scholarly journals Respon Superovulasi Sapi Persilangan Belgian Blue dengan Metode yang Berbeda

2021 ◽  
Vol 21 (2) ◽  
pp. 178-186
Author(s):  
Fahrudin Darlian ◽  
Sri Wahjuningsih ◽  
Anny Rosmayanti ◽  
Sepatria Jodiansyah ◽  
Ludi Ahmad Jalaludin ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Recovery Rate ◽  
Embryo Quality ◽  
Response Rate ◽  
Single Injection ◽  
Fertilization Rate ◽  
Alternative Methods ◽  
Injection Technique ◽  
Crossbred Cattle ◽  
Embryo Recovery

ABSTRACT. Superovulasi merupakan suatu metode pemanfaatan induk betina unggul untuk menghasilkan embrio sebanyak-banyaknya untuk kegiatan transfer embrio. Tujuan penelitian ini membandingkan 2 teknik superovulasi yang berbeda pada sapi persilangan Belgian Blue. Penelitian dilakukan di Balai Embrio Ternak Cipelang Bogor dengan menggunakan 24 ekor sapi persilangan Belgian Blue dengan umur 2-3 tahun, masing-masing perlakuan 12 ekor. Penelitian dilakukan secara eksperimen dengan dua perlakuan, P1 = Superovulasi menggunakan penyuntikan FSH selama 3 hari pada pagi dan sore (metode konvensional) dengan dosis 400 mg FSH dalam 20 ml pelarut dan P2 = Superovulasi menggunakan penyuntikan tunggal (satu kali) FSH dengan dosis 400 mg FSH dalam 3 ml pelarut . Parameter pada penelitian ini adalah Jumlah Corpus luteum (CL), respon rate, perolehan embrio, kualitas embrio, recovery rate, dan tingkat fertilisasi. Data yang diperoleh diuji dengan uji T tidak berpasangan. Hasil analisa data menunjukkan hasil yang tidak berbeda nyata (P0,05) pada jumlah CL P1 : 8,42+3,06 dan P2 : 6,08+4,74; Respon rate P1 : 100% dan P2 : 75%; Total perolehan embrio P1 : 6,08+2,64 embrio dan P2 : 4,45+4,01 embrio; Embrio recovery rate P1 : 70,37+9,18% dan P2 : 61,33+12,12%; Embrio Layak Transfer P1 : 3,83+2,92 embrio dan P2 : 2,73+2,28 embrio; dan berbeda nyata (P0,05) pada rataan fertilisasi P1 : 79,10% dan P2 : 95,26%. Kesimpulan penelitian ini adalah teknik superovulasi penyuntikan tunggal FSH secara subkutan memberikan efek superovulasi dan menghasilkan embrio dengan jumlah dan kualitas yang tidak berbeda nyata dengan teknik superovulasi konvensional. (Superovulation Responses of Belgian Blue Crossbreed Cattle Treated with Different Superovulation Methods) ABSTRAK. Superovulation is a technique for producing a large number of embryos for embryo transfer using a genetically superior female. The purpose of this research was to compare two alternative methods of superovulation in Belgian Blue crossbreed cattle. The study used 24 Belgian Blue crossbred cattle aged 2-3 years, including 12 cows per treatment, at the National Livestock Embryo Center of Cipelang in Bogor. The research was done in an experimental setting using two different treatments, P1 = Superovulation using twice daily FSH injections for three days at a dose of 400 mg FSH dissolved in 20 ml of saline, and P2 = Superovulation using FSH single injections at a dose of 400 mg FSH dissolved in 3 ml of saline. The parameters in this study were the number of corpus luteum (CL), response rate, total number of embryo/ova collection, embryo quality, recovery rate, and fertilization rate. The data obtained were tested by unpaired T test. The results of data analysis showed results that were not significantly different (P0.05) on the number of CL P1 : 8.42+3.06 and P2 : 6.08+4.74; Response rate P1 : 100% and P2 : 75%; Total number of embryos collection P1 : 6.08+2.64 embryos and P2 : 4.45+4.01 embryos; Embryo recovery rate P1 : 70.37+9.18% and P2 : 61.33+12.12%; Transferable Embryos P1 : 3.83+2.92 embryos and P2 : 2.73+2.28 embryos; and significantly different (P0.05) on fertilization rate P1 : 79.10% and P2 : 95.26%. The conclusion of this study show that the subcutaneous FSH single injection technique induces superovulation and produces embryos that are similar in number and quality to those produced by conventional superovulation techniques.

scholarly journals Factors Affecting Embryo Recovery Rate, Quality, and Diameter in Andalusian Donkey Jennies

