DEVELOPMENT OF QUICK SCHEME IDENTIFICATION OF LISTERIA USING PHAGE BIOPREPARATION L.M 4 УЛГАУ

2020 ◽  
pp. 191-197
Author(s):  
E.V. Suldina ◽  
Keyword(s):  
Listeria Monocytogenes ◽  
Biological Properties ◽  
Optimal Parameters ◽  
Technological Parameters ◽  
Quick Scheme ◽  
Bacteriological Method ◽  
Culture Production ◽  
Identification Of Bacteria ◽  
And Control ◽  
Minced Meat

The causative agent Listeria monocytogenes causes listeriosis, a severe foodborne illness associated with high mortality. Rapid and sensitive methods are required to detect and identify this pathogen in the food production process. The article presents the results of research on the development of technological parameters for the production of biopreparation based on the bacteriophage L. m 4 UlSAU for quick identification of bacteria of the genus Listeria with its help. It was established that for the production of L. m 4 phage of UlSAU with maximum titers, the optimal parameters are: multiplicity of MOI 1 infection, cultivation temperature-28°C, incubation time of the phage/ culture system – 6 hours. Based on these results, we have offered technological scheme for the production of phage biopreparation L. m 4 UlSAU, which includes the following steps: confirmation of biological properties of indicator phage and increasing its titer (if necessary), verification and confirmation of biological properties of indicator culture, production of phage biopreparation and control of its indicators. Accelerated scheme for Listeria identification was offered with the help of prepared biological product based on L. m 4 bacteriophage of UlSAU. The scheme was tested on samples of chicken meat and minced meat semi finished products artificially contaminated with Listeria monocytogenes bacteria in concentrations 101-105 CFU/ml. It was established that the proposed scheme of listeria identification allows to reduce the duration of studies by 84 hours in comparison with the traditional bacteriological method and to detect listeria at a concentration of 100 CFU/ml (g) in 82 hours. The phagoidentification time at the pure culture typing stage is 18 hours.

DEVELOPMENT OF COMPUTERIZED MONITORING AND CONTROL SYSTEM OF THE MOBILE ROBOT’S POSITIONING ON LARGE FERROMAGNETIC SURFACES BASED ON INTELLIGENT TECHNIQUES. AUTOMATION OF THE MONITORING AND CONTROL PROCESSES OF MOBILE ROBOT FOR PROCESSING OF LARGE INCLINED SURFACES

2019 ◽  
pp. 41-48
Author(s):  
Yan Guojun ◽  
Oleksiy Kozlov ◽  
Oleksandr Gerasin ◽  
Galyna Kondratenko
Keyword(s):  
Control System ◽  
Mobile Robot ◽  
Functional Structure ◽  
Monitoring And Control ◽  
Technological Parameters ◽  
Monitoring And Control System ◽  
Inclined Surfaces ◽  
Computerized Monitoring ◽  
And Control ◽  
Tracked Mobile Robot

The article renders the special features of the design of a tracked mobile robot (MR) for moving over inclined ferromagnetic surfaces while performing specified technological operations. There is conducted a synthesis of the functional structure and selective technological parameters (such as control coordinates) of the computerized monitoring and control system (CMCS) intended for use with this MR. Application of the CMCS with the proposed functional structure allows substantially increasing the accuracy of the MR monitoring and control, which in turn provides for a considerable enhancement in the quality and economic efficiency of the operations on processing of large ferromagnetic surfaces.

Art and an Archaeology of Embodiment: Some Aspects of Archaic Greece

1995 ◽  
Vol 5 (2) ◽  
pp. 207-244 ◽  
Author(s):  
Michael Shanks
Keyword(s):  
Material Culture ◽  
Contemporary Poetry ◽  
Embodied Experience ◽  
Archaic Greece ◽  
Ceramic Vessels ◽  
Culture Production ◽  
City States ◽  
And Control ◽  
Greek World ◽  
Ceramic Decoration

This article seeks to gain an understanding of distinctive changes in certain artefacts produced in Corinth in the late eighth and seventh centuries BC. The focus is the development of figurative imagery on miniature ceramic vessels (many of them perfume jars) which travelled from Corinth particularly to sanctuaries and cemeteries in the wider Greek world. Connections, conceptual and material, are traced through the manufacture and iconography of some 2000 pots, through changing lifestyles, with juxtapositions of contemporary poetry from other parts of the Greek world. Aspects of embodiment are foregrounded in a discussion of stylization and drawing, the character of monstrosity (appearing in ceramic decoration), experiences of risk in battle, discipline and control. Techniques of the self (leading through the floral to wider lifestyles) also feature in this context, together with perfume, and the consumption or deposition of the pots in circumstances of contact with death and divinity. The argument is made that the articulation of an ideological field lay at the core of the changes of the early city states such as Corinth. The article is offered as a contribution to a contextual and interpretive archaeology. It attempts to develop concepts for dealing with power relations in an understanding of material culture production which foregrounds human agency and embodied experience.

