scholarly journals The Addition of Hyaluronic Acid into Platelet-Rich Fibrin Lysate in Restoration of Senescent Human Dermal Fibroblasts Activities

2018 ◽  
Vol 5 (2) ◽  
pp. 85-92
Author(s):  
Rika Azyenela ◽  
Indah Julianto ◽  
Yohanes Widodo Wirohadidjojo
Keyword(s):  
Hyaluronic Acid ◽  
Cellular Proliferation ◽  
Clinical Signs ◽  
Dermal Fibroblasts ◽  
Proliferation Index ◽  
Serum Starvation ◽  
Human Dermal Fibroblasts ◽  
Collagen Deposition ◽  
Platelet Rich Fibrin ◽  
Tgf Β1

Senescent human dermal fibroblasts had reduced capacity in proliferation and collagen synthesis. It is due to unresponsiveness against transforming growth factor-β1 (TGF-β1) stimulation. Either platelet-rich fibrin (PRF)-lysate or hyaluronic acid (HA) can restore TGF-β1 signaling pathway. To determine whether HA addition to PRF lysate has a better activity than PRF-lysate alone in restoring senescent human dermal fibroblasts (HDFs) activities. HDF isolated from six different human skins was divided into normal HDFs and senescent HDFs which are induced by serum starvation. The senescent groups were then given 50% PRF-lysate and various levels of HA. Amelioration of TGF-β1 signaling was measured by cellular proliferation index and collagen deposition.  Addition of HA into PRF-lysate resulted in a significant increase in proliferation index and collagen deposition index than PRF-lysate alone. The best level of HA for this mixture ranged from 20.83 mM to 41.67 mM. HA in PRF lysate is an excellent candidate material for treating clinical signs related to senescent human dermal fibroblasts.   Ethical permission: This experiment had gain approval from the local ethical committee, Ref: KE/FK/471/EC/2016 dated 17-05-2016.

scholarly journals Bovine vitreous gel can reactivate replicative senescence of human dermal fibroblast

2018 ◽  
Vol 23 (1) ◽  
pp. 48
Author(s):  
Maria Vianny Sansan ◽  
Sunardi Radiono ◽  
Muhamad Eko Irawanto ◽  
Yohanes Widodo Wirohadidjojo
Keyword(s):  
Hyaluronic Acid ◽  
Replicative Senescence ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Dermal Fibroblasts ◽  
Influential Factor ◽  
Proliferation Index ◽  
Potential Candidate ◽  
Chronic Ulcers ◽  
Tgf Β1

The most influential factor in the poor healing of chronic ulcers is replicative senescence of fibroblasts that are unresponsive to TGF-β1 stimulation. Cellular replicative senescence can be induced by cultivating normal human dermal fibroblasts (HDFs) in a serum-starved medium. In addition, increasing microenviroment mechanical forces by hyaluronic acid can ameliorate the TGF-β1 signaling of these senescent cells. One of natural resources of hyaluronic acid is bovine vitreous gel. In order to evaluate the effect of bovine-vitreous gel on replicative senescence of fibroblasts, we used various levels of bovine vitreous gel diluted in Dulbecco’s modified Eagle’s medium to stimulate cellular activities of serum-starved HDFs. Those cellular activities were compared to the control media, standardized hyaluronic acid, and to normal HDFs. Our results show that replicative senescence of HDFs treated with 50% bovine vitreous gel exhibited a significantly higher proliferation index, migration rate, and collagen deposition than those cultured in control media, and they displayed an equal level of cellular activity with the HDFs exposed only to standardized hyaluronic acid. We concluded that bovine vitreous gel can be used to stimulate replicative senescence of HDFs and therefore a potential candidate material to stimulate healing of chronic ulcers.

scholarly journals α-Ionone Protects Against UVB-Induced Photoaging in Human Dermal Fibroblasts

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1804 ◽  
Author(s):  
Tao Tong ◽  
Jinju Park ◽  
Youna Moon ◽  
Wesuk Kang ◽  
Taesun Park
Keyword(s):  
Hyaluronic Acid ◽  
Uv Light ◽  
Acid Synthesis ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Skin Damage ◽  
Smad Pathway ◽  
Naturally Occurring ◽  
Expression Of Genes ◽  
Tgf Β1

