathematical model when suddenly change from the relax state to the stress state. The results indicate that our method may provide useful information in various field including the quantification of human mental or psychological state, diagnosis of brain disease such as epilepsy and design of brain machine interface.

scholarly journals IFNγ-induced suppression of β-catenin signaling: evidence for roles of Akt and 14.3.3ζ

2014 ◽  
Vol 25 (19) ◽  
pp. 2894-2904 ◽  
Author(s):  
Porfirio Nava ◽  
Ryuta Kamekura ◽  
Miguel Quirós ◽  
Oscar Medina-Contreras ◽  
Ross W. Hamilton ◽  
...  
Keyword(s):  
Proinflammatory Cytokine ◽  
Intestinal Inflammation ◽  
Mucosal Injury ◽  
Interferon Γ ◽  
Dual Function ◽  
Intestinal Epithelial ◽  
Inhibition Of Proliferation ◽  
Protein Levels ◽  
Sustained Inhibition ◽  
Sustained Activation

The proinflammatory cytokine interferon γ (IFNγ ) influences intestinal epithelial cell (IEC) homeostasis in a biphasic manner by acutely stimulating proliferation that is followed by sustained inhibition of proliferation despite continued mucosal injury. β-Catenin activation has been classically associated with increased IEC proliferation. However, we observed that IFNγ inhibits IEC proliferation despite sustained activation of Akt/β-catenin signaling. Here we show that inhibition of Akt/β-catenin–mediated cell proliferation by IFNγ is associated with the formation of a protein complex containing phosphorylated β-catenin 552 (pβ-cat552) and 14.3.3ζ. Akt1 served as a bimodal switch that promotes or inhibits β-catenin transactivation in response to IFNγ stimulation. IFNγ initially promotes β-catenin transactivation through Akt-dependent C-terminal phosphorylation of β-catenin to promote its association with 14.3.3ζ. Augmented β-catenin transactivation leads to increased Akt1 protein levels, and active Akt1 accumulates in the nucleus, where it phosphorylates 14.3.3ζ to translocate 14.3.3ζ/β-catenin from the nucleus, thereby inhibiting β-catenin transactivation and IEC proliferation. These results outline a dual function of Akt1 that suppresses IEC proliferation during intestinal inflammation.

Long-time strength of isotropic polymers in plane stress state

1969 ◽  
Vol 2 (6) ◽  
pp. 529-535
Author(s):  
O. Ya. Korf ◽  
A. M. Skudra
Keyword(s):  
Stress State ◽  
Plane Stress ◽  
Plane Stress State ◽  
Long Time ◽  
Time Strength

scholarly journals A novel hydrocortisone-ethanol gel ointment for treating atopic dermatitis (eczema) in children: A double blind, randomized, controlled clinical trial

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Dale Lawrence Pearlman
Keyword(s):  
Clinical Trial ◽  
Atopic Dermatitis ◽  
Controlled Study ◽  
Controlled Clinical Trial ◽  
Severe Atopic Dermatitis ◽  
Double Blind ◽  
Parental Concerns ◽  
Score Improvement ◽  
Long Time ◽  
Secondary Endpoints

Importance: Current treatments for moderate to severe atopic dermatitis (AD) in children are limited by incomplete efficacy, long time to benefit, and parental concerns about safety. This study evaluated a novel ointment for treating AD containing 0.83% hydrocortisone and 17% dispersed ethanol gel micro bubbles.  Observations:20 children with moderate to severe AD participated in a one-week double blind, randomized, and controlled clinical trial. They were randomly assigned to apply BID either an ointment with 1% hydrocortisone ointment (HC) or a novel ointment containing 1% hydrocortisone and dispersed ethanol gel droplets (HC-EG).   The primary endpoint was superiority of HC-EG over HC ointment in SCORAD score improvement during therapy. A secondary endpoint was improvement in pruritus score during therapy. Both the primary and secondary endpoints were reached in this study. SCORAD score improved 74% on average with HC-EG ointment vs 41% with HC ointment (p=.02). Pruritus score improved 68% on average with HC-EG ointment vs 37% with HC ointment (p=.009). No toxicity requiring stopping therapy was observed in either treatment group.  Conclusions and Relevance: Inthis small controlled study HC-EG ointment was superior to HC ointment both in improving visible rash and pruritus of AD. Parents felt HC-EG ointment was safe because it contains no prescription corticosteroids, prescription immunosuppressants, or antibiotics. Independent larger studies would be a next step in evaluating further this new way to treat AD. 

scholarly journals Qualitative signs of digital dermatoglyphics as markers of diseases of atopic nature

2019 ◽  
pp. 5-12
Author(s):  
V. G. Cherkasov ◽  
О. Ye. Маievskyi ◽  
I. V. Serheta ◽  
I. M. Makarchuk ◽  
N. M. Smolko
Keyword(s):  
Allergic Rhinitis ◽  
Atopic Dermatitis ◽  
Bronchial Asthma ◽  
Young Women ◽  
Young Men ◽  
Statistical Processing ◽  
Random Pattern ◽  
Healthy Population ◽  
Atopic Diseases ◽  
Polygenic Inheritance

The lawfulness of the application of the method of dermatoglyphics in the study of atopic diseases is ensured by the polygenic inheritance of signs of dermatoglyphics, on the one hand, and the pathogenetic heterogeneity of these diseases, on the other hand, as well as high informative ability of signs of dermatoglyphics as markers of diseases of hereditary and multifactorial nature. The purpose of the study is to detect differences in qualitative signs of digital dermatoglyphics between patients with atopic dermatitis, allergic rhinitis and bronchial asthma. Primary indicators of digital dermatoglyphics of sick young men and young women of the Podillia region are taken from the data bank of the research center of the National Pirogov Memorial Medical University, Vinnytsya and were used in previous studies when compared with the practically healthy population of this region. Imprints were obtained by the method of “printing ink” by Gladkova T. D. By the method of Cummins H. and Midlo Ch. a dermatological study was performed for 320 young men and young women with allergic rhinitis (n=69), bronchial asthma (n=108) and atopic dermatitis (n=143). The frequency and location of 8 types of finger patterns were subject to analysis. Statistical processing of the obtained results was carried out in the package “Statistica 6.1” using nonparametric methods. The reliability of the difference in values between independent qualitative values was determined by the formula of Weber E. (1961). The specificity of the digital typology of atopic diseases is established, which is based on the differences in the frequency and location of the whorl, central pocket and arches between the young men, except those indicated - a random pattern between young women, patients with atopic dermatitis, bronchial asthma, allergic rhinitis. Additionally, when comparing young men, patients with allergic rhinitis with patients with bronchial asthma and atopic dermatitis - ulnar loop; for bronchial asthma with patients with allergic rhinitis and atopic dermatitis - lateral pocket loop (in young men) and ulnar, lateral pocket and double loops (in young women); when comparing young men, patients with atopic dermatitis with patients with bronchial asthma, and allergic rhinitis - a random pattern.

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