Diagnostic capabilities of the biochemical composition of amniotic fluid in assessing fetal conditions

Aim. To analyze the nature of changes in the biochemical parameters of amniotic fluid during fetal hypoxia. Methods. The study was carried out in the maternity ward of the Republican Clinical Hospital named after Kuvatov (Ufa) between January 2016 and September 2018. The main group 72 women in labor with symptoms of fetal distress, the control group 70 women in labor without it. The biochemical composition of the amniotic fluid was measured using an analyzer. Statistical analysis was performed by using the Statistica 10.0 software. Comparison of qualitative characteristics was carried out by using Fisher's exact test when comparing quantitative data, the MannWhitney test. The statistical significance of the differences was set at p 0.05. Results. It was found that in fetal distress, there is a decrease in such biochemical indicators of the composition of amniotic fluid as the level of triglycerides (0.20.1 and 0.30.1 mmol/L, p=0.0036) and cholesterol (0.10.16 and 0.30.2 mmol/L, p=0.0275), gamma-glutamyl transpeptidase activity (34.511 and 48.76.8 U/L, p=0.0261), while the level the lactate (in the main group 3.51.2 and 3.10.9 mmol/L in the control group, p=0.0035), glucose (1.20.6 and 0.60.3 mmol/L, p=0.0002) and nitrogenous substances such as urea (4.51.1 and 3.01.3 mmol/L, p=0.0018) increases. Conclusion. The biochemical composition of amniotic fluid reflects the state of the fetus at birth, and therefore the study of the amniotic fluid is a relevant and accessible method.

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Association between 10 Microsatellite Markers and Nonsyndromic Cleft Lip Palate in the Chilean Population

The Cleft Palate-Craniofacial Journal ◽

10.1597/02-147 ◽

2004 ◽

Vol 41 (2) ◽

pp. 163-167 ◽

Cited By ~ 13

Author(s):

Rafael Blanco ◽

José Suazo ◽

JoséLuis Santos ◽

Mónica Paredes ◽

Hsiao Sung ◽

...

Keyword(s):

Cleft Lip ◽

Statistical Significance ◽

Positive Family History ◽

Case Control ◽

University Hospital ◽

Control Group ◽

Exact Test ◽

Rehabilitation Centers ◽

Cleft Lip Palate ◽

Chilean Population

Objective The objective of this case-control study was to evaluate the possible association between nonsyndromic cleft lip/palate (NSCLP) and 10 genetic markers in four chromosomal regions in the admixed Spanish-Amerindian Chilean population. Setting Study participants included 56 patients with NSCLP identified and interviewed for positive family history during the course of clinical examinations at different rehabilitation centers in the cities of Santiago and Talca, Chile. A control group of 59 normal individuals without known familial antecedents of clefting was obtained from blood bank donors of the University Hospital, University of Chile. Cases and controls belonged to low- to low-middle socioeconomic strata. Results Ten markers from chromosome 4p, 4q, 6p, 17q, and 19q were assessed (MSX1, D4S175, D4S192, F13A1, EDN1, D6S89, D6S105, D6S109, D17S579, BCL3). Four of them showed significant deviations from Hardy-Weinberg expectations in controls, according to the exact test (D4S192, BCL3, F13A1, and D6S89). The case-control comparison by means of the CLUMP program showed significant differences only in BCL3, and D6S109 almost reached statistical significance. Conclusions Most of the genetic regions with positive results in Caucasian populations may not be involved in NSCLP in Chile, regardless of the positive evidence for the candidate region on chromosome 19. Similar findings have been reported recently in the Chinese population.

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Determining the risk factors for xerostomia in dental out-patients

Kazan medical journal ◽

10.17750/kmj2015-174 ◽

2015 ◽

Vol 96 (2) ◽

pp. 174-177

Author(s):

K V Komarova ◽

N N Ratkina ◽

V K Polenichkin ◽

E P Karmanov

Keyword(s):

