scholarly journals Comparative study of sonohysterosalpingography with hysterosalpingography for determination of tubal patency in infertile women

Author(s):  
Renuka Kumari ◽  
Priti Bala Sahay

Background: Ever since the history of mankind, human infertility has been a source of personal misery and social stigma. The aim of this study was to evaluate the tubal factors of infertility, especially the tubal patency by SSG and HSG and study the advantages of both.Methods: The present work was undertaken in the Department of Obstetrics and Gynecology and Department of Radiodiagnosis of Rajendra Institute of Medical Sciences, Ranchi. 60 patients with primary or secondary infertility attending the OPD were selected over a period of 15 months (July 2008 to September 2009). Inclusion criteria: women of age 20-35 years with regular menstrual cycle whose male partners had no faults. Exclusion criteria: women with PID or other proven causes of infertility. All the patients were subjected to both SSG and HSG (interval period being 1 menstrual cycle) and the results compared in terms of tubal occlusion detection rate, detection of pelvic pathologies and complications of both the procedures.Results: Out of 60 cases, 70% (42) were of primary infertility and 30% (18) were of secondary infertility. History of reproductive tract infections was higher in secondary infertility group (44%). SSG appeared to be more sensitive in detection of tubal obstruction (35%) than HSG (30%), but the difference was statistically insignificant (p>0.05). HSG could locate the exact site of tubal obstruction which was not evident in SSG. SSG was more sensitive in detection of associated pelvic pathologies (p = 0.05) like ovarian cyst, endometriosis and fibroid uterus. However, HSG was better in diagnosis of genital tuberculosis. 20% of patients complained of pelvic pain, bleeding, infection or allergic reaction during or after HSG, while only 7% of patients reported the same with SSG. Thus, SSG was superior to HSG in this respect (p = 0.02).Conclusions: SSG, though not a substitute, can be used as an alternative to HSG for assessment of tubal factors of infertility.

1968 ◽  
Vol 19 (6) ◽  
pp. 1059 ◽  
Author(s):  
HE Fels ◽  
HG Neil

Six-year-old ewes, the survivors of two experimental groups that had grazed from May to October through the 5 preceding years on mixed pastures containing an oestrogenic variety (Dwalganup) of subterranean clover ("clover" group) and oat crops ("oats" group), were mated 6 months after their last exposure to oestrogenic pasture and allocated for slaughter to observe the fertilization rate of eggs or the numbers of surviving embryos. In late April the clover and oats groups did not differ significantly in the proportion of eggs fertilized (13/15 eggs v. 11/11) but in May there was a highly significant difference (4/12 v. 14/17). The difference between late April and May in the clover group was significant and was associated with changes in the ratio of oestrous ewes to rams. The ratio of live embryos to eggs shed was much higher in the oats than in the clover group (56173 v. 8/39). After estimating how many of these eggs had been fertilized it was concluded that more fertilized eggs developed successfully in the oats than in the clover group (c. 56/65 v. 8/17). The data suggested that more "clover" embryos died during days 13–19 of pregnancy. Significantly more clover group ewes had obvious reproductive tract infections. These ewes did not show oestrus regularly though they may have ovulated more or less regularly. Other clover group ewes showed oestrus as regularly as oats group ewes. Clover group ewes had many more macroscopic endometrial cysts in their uteri and cervices, but within groups, numbers of cysts were not significantly associated with previous or current reproductive performance.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lijuan Cheng ◽  
Yan Gao ◽  
Qing Xia ◽  
Hui Wang ◽  
Xiuzhen Xie ◽  
...  

Abstract Background This study was undertaken to discover whether the vaginal microbe of women at childbearing age is different among groups defined by urogenital tract infections, childbearing history and menstrual cycle, respectively. Results This was a multiple case-control study of women at childbearing age who were assigned to case or control groups according to their states of urogenital tract infections. The participants were also grouped by childbearing history and menstrual cycle. Vaginal swabs were collected and stored at − 70 °C until assayed. The V3-V4 region of 16S rRNA gene was amplified using PCR and sequenced on the Illumina MiSeq platform. We tested the hypothesis of whether the relative abundance of microbial species in vaginal microbiota was varied with urogenital tract infections, childbearing history and menstrual cycle. The vaginal microbial richness (Alpha diversity measured by PD_whole tree) was decreased in normal women (without reproductive tract infections) than in those with bacterial vaginosis (BV), and decreased in pregnant women than in other groups of non-pregnancy. Similarly, women from groups of normal and in pregnancy had lower beta diversity on measure of unweighted_unifrac distance in comparison to those of infected and non-pregnant. The top 10 genus relative abundance, especially Lactobacillus, which was the most dominant genus with the relative abundance of 71.55% among all samples, did not differ significantly between groups of childbearing history and menstrual cycle analyzed by ANOVA and nonparametric kruskal_wallis. Lactobacillus iners and Lactobacillus helveticus have the most abundance, totally account for 97.92% relative abundance of genus Lactobacillus. We also found that a higher L.helveticus/L.iners ratio is more likely to present in normal women than in the infected and in pregnant than in non-pregnant, although these comparisons lack statistical significance. Conclusions The relative abundance of dominant bacterial taxa in vaginal microbial communities of women at childbearing age were not different among groups of childbearing history and menstrual cycle. Women from groups of in pregnancy and without reproductive tract infections had lower alpha and beta diversity. The composition of the main lactobacillus species may shift upon phases of a menstrual cycle and the status of reproductive tract infections.


