scholarly journals Experience with NESTROFT for screening for thalassemia trait/ minor: evaluation against CBC and HPLC in a high prevalence region in Saurashtra, Gujarat, India

2020 ◽  
Vol 8 (3) ◽  
pp. 1108
Author(s):  
Varsha P. Sorathiya ◽  
Nishith A. Vachhani ◽  
Sanjeev L. Nandani ◽  
Daya J. Vekariya ◽  
Heena N. Kashiyani ◽  
...  
Keyword(s):  
High Performance ◽  
Complete Blood Count ◽  
Osmotic Fragility ◽  
Cost Effective ◽  
Red Cell ◽  
Simple Method ◽  
High Prevalence ◽  
Prevention Programmes ◽  
Red Cell Indices ◽  
Thalassemia Trait

Background: Hemoglobinopathies pose a significant health burden in India. Prevention programmes can significantly reduce this burden. Although sophisticated methods of screening for β thalassemia trait are available, a cheap and simple method is beneficial for population screening. Although the Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) has been evaluated in many studies, sample sizes were small in some and many earlier studies have not done complete blood count (CBC) and High-Performance Liquid Chromatography (HPLC) in all the cases. We evaluate the suitability of NESTROFT for detection of β-thalassemia trait in a high prevalence region in Saurashtra, Gujarat.Methods: Here, 1000 unrelated individuals were studied. NESTROFT, CBC and estimation of HbA2 and HbF or other hemoglobin variants were done by HPLC.Results: Prevalence of β thalassemia trait was 7.8% in this population. NESTROFT showed an overall sensitivity and specificity of 94.87 and 85.38 respectively for the detection of β thalassaemia trait. Using red cell indices (MCH <27 pg and MCV <80 fl), One β thalassemia trait with normal indices would have been missed. Among twelve individuals with other hemoglobinopathies (HbS, HbD, HbE, δβ thalassemia trait or HPFH), seven had a positive NESTROFT while three had normal MCV & MCH values.Conclusions: NESTROFT is a cost-effective sensitive test which does not require any equipment and can be done in remote areas. It remains a useful first line screening test when large populations have to be screened. 

scholarly journals An analysis of NESTROFT and Red cell indices in evaluating antenatal mothers for Beta Thalassemia trait

2018 ◽  
Vol 17 (3) ◽  
pp. 411-416
Author(s):  
Rana Safia ◽  
Zeeba S Jairajpuri ◽  
Shaan Khetrapal ◽  
MJ Hassan ◽  
Monika Gupta ◽  
...  
Keyword(s):  
Health Care ◽  
Low Cost ◽  
Care Center ◽  
Medical Science ◽  
Osmotic Fragility ◽  
Beta Thalassemia ◽  
Red Cell ◽  
Health Care Center ◽  
Red Cell Indices ◽  
Thalassemia Trait

Background: The frequency of thalassemia trait is about 3% worldwide, while in developing countries like India, it is a major cause of burden on the health care system. Naked eye single tube red cell osmotic fragility test (NESTROFT) used in population screening for beta thalassemia trait. It is a simple, low cost, reliable and most suitable screening test for β BTT, with a sensitivity of 99.8% in regions with high prevalence rates.Material &Methods: The present study was conducted in the Department of Pathology and included a total of 174 antenatal cases, attending the Out Patient Department of Obstetrics and Gynecology of a tertiary health care center in New Delhi between June 2011 to January 2012. The aim of the study was to screen mothers antenatally for early detection of BTT and to test the validity of NESTROFT in detection of thalassemic carriers of this area.Results: 174 pregnant women attending antenatal clinics were screened for detecting hemoglobinopathy with the help of NESTROFT, red cell indices, haemoglobin electrophoresis and HPLC It was seen that only four cases were BTT while ten were of microcytic anaemia and two had normal red cell indices.Conclusion: antenatal diagnosis of BTT is an important screening programme to reduce the burden of birth of children suffering from thalassemia and other hemoglobinopathies.Bangladesh Journal of Medical Science Vol.17(3) 2018 p.411-416

scholarly journals Role of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) in Detecting Beta-Thalassemia Trait

