scholarly journals A clinical and biochemical study of dengue fever in Kosi region of Bihar, India

2017 ◽  
Vol 4 (4) ◽  
pp. 964
Author(s):  
Rakesh Kumar ◽  
Shanker Suman ◽  
Rajiv Ranjan
Keyword(s):  
Dengue Fever ◽  
Biochemical Study ◽  
Dengue Infection ◽  
Elevated Serum ◽  
Severe Thrombocytopenia ◽  
Medical College ◽  
Orbital Pain ◽  
Ns1 Antigen ◽  
Morbilliform Rash ◽  
Conjunctival Redness

Background: Dengue is a debilitating arthropod-borne viral (arboviral) disease in humans. To improve diagnostics for patients with dengue so that they can receive effective treatments at earliest. In addition, a better understanding of clinical and biochemical study of dengue cases, effective measures can be aimed for the prevention and control of dengue epidemics.Methods: A retrospective clinical and laboratory study was undertaken among admitted patients in the Department of Physiology at Katihar Medical College and Hospital, Katihar, Kosi region of Bihar, India. Forty-two (42) patients admitted over a period of two years, with laboratory results for NS1 antigen and or /IgM positive, for dengue fever, were taken in the study. Clinical features, haematological and biochemical parameters were noted.Results: Out of the 42 patients, 38 patients were male (90.4%) and 4 patients were female (9.6%). Pyrexia was the major symptom (100%) followed by splitting headache (90.40%), myalgia (76.19%), conjunctival redness (50.00%), skin rash (42.80%), abdominal pain (30.95%), hepatosplenomegaly (28.57) ascites (26.19%) and retro orbital pain (19.04%). Severe thrombocytopenia (69.51%), leukopenia (20.19%) and elevated serum SGOT and SGPT (88.54%) were observed.Conclusions: Dengue infection is increasing proportional to increased urbanization and compromised sanitation measures. Fever associated with headache, retro orbital pain, erythematous morbilliform rash, conjunctival redness and itching in palms and soles along with thrombocytopenia, leukopenia, elevated SGPT should prompt a clinician on the possibility of dengue infection.

scholarly journals Assessment of clinical and hematological profile in dengue fever

2020 ◽  
Vol 7 (9) ◽  
pp. 1418
Author(s):  
Mehul K. Patel ◽  
Hitesh J. Patel
Keyword(s):  
Dengue Fever ◽  
Leucocyte Count ◽  
Dengue Infection ◽  
Control Measures ◽  
Total Leukocyte Count ◽  
Total Leucocyte Count ◽  
Severe Thrombocytopenia ◽  
Tropical Regions ◽  
Ns1 Antigen ◽  
Automated Cell Counter

Background: Dengue is one of the most important viral diseases especially in the tropical regions. According to the WHO almost 50 million people get dengue infection annually and WHO estimates almost half of the world’s population lives in countries having endemicity for dengue infection. This study is an attempt to elucidate the positive laboratory profile of serologically diagnosed dengue patients so as to facilitate early diagnosis, treatment, management and vector control measures, to reduce the morbidity and mortality because of this disease.Methods: This study was conducted on 80 indoor patients. Patients presenting to the emergency department, outpatient department (OPD) or pediatric OPD with complaints of fever and clinical features of dengue with positive NS1 antigen test or dengue antibody serology IgM or IgG or both were included in the study. Hemogram was done on automated cell counter analyzer (Sysmex XP 100) which included hemoglobin, hematocrit, total leucocyte count (TLC), differential leucocyte count (DLC) and platelets count.Results: Raised hematocrit (>47%) was noted in 10 (16.6%) of patients at presentation and the hematocrit ranged from 20- 51%. The total leukocyte count ranged from 1500 cells/cumm to >11000 cells/cumm. Leucopenia with less than 4000 cells/cumm was present in 25 % cases.  In the present study out of 80 cases of dengue fever, 85% cases had thrombocytopenia and 15% cases had severe thrombocytopenia (< 20,000/cumm) with bleeding manifestations.Conclusions: Hemoconcentration, leucopenia, thrombocytopenia, and raised liver enzymes SGOT and SGPT along with reactive/ plasmacytoid lymphocytes on peripheral smear gives enough clues to test for dengue serology so that dengue cases can be diagnosed in their initial stages.