Risk Factors ◽

Musculoskeletal Diseases ◽

Statistical Significance ◽

Main Group ◽

Control Group ◽

Endocrine Disorders ◽

Chi Square ◽

Chi Square Test ◽

Period Duration ◽

Group 1

Aim. To determine the risk factors for xerostomia.Methods. The study included 137 patients aged 25 to 60 years (61 males, 76 females), the main group consisted of 40 patients with xerostomia (11 males, 29 females), with a median age of 44.5 years (38; 49.5). The control group included 97 patients without xerostomia (50 males, 47 females), median age - 42 years (36; 49). The questionnaire by V.V. Afanas’ev (1993) was used to collect the complaints, anamnesis and to determine the risk factors for xerostomia. Qualitative indicators are listed as absolute numbers and shares (%). In assessing the statistical significance of qualitative indicators differences, contingency tables with subsequent Pearson chi-square test were used.Results. Among the patients of the main group, 19 (47.5%) took medications decreasing the salivation, with intake period duration ranging from 1 to 4 months. When questioned, patients with xerostomia reported statistically significant increase in the rates of cardiovascular disorders (15 patients, 37.5%), gastrointestinal tract diseases (11 patients, 27.5%), endocrine diseases (10 patients, 25%), musculoskeletal diseases (9 patients, 22.5%). The combination of three or more positive answers on V.V. Afanas’ev questionnaire questions was revealed in 12 (30%) patients of the main group, which was significantly higher compared to the control group (1 patient, 1.03%).Conclusion. The most typical reasons for developing xerostomia were the following: taking medications decreasing the salivation, presence of cardiovascular, gastrointestinal, musculoskeletal and endocrine disorders. Three or more positive answers on V.V. Afanas’ev questionnaire questions increase the probability of detecting xerostomia in a respondent.

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The effectiveness of kinesio taping in edema-reducing treatment for fractures of the lower jaw

Kazan medical journal ◽

10.17816/kmj2021-243 ◽

2021 ◽

Vol 102 (2) ◽

pp. 243-248

Author(s):

D K Gasimzade ◽

F V Takhavieva ◽

S S Ksembaev ◽

O A Ivanov

Keyword(s):

Statistical Significance ◽

Early Postoperative Period ◽

Main Group ◽

Control Group ◽

Mandibular Fractures ◽

Kinesio Taping ◽

Lower Jaw ◽

Measured Length ◽

Miniplate Osteosynthesis ◽

Angle Fractures

Aim. To assess the effect of Kinesio taping on edema reduction among the patients with fractures of the mandible (lower jaw) in the early postsurgical period of osteosynthesis. Methods. Patients with unilateral mandibular angle fractures with displacement of fragments (49 men at the age of 1850 years), divided into 2 groups: the main group 26 patients (with the inclusion of Kinesio taping in the complex of treatment) and the control group 23 patients (the traditional complex of treatment). All patients underwent splinting of the jaw and miniplate osteosynthesis. Kinesio tapes were applied the next day after surgery to the skin above and below the surgical wound. Three out of five standard landmarks were used to measure edema on the 1st and 5th days after osteosynthesis. Data were analyzed by using descriptive statistics and Student's test. In all cases, the level of statistical significance was 5% (p 0.05). Results. On the day after osteosynthesis, the patients of the main group showed a statistically significant (р 0.003) increase in the measured length for all three landmarks compared with the intact side due to pronounced postoperative edema. On the 5th day after the inclusion of Kinesio taping in the complex of treatment, there was a reduction in edema the indicators of the fractured and intact sides did not differ significantly from each other (р 0.05). In patients of the comparison group, the decrease in the parameters of postoperative edema on the 5th day of observation was negligible and insignificant in all measurements (р 0.05). Conclusion. The results indicate the effectiveness of using Kinesio taping for mandibular fractures in the early postoperative period of osteosynthesis, which makes it possible to achieve a significant reduction in postoperative edema; Kinesio taping is a promising, easy-to-perform method that can be used to reduce inflammatory edema in fractures of the mandible.

