scholarly journals The effect of subchronic inhalations of nitric oxide on metabolic processes in blood of experimental animals

2016 ◽  
Vol 62 (2) ◽  
pp. 212-214 ◽  
Author(s):  
A.G. Soloveva ◽  
S.P. Peretyagin
Keyword(s):  
Nitric Oxide ◽  
Lactate Dehydrogenase ◽  
Aldehyde Dehydrogenase ◽  
Catalase Activity ◽  
Wistar Rats ◽  
Aldh Activity ◽  
Metabolic Processes ◽  
Experimental Animals ◽  
Ldh Activity

Metabolic processes were investigated in plasma and erythrocytes of Wistar rats exposed to daily 10-min sessions of NO inhalation for 30 days. These included determination of glucose and lactate, catalase activity, and activities of aldehyde dehydrogenase (ALDH), lactate dehydrogenase (LDH), and catalase. NO inhalation in a concentration of 20 ppm, 50 ppm and 100 ppm caused an increase in glucose and lactate. Inhalation of 100 ppm NO also increased catalase activity. Inhalation of all NO concentrations resulted in a decrease of ALDH activity, while the decrease in LDH activity was observed at NO concentrations of 50-100 ppm

scholarly journals Anti-Protozoan Activities of Stachytarpheta angustifolia on Some Haematological Parameters in Wistar Rats

2021 ◽  
Vol 3 (2) ◽  
pp. 6-11
Author(s):  
Murtala Muhammad Abdu ◽  
Y. Sadau ◽  
S.O. Oladejo ◽  
A.M. Yusuf ◽  
M.S. Muhammad ◽  
...  
Keyword(s):  
Analysis Of Variance ◽  
Extraction Method ◽  
Wistar Rats ◽  
Harmful Effect ◽  
Herbal Extract ◽  
Haematological Parameters ◽  
Experimental Animals ◽  
Minimal Stress ◽  
Powdered Form

This study focuses on the anti-protozoan activities of Stachytarpheta angustifolia (Tarkajiya; Hausa, Devil’s coach whip; English) on haematological parameters of Albino Wistar rats which is an unexplored study area. The work is aimed at the determination of the effects of S. angustifolia on Wistar Rats, when exposed to herbal extract on the haematological parameters of Wistar Rats infected with E. tenella Biomarkers. The plant was obtained whole; dried under the shade, made into a powdered form and aqueous extraction method carried by maceration technique. After infecting the experimental animals with the parasites; E. tenella, the following respective doses of 750 mg and 1500 mg were administered to the rats in groups of 3 and 4. Results obtained were analyzed using Analysis of Variance (ANOVA). It was discovered that no significant harmful effect on the rats was recorded, but 60 % of the parasites were killed. This work demonstrated that the herbal extract killed the parasites but induced minimal stress to the animals as shown by the low haematological parameters in the study.

Lactate dehydrogenase activity as measured by the SMAC is related to flow-cell energy.

1982 ◽  
Vol 28 (10) ◽  
pp. 2106-2109 ◽  
Author(s):  
E A Robertson ◽  
E Wright ◽  
R A Chesler ◽  
R J Elin
Keyword(s):  
Lactate Dehydrogenase ◽  
Light Intensity ◽  
Fiber Optic ◽  
Lactate Dehydrogenase Activity ◽  
Flow Cells ◽  
Ldh Activity ◽  
Cell Energy ◽  
The Difference ◽  
Calibration Material

Abstract Determination of lactate dehydrogenase (LDH) activity in the SMAC (Technicon) is based on the change in NADH absorbance between two flow cells. We noted that results for patients' specimens and controls changed when the fiber optic terminations for the two LDH channel flow cells were adjusted or "peaked" at the colorimeter chopper assembly. The energy (intensity) of light reaching the flow cells was varied by adjusting the fiber optic terminations, and the absorbance readings for a series of solutions containing NADH and patients' specimens were recorded. For both flow cells, when the fiber optic terminations were adjusted to increase the zero absorbance light intensity from 20 lines to 60 lines, a significant (p less than 0.0001) proportional change was seen in the absorbance readings. Evidently the difference in absorbance between the two flow cells is related not only to the NADH concentrations but also to the difference in the light intensity at the two flow cells. Consequently, changes in the adjustment of the fiber optic terminations produce systematic changes in results for LDH in patients' sera. These systematic changes in LDH results may be minimized by maintaining equivalent settings of the fiber optic terminations for the two flow cells and by using the calibration material with an absorbance most similar to that of patients' specimens.

