scholarly journals Microbiological Quality of Seawater and Mussels (Mytilus galloprovincialis, Lamarck 1819) in the Dubrovnik Coastal Area (Southeastern Adriatic)

Naše more ◽  
2021 ◽  
Vol 68 (3) ◽  
pp. 137-149
Author(s):  
Svjetlana Bobanović-Ćolić ◽  
◽  
Jakša Bolotin ◽  
Nikša Glavić ◽  
Enis Hrustić
Keyword(s):  
Coastal Water ◽  
Coastal Area ◽  
Mytilus Galloprovincialis ◽  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Faecal Coliform ◽  
Coliform Bacteria ◽  
Anthropogenic Factors ◽  
Mussel Tissue ◽  
Surrounding Water

This research was conducted between March 2016 and March 2017 as the first study on the water and mussel quality in the coastal area of Dubrovnik (Croatia). Bacteriological analyses were conducted simultaneously on wild mussels (Mytilus galloprovincialis) tissue and their ambient water. The total numbers of heterotrophic bacteria, total coliforms, faecal coliforms and faecal streptococci in the water were determined. Concentrations of Escherichia coli were measured in mussel. The investigated stations are located in coastal water, which is particularly influenced by climatic and anthropogenic factors interacting at the boundaries between the land and freshwater and seawater environments. Seasonal variables, such as rainfall and riverine network characteristics, determine the delivery of bacteria (including E.coli) to coastal water. Direct methods of assessing the density of heterotrophic bacteria at all sampling stations showed the characteristics of an oligotrophic area. The abundance of faecal coliform bacteria was in all cases higher within mussel tissue than in the surrounding water where levels were sometimes undetectable. Maximum E. coli concentrations in mussels and seawater were regularly recorded through the summer, with the exceptions of April 2016 and January 2017 at two stations following precipitation. Minimum faecal coliform concentrations were recorded in the winter.

scholarly journals The health-related microbiological quality of bottled drinking water sold in Dar es Salaam, Tanzania

2006 ◽  
Vol 5 (1) ◽  
pp. 179-185 ◽  
Author(s):  
Gabriel R. Kassenga
Keyword(s):  
Drinking Water ◽  
Heterotrophic Bacteria ◽  
Tap Water ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Dar Es Salaam ◽  
Bottled Water ◽  
Faecal Coliform ◽  
Coliform Bacteria

The consumption of bottled and plastic-bagged drinking water in Tanzania has increased largely because of the deteriorating quality of tap water. It is uncertain whether these water products are safe for drinking. In this study, the microbiological quality of bottled and plastic-bagged drinking water sold in Dar es Salaam, Tanzania, was investigated. One hundred and thirty samples representing 13 brands of bottled water collected from shops, supermarkets and street vendors were analysed for total coliform and faecal coliform organisms as well as heterotrophic bacteria. These were compared with 61 samples of tap water. Heterotrophic bacteria were detected in 92% of the bottled water samples analysed. Total and faecal coliform bacteria were present in 4.6% and 3.6%, respectively, of samples analysed with a tendency for higher contamination rates in plastic-bagged drinking water. Microbiological quality of tap water was found to be worse compared with bottled water, with 49.2% and 26.2% of sampling points showing the presence of total coliform and faecal coliform organisms, respectively. The results suggest caution and vigilance to avert outbreaks of waterborne diseases from these types of drinking water.

A survey of the microbial quality of wastewaters in Ismailia, Egypt and the implications for wastewater reuse

1997 ◽  
Vol 35 (11-12) ◽  
pp. 211-217 ◽  
Author(s):  
R. Stott ◽  
T. Jenkins ◽  
M. Shabana ◽  
E. May
Keyword(s):  
Constructed Wetlands ◽  
Sewage Treatment ◽  
Microbiological Quality ◽  
Faecal Coliform ◽  
Treated Wastewater ◽  
Coliform Bacteria ◽  
Intestinal Helminths ◽  
Practical Applications ◽  
Helminth Eggs ◽  
Raw Wastewater