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Diagnostic capabilities of different methods of laser doppler flowmetry spectral indexes assessment in patients with diabetic microangiopathy

Biomedical Photonics ◽

10.24931/2413-9432-2021-10-2-18-24 ◽

2021 ◽

Vol 10 (2) ◽

pp. 18-24

Author(s):

P. V. Vasilev ◽

N. P. Erofeev ◽

A. N. Shishkin

Keyword(s):

Diabetes Mellitus ◽

Laser Doppler Flowmetry ◽

Main Group ◽

Laser Doppler ◽

Diabetic Microangiopathy ◽

Control Group ◽

Spectral Parameters ◽

Doppler Flowmetry ◽

Diagnostic Capabilities ◽

And Control

The article contains the results of a study of two different methods for calculating the spectral parameters of laser Doppler flowmetry in patients with diabetic microangiopathy caused by type 2 diabetes mellitus (main group) and those with excluded diabetes mellitus (control group). Spectral indices were calculated using either average or maximum amplitudes of the frequency ranges. When comparing the contribution of respiratory and pulse fluxmotions using average amplitudes, there were significant (p < 0.05) differences between the main and control groups. On the contrary, when using the maximum amplitudes, no significant differences were noted (p > 0.05). Also, significant correlations were found between the contributions of respiratory and pulse fluxmotions and the estimated glomerular filtration rate in the main group, using both calculation methods. These studies indicate the feasibility of using a technique based on the analysis of average amplitudes to increase the specificity of laser Doppler flowmetry as a method for diagnosing diabetic microangiopathy.

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Acute hypoxic hypoxia increases lactate concentration in amniotic fluid of rabbits on 27-28th day of pregnancy

Biomeditsinskaya Khimiya ◽

10.18097/pbmc20176301081 ◽

2017 ◽

Vol 63 (1) ◽

pp. 81-84

Author(s):

A.V. Popovtseva ◽

E.V. Suzopov ◽

Yu.V. Korenovsky

Keyword(s):

Amniotic Fluid ◽

Lactate Level ◽

Biochemical Composition ◽

Lactate Concentration ◽

Late Pregnancy ◽

Hypoxic Hypoxia ◽

Control Group ◽

Acute Hypoxic Hypoxia ◽

And Control ◽

High Lactate

We evaluated the influence of hypoxic hypoxia on lactate, creatinine and urea concentrations in the amniotic fluid (AF) of rabbits on 27-28th day of pregnancy. Rabbits were randomly sudivided into two groups: experimental (n=9) and control (n=6). Rabbits of experimental groups were placed in a hypoxic chamber containing 10±2% oxygen and 90±2% nitrogen for 1 h and then were euthanized, AF was extracted from the amniotic sacs via disposable syringe. Acute hypoxic hypoxia had no effect on the AF volume, increased (1.4-fold) lactate, (1.3-fold) creatinine and (1.1-fold) urea concentrations in AF. In contrast to animals of the control group, lactate concentration in the groups with hypoxic hypoxia correlated with the creatinine (r=0.71, p<0.0001, n=35) and urea concentrations in the AF (r=0.81, p<0.0001, n=35). These results suggest that acute hypoxic hypoxia in late pregnancy causes changes in the biochemical composition of AF; these changes are characterized by high lactate concentrations, and the fetus and uterus can be the source of increased lactate level in AF.

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Epidemiology of GB Virus C/Hepatitis G Virus Infection in Patients on Peritoneal Dialysis

Peritoneal Dialysis International ◽

10.1177/089686080202200317 ◽

2002 ◽

Vol 22 (3) ◽

pp. 405-410 ◽

Cited By ~ 5

Author(s):

Fabrizio Fabrizi ◽

Amedeo F. Vecchi De ◽

Giovanna Lunghi ◽

Silvia Finazzi ◽

Sergio Bisegna ◽

...

Keyword(s):

