scholarly journals Metabolomic Analysis of Rhizosphere Soil Fertility in Maize (Zea mays) at Milking Stage

2021 ◽  
Vol 25 (03) ◽  
pp. 555-566
Author(s):  
Yunpeng Luan
Keyword(s):  
Total Nitrogen ◽  
Rhizosphere Soil ◽  
Potassium Dichromate ◽  
Colorimetric Method ◽  
Acid Soils ◽  
Ammonium Nitrogen ◽  
Available Phosphorus ◽  
Kjeldahl Method ◽  
Phosphorus And Potassium ◽  
Tof Ms

In this study rhizosphere soil of cornat a milk stage was collected to investigate characteristic metabolites and their potential functions. Total nitrogen, organic matters, ammonium nitrogen, pH values, available phosphorus and potassium were determined by semimicro-Kjeldahl method, potassium dichromate (external heating) method, indophenol blue colorimetric method, potentiometry, NaHCO3leaching-molybdenum-antimony colorimetric method and NH4OAc leaching-flame spectrometry, respectively. In addition, UPLC-Q/TOF-MS was adopted for non-targeted metabolomicanalysis. As revealed by results, the total nitrogen contents in soils collected from Dongchuang (i.e., DCMRS for short)0.67±0.14mg/kg lower than from Fumin (i.e., FMMRS for short); moreover, both DCMRS and FMMRS were acid soils. DCMRS contains higher levels of AN (Ammonium nitrogen), SOC (Soil organic carbon), and AP (Available phosphorus) than FMMRS. The amount of TN (Total nitrogen) contained in FMMRS soil was2.410±0.422mg/kg, which is higher than DCMRS. All data derived from UPLC-Q/TOF-MS met the corresponding requirements for further analysis. Metabolites such as 2-methyl-1-propylamine, gamma-butyrolactone and 3-methyl-1-butylamine were detected in DCMRS and FMMRS samples. Several pathways were included, such as lipid metabolism, xenobioticsbiodegradation and metabolism, terpenoids and polyketides, and amino acid metabolism. Through comparison of FMMRS and DCMRS, metabolic pathways associated with nitrogen, carbon, and antibiotic metabolism including iron transport were significantly different between them. Taken together, FMMRS is more fertile, less acidic, and higher in nitrogen than DCMRS.© 2021 Friends Science Publishers

scholarly journals Biological function of Klebsiella variicola and its effect on the rhizosphere soil of maize seedlings

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9894
Author(s):  
Lijuan Yang ◽  
Kejun Yang
Keyword(s):  
Biological Function ◽  
Rhizosphere Soil ◽  
Potassium Dichromate ◽  
Colorimetric Method ◽  
Soil Salinization ◽  
Available Phosphorus ◽  
Plant Growth Promoting Bacteria ◽  
Alkali Stress ◽  
Maize Seedlings ◽  
Plant Growth Promoting

Background Deterioration of the ecological environment in recent years has led to increasing soil salinization, which severely affects the cultivation of agricultural crops. While research has focused on improving soil environment through the application of pollution-free microbial fertilizers, there are relatively few plant growth-promoting bacteria suitable for saline-alkali environments. Although Klebsiella variicola can adapt to saline-alkali environments to successfully colonize rhizosphere microenvironments, only a few studies have investigated its role in promoting crop growth. Its effect on the crop rhizosphere soil microenvironment is especially unclear. Methods In this study, the biological function of K. variicola and its colonization in maize seedling rhizosphere soil were studied in detail through selective media and ultraviolet spectrophotometry. The effects of K. variicola on the rhizosphere soil microenvironment and the growth of maize seedlings in saline-alkali and neutral soils were systematically analysed using the colorimetric method, the potassium dichromate volumetric method, and the diffusion absorption method. Results Our results showed that K. variicola played a role in indole acetic acid, acetoin, ammonia, phosphorus, and potassium production, as well as in nitrogen fixation. A high level of colonization was observed in the rhizosphere soil of maize seedlings. Following the application of K. variicola in neutral and saline-alkali soils, the nutrient composition of rhizosphere soil of maize seedlings increased in varying degrees, more notably in saline-alkali soil. The content of organic matter, alkali-hydrolysable nitrogen, available phosphorus, available potassium, alkaline phosphatase, sucrase, urease, and catalase increased by 64.22%, 117.39%, 175.64%, 28.63%, 146.08%, 76.77%, 86.60%, and 45.29%, respectively, insaline-alkalisoil. Conclusion K.variicola, therefore, performed a variety of biological functions to promote the growth of maize seedlings and effectively improve the level of soil nutrients and enzymes in the rhizosphere of maize seedlings, undersaline-alkali stress conditions. It played an important role in enhancing the rhizosphere microenvironment of maize seedlings under saline-alkali stress.