The occurrence of parasite ova, cysts and faecal coliforms in urban wastewater and their removal was determined after sewage treatment in gravel bed hydroponic (GBH) constructed wetlands receiving conventionally treated wastewater at Abu Attwa, Ismailia. Samples of raw wastewater and GBH influent and effluents were examined for eggs of intestinal helminths over two months in 1995. All raw wastewater samples were found to be positive for Ascaris lumbricoides but eggs of Hymenolepis spp, Trichuris spp, Ancylostoma duodenale and Toxocara spp were also detected in raw and conventionally treated wastewaters. In Ismailia, the concentration of eggs of human intestinal helminths in raw wastewater ranged from 6–42/L. In the influent to the GBH beds, the concentration of helminth eggs was reduced and ranged from 0–11/L; eggs of Ascaris were found in 28% of the samples. No helminth eggs were recovered from GBH treated effluents. Cysts of protozoa and faecal coliform bacteria were also removed by GBH beds to some degree. Raw wastewater contained cysts of the protozoa, Entamoeba coli, E. histolytica and Giardia spp. Although the density and diversity of species was reduced after treatment in the GBH beds, some amoebic cysts were found in the effluents. Faecal coliform removal averaged 2–3 logs during sewage treatment in GBH beds but effluents did not satisfy WHO guidelines for unrestricted irrigation. This survey indicates that GBH beds have the capacity to remove pathogens from wastewaters. Improvement in wastewater quality after GBH treatment satisfied WHO microbiological quality guidelines for restricted irrigation. With a retention time of 6h, GBH constructed wetlands have practical applications for wastewater treatment for safe reuse in Egypt.

scholarly journals Bacteriological and Parasitological Assessment of Water use in Selected Abattoirs in Port Harcourt Metropolis, Rivers State, Nigeria

2021 ◽  
pp. 19-25
Author(s):  
Eze Chinwe Nwadiuto ◽  
Ihua Nnenna ◽  
Ijewere Blessing Ofureb
Keyword(s):  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Cross Sectional Study ◽  
Coliform Bacteria ◽  
Isolation And Identification ◽  
Surrounding Water ◽  
Cross Sectional ◽  
Port Harcourt ◽  
Microbiological Techniques ◽  
Rivers State

In the abattoir, large amount of water is being used in processing operations; and this produces huge amount of wastewater which empty into the surrounding water bodies. The cross-sectional study followed the conventional microbiological techniques of macroscopy, microscopy, isolation, and identification to assess the bacteriological and parasitological examination of water sources in selected abattoirs in Port Harcourt Metropolis, Rivers State, Nigeria. The result of microbial population showed Total Coliform ranging from 5 x 101cfu/ml – 2.80 x 102cfu/ml and Faecal Coliform bacteria of 2.80 x 101 cfu/ml -1.85 x 102 cfu/ml. Total Heterotrophic bacteria ranged from 7.8 x101cfu/ml to 4.2 x102cfu/ml. The mean microbial counts; 4.86 x101 cfu/ml Fecal Coliform, 9.65 x 101cfu/ml Total Coliform and 1.82 x 102cfu/ml Heterotrophic Count. The isolates identified were Klebsiella spp, Shigella spp., Salmonella typhymurium, E. coli, Proteus spp., Salmonella paratyphi, Vibrio cholera. Nkpor village abattoir recorded highest occurrence of bacteria isolate with a total of 50(22.03%) followed by Iwofe 48(21.15%). Water sample from ponds had a higher parasitic contamination while samples from the borehole were free from parasitic infestation. Water samples for parasitological assessment were filtered and examined for parasitic organism using standard methods. Four different parasites were identified in the study, they include Ascaris lumbricoides (32.5%), Entamoeba histolytica (22.5%), Giardia lamblia (17.5%) and the least Taenia spp (7.5%). Nkpor-village abattoir had the highest level of contamination with (30.0%) parasite occurrence, followed by Mgboshimini abattoir (15.0%). The use of unsafe water for meat processing by butchers, portends a serious public health risk to consumers who purchase the meat, from these abattoirs. Therefore, government should endeavour to make borehole water available to all the abattoirs in Port Harcourt.

Prevalence of Listeria monocytogenes in, and Microbiological and Sensory Quality of, Rainbow Trout, White fish, and Vendace Roes from Finnish Retail Markets

2003 ◽  
Vol 66 (10) ◽  
pp. 1832-1839 ◽  
Author(s):  
HANNA MIETTINEN ◽  
ANNE ARVOLA ◽  
TIINA LUOMA ◽  
GUN WIRTANEN
Keyword(s):  
Rainbow Trout ◽  
Listeria Monocytogenes ◽  
Sensory Quality ◽  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Coregonus Lavaretus ◽  
Retail Markets ◽  
Sensory Qualities