Peritoneal Dialysis ◽

Blood Donors ◽

Statistical Significance ◽

Chronic Hemodialysis ◽

Control Group ◽

Rt Pcr ◽

Gamma Glutamyl Transpeptidase ◽

Hepatitis G Virus ◽

The Difference ◽

Virus C

Background A new genus in the family Flaviviridae has recently been discovered; it has provisionally been designated GBV-C/HGV. As determined by virologic techniques [reverse-transcription polymerase chain reaction (RT-PCR)], infection with GBV-C/HGV is frequent in renal transplant (RT) recipients and in patients on chronic hemodialysis (HD). The epidemiology of GBV-C/HGV infection in patients on peritoneal dialysis is scarce and mostly based on RT-PCR technology. Purpose We report on the prevalence (as detected by serologic and virologic techniques) and the risk factors for GBV-C/HGV infection in a cohort of patients on continuous ambulatory peritoneal dialysis (CAPD). We also tested a control group of blood donors. Methods Infection by GBV-C/HGV was assessed by serologic and virologic techniques. Cases of GBV-C/HGV viremia (GBV-C/HGV RNA) were detected by RT-PCR. Antibodies to the envelope protein of GBV-C/HGV (anti-E2 GBV-C/HGV antibody) were analyzed by serologic methods. Results We found a high frequency [17/85 (20%)] of GBV-C/HGV. The rates of GBV-C/HGV viremia and anti-E2 GBV-C/HGV positivity were 10.5% (9/85) and 10.5% (9/85) respectively. In most patients [17/18 (94%)], the presence of anti-E2 GBV-C/HGV antibody was associated with clearance of GBV-C/HGV from serum. No relationship was noted between anti-E2 GBV-C/HGV antibody (or GBV-C/HGV viremia) and age, sex, race, time on dialysis, anti-HCV antibody, HBsAg status, and anti-HIV positivity. The frequency of GBV-C/HGV infection in CAPD patients was much higher than that in blood donors, even if the difference did not approach statistical significance. No associations between GBV-C/HGV positivity and biochemical liver tests [aminotransferase and gamma glutamyl transpeptidase (GGT)] were apparent. Conclusions Infection by GBV-C/HGV as detected by RT-PCR and anti-E2 antibody was common in patients on CAPD and in controls alike. No association was seen between GBV-C/HGV and various demographic or clinical factors. The clinical significance of GBV-C/HGV in CAPD remains unclear. Larger investigations are in progress.

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Study of effects of oligohydramnios on perinatal outcome

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20202342 ◽

2020 ◽

Vol 9 (6) ◽

pp. 2525

Author(s):

Aashka M. Mashkaria ◽

Babulal S. Patel ◽

Akshay C. Shah ◽

Shashwat K. Jani ◽

Vismay B. Patel ◽

...

Keyword(s):

Caesarean Section ◽

Amniotic Fluid ◽

Perinatal Outcome ◽

Stress Test ◽

Fetal Distress ◽

Control Group ◽

Study Group ◽

Amniotic Fluid Index ◽

Lower Segment Caesarean Section ◽

And Control

Background: Oligohydramnios has got a noteworthy influence on perinatal outcome. Hence, early detection and its timely management will aid in curtailing of perinatal morbidity and mortality and leading to decreased operative interventions. Therefore, the present study is conducted to look for the effects of oligohydramnios.Methods: This comparative study was a prospective observational study conducted at study institution. The women were divided into study and control groups based on AFI (amniotic fluid index), 100 cases were selected in each group.Results: Out of the 200 women, included in the present study, 35% of the patients in the study group had non-reactive non-stress test (NST) while in the control group 7% had it. Caesarean section was performed in 58% of cases in the study group as compared to 30% in the control group. Amongst these, Fetal distress was the most common indication for LSCS (lower segment caesarean section). There were no perinatal deaths in this study.Conclusions: Based on this study it has been observed that, amniotic fluid index of ≤5 cm was commonly associated with increased LSCS rates, intrauterine growth restriction, non-reactive NST, and abnormal Doppler velocimetry studies. Therefore, every case of oligohydramnios requires to be assessed meticulously. Prompt detection; timely management and treating the underlying condition improve outcome.

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Features of Pregnancy and Characteristics of Obstetric Pathology in Women with Obesity of Various Degrees and Physiological Weight

Ukraïnsʹkij žurnal medicini bìologìï ta sportu ◽

10.26693/jmbs05.06.182 ◽

2020 ◽

Vol 5 (6) ◽

pp. 182-187

Author(s):

K. V. Tarasenko ◽

Keyword(s):

