UNUSUALLY LIPID PRODUCTION BY THE MARINE-DERIVED FUNGI Penicillium restrictum

2021 ◽  
Vol 66 (3) ◽  
pp. 91-98
Author(s):  
Tuyen Le Van ◽  
Samuel Bertrand ◽  
Linh Nguyen Ngoc ◽  
Diep Tran Van ◽  
Hoan Phung Van ◽  
...  
Keyword(s):  
Lipid Production ◽  
Industrial Applications ◽  
Chromatography Analysis ◽  
Health And Nutrition ◽  
Carcinoma Cell Lines ◽  
Gas Chromatography Analysis ◽  
Penicillium Restrictum ◽  
High Production ◽  
Epidermal Carcinoma ◽  
Promising Source

Using marine-derived fungi as a source of lipid production holds promise as an alternative to industrial lipid production for health and nutrition in the future. In the present study, this strain showed a high production of lipid, about plus 80% of the total lipids in the host-derived medium. Gas chromatography analysis of fungal lipids revealed the presence of saturated (mainly palmitic acid C16: 0 and stearic acid C18: 0) and unsaturated fatty acid (mainly linoleic acid C18: 2, oleic acid C18: 1). These findings suggest this marine-derived fungus is a promising source for lipid production in various industrial applications. In particular, a fraction containing glycolipids of the crude extract exhibited potential cytotoxic activity on human oral epidermal carcinoma cell lines. This result is very interesting to further isolate and determine the molecular structure of bioactive glycolipids.

Diversity of Root Bacteria from Peanut Cropping Systems

2004 ◽  
Vol 31 (2) ◽  
pp. 86-91 ◽  
Author(s):  
D. T. Gooden ◽  
H. D. Skipper ◽  
J. H. Kim ◽  
K. Xiong
Keyword(s):  
Fatty Acids ◽  
Biological Control ◽  
Gas Chromatography ◽  
Cropping Systems ◽  
Cropping System ◽  
Critical Research ◽  
Chromatography Analysis ◽  
Rotation System ◽  
Gas Chromatography Analysis ◽  
Peanut Production

Abstract Rhizobacteria play an important role in sustainable agriculture via plant growth and biological control of pests in a number of ecosystems. Understanding the interactions of crop rotation and rhizobacteria on peanut production is a critical research need. Development of a database on the rhizobacteria obtained from continuous and rotational fields of peanut was initiated in 1997 and terminated in 2000. Peanut was planted in monoculture for 4 yr. In rotational plots, peanut, cotton, corn, and peanut were planted in sequence. Rhizobacteria were isolated from the roots of crop plants grown in a Norfolk soil near Florence, SC. These isolates were identified by composition of fatty acids from gas chromatography analysis (GC/FAME). Arthrobacter and Bacillus were the major genera from non-rhizosphere soils. At initiation of this study in July 1997, the plots selected for continuous peanut had more diversity in rhizobacteria than those plots selected for rotation. In July 2000, rhizobacteria diversity was greater from peanut roots in the rotation cropping system than continuous peanut. Even though rhizobacteria diversity was greater in the rotation system, higher peanut yields were recorded in the continuous peanut system in 2000. Burkholderia spp. were always isolated from the peanut and other crop rhizospheres at each sampling date.

scholarly journals Musa balbisiana and Musa paradisiaca Starches Increase SCFA and Caspase-3 as well as Decrease β-glucuronidase and MDA of Mouse Model for Colon Cancer

2021 ◽  
Vol 13 (1) ◽  
pp. 91-6
Author(s):  
Diana Nur Afifah ◽  
Fauzia Purnamasari ◽  
Luthfiatul Khusna ◽  
Noviasti Rahma Utami ◽  
Aida Fitri Nazillah ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Mouse Model ◽  
Resistant Starch ◽  
Caspase 3 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Musa Balbisiana ◽  
Chromatography Analysis ◽  
Musa Paradisiaca ◽  
Gas Chromatography Analysis ◽  
Low Glycemic Index