scholarly journals Status of Soil Fertility in a Community Forest of Nepal

2013 ◽  
Vol 1 (1) ◽  
pp. 56-67 ◽  
Author(s):  
Anup KC ◽  
Govinda Bhandari ◽  
Subigya Prabhat Wagle ◽  
Yubraj Banjade
Keyword(s):  
Organic Matter ◽  
Forest Soil ◽  
Total Nitrogen ◽  
Complex Mixture ◽  
Soil Parameters ◽  
Available Phosphorus ◽  
Community Forest ◽  
The Status ◽  
Phosphorus And Potassium ◽  
Living Organisms

Soil is a complex mixture of mineral nutrients, organic matter, water, air and living organisms. The primary nutrients for plant growth are organic matter, nitrogen, phosphorus and potassium. In order to find the status of pH, organic matter (C), total nitrogen (N), available phosphorus (P) and available potassium (K) in forest soil, the study was conducted in Ghwangkhola Sapaude Babiyabhir Community Forest in Putali Bazaar Municipality-8, Syangja, Nepal. Soil parameters are analyzed through different standard methods followed worldwide by many soil scientists. Soil pH of sample in all three strata was slightly acidic and varies from 5.7 to 7.18. The carbon percentage was high and varies from 0.65% to 2.39%. The total nitrogen in soil was medium and varies from 0.09% to 0.12%. The concentration of available phosphorus in soil was high and varies from 73.71 kg/ha-93.23 kg/ha. The concentration of available potassium on soil was quiet low and varies from 2.54 kg/ha-4.23 kg/ha. Higher organic matter in the forest land indicate low activities of nitrogen losing process, which is due to the closed nutrient cycling and minimal disturbance in the natural forest system. So, addition of fertilizer rich in potassium and increasing pH is recommended to maintain potassium fertility and neutral pH in the forest soil. DOI: http://dx.doi.org/10.3126/ije.v1i1.8528 International Journal of Environment Vol.1(1) 2013: 56-67

scholarly journals Influence of Slope Gradient and Aspect on Soil Organic Carbon Content in the Region of Niš, Serbia

2021 ◽  
Vol 13 (15) ◽  
pp. 8332
Author(s):  
Snežana Jakšić ◽  
Jordana Ninkov ◽  
Stanko Milić ◽  
Jovica Vasin ◽  
Milorad Živanov ◽  
...  
Keyword(s):  
Land Use ◽  
Spatial Distribution ◽  
Organic Carbon ◽  
Soil Organic Carbon ◽  
Slope Aspect ◽  
Available Phosphorus ◽  
Organic Carbon Content ◽  
Slope Gradient ◽  
Phosphorus And Potassium ◽  
Uncultivated Land

Topography-induced microclimate differences determine the local spatial variation of soil characteristics as topographic factors may play the most essential role in changing the climatic pattern. The aim of this study was to investigate the spatial distribution of soil organic carbon (SOC) with respect to the slope gradient and aspect, and to quantify their influence on SOC within different land use/cover classes. The study area is the Region of Niš in Serbia, which is characterized by complex topography with large variability in the spatial distribution of SOC. Soil samples at 0–30 cm and 30–60 cm were collected from different slope gradients and aspects in each of the three land use/cover classes. The results showed that the slope aspect significantly influenced the spatial distribution of SOC in the forest and vineyard soils, where N- and NW-facing soils had the highest level of organic carbon in the topsoil. There were no similar patterns in the uncultivated land. No significant differences were found in the subsoil. Organic carbon content was higher in the topsoil, regardless of the slope of the terrain. The mean SOC content in forest land decreased with increasing slope, but the difference was not statistically significant. In vineyards and uncultivated land, the SOC content was not predominantly determined by the slope gradient. No significant variations across slope gradients were found for all observed soil properties, except for available phosphorus and potassium. A positive correlation was observed between SOC and total nitrogen, clay, silt, and available phosphorus and potassium, while a negative correlation with coarse sand was detected. The slope aspect in relation to different land use/cover classes could provide an important reference for land management strategies in light of sustainable development.