scholarly journals Listeria monocytogenes: An emerging food-borne pathogen and its public health implications

2016 ◽  
Vol 10 (02) ◽  
pp. 149-154 ◽  
Author(s):  
Waffa W Reda ◽  
Khaled Abdel-Moein ◽  
Ahmed Hegazi ◽  
Yasmin Mohamed ◽  
Khaled Abdel-Razik
Keyword(s):  
Listeria Monocytogenes ◽  
Raw Milk ◽  
Food Samples ◽  
Food Borne ◽  
Contaminated Food ◽  
Luncheon Meat ◽  
Stool Specimens ◽  
Polymerase Chain ◽  
Minced Meat

Introduction: Listeria monocytogenes is considered one of the most important food-borne pathogens transmitted to humans via contaminated food. The aim of the present study was to demonstrate the importance of L. monocytogenes as a food-borne pathogen. Methodology: A total of 340 samples were collected from different localities in El Giza Governorate, Egypt, to check the occurrence of L. monocytogenes in that area. The collected samples comprised 250 food samples, 40 swabs from food refrigerators, and 50 stool specimens from diarrheic children. L. monocytogenes was isolated from the examined samples according to the International Organization for Standardization. The isolates were tested biochemically using Listeria Microbact 12L and confirmed by polymerase chain reaction. Results: The isolation rates of L. monocytogenes were 8% in beef burger, 4% in minced meat, 4% in luncheon meat, while sausage samples were all negative. Eight percent of raw milk samples were positive for L. monocytogenes, whereas cheese samples and refrigerator swabs were negative. Only Listeria grayi was isolated from human stools (2.5%). Conclusion: The high isolation rates of L. monocytogenes among the examined food stuffs highlight the crucial role of food as an important vehicle for this pathogen. More efforts should be made to ensure safe handling and processing of these foods to reduce the transmission of L. monocytogenes to humans.

BIOLOGICAL CHARACTERISTICS OF BACTERIOPHAGE PHAGUM B.C. 11 UGSHA

2020 ◽  
pp. 174-183
Author(s):  
K. V. Martynova ◽  
Keyword(s):  
Sequence Data ◽  
Raw Materials ◽  
Secondary Growth ◽  
Gc Content ◽  
Bacillus Coagulans ◽  
Passage Time ◽  
Biological Properties ◽  
Lytic Activity ◽  
Bacterial Strains ◽  
Technological Parameters

The article presents the results of research on the biological properties of the bacteriophage Phagum B. c. 11 UGSHA. Bioinformatic sequence data of Bacillus coagulans phage Phagum B. c. 11 UGSHA: length: 42609 bp, GC content: 37,1 %, molecular weight: 27 014 203,97 Da, the molarity of 1 μg/μl: 0.04 μm, the number of molecules in 1 g: 2.23 x 1010, And 260 of 1 μg/mql after 100-fold dilution: 0,259. Experimentally technological parameters of cultivation system phage/host were selected(0.2 ml of bacteriophage to 0.2 ml of the indicator culture B. coagulans), passage time-6 hours at the cultivation temperature- 35±20С. It is recommended to use Millipore membrane filters of 0.22 μm GV for cleaning Phagum B. c. 11 UGSHA. It was determined that the lytic activity by Appelman was 10-9, by Grazia the indicator was4,0+0,1×1010 ((BFU / ml); Phagum B. c. 11 UGSHA had strict specificity in relation to B. coagulans strains; morphology of plaque-forming units (rounded shape with a transparent center, zones of incomplete lysis, diameter 1-4 mm, secondary growth was not observed). It is empirically established that Phagum B. c. 11 UGSHA did not lose its lytic activity after 3 months when stored at a temperature of 2-40С, and after 12 months the indicator decreased to 108. The studied biological properties of bacteriophage Phagum B. c. 11 UGSHA isolated from fresh tomatoes with signs of spoiling, specific for 46 out of 50 bacterial strains of Bacillus coagulans, allow us to recommend Phagum B. c. 11 UGSHA for the production of phage biopreparations used not only in the laboratory for the indication and identification of Bacillus coagulans bacteria specific to it, but also for decontamination of food raw materials and food products, prevention of food poisoning, since the data of our genetic and proteomic mapping allow us to conclude that the Phagum B. c. 11 UGSHA bacteriophage does not contain pathogenicity locuses and their homologues.