2018 ◽  
Vol 36 (4) ◽  
pp. 145-152 ◽  
Author(s):  
Asma Ferdousi ◽  
Mainuddin Ahmad ◽  
Jhulan Das Sharma ◽  
Rasheda Samad ◽  
AKM Zafar Ullah
Keyword(s):  
Predictive Value ◽  
Disease Incidence ◽  
Osmotic Fragility ◽  
Beta Thalassemia ◽  
Red Cell ◽  
College Hospital ◽  
Single Tube ◽  
Naked Eye ◽  
Thalassemia Trait

Thalassemia is one of the commonest inherited diseases in Bangladesh. The birth of a thalassemic child places considerable strain, not only on the affected child and its family but also on the community and the nation at large. To reduce the burden of the society and to reduce the disease incidence by providing genetic counseling, detection of carrier is important. The present study evaluates the role of ‘Naked Eye Single Tube Red Cell Osmotic Fragility Test’ (NESTROFT) in detecting ß-thalassemia trait. The current study is a cross sectional study done during the period of September 2008 to August 2009. The study subjects were sibs, parents and relatives of thalassemia patients of age more than 1 year attending Pediatric department of Chittagong Medical College Hospital. Sample size was 50. Here subjects with lowered osmotic fragility test were detected and later on Hb- electrophoresis was done. All the data were recorded and analyzed by SPSS programme. The Sensitivity, Specificity and predictive value of positive and negative tests were computed and they were 92.6%, 80%, 92.6% and 80% respectively. False positive cases were found. The present study found NESTROFT to be both sensitive and reasonably specific and of high negative predictive value. However, multicenter study with large sample is needed to recommend NESTROFT as a single screening test for detection of ß-thalassemia trait.J Bangladesh Coll Phys Surg 2018; 36(4): 145-152

α0-Thalassemia Trait with Normal Red Cell Indices: A Report of Two Cases

Hemoglobin ◽  
2012 ◽  
Vol 36 (6) ◽  
pp. 589-591 ◽  
Author(s):  
Can Liao ◽  
Dong-Zhi Li
Keyword(s):  
Red Cell ◽  
Red Cell Indices ◽  
Thalassemia Trait

Red Cell Indices and Formulas Used in Differentiation ofβ-Thalassemia Trait from Iron Deficiency in Thai Adults

Hemoglobin ◽  
2015 ◽  
Vol 39 (4) ◽  
pp. 235-239 ◽  
Author(s):  
Soontharee Plengsuree ◽  
Manoo Punyamung ◽  
Jintana Yanola ◽  
Siriporn Nanta ◽  
Kanokwan Jaiping ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Red Cell ◽  
Red Cell Indices ◽  
Thalassemia Trait

scholarly journals Evaluation of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) in Screening and Diagnosis of Beta Thalassaemia Trait in Pakistani Population

2021 ◽  
Keyword(s):  
Screening Test ◽  
Complete Blood Count ◽  
Osmotic Fragility ◽  
Rapid Screening ◽  
Red Cell ◽  
Pakistani Population ◽  
Single Tube ◽  
Beta Thalassaemia ◽  
Naked Eye ◽  
Thalassaemia Trait

Background: Beta thalassaemia is an inherited disease that is a cause of continuous burden on the affected families and the society. The objective of the study was to evaluate the efficacy of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) for the detection of beta thalassaemia trait when used for the screening in Pakistani population. Methods: All participants requesting a complete blood count were included in the study. Informed consent was obtained. Naked Eye Single Tube Red Cell Osmotic Fragility Test was performed with freshly prepared 0.36% buffered saline. Turbidity in the tubes was an indication for individuals to be positive for beta thalassaemia trait. Hemoglobin electrophoresis was performed on all positive cases to confirm the sensitivity and the reliability of the screening test. Chi-Square test was used to test for any significant correlation between the different hematological parameters and other variables. Results: A total of 812 patients were included in the study from January 2017 to June 2017. The mean age of participants was 35.5 + 13.6 years. There were 351 (43.2%) male and 461 (56.7%) female participants. A 100% Naked Eye Single Tube Red Cell Osmotic Fragility Test sensitivity was obtained with 65 (8%) positive cases having HbA2 levels of more than 3.2% indicating their true positivity whereas, all 747 (92%) negative cases having HbA2 levels of less than 3.2% indicating their true negativity. Conclusion: NESTROFT was found and a rapid screening test for the identification of carriers of beta thalassaemia trait in our population.