Optimized Spectrophotometric Determination of Aldehyde Dehydrogenase Activity in Erythrocytes

1992 ◽  
Vol 38 (4) ◽  
pp. 584-588 ◽  
Author(s):  
R D Johnson ◽  
J Bahnisch ◽  
B Stewart ◽  
D J Shearman ◽  
J B Edwards
Keyword(s):  
Alcohol Consumption ◽  
Dehydrogenase Activity ◽  
Aldehyde Dehydrogenase ◽  
Biological Marker ◽  
Aldh Activity ◽  
Lactate Dehydrogenase Activity ◽  
Analytical Imprecision ◽  
Excess Alcohol Consumption ◽  
Aldehyde Dehydrogenase Activity

Abstract We describe a reliable and sensitive semiautomated spectrophotometric assay of aldehyde dehydrogenase (ALDH; EC 1.2.1.3) activity in erythrocytes. The hemolysate can be stabilized with sucrose, and the technique involves only microliters of hemolysate on a centrifugal analyzer. The use of microcolumns to remove interfering hemoglobin is avoided, and reproducibility of the assay has been improved by manipulating the inherent lactate dehydrogenase activity of erythrocytes by adding lactate and oxalate to the reaction mixture. These modifications have decreased the analytical imprecision of the assay, allowing a better appraisal of aldehyde dehydrogenase activity in erythrocytes as a biological marker of excess alcohol consumption. Erythrocytic ALDH activity was significantly less in 40 alcoholics than in 145 teetotallers (median activity 128 vs 219 mU/g of hemoglobin, respectively; P = 0.0001), indicating the potential of this assay as a useful marker of excess alcohol consumption.

scholarly journals Biochemical Effects of Aqueous Extract of Persea americana (Mill) on the Myocardium of Left Ventricle of High Salt–Fed Adult Wistar Rats

2017 ◽  
Vol 22 (4) ◽  
pp. 765-769
Author(s):  
Ayoola I. Olushola ◽  
Komolafe O. Aderibigbe ◽  
Saka O. Stephen ◽  
Odukoya S. Ayodeji
Keyword(s):  
Nitric Oxide ◽  
Lactate Dehydrogenase ◽  
Wistar Rats ◽  
Potassium Ion ◽  
Persea Americana ◽  
High Salt ◽  
Sodium Ion ◽  
Ion Concentration ◽  
High Salt Diet ◽  
Salt Diet

Background. The cardioprotective effects of Persea americana extract was investigated on biochemical activities of high salt–fed adult Wistar rats in this study. Method. Forty healthy Wistar rats of both sexes weighing 120 to 150 g were randomly assigned into 8 groups of 5 rats each (groups A, B, C, D, E, F, G, and H). Rats in groups A, F, G, and H were fed with standard laboratory pellets, while groups B, C, D, and E were fed on the high-salt diet for 4 weeks. Concomitantly, daily administration of 50, 100, and 150 mg/kg of the P americana extract were given orally to groups C and F, D and G, and E and H, respectively, while rats in groups A and B were administered distilled water. Blood samples were taken by cardiac puncture; concentration of sodium ion, potassium ion, nitric oxide, and activity of lactate dehydrogenase were determined. One-way analysis of variance was used to analyze data, followed by Student-Newman-Keuls (SNK) test for multiple comparison. Results. Results revealed that concentration of potassium ion and nitric oxide was significantly lower ( P < .05) in high salt–fed groups. Sodium ion concentration and activity of lactate dehydrogenase were higher in high salt–fed group while P americana prevented biochemical perturbations in other experimental groups. Conclusion. In conclusion, high salt–diet induced biochemical alterations which were significantly protected by oral administration of P americana extract.