scholarly journals NS-1 antigen positive Dengue Infection and molecular characterization of Dengue Viruses in a private Medical College Hospitalin Dhaka, Bangladesh

2018 ◽  
Vol 17 (4) ◽  
pp. 669-673
Author(s):  
Mahmuda Siddiqua ◽  
Ahmed Nawsher Alam ◽  
AKM Muraduzzaman ◽  
Tahmina Shirin
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Medical Science ◽  
Cost Effective ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
Medical College ◽  
Virus Serotype ◽  
Ns1 Antigen

Introduction: Detection of dengue virus infection as soon as possible is critical for management of dengue virus infected patients. Immuno-chromatographic (ICT) tests are easy, cost effective method for dengue virus antigen detection.The sensitivity and specificity of ICT should compare with a gold standard test like RT-PCR. Aim of this study was to compare two test methods (ICT and RT-PCR), observe dengue serotype and seasonal impact on dengue infection.Methodology & result: The patients of Ibn Sina Medical College Hospital from October 2015 to October 2017 were tested for dengue NS1 antigen by ICT method. Out of 3201 sample tested 32.39% were found positive and 89 of which were re-tested for RT-PCR for comparison. Eighty eight of 89 NS1 positive cases showed positive by RT-PCR method giving an accuracy of 98.87%. Among the RT-PCR positive cases 45 were further analyzed for serotype. DEN-1, DEN-2 or both DEN- 1 and DEN-2 were found in 21, 23 and 1cases respectively. No cases of DEN-3 or DEN-4 were detected.Conclusion: This study showed that easily available and cost effective dengue NS1 antigen detection method (ICT) is as effective as molecular test (RT-PCR). DEN-1 and DEN-2 serotype were prevalent during last few years in Bangladesh. Continuous monitoring of dengue virus serotype is important for prevention and control of sudden epidemic by other serotype. Alert to be more during post monsoon when the peak of dengue virus infection was observed.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.669-673

scholarly journals Evaluation of Six Commercial Point-of-Care Tests for Diagnosis of Acute Dengue Infections: the Need for Combining NS1 Antigen and IgM/IgG Antibody Detection To Achieve Acceptable Levels of Accuracy

2011 ◽  
Vol 18 (12) ◽  
pp. 2095-2101 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Richard G. Jarman ◽  
Mark S. Bailey ◽  
Ampai Tanganuchitcharnchai ◽  
Kemajittra Jenjaroen ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Sensitivity And Specificity ◽  
Dengue Infection ◽  
Antibody Test ◽  
Antibody Detection ◽  
Sample Collection ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen ◽  
Antibody Tests

ABSTRACTSix assays were evaluated in this study to determine their suitability for the diagnosis of acute dengue infection using samples from 259 Sri Lankan patients with acute fevers (99 confirmed dengue cases and 160 patients with other confirmed acute febrile illnesses): (i) the Merlin dengue fever IgG & IgM combo device (Merlin), (ii) the Standard Diagnostics Dengue Duo nonstructural 1 (NS1) antigen and IgG/IgM combo device (Standard Diagnostics, South Korea), (iii) the Biosynex Immunoquick dengue fever IgG and IgM (Biosynex, France) assay, (iv) the Bio-Rad NS1 antigen strip (Bio-Rad, France), (v) the Panbio Dengue Duo IgG/IgM Cassette (Inverness, Australia), and (vi) the Panbio dengue NS1 antigen strip (Inverness, Australia). The median number of days of fever prior to admission sample collection was 5 days (interquartile range, 3 to 7 days). Sensitivity and specificity of the NS1 antigen tests ranged from 49 to 59% and from 93 to 99%, respectively, and sensitivity and sensitivity of the IgM antibody test ranged from 71 to 80% and from 46 to 90%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics Dengue Duo test gave the best compromise of sensitivity and specificity (93% and 89%, respectively) and provided the best sensitivity in patients presenting at different times after fever onset. The Merlin IgM/IgG antibody tests correctly classified 64% and 86% of the primary and secondary dengue infection cases, respectively, and the Standard Diagnostics IgM/IgG antibody tests correctly classified 71% and 83% of the primary and secondary dengue infection cases, respectively. This study provides strong evidence of the value of combining dengue antigen- and antibody-based test results in the rapid diagnostic test (RDT) format for the acute diagnosis of dengue.