Author(s):  
Miti Dwijen Bhatt ◽  
Deepali Mohan Kadam

Background: Being a developing country, India is faced with many health problems which can be averted by education and implementation of basic guidelines. Menstrual hygiene is one such topics which is rarely brought to attention but forms an integral part of a women’s life. This topic has been neglected not only in rural areas but in the poor socioeconomic corners of metropolitan cities. This ignorance has taken a great toll on the quality of life of women. Poor hygiene predisposes them to reproductive tract infections which may lead to increased incidence of infertility and mortality. Misconceptions regarding this have also prevented women to seek out professional help. Keeping this in mind the study was undertaken to spread awareness and eliminate the taboo this topic is regarded with.Methods: A cross sectional study was conducted amongst adolescent girls in a private school located in Angaon village, Bhiwandi Taluka, Mumbai. A self-administered questionnaire was given to the adolescent girls to assess their knowledge, attitude and practices regarding menstrual cycle. Percentages were used for statistical analysis.Results: 83 girls (96.51%) were aware of menstrual cycle. 49 (56.98%) girls perceived that menstrual blood is dirty. 72 (83.72%) knew that it is a physiological process. 85 (98.84%) were also aware about menstrual hygiene. 85 (98.4%) felt that girls should be addressed regarding menstrual cycle, hygiene before achieving menarche. 50 (58.14%) girls followed restrictions during menstruation.Conclusions: The findings of the present study emphasize the need to address physiology about menstrual cycle as well as the myths and misconceptions associated with it.


2021 ◽  
Author(s):  
Yan Gao ◽  
Lijuan Cheng ◽  
Qing Xia ◽  
Hui Wang ◽  
Xiuzhen Xie ◽  
...  

Abstract Background: The association of the normal physiological cycle to the structural pattern of microbiota in reproductive tract of women at reproductive age has not been extensively explored. This study was undertaken to determine whether the vaginal microbes of women at childbearing age is different among groups defined by urogenital tract infections, childbearing history and menstrual cycle, respectively.Results: This was a multiple case-control study of women at childbearing age who were assigned to case or control groups according to their states of urogenital tract infections. The participants were also grouped by childbearing history and menstrual cycle. Samples of vaginal swabs were collected and stored at -70℃ until assayed. The V3-V4 regions of 16S rRNA genes were amplified using PCR and sequenced on the Illumina MiSeq platform. We tested the hypothesis of whether the relative abundance of microbial species in vaginal microbiota was different between women with different urogenital tract infections, childbearing history and menstrual cycle. We showed that the vaginal microbial richness(Alpha diversity measured by PD_whole tree) was decreased in normal women(without reproductive tract infections) than in those with bacterial vaginosis (BV), and decreased in pregnant women than in other groups of non-pregnancy. Similarly, women from groups of normal and in pregnancy had lower beta diversity on measure of unweighted_unifrac distance in comparison to those of uninfected and non-pregnant. The top 10 genus relative abundance, especially that Lactobacillus was the most dominant genus with the relative abundance of 71.55% among all samples, did not differ significantly between groups of childbearing history and menstrual cycle analyzed by ANOVA and nonparametric kruskal_wallis.Lactobacillus iners and Lactobacillus helveticus have the most abundance, totally account for 97.92% relative abundance of genus Lactobacillus. It is proposed that a higher L.helveticus/L.iners ratio is more likely to present in normal women than in the infected and in pregnant than in non-pregnant, although this comparison lacks statistical significance.Conclusions: The relative abundance of dominant bacterial taxa in vaginal microbial communities of women at childbearing age, characterized with 16S rRNA gene sequence and QIIME based analysis, were not different among groups of childbearing history and menstrual cycle. Women from groups of in pregnancy and without reproductive tract infections had lower alpha and beta diversity. The compositional ratio of the main lactobacillus species may shift depending on the normal physiological cycle and reproductive tract infections.