scholarly journals Tillage in Relation to Distribution of Nutrients and Organic Carbon in the Soil

2011 ◽  
Vol 57 (1) ◽  
pp. 21-30
Author(s):  
Božena Šoltysová ◽  
Martin Danilovič
Keyword(s):  
Organic Carbon ◽  
Soil Organic Carbon ◽  
Total Nitrogen ◽  
Spring Barley ◽  
Conventional Tillage ◽  
Soil Tillage ◽  
Reduced Tillage ◽  
Available Phosphorus ◽  
No Tillage ◽  
The Difference

Tillage in Relation to Distribution of Nutrients and Organic Carbon in the SoilChanges of total nitrogen, available phosphorus, available potassium and soil organic carbon were observed on gleyic Fluvisols (locality Milhostov) at the following crops: grain maize (2005), spring barley (2006), winter wheat (2007), soya (2008), grain maize (2009). The experiment was realized at three soil tillage technologies: conventional tillage, reduced tillage and no-tillage. Soil samples were collected from three depths (0-0.15 m; 0.15-0.30 m; 0.30-0.45 m). The ratio of soil organic carbon to total nitrogen was also calculated.Soil tillage affects significantly the content of total nitrogen in soil. The difference between the convetional tillage and soil protective tillages was significant. The balance showed that the content of total nitrogen decreased at reduced tillage by 5.2 rel.%, at no-tillage by 5.1 rel.% and at conventional tillage by 0.7 rel.%.Similarly, the content of organic matter in the soil was significantly affected by soil tillage. The content of soil organic carbon found at the end of the research period was lower by 4.1 rel.% at reduced tillage, by 4.8 rel.% at no-tillage and by 4.9 rel.% at conventional tillage compared with initial stage. The difference between the convetional tillage and soil protective tillages was significant.Less significant relationship was found between the soil tillage and the content of available phosphorus. The balance showed that the content of available phosphorus was increased at reduced tillage (by 4.1 rel.%) and was decreased at no-tillage (by 9.5 rel.%) and at conventional tillage (by 3.3 rel.%).Tillage did not significantly affect the content of available potassium in the soil.

scholarly journals Improvement of Tea (Camellia sinensis L.) Soil Properties by Growing Different Green Crops

2015 ◽  
Vol 12 (2) ◽  
pp. 34-38 ◽  
Author(s):  
Ashim Kumar Saha ◽  
Apu Biswas ◽  
Abdul Qayyum Khan ◽  
Md. Mohashin Farazi ◽  
Md. Habibur Rahman
Keyword(s):  
Organic Carbon ◽  
Soil Properties ◽  
Total Nitrogen ◽  
Soil Ph ◽  
Nutritional Value ◽  
Soil Health ◽  
Critical Value ◽  
Available Phosphorus ◽  
Organic Carbon Content