scholarly journals A clinical and biochemical laboratory profile to measure the severity of dengue fever and their outcome

2020 ◽  
Vol 8 (1) ◽  
pp. 108
Author(s):  
Maddipatla Sushma ◽  
M. V. Nagabhushana ◽  
M. Dharaneedhar Reddy
Keyword(s):  
Dengue Fever ◽  
Dengue Infection ◽  
Elevated Serum ◽  
Dengue Shock Syndrome ◽  
Positive Outcome ◽  
Acute Onset ◽  
Presenting Symptoms ◽  
Elevated Liver Enzymes ◽  
The Common ◽  
Diagnostic Modalities

Background: To identify various manifestations of dengue fever, complications, and to measure their association with laboratory findings.Methods: 100 cases of suspected adults between 20-70 years of age with clinical features suggestive of dengue infection and patients presenting with fever of acute onset (<2 weeks), pain abdomen, vomiting, rash, flushed appearance and bleeding manifestation were studied. All cases were followed up for the clinical and laboratory parameters and treated according to WHO guidelines.Results: 36 were classified as classical dengue fever, 33 as dengue haemorrhagic fever (DHF), 15 as dengue shock syndrome (DSS), and 16 as dengue-like illness (DLI) and the common age group was 30-40 year (50%). Most (66%) of the patients were male. The common presenting symptoms was fever 65%, vomiting (40%), abdominal pain (40%), myalgia (7%), etc. Hepatomegaly (53%), ascites (1%), splenomegaly (8%) was noted. The mean platelet in the present study was 41870 cells/cu mm. Elevated liver enzymes and elevated serum creatinine was found in complicated forms of disease. The prothrombin time ranged from 11-60 sec with a mean of 19.5 sec.Conclusions: The treatment of dengue is mainly supportive, but early institution and meticulous monitoring are the important steps for positive outcome. Much more awareness, vigilance and research in the diagnostic modalities are further needed to avoid unnecessary panic and platelet transfusions.

scholarly journals Clinical profile of dengue fever with severe thrombocytopenia and its complications: a retrospective study at a tertiary care hospital in South India

2017 ◽  
Vol 5 (5) ◽  
pp. 1751 ◽  
Author(s):  
Ch. Manoj Kumar ◽  
K. S. Keerthi Vyas ◽  
Y. Sai Krishna
Keyword(s):  
Retrospective Study ◽  
Dengue Fever ◽  
South India ◽  
Tertiary Care Hospital ◽  
Dengue Infection ◽  
Tertiary Care ◽  
Clinical Profile ◽  
Severe Thrombocytopenia ◽  
Care Hospital ◽  
Haemorrhagic Fever