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1967
Author(s):  
J. Dorado ◽  
M. Bottrel ◽  
I. Ortiz ◽  
M. Díaz-Jiménez ◽  
B. Pereira ◽  
...  
Keyword(s):  
Developmental Stage ◽  
Embryo Transfer ◽  
Recovery Rate ◽  
Embryo Quality ◽  
Donor Age ◽  
Successive Cycle ◽  
Assisted Reproductive Technique ◽  
Factors Affecting ◽  
Embryo Recovery ◽  
Starting Point

Embryo transfer and the vitrification of embryos could be used for the conservation and recovery of endangered donkey breeds. It is important to develop techniques that optimize recovery rates and the cryotolerance of donkey embryos. This study evaluates factors affecting the recovery rate, quality, and diameter of embryos obtained from donor jennies as a starting point for the use of vitrification and embryo transfer in the conservation of the Andalusian donkey. A total of 100 embryos were recovered out of 124 estrous cycles (80.6%). The donor jenny affected the rates of positive flushings (PFR; p = 0.040) and embryo recovery (ERR; p < 0.05) as well as embryo quality (p = 0.004). ERR was also affected by the number of flushings (p < 0.001), donor age (p < 0.05), successive cycle within donor (p < 0.001), and jacks (p < 0.05). Number of flushings (p < 0.001) and jack (p < 0.05) had a significant effect on PFR, whereas the day of flushing influenced the developmental stage (p < 0.001), embryo quality (p < 0.05), and diameter of embryos (p < 0.001). The number of flushings significantly influenced the diameter (p = 0.038) and embryo developmental stage (p = 0.001), whereas the developmental stage was statistically different between herds (p = 0.020). The factors influencing the success of this assisted reproductive technique were donor jenny, donor age, successive cycle within donor, day of flushing, number of flushings, and jack. The identification of these key points is crucial to achieve a higher efficiency of embryo transfer and vitrification processes, before considering their application in the conservation of endangered donkey breeds.

Effect of combined exogenous progesterone with luteotrophic support via equine chorionic gonadotrophin (eCG) on corpus luteum development, circulating progesterone concentrations and embryo development in cattle

2016 ◽  
Vol 28 (3) ◽  
pp. 269 ◽  
Author(s):  
L. O'Hara ◽  
N. Forde ◽  
P. Duffy ◽  
F. Randi ◽  
A. K. Kelly ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Embryo Quality ◽  
Single Injection ◽  
Chorionic Gonadotrophin ◽  
Serum Progesterone ◽  
Conceptus Development ◽  
Luteal Tissue ◽  
Exogenous Progesterone ◽  
Intravaginal Device

The aim was to examine the effect of a single intramuscular (i.m.) injection of equine chorionic gonadotrophin (eCG) on Day 3 after oestrus on corpus luteum (CL) development, circulating progesterone and conceptus development in cross-bred beef heifers. In Experiment 1, heifers received: (1) saline, or a single i.m. injection of eCG on Day 3 at (2) 250 IU (3) 500 IU (4) 750 IU or (5) 1000 IU. Administration of eCG resulted in increased luteal tissue area and progesterone and oestradiol concentrations compared with controls. In Experiment 2, heifers received (1) a progesterone-releasing intravaginal device (PRID Delta) from Day 3 to 5 or (2) a PRID Delta from Day 3 to 5 plus a single injection of 750 IU eCG on Day 3. In vitro-produced blastocysts (n = 10 per recipient) were transferred on Day 7 and heifers were slaughtered on Day 14 to assess conceptus development. Administration of eCG reduced the number of short cycles (6.3% vs 31.3%) and increased mean luteal tissue weight (P = 0.02). Insertion of a PRID Delta on Day 3 resulted in an elevation (P < 0.05) in serum progesterone until removal on Day 5. Administration of eCG at the time of PRID Delta insertion resulted in higher progesterone levels (P < 0.05) from Day 10 onwards. Conceptus dimensions were not affected. In conclusion, a single injection of eCG on Day 3 increased CL size and progesterone concentrations and, when given in conjunction with a progesterone-releasing device, appeared to reduce the number of short cycles, presumably due to its luteotrophic nature. The implications of the elevated oestradiol concentrations for embryo quality require further study.