Long-term tea cultivation has led to degradation of the soil. Old tea soils require rehabilitation for restoring soil health. Soil rehabilitation by growing different green crops can break the chain of monoculture of tea. An experiment was conducted at The Bangladesh Tea Research Institute (BTRI) Farm during 2008-2011 to find out the efficiency of different green crops on the improvement of soil properties. Four green crops such as Guatemala, Citronella, Mimosa and Calopogonium were grown to develop the nutritional value of the degraded tea soil. Soil samples were collected and analyzed before and at the end of experiment. Soil pH was increased in all four green crops treated plots with the highest increase in Citronella treated plots (from 4.1 to 4.5). Highest content of organic carbon (1.19%) and total nitrogen (0.119%) were found in Mimosa and Calopogonium treated plots, respectively. Concentration of available phosphorus, calcium and magnesium in all green crops treated plots were above the critical values, while available potassium content was above the critical value in Guatemala, Citronella and Mimosa treated plots. Changes in soil pH and available potassium were significant, while changes in organic carbon content, total nitrogen and available calcium were insignificant. Changes in available phosphorus and magnesium were significant. The Agriculturists 2014; 12(2) 34-38

scholarly journals Adsorption of Copper in Soil and its Dependence on Physical and Chemical Properties

2018 ◽  
Vol 66 (1) ◽  
pp. 219-224 ◽  
Author(s):  
Vítězslav Vlček ◽  
Miroslav Pohanka
Keyword(s):  
Soil Properties ◽  
Solid Phase ◽  
Chemical Properties ◽  
Total Content ◽  
Exchange Capacity ◽  
Soil Reaction ◽  
Available Phosphorus ◽  
Copper Adsorption ◽  
Phosphorus And Potassium ◽  
Physical And Chemical

Soil samples (n = 11) were collected in the chernozem areas of the Czech Republic (the Central Europe) from the topsoil and used as representative samples. All sampling areas have been used for agricultural purposes (arable soil) and they were selected as typical representatives of agricultural soil. These samples represented the soil with same genesis (to reduction differencies between soil types) but with different soil properties (physical and chemical). Complete chemical and physical analyses were made for confirmation of copper adsorption on solid phase: we analysed the particle size distribution, content of oxidizable carbon (Cox), the cation exchange capacity (CEC), supply of exchange calcium, magnesium, sodium, phosphorus and potassium, soil reaction and the total supply of Fe, Al, Mn, Ca, Mg, K, P and N. The strongest simple correlation between analysed soil properties and copper concentration had content of available magnesium (r = 0.44) and available phosphorus (r = −0.51). In the case of multiple correlations (i. e. collective influence of multiple soil properties) had the strongest influence combination of clay, soil reaction, total content of phosphorus, available magnesium and available phosphorus. The main influence of phosphorus and magnesium is evident. We suppose that copper and phosphorus enter into specific complex. Influence of these five soil properties can explain 92.7 % (r = 0.927) changes in the content of copper changes in the experiment.

scholarly journals State of Heavy Metals Pollution of Flooded Agricultural Land in the North Part of Republic of Srpska

2017 ◽  
Vol 17 (1) ◽  
pp. 19
Author(s):  
Tihomir Predić ◽  
Petra Nikić Nauth ◽  
Bojana Radanović ◽  
Alen Predić
Keyword(s):  
Heavy Metals ◽  
Soil Contamination ◽  
Agricultural Land ◽  
Total Content ◽  
Soil Samples ◽  
Available Phosphorus ◽  
The North ◽  
North Part ◽  
Grid Points ◽  
Phosphorus And Potassium

This paper presents the results of the research of soil contamination in flooded area located at the confluence of Bosna and Sava rivers. The sampling was performed according to a network of points Etrs89 reference European Network based on grid points of 500 m x 500 m. The total number of soil samples was 31 and 13 samples of sediment-mud. In the soil samples and sediment-mud samples various parameters were analyzed: pH H2O and 1 M KCl, humus (method by Tjurin), and the readily available phosphorus and potassium (AL method). The interpretation of total content of heavy metals in the soil was conducted according to Dutch Soil Remediation Circular (2009). The samples were analyzed for the total content of heavy metals: Pb, Cd, Cr, Ni, Zn, Cu (aqua regia digestion, AAS) and the elevated concentrations of Ni. However, Ni content in mud was from 240.5 mg/kg to 294.5 mg/kg, in soil it was from 128.9 mg/kg to 452.1 mg/kg. All mud samples were alkaline, and 79% of soil samples were alkaline and of neutral reaction. The analysis indicated that the flood in May 2014 was not caused by soil contamination with heavy metals, which however contributed to the increase of the overall content of these elements in the soil.

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