Background: Dengue haemorrhagic fever is a potentially lethal illness that is universally prevalent in the tropics and has become a major health concern globally in recent decades. The clinical manifestation of dengue infection varies from asymptomatic to severe life threatening illness in the form of DHF/DSS. Dengue haemorrhagic fever or DSS may be fatal in 40% to 50% of untreated patients. A hallmark of dengue infection is severe thrombocytopenia which causes concern for the patients and treating doctors. The objective of this study was to correlate clinical profile during the evolution of dengue fever with severe thrombocytopenia (platelets <10,000/mm3), and comparing frequencies between the different clinical forms in order to predict the severity of the disease.  The present study includes 40 individuals who were found to be seropositive with the detection of NS1Ag, IgM and IgG antibodies for dengue infection with severe thrombocytopenia. Early diagnosis and monitoring is largely dependent on haematological parameters. As no specific antiviral therapy is available, supportive therapy is of utmost importance.Methods: This is an observational, descriptive and retrospective study of 40 patients with clinical and serological diagnosis of dengue fever with severe thrombocytopenia (platelets<10,000/mm3), in the period from August 2015 to September 2016, who were admitted in a tertiary care hospital in South India. ELISA was performed for the detection of dengue NS1, Ig M and Ig G, haematological parameters by automated analyzer and peripheral smear, coagulation profile analysis were done.Results: Out of 40 cases with severe thrombocytopenia, 50% of the patients had classical dengue fever, 30% cases had DHF with bleeding manifests and 20% cases with DHF plasma leakage signs and 5% lead to DSS. There was lack of association studied between severe thrombocytopenia and bleeding manifestations as p value<0.065 was insignificant. However, the risk of complications increased with decreasing platelet counts in the present study.Conclusions: Thrombocytopenia was most predominant haematological discrepancy. There was no predilection for any age group or gender for thrombocytopenia or bleeding among the dengue patients. The results were relevant in assessing the severity of infection and can help by enabling the adaptation of the therapeutic conduct to the needs of individual patients.

scholarly journals Sera-epidemiology of Dengue Virus Infection in Clinically Suspected Patients Attended in Dhaka Medical College Hospital During January to December 2016

2018 ◽  
Vol 26 (2) ◽  
pp. 111-116
Author(s):  
Monira Pervin ◽  
Afroza Akbar Sweety ◽  
Mohammad Zaid Hossain ◽  
Rabeya Sharmin ◽  
Nusrat Fatema ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Clinical Manifestations ◽  
Medical College Hospital ◽  
Dengue Virus Infection ◽  
College Hospital ◽  
Medical College ◽  
Igm Antibody ◽  
Orbital Pain

Introduction: Dengue is now established as one of the most important Arboviral infection in tropical countries. As the epidemic continues worldwide, this Aedes mosquito-transmitted pathogen is considered a major re-emerging tropical disease and significant public health concern. The study was conducted to determine the sero-epidemiology and clinical picture of dengue virus infection among the clinically suspected patients attended in Dhaka Medical College Hospital during January to December 2016.Materials and Methods: The study was carried out on 145 clinically suspected Dengue patients attended in Dhaka Medical College Hospital from January to December 2016 who had fever with temperature >39°C. Blood sample was collected during acute febrile phase of patients and separated sera were tested for NS1 dengue antigen and IgM antibodies using commercial test kits (NS1 by OMC Healthcare (Pvt.) Ltd & IgM antibody by Omega Diagnostics Ltd.).Results: Of the total 145 patients 40 (27.6%) were positive for Dengue NS1 antigen and or IgM antibody test. Of the positive 40 patients, 18 (45%) were positive for NS1, 19 (47.5%) were positive for IgM and 3 (7.5%) were positive for both NS1 and IgM antibody. Major clinical manifestations were fever with arthralgia (79.5%) and retro-orbital pain (50%).Conclusion: Outbreak of Dengue fever is continuing every year and typical presentation of Dengue fever has changed. Arthralgia and retro orbital pain was the most common clinical feature in the present study instead of headache and rash with fever. A large number of suspected dengue cases were negative by Dengue tests for antigen NS1 and IgM antibody which suggests other organisms of similar clinical manifestations are becoming prevalent in Dhaka city.J Dhaka Medical College, Vol. 26, No.2, October, 2017, Page 111-116

scholarly journals Performance evaluation of machine learning-based infectious screening flags on the HORIBA Medical Yumizen H550 Haematology Analyzer for vivax malaria and dengue fever

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Parag Dharap ◽  
Sebastien Raimbault
Keyword(s):  
Machine Learning ◽  
Dengue Fever ◽  
Vivax Malaria ◽  
Dengue Infection ◽  
Reference Method ◽  
Malarial Parasite ◽  
Parasite Life Cycle ◽  
Ns1 Antigen ◽  
Antigen Tests ◽  
Clinical Conditions