Investigation of the Effects of Technological Parameters on Surface Roughness in Extrusion Bars of Aluminum Alloy

2019 ◽  
Vol 889 ◽  
pp. 155-160
Author(s):  
Trong Mai Nguyen ◽  
Đuc Quy Tran ◽  
Van Nghe Pham ◽  
Van Canh Nguyen
Keyword(s):  
Surface Roughness ◽  
Aluminum Alloy ◽  
Surface Quality ◽  
Research Work ◽  
Extrusion Process ◽  
Optimal Parameters ◽  
Technological Parameters

In this research work, the result of the effects of technological parameters on surface roughness in extrusion bars of aluminum alloy were pesented. The results of this study may be used for choosing optimal parameters of extrusion process so that surface quality of extruded bar was improved.

scholarly journals IN VITRO ANTHELMINTIC AND ANTI-AMYLASE PROPERTIES OF GARCINIA PEDUNCULATA ROXB. ETHANOLIC EXTRACT

2016 ◽  
Vol 9 (1) ◽  
pp. 189
Author(s):  
Pinky Sarmah ◽  
Nako Kobing ◽  
Jyotchna Gogoi ◽  
Ananta Madhab Dutta
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Biological Properties ◽  
Death Time ◽  
Control Sets ◽  
Point Analysis ◽  
Soxhlet Apparatus ◽  
And Control

<p><strong>Objective</strong>:<strong> </strong>The present study aims to investigate <em>in vitro</em> anthelmintic and anti-amylase properties of the ethanolic fruit extract of <em>Garcinia pedunculata</em>.</p><p><strong>Methods</strong>:<strong> </strong>For the study, mature fruits of <em>G. pedunculata</em> were collected from local markets during April-May. Fleshy pericarps of fruits were chopped into small pieces, dried and extracted by using a Soxhlet apparatus. Ethanol extract of <em>G. pedunculata</em> was used for evaluation of <em>in vitro</em> anthelmintic and anti-amylase activities. <em>In vitro</em> anthelmintic activity was evaluated in animal models, <em>Pheretima posthuma</em>, an earthworm species. <em>In vitro</em> anti-amylase activity was evaluated by using zymographic, achromic point analysis (Starch-Iodine method) and spectrophotometric method [Di Nitro Salicylic acid (DNS)–Maltose method].</p><p><strong>Results</strong>:<strong> </strong>Ethanolic extract of <em>G. pedunculata</em> showed anthelmintic activity at a concentration of 75 mg/ml, paralysis and death timing was reported at 0.62±0.26 min and 1.42±0.07 min, respectively. The reference standard (Albendazole) showed paralysis time: 2.13±0.28 min and death time: 5.12±0.29 min. In the anti-amylase study, a zymographic density analysis of <em>G. pedunculata</em> showed significant variation in band intensity as compared to Starch–Iodine achromic point analysis and DNS–Maltose method. A concentration of 1.5 mg/ml of extract showed inhibition of amylase: 67.65±1.53 % as compared to other concentrations and control sets.</p><strong>Conclusion</strong>:<strong> </strong>It could be concluded that ethanolic extract of <em>G. pedunculata</em>has biological properties which could be utilised in medicine by characterising its bioactive components.<p> </p>

scholarly journals Effects of Animal, Climatic, Hunting and Handling Conditions on the Hygienic Characteristics of Hunted Roe Doer (Caprelous capreolus L.)

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1076
Author(s):  
Raffaella Branciari ◽  
Andrea Onofri ◽  
Fausto Cambiotti ◽  
David Ranucci
Keyword(s):  
Listeria Monocytogenes ◽  
Roe Deer ◽  
Control Strategies ◽  
Capreolus Capreolus ◽  
Careful Consideration ◽  
Salmonella Spp ◽  
Climate Conditions ◽  
Factors Affecting ◽  
And Control ◽  
Carcass Contamination