Ultraviolet (UV) light-induced wrinkle formation is a major dermatological problem and is associated with alteration in collagen. Here, we investigated the potential of α-ionone, a naturally occurring aromatic compound, in regulation of UVB-induced photoaging in human Hs68 dermal fibroblasts and identified the mechanisms involved. We found that in human dermal fibroblasts, α-ionone inhibited UVB-induced loss of collagen. α-Ionone upregulated the molecules participating in the TGF-β–SMAD pathway (TGF-β1, phospho-SMAD2/3, Col1A1, and Col1A2), but downregulated the molecules involved in the MAPK–AP-1 signaling pathway (phospho-p38, phospho-JNK, phospho-ERK, phospho-c-Fos, phospho-c-Jun, MMP1, MMP3, and MMP9), in human dermal fibroblasts. α-Ionone treatment also increased hyaluronic acid contents, and this effect was accompanied by an upregulation of mRNA expression of genes (HAS1 and HAS2) involved in hyaluronic acid synthesis. Thus, α-ionone is effective in the prevention of UVB-induced decrease of collagen and hyaluronic acid in human dermal fibroblasts. We propose that α-ionone may prove beneficial for the prevention of UV-induced wrinkle formation and skin damage.

scholarly journals Regenerative Effects of Wharton’s Jelly Stem Cells-Conditioned Medium in UVA-Irradiated Human Dermal Fibroblasts

2018 ◽  
Vol 5 (1) ◽  
pp. 45-50
Author(s):  
Yohanes Widodo Wirohadidjojo ◽  
Arief Budiyanto ◽  
Hardyanto Soebono
Keyword(s):  
Stem Cells ◽  
Conditioned Medium ◽  
Cellular Proliferation ◽  
Positive Control ◽  
Dermal Fibroblasts ◽  
Wharton’S Jelly ◽  
Cellular Migration ◽  
Human Dermal Fibroblasts ◽  
Collagen Deposition ◽  
Wharton's Jelly

Background: Ultraviolet A radiation (UVA) can photo-age skin by suppressing the proliferation, migration, and collagen deposition of human dermal fibroblasts (HDFs). This process occurs because UVA light can inhibit the gene expression of the TGF-β receptor in HDFs. Moreover, Wharton’s Jelly Stem Cells-Conditioned Medium (WJSC-CM) is hypothesized to release microvesicles that contain short m-RNA with regenerative properties. Objectives: This study aimed to determine the regenerative properties of WJSC-CM on UVA-Irradiated Human Dermal Fibroblasts (UVA-HDFs) Methods: Passaged fourth of  HDFs obtained from the foreskin of six (11- to 13-year-old) boys were repeatedly irradiated with a total of 10 J/cm2 UVA and treated with various concentrations of WJSC-CM. We used non-irradiated HDFs as positive control. After that, the consumption of TGF-β, cellular proliferation, cellular migration, and collagen deposition of each group were measured and compared. Results: Compared to the non-irradiated groups, the proliferation rates, migration rates, and collagen deposition of UVA-HDFs significantly decreased (p<0.05). WJSC-CM can improve the consumption of TGF-β, proliferation, and cellular migration of UVA-HDFs. However, WJSC-CM failed to improve the collagen deposition of UVA-HDFs (p>0.05). Conclusions: WJSC-CM has regenerative properties and is a candidate material for the treatment of prematurely ageing skin induced by UVA-irradiation.   Ethical permission: This experiment was permitted by the local ethical permission committee Ref:KE/FK/382/EC with permission letter dated 17-04-2013.

CTRP6 inhibits fibrogenesis in TGF-β1-stimulated human dermal fibroblasts

2016 ◽  
Vol 475 (4) ◽  
pp. 356-360 ◽  
Author(s):  
Rong-hui Fan ◽  
Xiu-mei Zhu ◽  
Yao-wen Sun ◽  
Hui-zi Peng ◽  
Hang-li Wu ◽  
...  
Keyword(s):  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Tgf Β1

scholarly journals Nanofiber scaffolds attenuate collagen synthesis of human dermal fibroblasts through TGF-β1/TSG-6 pathway