Abstract Background Automated detection of malaria and dengue infection has been actively researched for more than two decades. Although many improvements have been achieved, these solutions remain too expensive for most laboratories and clinics in developing countries. The low range HORIBA Medical Haematology Analyzer, Yumizen H550, now provides dedicated flags ‘vivax malaria’ and ‘dengue fever’ in routine blood testing, developed through machine learning methods, to be used as a screening tool for malaria and dengue fever in endemic areas. This study sought to evaluate the effectiveness of these flags under real clinical conditions. Methods A total of 1420 samples were tested using the Yumizen H550 Haematology Analyzer, including 1339 samples from febrile patients among whom 202 were infected with malaria parasites (Plasmodium vivax only: 182, Plasmodium falciparum only: 18, both: 2), 210 were from febrile dengue infected patients, 3 were from afebrile dengue infected patients and 78 were samples from healthy controls, in an outpatient laboratory clinic in Mumbai, India. Microscopic examination was carried out as the confirmatory reference method for detection of malarial parasite, species identification and assessing parasitaemia based on different stages of parasite life cycle. Rapid diagnostic malarial antigen tests were used for additional confirmation. For dengue infection, NS1 antigen detection by ELISA was used as a diagnostic marker. Results For the automated vivax malaria flag, the original manufacturer’s cut off yielded a sensitivity and specificity of 65.2% and 98.9% respectively with the ROC AUC of 0.9. After optimization of cut-off value, flag performance improved to 72% for sensitivity and 97.9% specificity. Additionally it demonstrated a positive correlation with increasing levels of parasitaemia. For the automated dengue fever flag it yielded a ROC AUC of 0.82 with 79.3% sensitivity and 71.5% specificity. Conclusions The results demonstrate a possibility of the effective use of automated infectious flags for screening vivax malaria and dengue infection in a clinical setting.

scholarly journals Serum Ferritin: A Backstage Weapon in Diagnosis of Dengue Fever

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Soumyabrata Roy Chaudhuri ◽  
Subhayan Bhattacharya ◽  
Mainak Chakraborty ◽  
Kingshuk Bhattacharjee
Keyword(s):  
Platelet Count ◽  
Retrospective Study ◽  
Dengue Fever ◽  
Serum Ferritin ◽  
Ferritin Level ◽  
Dengue Infection ◽  
Surrogate Marker ◽  
Febrile Illness ◽  
Dengue Outbreak ◽  
Ns1 Antigen

Aims. This retrospective study evaluates ferritin as a surrogate marker for dengue infection (NS1 and IgM negative stage) as opposed to other febrile illnesses of infective or inflammatory etiology (OFI). Methodology. Data of all patients admitted to medical ward and medical ITU during the dengue outbreak were collected. Patients admitted between 5 and 10 days of febrile illness without a diagnosis were included. Patients with NS1 positivity (Days 2–8) and/or positive IgM for dengue (Days 6–10) were considered to be dengue cases and those with other confirmed diagnoses were considered in the OFI group. Ferritin, CRP, TC of WBC, platelet count, SGOT, SGPT, and albumin levels were analysed for both groups. Results. We examined 30 cases of clinically and serologically confirmed dengue fever and 22 cases of OFI. Ferritin level in dengue cohort was significantly higher than the OFI group (p<0.0001). The best cut-off for ferritin level to differentiate dengue from OFI was found to be 1291. The sensitivity at this cut-off is 82.6% and the specificity at this cut-off is 100%. Conclusion. Ferritin may serve as a significant marker for differentiating between dengue fever and OFI, in absence of a positive NS1 antigen or a positive IgM antibody for dengue.

scholarly journals THE STUDY OF DETECTION OF DENGUE CASES BY NS1 ANTIGEN AND IGM ANTIBODY IN RIMS, ADILABAD, INDIA.