SERS-Active Ag Decorated Polymer Nanorod Substrate Fabricated by the Combination of Photochemical Reduction and Nanoimprint Technology

2014 ◽  
Vol 886 ◽  
pp. 101-104 ◽  
Author(s):  
Si Si Liu ◽  
Zhi Mou Xu ◽  
Tang You Sun ◽  
Wen Ning Zhao ◽  
Xing Hui Wu ◽  
...  
Keyword(s):  
Nanoimprint Lithography ◽  
High Performance ◽  
Rhodamine 6G ◽  
Cost Effective ◽  
Nanorod Array ◽  
Raman Signal ◽  
Photochemical Reduction ◽  
Simple Method

We demonstrated a cost-effective and simple method of fabricating Ag-decorated polymer nanorod (ADPN) array by the combination of fabricated by the combination of photochemical reduction and nanoimprint technology. Here, nanoimprint lithography is utilized to fabricate polymer nanorod array as the periodic temple. Subsequently, ADPNs array can be achieved via UV irradiating. The as-synthesized ADPNs array exhibited a remarkable SERS activity and Raman signal reproducibility to rhodamine 6G, a concentration down to 1011 M can be identified. Our results revealed that the ADPN array is a highly desirable candidate as the reliable enhancer for high performance SERS analysis.

Red Cell Indices and Formulas Used in Differentiation of β-Thalassemia Trait from Iron Deficiency in Thai School Children

Hemoglobin ◽  
2014 ◽  
Vol 38 (4) ◽  
pp. 258-261 ◽  
Author(s):  
Sakorn Pornprasert ◽  
Auttapon Panya ◽  
Manoo Punyamung ◽  
Jintana Yanola ◽  
Chatpat Kongpan
Keyword(s):  
Iron Deficiency ◽  
School Children ◽  
Red Cell ◽  
Red Cell Indices ◽  
Thalassemia Trait

scholarly journals Screening to Determine Prevalence of β-Thalassemia and Iron Deficiency Anemia Among Medical Students

BioSight ◽  
2020 ◽  
Vol 1 (2) ◽  
pp. 24-30
Author(s):  
Saddam Kalwar ◽  
Akbar Agha ◽  
Aribah Naz ◽  
Afsheen Arif
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
High Performance ◽  
Blood Count ◽  
Complete Blood Count ◽  
Deficiency Anemia ◽  
Medical College ◽  
Thalassemia Trait

According to the Thalassemia Federation of Pakistan, the mostly inherited disorder in Pakistan is β-thalassemia, which is characterized by a deficient, abnormal, or lack of β-globin chain synthesis and has a prevalence of 6%. The only method of controlling and preventing β-thalassemia is to increase awareness among students. This was an observational study using a random sampling technique. The Dow-Thalassemia awareness program recruited 915 medical students from the Dow Medical College (DMC) and Sindh Medical College (SMC) to voluntarily donate blood samples, which were analyzed by the naked eye single tube red cell osmotic fragility test (NESTROFT) and complete blood count and results were confirmed by high-performance liquid chromatography and analyzed using the NESTROFT. The samples were collected in 2012-2013. A total of 915 samples, out of these 390 samples, 390/915 (42.6%) samples were positive and complete blood count found 282 (72.3%) were positive for iron deficiency anemia. The remaining 108/390 (27.6%) were confirmed by high-performance liquid chromatography. Only 2.4 % subjects were positive for the β-thalassemia trait. Of 915 students, 57.4% of students were healthy, 39.2% had iron deficiency anemia, and 2.4% were carriers of the β-thalassemia trait. The overall prevalence of β-thalassemia was 38/915 (4.1%), which was lower than observed in previous studies. This study also demonstrated the NESTROFT can be used as a primary method of screening out healthy individuals, where approximately 50% require further screening for β-thalassemia

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