183 EXERCISE INFLUENCES EMBRYO QUALITY IN MARES

2010 ◽  
Vol 22 (1) ◽  
pp. 250 ◽  
Author(s):  
R. Smith ◽  
K. Vernon ◽  
J. Gibbons ◽  
D. Kelley ◽  
J. Anton ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Embryo Quality ◽  
Exercise Group ◽  
Quality Score ◽  
Control Group ◽  
Recovery Rates ◽  
Embryo Recovery ◽  
Humid Environment ◽  
Negative Effect ◽  
The Mean

Athletic mares are commonly utilized in an embryo transfer regimen during the breeding season, which allows owners to continue to train and compete with these animals. Exercising mares in a hot and humid environment has proven detrimental to embryo recovery rates. However, the effects of exercise under milder temperatures have not been examined. Our goal was to compare embryo recovery rates and embryo quality scores of mares under mild environmental conditions of 2 differing exercise regimens v. a single control group (n = 5). One treatment group (full exercise) consisted of mares (n = 5) exercised 6 days a week throughout the duration of the project. The partial-exercise group (n = 5) was exercised throughout the duration of the project 7 days a week, with full rest from day of detected ovulation to the proceeding embryo collection attempt. This was to limit any deleterious effects exercise may potentially have on the mare and subsequent early developing equine embryo. Exercise resumed the day after an embryo collection attempt for the partial-exercise group. Exercised mares were given a 1-month training period prior to the beginning of this project and were moderately exercised for 30 min in a mechanical exercise pen (free exerciser). Control mares were managed similarly as the other groups, however were not exercised. Mares were bred via artificial insemination and embryos were nonsurgically collected 7 d post-ovulation. Mean environmental temperature during the duration of this study was 27.1°C with average 60% humidity. Immediately following completion of exercise, mares in the full exercise group had a mean temperature increase of 1.0°C and partial-exercised mares increased 0.9°C. Exercise had a significant effect on embryo quality (P < 0.05, Table 1) but did not influence embryo recovery rate. The mean embryo quality score for the control group was 1.1, whereas the full-exercised group was 1.85 and partial-exercised group was 2.5. It appears from these data that exercise has a negative effect on embryo quality, thus lowering the availability of transferable embryos. Embryos of lower quality (≥3) have been shown to result in poor pregnancy rates in horses. Additionally, rest from ovulation to an embryo collection attempt did not improve embryo quality. Table 1.Embryo recovery rate and quality score of embryos from control and exercised mares

An evaluation of the success of MOET in two breeds of hill sheep maintained under normal systems of hill flock management

1999 ◽  
Vol 69 (2) ◽  
pp. 367-376 ◽  
Author(s):  
F. Bari ◽  
M. Khalid ◽  
W. Haresign ◽  
B. Merrell ◽  
A. Murray ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Embryo Quality ◽  
Survival Rates ◽  
Negative Relationship ◽  
Embryo Survival ◽  
Factors Affecting ◽  
Embryo Recovery ◽  
Multiple Ovulation ◽  
Middle Range ◽  
The Mean

AbstractThis study was undertaken to investigate factors affecting the success of multiple ovulation and embryo transfer (MOET) in Scottish Blackface (no. = 120) and Welsh Mountain (no. = 120) ewes, over a period of 2 years using a laparoscopic procedure for both embryo recovery and transfer. Superovulation was induced with ovine FSH, with 98 to 100% of ewes of both the breeds responding to the treatment. The overall mean superovulatory responses were 15⋅0 (s.e. 0⋅8) and 12⋅5 (s.e. 0⋅7) for Scottish Blackface and 15⋅3 (s.e. 0⋅9) and 12·8 (s.e. 0⋅8) for Welsh Mountain ewes in years 1 and 2, respectively. However, there was a wide degree of variation in superovulatory responses within each breed, with a range of 3 to 29 in Scottish Blackface and 1 to 40 in Welsh Mountain ewes. The mean embryo recovery rate was 71⋅9 (s.e. 3⋅5) % and 69⋅6 (s.e. 3⋅4) % for Scottish Blackface and 57⋅5 (s.e. 4⋅1) % and 60⋅6 (s.e. 3⋅6) % for Welsh Mountain ewes in years 1 and 2, respectively. The mean number of embryos recovered from Welsh Mountain ewes was significantly (P < 0⋅05) lower than that from Scottish Blackface ewes in both years. The lower mean number of embryos recovered in year 2 for both breeds was entirely a reflexion of the lower superovulatory responses in year 2. A significant (P < 0⋅001) relationship was observed between superovulatory response and the number of embryos recovered for both breeds. Some 77% and 72% of Scottish Blackface ewes and 65% and 73% of Welsh Mountain ewes yielded four or more transferable embryos in years 1 and 2, respectively. Neither the mean number nor the mean percentage of transferable embryos per donor ewe differed between breeds or years. A significant (P < 0⋅001) negative relationship was observed between the time of onset of oestrus and both superovulatory response and number of embryos recovered in Scottish Blackface ewes only. Embryo quality was affected by the time of onset of oestrus. In both breeds, the highest proportion of grade 1+2 embryos and the lowest proportion of unfertilized/degenerate embryos occurred in the middle range time, with a reduction in the proportion of grade 1+2 embryos in ewes that came into oestrus either early <19 h) or late (>30 h) after sponge removal. Only one embryo was transferred to each recipient and the embryo survival rates were 76⋅8% and 74⋅6% (Scottish Blackface), and 69⋅6% and 87⋅3% (Welsh Mountain) for years 1 and 2, respectively. Overall the results of this study suggest that MOET is as successful in hill ewes as has been reported for lowland breeds, even without making any major concessions to their hill status.