BACKGROUND: Administration of resistant starch (RS) influences the diversity and the composition of microbiota as well as inhibits the growth of cancer cell. Banana as a potential source of RS has been reported. Although Musa paradisiaca has been reported to induce apoptosis in colon cancer cells, Musa balbisiana, which has low glycemic index and suitable for particular patients, has not been investigated yet.METHODS: Starches of M. balbisiana and M. paradisiaca were prepared and mixed with other components to make 3 types of mouse pellets. Mouse model for colon cancer was prepared and fed with different types of mouse pellets. Blood was collected and processed for measuring β-glucuronidase and malondialdehyde (MDA) with Enzyme-linked Immunosorbent Assay (ELISA) method. Resected ceca were incised to collect the inner part for short-chain fatty acid (SCFA) measurement with gas chromatography analysis. Resected colas were fixed and processed for immunohistochemistry to detect Caspase-3.RESULTS: Colon-cancer-mice fed with the M. balbisiana and M. paradisiaca starches-contained pellets had significant higher concentrations of total SCFA (p=0.003), acetic acid (p=0.000), propionic acid (p=0.000) and butyric acid (p=0.000); lower concentration of β-glucuronidase (p<0.001); higher Caspase-3 score (p=0.040); and lower MDA concentration (p<0.001) than colon-cancer-mice fed with standard pellet (control).CONCLUSION: M. balbisiana and M. paradisiaca starches could be suggested as potential anti-colon cancer RS. Further research should be carried out to disclose the starches mechanisms in colon cancer cell.KEYWORDS: Musa balbisiana, Musa paradisiaca, colon cancer, resistant starch, Caspase-3, SCFA, β-glucuronidase, malondialdehyde

scholarly journals Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia

2018 ◽  
Vol 19 (4) ◽  
pp. 1266-1272 ◽  
Author(s):  
MICKY VINCENT ◽  
HUANG CHAI HUNG ◽  
PATRICIA ROWENA MARK BARAN ◽  
AFIZUL SAFWAN AZAHARI ◽  
DAYANG SALWANI AWANG ADENI
Keyword(s):  
Candida Parapsilosis ◽  
Yeast Species ◽  
Lipid Production ◽  
Industrial Applications ◽  
Oleaginous Yeasts ◽  
Intracellular Lipids ◽  
Pichia Manshurica ◽  
Sudan Iv ◽  
Scientific Technical ◽  
Polymerase Chain

Vincent M, Hung MC, Baran PRM, Azahari AS, Adeni DSA. 2018. Isolation, identification and diversity of oleaginous yeastsfrom Kuching, Sarawak, Malaysia. Biodiversitas 19: 1266-1272. The present study was performed to isolate, identify and determine thediversity of oleaginous yeasts from various sources in Kuching, Sarawak (Malaysia). Microscopic observations via light and scanningelectron microscope (SEM) indicated that the yeast isolates were in sizes ranging from 2-3 μm in width and 4-8 μm in length, typical ofmost unicellular ascomycotic fungi. Polymerase Chain Reaction (PCR) and molecular identification performed on the yeast isolates,targeting the D1/D2 region of the 26S rDNA, identified 6 yeast species from the 21 isolates, namely Pichia manshurica (5/21), Candidakrusei (8/21), Candida parapsilosis (1/21), Pichia guilliermondii (2/21), Clavispora lusitaniae (1/21) and Kluyveromyces marxianus(4/21). All 21 yeast isolates accumulated intracellular lipids when grown in nitrogen-limited medium, as tested via Sudan IV staining.The present study is the first to document the production of lipids bodies in C. krusei, C. parapsilosis, and C. lusitaniae. Furtherinvestigations to assess the growth kinetics, lipid production efficiencies and lipids profiles of these oleaginous yeasts may provideinsights into the possible utilization of these isolates for a variety of scientific, technical and industrial applications.

scholarly journals Fermentative Production of Mannosylerythritol Lipids using Sweetwater as Waste Substrate by Pseudozyma antarctica (MTCC 2706)

2021 ◽  
Vol 58 (4) ◽  
pp. 246-258
Author(s):  
Jayata Mawani ◽  
Jagruti Jadhav ◽  
Amit Pratap
Keyword(s):  
Soybean Oil ◽  
Industrial Applications ◽  
Commercial Production ◽  
Liquid Chromatography Mass Spectrometry ◽  
Mannosylerythritol Lipids ◽  
Pseudozyma Antarctica ◽  
Fermentative Production ◽  
Oil Concentration ◽  
High Production ◽  
High Production Cost