2019 ◽  
Vol 3 (11) ◽  
Author(s):  
Dr. Tanajee Zade ◽  
Dr. K. Srinivas ◽  
Dr. Akshay Berad
Keyword(s):  
Early Diagnosis ◽  
Dengue Fever ◽  
Dengue Infection ◽  
Tertiary Care ◽  
High Morbidity ◽  
Care Hospital ◽  
Serum Samples ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen

Dengue fever is an acute febrile arboviral disease affecting tropical & subtropical regions of the world. Dengue infection produces a spectrum of clinical illness, ranging from an asymptomatic to its most severe form like dengue haemorrhagic fever and dengue shock syndrome. In view of high morbidity and mortality, it is imperative to have a rapid and sensitive laboratory assay for early detection of the dengue infection. The newer parameter NS1 antigen has gained a lot of interest for early diagnosis of the disease. Detection of non-structural antigen (NS1 Ag), IgM and IgG antibody may help in the early diagnosis. The present study was conducted in a RIMS Adilabad, tertiary care hospital & medical college in the Department of  General Medicine. A total of 100 serum samples were processed from suspected cases of dengue fever by using dengue test for detection of NS1 antigen and IgG antibodies. Platelet counts of all these cases were noted. . Of these 100 subjects 85 were serologically proved to have dengue illness, 57 patients were NS1 antigen positive, 28 patients were IgM antibody positive patients. As the NS1 antigen is detectable in blood from day one after onset of fever, its assay is an effective tool for early diagnosis of dengue infection so as to avoid complications. Key words:  Dengue, NS1 Antigen, IgM antibody, Platelet

scholarly journals Clinico-laboratory profile and outcome of dengue fever among children attending a tertiary care hospital of rural Telangana, India

2020 ◽  
Vol 7 (2) ◽  
pp. 382 ◽  
Author(s):  
V. Shekar ◽  
K. Praveen Kumar ◽  
C. Soren ◽  
K. Venkataramana Reddy ◽  
N. Dharani
Keyword(s):  
Dengue Fever ◽  
Tertiary Care Hospital ◽  
Early Recognition ◽  
Dengue Infection ◽  
Tertiary Care ◽  
Warning Signs ◽  
Severe Dengue ◽  
Severe Thrombocytopenia ◽  
Care Hospital ◽  
Presenting Symptoms

Background: Dengue is a self-limiting, vector-borne disease transmitted by Aedes mosquito, causing a major public health threat globally. The objective of this study is to assess the clinical profile and outcome of the dengue infection in children less than 14 years of age September 2018 to August 2019 at the Pediatric Department of S.V.S. Medical College, the tertiary care hospital in Mahabubnagar, Telanagana.Methods: Prospective study of 82 hospitalized children of <14 years with the diagnosis of dengue illness. Children with diagnosis of dengue were classified further in to two groups as per WHO guidelines, Non-severe dengue fever (probable dengue, dengue with warning signs) and ‘Severe Dengue’ (Dengue Haemorrhagic Fever and/or Dengue Shock Syndrome (DHF/DSS). A separate questionnaire form used for documenting clinical history, laboratory parameters. Haematological parameters were noted, chest x-ray, ultra-sonogram in required cases was done. Children were managed as per WHO protocol. The outcomes of the cases were mentioned as discharge, left against medical advice and death.Results: A total of 82 children with dengue were divided in to 55(67%) non severe dengue and 27(33%) severe dengue with males 56(68.2%) and females 26(31.7%). The most common age of presentation was between 6-10 years 34(41.5%). Fever 73(89%) was the most common presenting symptoms. Pleural effusion and hepatomegaly were the commonest clinical findings 28(34.1%) each, which were more among the severe dengue patients. Gall bladder edema 29(35.3%) was the most common ultra-sonogram finding. Significant elevation of transaminases (SGOP, SGPT) was seen in 39(47.5%). Severe thrombocytopenia was observed in 22(26.8%) children. Management was by administration of colloids and crystalloids.Conclusions: Dengue is a global problem. Presenting features include high grade fever, vomiting, abdominal pain, skin rash. Early recognition of symptoms and proper management can reduce the mortality.

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