scholarly journals Hormonal Management for the Induction of Luteolysis and Ovulation in Andalusian Jennies: Effect on Reproductive Performance, Embryo Quality and Recovery Rate

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 143
Author(s):  
Marina Bottrel ◽  
Isabel Ortiz ◽  
Manuel Hidalgo ◽  
María Díaz-Jiménez ◽  
Blasa Pereira ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Reproductive Performance ◽  
Human Chorionic Gonadotropin ◽  
Recovery Rate ◽  
Ovulation Induction ◽  
Embryo Quality ◽  
The Other ◽  
Flushing Rate ◽  
Embryo Recovery ◽  
Embryo Grade

Two prostanglandins (luprostiol, LUP, and dinoprost, DIN) and two ovulation-inducing agents (human Chorionic Gonadotropin, hCG, and deslorelin, DES) were evaluated for luteolysis and estrus induction, and for ovulation induction, respectively, in embryo donor jennies. Twenty-six fertile Andalusian jennies were used. In Experiment 1, jennies (n = 112 cycles) were randomly treated with either LUP or DIN after embryo flushing. In Experiment 2, donors (n = 84 cycles) were randomly treated with either hCG or DES to induce ovulation. No differences were found between prostaglandins for all variables studied (prostaglandin–ovulation interval (POI), interovulatory interval (IOI), embryo recovery rate (ERR), positive flushing rate (PFR) and embryo grade (EG)). The ovulation rate was similar for hCG and DES (60.9% vs. 78.7%). However, the interval to ovulation (ITO) was affected (62.61 ± 7.20 vs. 48.79 ± 2.69 h). None of the other variables studied (ERR, PFR and EG) were affected (p > 0.05), except for embryo quality (p = 0.009). In short, both prostaglandins evaluated are adequate to induce luteolysis and estrus. Both ovulation-inducing agents hastened ovulation, but DES seems to be more effective than hCG. Follicular diameter affected the interval from treatment to ovulation, and high uterine edema was related to low embryo quality.

scholarly journals Relationship between superovulation and embryo production with ovarian follicular population before superovulatory treatment in Brazilian Bergamasca sheep

2021 ◽  
Vol 73 (1) ◽  
pp. 115-122
Author(s):  
A.P.M.L. Kolling ◽  
G.C. Brilhante ◽  
J. Drechmer ◽  
L.M. Santos ◽  
B.D.M. Silva ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Negative Correlation ◽  
Recovery Rate ◽  
Embryo Production ◽  
Positive Correlation ◽  
Embryo Recovery ◽  
Production Animals ◽  
Selection Of

ABSTRACT The objective of this study was to quantify the superovulatory response and embryo production of Brazilian Bergamasca sheep and to evaluate the link to the follicular condition before superovulatory treatment, as a reference for selection of donors with potential for superovulation. Follicular population of twenty-three sheep was evaluated by ultrasound during metestrus phase of the estrous cycle and divided into groups of low, medium and high follicular population. Subsequently, they were synchronized, superovulated with 133mg of pFSH, mated and subjected to embryo collection. The superovulatory response (9.0±3.3 vs 10.7±6.2 vs 13.8±7.1) and embryo production (4.0±3.8 vs 2.6±2.0 vs 1,8±4.0) were similar between groups (P>0.05). There was a positive correlation between the number of follicles during the metestrus phase and the number of corpus luteum with premature regression (PLR) (0.52) and a negative correlation between the recovery rate and PLR (-0.44) (P<0.05). The sheep that presented PLR had more follicles during metestrus (16.9±7.8 vs 12.7±3.2) and lower embryo recovery rate (38.8±29.3 vs 72.2±29.9) than those with functional CLs (P<0.05). Follicular quantification during metestrus phases was unable to identify donors with high embryo production. Animals with PLR had a larger follicular population during metestrus and lower embryo recovery rate.