Abstract Mannosylerythritol lipids are glycolipid biosurfactants with promising industrial applications. However, their commercial production is hindered due to its high production cost. The current study investigates the use of sweetwater, a by-product of the fat-splitting industry in combination with soybean oil for the production of mannosylerythritol lipids using Pseudozyma antarctica (MTCC 2706). The optimum sweetwater and soybean oil concentration of 22% and 7% (w/v) yielded 7.52 g L–1and 21.5 g L–1 mannosylerythritol lipids at shake flask and fermenter level respectively. The structure and functional groups of mannosylerythritol lipids were confirmed by fourier transform infrared (FTIR) spectroscopy, liquid chromatography-mass spectrometry (LC/MS) and 1H- and 13C-nuclear magnetic resonance (NMR) analysis. Surfactant properties, such as surface tension, critical micelle concentration, foaming and emulsification of mannosylerythritol lipids were also explored.

scholarly journals Anti-arthritic and gastroprotective activities of Ardisia crispa root partially mediated via its antioxidant effect

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
Roslida Abdul Hamid ◽  
Lau Moi Fong ◽  
Yeong Looi Ting
Keyword(s):  
Antioxidant Activities ◽  
Inflammatory Diseases ◽  
Dose Range ◽  
Antioxidant Effect ◽  
Total Phenolic ◽  
Chromatography Analysis ◽  
Gas Chromatography Analysis ◽  
Tnf Α ◽  
Ulcer Model ◽  
Lower Dose Range

AbstractBackgroundThunb A.DC (Myrsinaceae), commonly known as “hen’s eyes”, has been traditionally used in treating various inflammatory diseases. The present study evaluated anti-arthritic, gastroprotective and antioxidant activities ofMethodsAnti-arthritic activity was evaluated in complete Freund adjuvant (CFA)-induced adjuvant arthritis and gastroprotective effect was studied in the ethanol-induced ulcer model in rats. ACRH was further isolated to yield quinone-rich fraction (QRF) and both were analyzed for their total phenolic content, total flavonoid content and antioxidant activities in various antioxidant assays. Both ACRH and QRF were also analyzed for the quinone composition via gas chromatography analysis.ResultsACRH exerted significant reduction of IL-1β and TNF-α at a lower dose range in CFA-induced arthritis, as well as exhibited its cytoprotective effect against ethanol-induced ulcer lesion via involvement of mucosal nonprotein sulfhydryl (NP-SH) groups. ACRH also showed higher phenolic and flavonoid contents, as well as better antioxidant activities than QRF.ConclusionsThese findings demonstrated the plant as a potential anti-inflammatory agent, with ACRH succeeded in inhibiting both arthritic and ulcerogenic effect, possibly mediated via its antioxidant effect.

scholarly journals Smart Method for Carotenoids Characterization in Haematococcus pluvialis Red Phase and Evaluation of Astaxanthin Thermal Stability

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 422
Author(s):  
Patrizia Casella ◽  
Angela Iovine ◽  
Sanjeet Mehariya ◽  
Tiziana Marino ◽  
Dino Musmarra ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Food Industry ◽  
Room Temperature ◽  
Haematococcus Pluvialis ◽  
Storage Period ◽  
Effect Of Temperature ◽  
Official Method ◽  
Chromatography Analysis ◽  
Promising Source ◽  
Degradation Effect

Haematococcus pluvialis microalgae is a promising source of astaxanthin, an excellent antioxidant carotenoid. H. pluvialis, as well as other species, could find more extensive applications as healthy food for a variegated carotenoids composition in addition to astaxanthin. Official method has not currently been used for this purpose. The objective of this work was to propose a method to characterize carotenoids in H. pluvialis after the comparison between spectrophotometric and liquid chromatography analysis. In addition, in order to improve the use of astaxanthin in the food industry, thermal stability was investigated. In this context, the effect of temperature at 40–80 °C, over a 16 h storage period was tested on astaxanthin produced by H. pluvialis. A further test was carried out at room temperature (20 °C) for seven days. A decrease in the astaxanthin concentration was observed at all tested temperatures with a decrease >50% of all-trans isomer at 80 °C after 16 h and an increase of 9-cis and 13-cis isomers. In conclusion, the obtained results showed the importance of evaluating the degradation effect of temperature on astaxanthin used as a food additive for a future greater enhancement of this bioproduct in the food field.

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