The prevalence of Listeria monocytogenes in retail roe, as well as the microbiological and sensory qualities of the roe, were studied for three fish species under three different storage conditions. A total of 147 Finnish rainbow trout (Oncorhynchus mykiss), white fish (Coregonus lavaretus), vendace (Coregonus albula), and burbot (Lota lota) roe samples were bought fresh, frozen, or frozen-thawed from Finnish retail markets. The overall prevalence of L. monocytogenes was 5%; however, the prevalence of the pathogen in fresh roe was 18%. Fresh-bought roe tested positive for Listeria spp. and for L. monocytogenes, respectively, 5 and 20 times as often as did frozen and frozen-thawed roe products combined. The microbiological quality (analyzed as total aerobic heterotrophic bacteria and coliform bacteria) of 78% of the roe samples was unacceptable. Frozen roe samples were found to have the best microbiological quality. According to the results of a sensory evaluation, at least one sensory attribute (appearance, odor freshness, texture, and freshness of taste) was unacceptable for 29% of the roe samples studied. The sensory quality of roe samples bought fresh was better than that of roe samples bought frozen or frozen-thawed. From the results of this study, it is concluded that both the microbiological and the sensory qualities of roe at the retail level need to be improved.

scholarly journals Microbiological quality of frozen black mussels (Mytilus galloprovincialis, Lamarck, 1819) purchased from markets in the İzmir Province of Turkey

2021 ◽  
Vol 38 (2) ◽  
pp. 167-172
Author(s):  
Bülent Kafa ◽  
Berna Kılınç
Keyword(s):  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Mytilus Galloprovincialis ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Human Consumption ◽  
Salmonella Spp ◽  
Consumer Safety ◽  
Vibrio Spp

The purpose of this study was to examine the microbiological quality and consumer safety of frozen black mussels purchased from four different markets (A, B, C, D) in the İzmir Province of Turkey. A total of 36 frozen black mussel packages, which contained approximately 50 mussels in each, were purchased from the markets. They were not only examined for total mesophilic, psychrotrophic, coliform and fecal coliform bacteria count but also investigated for pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Vibrio spp. According to the results of the mesophilic bacteria counts, frozen black mussels did not exceed the microbiological limits set for safe consumption. The findings showed that frozen black mussels purchased from (A, B, C, D) of markets were safe for human consumption because pathogenic bacteria species such as E.coli, Salmonella spp., S. aureus, L. monocytogenes and Vibrio spp. were not present in the frozen mussel samples.

Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Wanda Aulya ◽  
Fadhliani Fadhliani ◽  
Vivi Mardina
Keyword(s):  
Drinking Water ◽  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inspection Method ◽  
Sample Number ◽  
Probable Number ◽  
Fecal Coliform Bacteria

Water is the main source for life and also the most severe substance caused by pollution. The mandatory parameters for determining microbiological quality of drinking water are total non-fecal Coliform bacteria and Coliform fecal (Escherichia coli). Coliform bacteria are a group of microorganisms commonly used as indicators, where these bacteria can be a signal to determine whether a water source has been contaminated by bacteria or not, while fecal Coliform bacteria are indicator bacteria polluting pathogenic bacteria originating from human feces and warm-blooded animals (mammals) . The water inspection method in this study uses the MPN (Most Probable Number) method which consists of 3 tests, namely, the presumption test, the affirmation test, and the reinforcement test. The results showed that of 15 drinking water samples 8 samples were tested positive for Coliform bacteria with the highest total bacterial value of sample number 1, 15 (210/100 ml), while 7 other samples were negative. From 8 positive Coliform samples only 1 sample was stated to be negative fecal Coliform bacteria and 7 other samples were positive for Coliform fecal bacteria with the highest total bacterial value of sample number 1 (210/100 ml).

MICROBES PARAMETERS OF WATER QUALITY FOR AQUACULTURE ON PARI ISLAND WATERS

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Pathogenic Bacteria ◽  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Total Cell ◽  
Epifluorescence Microscopy ◽  
Coliform Bacteria ◽  
Total Density ◽  
Shellfish Aquaculture ◽  
Microbiological Parameters ◽  
Phosphate Nitrate

The objective of this research was to evaluate waters quality in Pari island waters for aquaculture purpose based on bacteriological information conducted in Mei and September 2011. Microbiological parameters analyzed were total density of bacteria for coliforms, E.coli, pathogenic, heterotrophic, halotoleran, phosphate-nitrate-ammonia breaker, and total cells. Method to analyze coliform bacteria was filtration, identification of pathogenic bacteria using biochemical test, density analises for heterotrophic bacteria, analises for phosphate-nitrate-ammonia breaker bacteria using pour plate, and total cell using Acridine Orange Epifluorescence Microscopy. Results showed that the abundance of total coliform cell was about 1000-7000 colony forming unit (cfu)/100 ml. The abundance of heterotrophic, halotolerant, phosphate-nitrate-ammonia bacteria in seawater was (3.6-4.3)x105 cfu/ml, (1.1-1.3)x105 cfu/ml, (0.5-3.44)x103cfu/ml; and (3.6-6.7)x105 cfu/ml, (1.6-2.7)x105 cfu/ml, (0.6-5.22)x103 cfu/ml in sediment, respectively. The total cell of bacteria was (0.05-2.1)x107cells/ml. The dog-conch (Strombus turturella) and blood-clamps (Anadara granosa) can survive in Pari Island and there was a significant increase in sea grass litter with growth average of 0.67 mm/day and 0.90 mm/day. During snails and clamps growth, there were found several genus of pathogenic bacteria such as Salmonella, Vibrio, Aeromonas, Pseudomonas, Citrobacter, Proteus, Shigella, Hafnia, and Yersinia. The results showed that Pari island waters was suitable for developing shellfish aquaculture dog conch and blood clamps. Keywords: bacteria, parameter, shellfish, aquaculture.