Body Weight ◽

Pregnant Women ◽

Statistical Significance ◽

Asymptomatic Bacteriuria ◽

Fetal Distress ◽

Control Group ◽

Obese Women ◽

Social Significance ◽

Obstetric Hemorrhage ◽

Placental Dysfunction

The medical and social significance of obesity in pregnant women lies in the high frequency of obstetric complications: miscarriage, preeclampsia, placental dysfunction, abnormal labor, obstetric hemorrhages, infectious and extragenital diseases in offspring that are programmed in utero. The purpose of the research was to analyze the course of pregnancy and to characterize obstetric pathology in women with obesity of various degrees and physiological body weight. Material and methods. To achieve this goal, we surveyed 369 pregnant women. Pregnant women with obesity were divided into three experimental groups: the first one consisted of 149 women with obesity of the 1st degree; the second group included 73 women with the 2nd degree obesity; the third group had 46 women with the 3rd degree obesity. The control group consisted of 101 women with physiological body weight (BMI = 18.5-25 kg/m2). Results and discussion. In the early term of pregnancy, the risk of miscarriage in women with obesity of different degrees was reliably higher by 2.1-2.9 times compared to the control group (p˂0.05). The risk of late miscarriage in groups of women with obesity of different degrees was 1.7-2.4 times higher than in the control group, although these differences were unreliable. There was also a clear tendency for the risk of preterm labor to increase by 1.4-1.7 times (p˃0.05) in obese women. There was a significant increase in the frequency of placental dysfunction in pregnant women with obesity of varying degrees, which was 1.3-1.8 times higher than the control values. An increase in the incidence of placental dysfunction in obese pregnant women manifested as an increase in the number of cases of fetal distress during pregnancy, the frequency of which increased in accordance with the severity of obesity. There was an increase in the incidence of early preeclampsia without statistical significance in women with the 2nd and 3rd degree obesity. Regarding preeclampsia, a significant increase in its frequency should be noted in obese pregnant women depending on the severity. Only women with 2nd and 3rd degree obesity had obstetric hemorrhage caused by premature detachment of the normally situated placenta. This complication was not observed in pregnant women of the control group and with the I degree obesity. Anemia of pregnancy was 1.5 times more common in women with I degree obesity and 1.4 times in women with the 2nd degree obesity, compared to the control group. In obese pregnant women, the frequency of asymptomatic bacteriuria increased in the 1st degree obesity by 1.6 times, in the 2nd and 3rd degrees by 1.2 and 1.4 times respectively compared to the control group. Conclusion. Thus, obesity is an aggravating factor of pregnancy, as evidenced by the increasing frequency of the most threatening complications for the mother and fetus such as miscarriage, placental dysfunction, preeclampsia, and obstetric hemorrhage compared to pregnant women with physiological body weight

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Possible Protective Effect of LOXL1 Variant in the Cohort of Chernobyl Catastrophe Clean-Up Workers

Genes ◽

10.3390/genes12081231 ◽

2021 ◽

Vol 12 (8) ◽

pp. 1231

Author(s):

Gabrielė Žukauskaitė ◽

Ingrida Domarkienė ◽

Aušra Matulevičienė ◽

Evelina Marija Vaitėnienė ◽

Justas Arasimavičius ◽

...

Keyword(s):

Linkage Disequilibrium ◽

Statistical Significance ◽

Microarray Dataset ◽

Genomic Variation ◽

Control Group ◽

Common Disease ◽

Exfoliation Syndrome ◽

Reference Allele ◽

Exact Test ◽

Epistasis Analysis

Ionising radiation (IR) is an environmental factor known to alter genomes and therefore challenge organisms to adapt. Lithuanian clean-up workers of the Chernobyl nuclear disaster (LCWC) experienced high doses of IR, leading to different consequences. This study aims to characterise a unique protective genomic variation in a relatively healthy LCWC group. This variation influenced their individual reaction to IR and potentially protects against certain diseases such as exfoliation syndrome and glaucoma. Clinical and IR dosage data were collected using a questionnaire to characterise the cohort of 93 LCWC. Genome-wide genotyping using Illumina beadchip technology was performed. The control group included 466 unrelated, self-reported healthy individuals of Lithuanian descent. Genotypes were filtered out from the microarray dataset using a catalogue of SNPs. The data were used to perform association, linkage disequilibrium, and epistasis analysis. Phenotype data analysis showed the distribution of the most common disease groups among the LCWC. A genomic variant of statistical significance (Fishers’ exact test, p = 0.019), rs3825942, was identified in LOXL1 (NM_005576.4:c.458G>A). Linkage disequilibrium and epistasis analysis for this variant identified the genes LHFPL3, GALNT6, PIH1D1, ANKS1B, and METRNL as potentially involved in the etiopathogenesis of exfoliation syndrome and glaucoma, which were not previously associated with the disease. The LOXL1 variant is mostly considered a risk factor in the development of exfoliation syndrome and glaucoma. The influence of recent positive selection, the phenomenon of allele-flipping, and the fact that only individuals with the homozygous reference allele have glaucoma in the cohort of the LCWC suggest otherwise. The identification of rs3825942 and other potentially protective genomic variants may be useful for further analysis of the genetic architecture and etiopathogenetic mechanisms of other multifactorial diseases.