2020 ◽  
Author(s):  
Yan Gao ◽  
Lijuan Cheng ◽  
Qing Xia ◽  
Hui Wang ◽  
Xiuzhen Xie ◽  
...  

Abstract Background: The association of the normal physiological cycle to the structural pattern of microbiota in reproductive tract of women at reproductive age has not been extensively explored. This study was undertaken to determine whether the vaginal microbes of women at childbearing age is different among groups defined by urogenital tract infections, childbearing history and menstrual cycle, respectively.Results: This was a multiple case-control study of women at childbearing age who were assigned to case or control groups according to their states of urogenital tract infections. The participants were also grouped by childbearing history and menstrual cycle. Samples of vaginal swabs were collected and stored at -70℃ until assayed. The V3-V4 regions of 16S rRNA genes were amplified using PCR and sequenced on the Illumina MiSeq platform. We tested the hypothesis of whether the relative abundance of microbial species in vaginal microbiota was different between women with different urogenital tract infections, childbearing history and menstrual cycle. We showed that the vaginal microbial richness(Alpha diversity measured by PD_whole tree) was decreased in normal women(without reproductive tract infections) than in those with bacterial vaginosis (BV), and decreased in pregnant women than in other groups of non-pregnancy. Similarly, women from groups of normal and in pregnancy had lower beta diversity on measure of unweighted_unifrac distance in comparison to those of uninfected and non-pregnant. The top 10 genus relative abundance, especially that Lactobacillus was the most dominant genus with the relative abundance of 71.55% among all samples, did not differ significantly between groups of childbearing history and menstrual cycle analyzed by ANOVA and nonparametric kruskal_wallis. Lactobacillus iners and Lactobacillus helveticus have the most abundance, totally account for 97.92% relative abundance of genus Lactobacillus. It is proposed that a higher L.helveticus/L.iners ratio is more likely to present in normal women than in the infected and in pregnant than in non-pregnant, although this comparison lacks statistical significance.Conclusions: The relative abundance of dominant bacterial taxa in vaginal microbial communities of women at childbearing age, characterized with 16S rRNA gene sequence and QIIME based analysis, were not different among groups of childbearing history and menstrual cycle. Women from groups of in pregnancy and without reproductive tract infections had lower alpha and beta diversity. The compositional ratio of the main lactobacillus species may shift depending on the normal physiological cycle and reproductive tract infections.


Author(s):  
Anjana Verma ◽  
Ashish Patyal ◽  
Medha Mathur ◽  
Navgeet Mathur ◽  
Jitendra Hirani

Background: Indian tribes are culturally distinct communities, with unique traditions and practices. Lack of awareness and “culture of silence” about reproductive health issues among women in rural areas contributes to high burden of reproductive tract infections/sexually tract infections. Rural tribal women also suffer from ignorance, poverty and lack of access to quality health care, which makes them more vulnerable to RTIs/STIs.Methods: It was a community based cross sectional study conducted among 200 tribal women living in rural Udaipur, Rajasthan. The study was conducted from May 2019 to November 2019. A predesigned, pretested and structured questionnaire was used to take the interview of eligible women. Questionnaire included questions about sociodemographic profile, questions to assess the knowledge regarding RTIs and history of occurrence of any RTI symptom in the past six months and; questions to assess the treatment seeking behaviour for RTI symptoms.Results: Most of the participants were 26-35 years old.About 37% gave the history of experiencing RTI symptoms, vaginal discharge being the most common (46%) symptom. Regarding the treatment, most (34%) of the females took treatment from government hospital, followed by 26% of women who bought the medicines direct from pharmacy (over the counter), about 16% consulted a private practitioner. Prevalence was found to be significantly higher in less educated, belonging to lower socioeconomic status and among non-users of contraceptive methods.Conclusions: Health education and active participation of stakeholders in the reproductive health programs is essential to strengthen the ongoing schemes about improving reproductive health of tribal women.


2020 ◽  
Author(s):  
Yan Gao ◽  
Hongyan Liu ◽  
Lijuan Cheng ◽  
Qing Xia ◽  
Xiuzhen Xie ◽  
...  