Aldehyde dehydrogenase in fresh primordial germ cells as a marker of cell ‘stemness’

Zygote ◽  
2019 ◽  
Vol 27 (1) ◽  
pp. 46-48
Author(s):  
Andrea Svoradová ◽  
Jaromír Vašíček ◽  
Alexander Ostró ◽  
Peter Chrenek
Keyword(s):  
Aldehyde Dehydrogenase ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Specific Inhibitor ◽  
Cell Types ◽  
Functional Marker ◽  
Aldh Activity

SummaryChicken primordial germ cells (PGCs) are the primary pluripotent stem cell types that will differentiate towards germ cells. High aldehyde dehydrogenase (ALDH) activity is considered as a functional marker for the detection of cell ‘stemness’. In our study the ALDEFLUOR™ kit was used for determination of ALDH activity in PGCs. PGCs were co-stained with diethylaminobenzaldehyde (DEAB) and ALDH and analyzed by flow cytometry. Our results showed a small cell population (8.0 ± 3.3%) upon preincubation of the cells with the specific inhibitor DEAB, however cells without inhibitor staining showed a fluorescence shift as an ALDH-positive population (70.5 ± 1.6%). These findings indicate higher expression of ALDH in PGCs and ALDH activity can therefore be used as a new functional marker for the detection of cell ‘stemness’ in chicken PGCs. These results may have importance for characterization of PGCs as a potential genetic resource in poultry. Further research is necessary to elucidate the role of this functional marker in these cells.

Determination of lactate dehydrogenase (LDH) activity in milk by a fluorometric assay

2005 ◽  
Vol 72 (2) ◽  
pp. 209-216 ◽  
Author(s):  
Torben Larsen
Keyword(s):  
Lactate Dehydrogenase ◽  
Large Scale ◽  
Raw Milk ◽  
Milk Samples ◽  
Ldh Activity ◽  
Straight Line ◽  
Methodological Problems ◽  
Assay Precision ◽  
Detailed Kinetics

Indigenous L-lactate dehydrogenase (LDH) in milk originates mainly from somatic cells, leucocytes and invading microorganisms. Its activity may be used for detection of mastitis. However, existing methods to measure LDH activity in milk both need pretreatment of the samples and still suffer from methodological problems. The present paper describes a fast, reliable method for determination of LDH activity, suitable for milk samples. The method is based on fluorometric determination of enzyme kinetics when L-lactate is converted to pyruvate. The assay uses raw milk without pretreatment and the method is easily adjustable to large-scale analyses on micro assay plates. Detection is based on (straight line) linear response within 4–7 min of initiation of the reaction. A substrate concentration of 35 mM in the reaction mixture was considered to be optimal for the assay. Intra plate assay precision was approx. 6% (CV) and the inter plate precision approx. 10%. Known inhibitors of LDH activity (oxidative direction), i.e., oxalic acid, oxamate, and pyruvate, were tested in different concentrations in order to verify the specificity of the response. The detailed kinetics of samples analysed indicated that the isoenzyme composition may have differed between milk samples, and that this composition may have been altered in high activity samples.

Analysis of Fibronectin and TGF-β1 Immunoexpression to Determination of Wound Vitality in Wistar Rats (Rattus norvegicus)

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Nita Novita ◽  
Hasrayati Agustina ◽  
Bethy S. Hernowo ◽  
Abdul H. Hassan
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Age Determination ◽  
Scientific Field ◽  
Wound Age ◽  
Forensic Practice ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tgf Β1

Wound examination is indispensable in forensic practice. The scientific field of wound age determination has advanced progressively during recent years.The purpose of this study was to determine the differences of fibronectin and TGF-β1 expression in both antemortem and postmortem wounds. This study was an experimental with completely randomized design.  The skin wounds (vital and postmortem) were taken from fourty Wistar rats and divided into 10 groups of rats. Immunohistochemical staining was performed to determine the differences between antemortem and postmortem wounds. The result showed that in 30 minutes after antemortem wound infliction, all of samples showed weak reactivity for fibronectin and TGF-β1 (100%).  In first hour after wound infliction, 3 samples (75%) showed weakly positive and 1 sample (25%) strongly positive for fibronectin and TGF-β1.  In 2 hour after wound infliction, 1 sample (25%) showed weakly positive and 3 sample (75%) strongly positive for fibronectin and TGF-β1.  In 3 and 4 hour after wound infliction, all of samples strongly positive for fibronectin and TGF-β1.  In postmortem wound, all of samples showed negativity for fibronectin and TGF-β1. In conclusion, fibronectin and TGF-β1 may be useful in the determination of wound vitality. Keywords: wound, fibronectin, TGF-β1, vitality

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