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Associations of SNP rs_7927894 of FLG gene and TARC/CCL17 with atopic dermatitis in children

Modern pediatrics. Ukraine ◽

10.15574/sp.2021.118.12 ◽

2021 ◽

pp. 12-18

Author(s):

V.O. Dityatkovsky ◽

◽

O.E. Abaturov ◽

N.V. Naumenko ◽

O.O. Alifirenko ◽

...

Keyword(s):

Atopic Dermatitis ◽

Statistical Significance ◽

Venous Blood ◽

Clinical Signs ◽

Main Group ◽

Control Group ◽

Serum Concentrations ◽

Control Groups ◽

And Control ◽

Severity Degree

One of the main genetic factors of the development of atopic dermatitis (AD) in children are single nucleotide polymorphisms (SNP) of the filagrin gene (FLG), particularly rs_7927894 FLG. One of the mostly studied and promising AD marker chemokines (CK) is the thymusE and activation regulated chemokine (TARC/CCL17). Purpose – to detect the associations and role of different variants of SNP rs_7927894 FLG gene and TARC/CCL17 in children suffering different AD clinical proE files (CP) – isolated or combined with comorbid atopic disorders (AtD). Materials and methods. The main group comprised 39 patients aged 3 to 18 years, suffering the isolated AD or combined with comorbid AtD. The control group comprised 47 patients aged 3 to 18 years, suffering the pathology of gastrointestinal tract without clinical signs of atopy. All the patients of the main and control groups had undergone detection of the genotype variants of SNP rs_7927894 FLG gene by real-time polymerase chain reaction and detection of TARC/CCL17 serum concentrations in venous blood. The cutEoff value of statistical significance was set as p<0.05. Results. The incidence and association of genotype variants C/C, C/T and T/T SNP rs_7927894 FLG gene in patients of cohorts of the studied groups were detected as follows: C/T rs_7927894 FLG was significantly the most common in the general main group (56.4%, p<0.05), within the cohort of CP AD isolated (61.1%, p<0.05) and CP of AD combined with comorbid AtD (52.4%, p<0.05). There were detected the associations of studied SNP with AD: C/T rs_7927894 FLG is significantly directly associated with AD (r=0.291, p<0.05), C/C rs_7927894 FLG has a reverse association with a trend to significance (r=-0.194, p=0.07). Mean serum concentrations of TARC/CCL17 did not differ significantly among patients cohorts of the main and control groups, respectively: general main group — 615.8 pg/ml, main with a CP AD isolated — 651.3 pg/ml, main with a CP of AD combined with comorbid AtD — 585.4 pg/ml, control — 608.4 pg/ml (p>0.05). Associations of serum TARC/CCL17 concentrations were determined as follows: elevation trending to significance within increasing AD severity degree (r=0.290, p=0.07) and significant elevation within the AD exhacerbation period (r=0.426, p<0.05). No significant association of TARC/CCL17 as to AD patients compared to the control group was detected in our study (r=-0.027, p>0.05). Conclusions. The genotype heterozygote variant C/T rs_7927894 FLG is significantly the most common and associated with all AD CP in children — isolated and combined with comorbid AtD. Variant C/C rs_7927894 of FLG gene is significantly reversely associated with AD in children. Serum concentrations of TARC/CCL17 did not reveal any significant differences between the AD patients and nonEatopic ones. However, they significantly elevate within AD exacerbation phase and trending to significance within AD severity degree increase in children. The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local Ethics Committee of all participating institutions. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the authors. Key words: atopic dermatitis, children, associations, polymorphism, filaggrin, thymus- and activation regulated chemokine.

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