Sperm DNA fragmentation measured by sperm chromatin dispersion impacts morphokinetic parameters, fertilization rate and blastocyst quality in ICSI treatments

Zygote ◽  
2021 ◽  
pp. 1-8
Author(s):  
Shikai Wang ◽  
Weihong Tan ◽  
Yueyue Huang ◽  
Xianbao Mao ◽  
Zhengda Li ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Embryo Quality ◽  
Fertilization Rate ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Blastocyst Formation ◽  
High Quality ◽  
Morphokinetic Parameters ◽  
Sperm Dna ◽  
Blastocyst Quality

Summary To determine the effects of sperm DNA fragmentation (SDF) on embryo morphokinetic parameters, cleavage patterns and embryo quality, this retrospective study analyzed 151 intracytoplasmic sperm injection (ICSI) cycles (1152 embryos collected) between November 2016 and June 2019. SDF was assessed using sperm chromatin dispersion. The cycles were divided into two groups based on the SDF rate: SDF < 15% (n = 114) and SDF ≥ 15% (n = 37). The embryo morphokinetic parameters, cleavage patterns, and embryo quality were compared between the two groups. The morphokinetic parameters tPNf, t2, t3, t4, t5, t6, and t8 were achieved significantly earlier in the SDF < 15% group compared with in the SDF ≥ 15% group. The fertilization and 2PN rates seemed to be significantly higher in the SDF < 15% group compared with in the SDF ≥ 15% group, while the abnormal cleavage rates were similar. However, a significantly higher rate of chaotic cleavage (CC) was observed in the SDF ≥ 15% group. The D3 high-quality embryo and available embryo rates were similar between the two groups. The blastocyst formation, high-quality blastocyst, and available blastocyst rates in the SDF < 15% group were significantly higher than those in the SDF ≥ 15% group. With an increase in SDF level, the chemical pregnancy, clinical pregnancy and implantation rates tended to decrease, while the miscarriage rate increased. This study demonstrated that SDF ≥ 15% reduces the fertilization rate of ICSI cycles and affects certain morphokinetic parameters. A higher SDF level can also induce a higher rate of CC, with subsequent decreases in the blastocyst formation rate and blastocyst quality.

Leptospirosis and embryo recovery rate in mares: Table 1

2012 ◽  
Vol 170 (2) ◽  
pp. 60-60 ◽  
Author(s):  
A. Pinna ◽  
G. Martins ◽  
W. Lilenbaum
Keyword(s):  
Recovery Rate ◽  
Embryo Recovery

scholarly journals Prediction of fertilization rate and embryo quality based on oocyte morphological characteristics

2009 ◽  
Vol 92 (3) ◽  
pp. S216
Author(s):  
M.D. Salazar ◽  
P. Diaz ◽  
G. Garcia ◽  
J. Sepulveda ◽  
R. Santos ◽  
...  
Keyword(s):  
Embryo Quality ◽  
Morphological Characteristics ◽  
Fertilization Rate

On-farm euthanasia practices and attitudes of commercial meat rabbit producers

2017 ◽  
Vol 181 (11) ◽  
pp. 292-292 ◽  
Author(s):  
Jessica Walsh ◽  
Aaron Percival ◽  
Brian Tapscott ◽  
Patricia V Turner
Keyword(s):  
Response Rate ◽  
Age Groups ◽  
Current Method ◽  
Alternative Methods ◽  
Educational Materials ◽  
Blunt Force Trauma ◽  
Working Together ◽  
Timely Fashion ◽  
Cervical Dislocation ◽  
On Farm

Appropriate and timely on-farm euthanasia is the responsibility of the producer, working together with their herd veterinarian. Unfortunately, validated methods for euthanasia of commercial meat rabbits are lacking and there are few educational materials available for producer training. Because euthanasia must be performed in a timely fashion to minimise suffering, it is critical to ensure that methods used are aesthetic, humane and effective. We surveyed Canadian meat rabbit producers for current on-farm euthanasia practices as well as attitudes towards the methods they employed and thoughts on novel euthanasia techniques. Surveys were distributed with a response rate of 26 per cent (n=26). Blunt force trauma was the most common euthanasia method used (54 per cent), followed by assisted manual cervical dislocation (31 per cent). Half of producers admitted to not having a euthanasia method in place for all age groups of rabbits, instead electing to let sick and injured rabbits die on their own. While some producers reported feeling highly skilled and satisfied with their current euthanasia method, 58 per cent reported concerns with their current method and 42 per cent desired alternative methods to be developed. Responses to additional questions on training and awareness of euthanasia resources indicated that veterinarians are not part of on-farm euthanasia planning for meat rabbits.

Sign in / Sign up

Export Citation Format

Share Document