The population of wild animals is increasing, and control strategies based on selective hunting are among the major options adopted. The game meat obtained is therefore available for controlled and certified valuable chains. The understanding of carcass contamination and the factors affecting it is therefore crucial to ensure meat safety and prolonged shelf-life. The carcass hygiene of 64 hunted wild male roe deer (Capreolus capreolus L.) was evaluated in relation to factors potentially affecting it. Aerobic colony and Enterobacteriaceae counts, as well as Salmonella spp. and Listeria monocytogenes detection, were performed. The interaction of the microbial determination with age and weight of the animals, the climate conditions, the shooting procedure, the time between the killing and the evisceration as well as the time of storage of the carcasses in refrigerated conditions before skinning, were evaluated. Neither Salmonella spp. nor Listeria monocytogenes were detected on the carcasses and the average loads detected were 3.39 ± 1.06 UFC/cm2 and 2.27± 1.11 UFC/cm2 for the aerobic colony count and Enterobacteriaceae count, respectively. The loads detected are similar to those reported by UE legislation for slaughtered species. The time of storage before skinning, the environmental temperature during hunting and the time between shooting and evisceration, associated with animal weight, affect the carcass hygiene and must be taken into careful consideration by hunters as food business operators.

scholarly journals Alkaloids from Plants with Antimalarial Activity: A Review of Recent Studies

2020 ◽  
Vol 2020 ◽  
pp. 1-17 ◽  
Author(s):  
Philip F. Uzor
Keyword(s):  
Structural Modification ◽  
Antimalarial Activity ◽  
Biological Properties ◽  
Isoquinoline Alkaloids ◽  
Major Health ◽  
Natural Sources ◽  
Financial Status ◽  
Plasmodium Parasite ◽  
Global Issue ◽  
And Control

Malaria is one of the major health problems in developing countries. The disease kills a large number of people every year and also affects financial status of many countries. Resistance of the plasmodium parasite, the causative agent, to the existing drugs, including chloroquine, mefloquine, and artemisinin based combination therapy (ACT), is a serious global issue in malaria treatment and control. This warrants an urgent quest for novel compounds, particularly from natural sources such as medicinal plants. Alkaloids have over the years been recognized as important phytoconstituents with interesting biological properties. In fact, the first successful antimalarial drug was quinine, an alkaloid, which was extracted from Cinchona tree. In the present review work, the alkaloids isolated and reported recently (2013 till 2019) to possess antimalarial activity are presented. Several classes of alkaloids, including terpenoidal, indole, bisindole, quinolone, and isoquinoline alkaloids, were identified with a promising antimalarial activity. It is hoped that the reports of the review work will spur further research into the structural modification and/or development of the interesting compounds as novel antimalarial drugs.

scholarly journals Efficacy of Synthetic Furanones on Listeria monocytogenes Biofilm Formation

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 647 ◽  
Author(s):  
Pedro Rodríguez-López ◽  
Andrea Emparanza Barrenengoa ◽  
Sergio Pascual-Sáez ◽  
Marta López Cabo
Keyword(s):  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Epifluorescence Microscopy ◽  
Dependent Manner ◽  
Antifouling Activity ◽  
Aisi 316 ◽  
Novel Strategy ◽  
And Control ◽  
Acylated Homoserine Lactones ◽  
Dose Dependent

Furanones are analogues of acylated homoserine lactones with proven antifouling activity in both Gram-positive and Gram-negative bacteria though the interference of various quorum sensing pathways. In an attempt to find new strategies to prevent and control Listeria monocytogenes biofilm formation on stainless steel (SS) surfaces, different concentrations of six synthetic furanones were applied on biofilms formed by strains isolated from food, environmental, and clinical sources grown onto AISI 316 SS coupons. Among the furanones tested, (Z-)-4-Bromo-5-(bromomethylene)-2(5H)-furanone and 3,4-Dichloro-2(5H)-furanone significantly (p < 0.05) reduced the adhesion capacity (>1 log CFU cm−2) in 24 h treated biofilms. Moreover, individually conducted experiments demonstrated that (Z-)-4-Bromo-5-(bromomethylene)-2(5H)-furanone was able to not only significantly (p < 0.05) prevent L. monocytogenes adhesion but also to reduce the growth rate of planktonic cells up to 48 h in a dose-dependent manner. LIVE/DEAD staining followed by epifluorescence microscopy visualisation confirmed these results show an alteration of the structure of the biofilm in furanone-treated samples. Additionally, it was demonstrated that 20 µmol L−1 of 3,4-Dichloro-2(5H)-furanone dosed at 0, 24 and 96 h was able to maintain a lower level of adhered cells (>1 log CFU cm−2; p < 0.05). Since furanones do not pose a selective pressure on bacteria, these results represent an appealing novel strategy for the prevention of L. monocytogenes biofilm grown onto SS.

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