2019 ◽  
Vol 2 (4) ◽  
pp. 044001 ◽  
Author(s):  
Bei Xie ◽  
Wanzong Zhu ◽  
Pinghui Ding ◽  
Mengting Chen ◽  
Ji Li ◽  
...  
Keyword(s):  
Collagen Synthesis ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Nanofiber Scaffolds ◽  
Tgf Β1

scholarly journals Genistein does not Inhibit TGF-β1-Induced Conversion of Human Dermal Fibroblasts to Myofibroblasts

2021 ◽  
pp. 815-820
Author(s):  
M KAŇUCHOVÁ ◽  
L URBAN ◽  
N MELEGOVÁ ◽  
M ČOMA ◽  
B DVOŘÁNKOVÁ ◽  
...  
Keyword(s):  
Transforming Growth Factor Beta ◽  
Wound Repair ◽  
Transforming Growth Factor ◽  
Dermal Fibroblasts ◽  
Point Of View ◽  
Human Dermal Fibroblasts ◽  
Receptor Modulator ◽  
Naturally Occurring ◽  
Growth Factor Beta ◽  
Tgf Β1

Transforming growth factor beta 1 (TGF-β1) is a pro-fibrotic cytokine with a key role in wound repair and regeneration, including induction of fibroblast-to-myofibroblast transition. Genistein is a naturally occurring selective estrogen receptor modulator with promising anti-fibrotic properties. In the present study we aimed to investigate whether genistein modulates TGF-β1 (canonical and non-canonical) signaling in normal dermal fibroblasts at the protein level (Western blot and immunofluo-rescence). We demonstrated that TGF-β1 induces the myofibroblast-like phenotype in the studied fibroblast signaling via canonical (SMAD) and non-canonical (AKT, ERK1/2, ROCK) pathways. Genistein induced only ERK1/2 expression, whereas the combination of TGF-β1 and genistein attenuated the ERK1/2 and ROCK signaling. Of note, the other studied pathways remained almost unaffected. From this point of view, genistein does not impair conversion of normal fibroblasts to myofibroblast-like cells.

scholarly journals Ginseng Gintonin Enhances Hyaluronic Acid and Collagen Release from Human Dermal Fibroblasts Through Lysophosphatidic Acid Receptor Interaction

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4438 ◽  
Author(s):  
Rami Lee ◽  
Na-Eun Lee ◽  
Hongik Hwang ◽  
Hyewhon Rhim ◽  
Ik-Hyun Cho ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Receptor Antagonist ◽  
Lysophosphatidic Acid ◽  
Dermal Fibroblasts ◽  
Time Dependent ◽  
Receptor Subtypes ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Human Dermal Fibroblasts ◽  
Lpa Receptors

Gintonin is a newly discovered component of ginseng and acts as a ligand for G protein-coupled lysophosphatidic acid (LPA) receptors. It is currently unclear whether gintonin has skin-related effects. Here, we examined the effects of a gintonin-enriched fraction (GEF) on [Ca2+]i transient induction in human dermal fibroblasts (HDFs). We found that GEF treatment transiently induced [Ca2+]i in a dose-dependent manner. GEF also increased cell viability and proliferation, which could be blocked by Ki16425, an LPA1/3 receptor antagonist, or 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), a calcium chelator. We further found that GEF stimulated hyaluronic acid (HA) release from HDFs in a dose- and time-dependent manner, which could be attenuated by Ki16425, U73122, a phospholipase C inhibitor, 2-Aminoethoxydiphenyl borate (2-APB), an IP3 receptor antagonist, and BAPTA-AM. Moreover, we found that GEF increased HA synthase 1 (HAS1) expression in a time-dependent manner. We also found that GEF stimulates collagen release and the expression of collagen 1, 3, and 7 synthases in a time-dependent manner. GEF-mediated collagen synthesis could be blocked by Ki16425, U73122, 2-APB, and BAPTA-AM. GEF treatment also increased the mRNA levels of LPA1-6 receptor subtypes at 8 h and increased the protein levels of LPA1-6 receptor subtypes at 8 h. Overall, these results indicate that the GEF-mediated transient induction of [Ca2+]i is coupled to HA and collagen release from HDFs via LPA receptor regulations. We can, thus, conclude that GEF might exert a beneficial effect on human skin physiology via LPA receptors.

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