Abstract Background: The association of the normal physiological cycle to the structural pattern of microbiota in reproductive tract of women at reproductive age has not been extensively explored. This study was undertaken to determine whether the vaginal microbes of women at childbearing age is different among groups defined by urogenital tract infections, childbearing history and menstrual cycle, respectively.Results: This was a multiple case-control study of women at childbearing age who were assigned to case or control groups according to their states of urogenital tract infections. The participants were also grouped by childbearing history and menstrual cycle. Samples of vaginal swabs were collected and stored at -70℃ until assayed. The V3-V4 regions of 16S rRNA genes were amplified using PCR and sequenced on the Illumina MiSeq platform. We tested the hypothesis of whether the relative abundance of microbial species in vaginal microbiota was different between women with different urogenital tract infections, childbearing history and menstrual cycle. We showed that the vaginal microbial richness(Alpha diversity measured by PD_whole tree) was decreased in normal women(without reproductive tract infections) than in those with bacterial vaginosis (BV), and decreased in pregnant women than in other groups of non-pregnancy. Similarly, women from groups of normal and in pregnancy had lower beta diversity on measure of unweighted_unifrac distance in comparison to those of uninfected and non-pregnant. The top 10 genus relative abundance, especially that Lactobacillus was the most dominant genus with the relative abundance of 71.55% among all samples, did not differ significantly between groups of childbearing history and menstrual cycle analyzed by ANOVA and nonparametric kruskal_wallis.Lactobacillus iners and Lactobacillus helveticus have the most abundance, totally account for 97.92% relative abundance of genus Lactobacillus. It is proposed that a higher L.helveticus/L.iners ratio is more likely to present in normal women than in the infected and in pregnant than in non-pregnant, although this comparison lacks statistical significance.Conclusions: The relative abundance of dominant bacterial taxa in vaginal microbial communities of women at childbearing age, characterized with 16S rRNA gene sequence and QIIME based analysis, were not different among groups of childbearing history and menstrual cycle. Women from groups of in pregnancy and without reproductive tract infections had lower alpha and beta diversity. The compositional ratio of the main lactobacillus species may shift depending on the normal physiological cycle and reproductive tract infections.


Dairy ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 40-64
Author(s):  
Eduardo Barahona Rosales ◽  
Burim N. Ametaj

Postpartum uterine diseases are common in dairy cows and are a great concern for the dairy industry as they are associated with various consequences, including lower fertility, lower milk yield, and an overall negative impact on the host health. An infected uterus is a source of bacterial compounds and cytokines that spill into the systemic circulation, spreading inflammation to other organs. In this review article, we discuss a short overview of the anatomy of the reproductive tract of dairy cows and several infectious diseases of the uterus including metritis, endometritis, and pyometra. Additionally, we discuss the microbiome of the reproductive tract in health and during uterine diseases. As well, diagnostic criteria for metritis and endometritis and contributing factors for increased susceptibility to metritis infection are important topics of this review. To better understand how the uterus and reproductive tract respond to bacterial pathogens, a section of this review is dedicated to immunity of the reproductive tract. Both the innate and adaptive immunity systems are also discussed. We conclude the review with a factual discussion about the current treatments of uterine diseases and the new developments in the area of application of probiotics for uterine health. Mechanisms of actions of probiotics are discussed in detail and also some applications to prevent uterine infections in dairy cows are discussed.


Author(s):  
Padmalaya Das ◽  
Danielle Lisnek ◽  
Krushna Chandra Sahoo ◽  
Shalini Sinha ◽  
JyotiRanjan Mohanty ◽  
...  

A large proportion of women in Odisha, India, use reusable absorbents to manage their menstruation. Yet, the risk factors for lower reproductive tract infections (RTIs) related to menstrual hygiene management (MHM) have not been studied among reusable absorbent users. Women of reproductive age attending one of two hospitals from two different cities in Odisha during two separate study intervals were recruited for the study. Laboratory diagnosis of bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC) were conducted. A questionnaire was used to collect information on MHM practices, water, sanitation, and socio-demographic factors. Among the 509 women who used reusable absorbents, 71.7% were diagnosed with at least one infection. After adjusting for confounders, women with BV were more likely to identify as being a housewife (aOR: 1.8 (1.1–2.9)). Frequent absorbent changing was protective against BV (aOR: 0.5 (0.3–0.8)), whereas frequent body washing increased the odds of BV (aOR: 1.5 (1.0–2.2)). Women with VVC were more likely to be older (aOR: 1.6 (1.0–2.5)), live below the poverty line (aOR: 1.5 (1.1–2.2)), have a non-private household latrine (aOR: 2.2 (1.3–4.0)), dry their absorbents inside the house (aOR: 3.7 (2.5–4.5)), and store absorbents in the latrine area (aOR: 2.0 (1.3–2.9)). Washing absorbents outside the house was protective against VVC (aOR: 0.7 (0.4–1.0)). This study highlights the importance of improving MHM practices among reusable absorbent users to prevent lower RTIs among women reusing menstrual materials in Odisha.


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