scholarly journals Microbial Load on Paper/Polymer Currency and Coins

1970 ◽  
Vol 9 ◽  
pp. 105-109 ◽  
Author(s):  
Tista Prasai ◽  
Kayo Devi Yami ◽  
Dev Raj Joshi
Keyword(s):  
Key Words ◽  
Heterotrophic Bacteria ◽  
Science And Technology ◽  
Microbial Load ◽  
Disease Spread ◽  
Coliform Bacteria ◽  
Aerobic Bacteria ◽  
Contamination Level

Currency notes and coins serve as an agency of transmission of microorganisms since they are passed freely from hand to hand as a medium of exchange. A research, with an objective to explore the microbial load on Nepalese paper/ polymer currency notes and coins, was carried out at the Environment Laboratory of Nepal Academy of Science and Technology, Khumaltar from November 2006 to May 2007. All together 63 samples of paper/polymer notes and coins from different professionals of different places at Kathmandu were collected and analyzed for the presence of microorganisms. Among the total tested paper/ polymer and coin samples, 98.4% were found to have heterotrophic aerobic bacteria, 87.3% were contaminated with coliform bacteria and 79.4% showed presence of Staphylococci. Contamination level was found in increasing order of coins> polymer notes>paper notes. The presence of high microbial load on currency notes and coins indicate the potentials of such currencies for possible disease spread in the human communities. Key words: currency notes; coins; heterotrophic bacteria; Coliform bacteria; Staphylococci DOI: 10.3126/njst.v9i0.3173 Nepal Journal of Science and Technology 9 (2008) 105-109

Fungi as environmental microbiological indicators

1998 ◽  
Vol 38 (12) ◽  
pp. 155-162 ◽  
Author(s):  
B. Mendes ◽  
P. Urbano ◽  
C. Alves ◽  
J. Morais ◽  
N. Lapa ◽  
...  
Keyword(s):  
Coastal Area ◽  
Microbiological Quality ◽  
Indicator Organisms ◽  
Saprophytic Fungi ◽  
Keratinolytic Fungi ◽  
Quality Objective ◽  
Microbiological Indicators ◽  
Satisfactory Quality

An evaluation of the mycological quality of the sand beaches of the Lisboa and Vale do Tejo coastal area in Portugal was undertaken in May-October 1994. The keratinolytic fungi, yeasts, potential pathogenic and allergic and/or environmental saprophytic fungi were analysed. The results for the yeast Candida were evaluated according to the “Proposed Guidelines for the Microbiological Quality of Sand”. The data showed good/satisfactory quality of the sand beaches for the genus Candida. The results indicate that the allergic and/or environmental saprophytic fungi were the most common in sand beaches. The fungi Scopulariopsis and the yeast Candida could be used as specific indicator organisms of sand beaches quality. A new quality objective is introduced that will contribute to improve the sand beaches quality.

scholarly journals Sustainable Management of Mediterranean Coastal Fresh and Transitional Water Bodies: a Socio-Economic and Environmental Analysis of Changes and Trends to Enhance and Sustain Stakeholders Benefits

2010 ◽  
Keyword(s):  
Water Management ◽  
European Commission ◽  
Case Studies ◽  
Coastal Water ◽  
Environmental Analysis ◽  
Coastal Area ◽  
Sustainable Management ◽  
Water Bodies ◽  
International Conference ◽  
Mediterranean Countries

This volume presents the Proceedings of the International Conference of the WADI project held in Malta, 5-8 November 2008, at the end of the project itself. The WADI project funded by the European Commission, was carried out from 2006 to 2008 by a consortium of researchers from European and Mediterranean countries, and was focused on coastal water bodies, aiming at integrating water management and the needs of all stakeholders. The Proceedings illustrate some of the outcomes of the WADI project that focused on case studies represented by water bodies in the Mediterranean coastal area.

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