scholarly journals Antioxidant capacity of crude extracts containing carotenoids from the berries of various cultivars of Sea buckthorn (Hippophae rhamnoides L.).

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Michał Kruczek ◽  
Adam Swiderski ◽  
Aleksandra Mech-Nowak ◽  
Katarzyna Król

Comparative analysis of antioxidant capacity was performed using FRAP and DPPH methods on extracts containing carotenoids acquired from fruits of Sea buckthorn. The examination included nine varieties of Sea buckthorn growing in the comparative cultivation. Conducted analysis allowed to compare the antioxidant capacity with carotenoids content measured with spectrophotometric and HPLC methods. Three of the examined cultivars indicating high antioxidant activity in both, FRAP and DPPH methods, also revealed highest ('Aromatnaya') and high ('Botanicheskaya', 'Arumnyj') total carotenoids content in HPLC analysis.

Author(s):  
Chagnaadorj Rentsendavaa ◽  
Dóra Székély ◽  
Diána Furulyás ◽  
György Végvári ◽  
Faraja Gonelimali ◽  
...  

Sea Buckthorn (SB) juice is rich in biologically active compounds. It has considerable health benefits; thus, it can serve as functional food ingredient. The aim of this study was to determine and compare the total content of polyphenols, antioxidant capacity (FRAP), soluble solid content, pH value, β-carotene content and colour parameters (L*, a*, b*) in berry juice of cultivar 'Leikora' Sea Buckthorn (SB) (Hippophae rhamnoides L.). The treatment was made as control sample of Sea Buckthorn juice (C), and Sea Buckthorn juice with 0.5 % (P0.5); 1 % (P1); 2 % (P2) dried pomace of Sea Buckthorn. The samples were stored at room temperature for physicochemical analysis at interval of 2 months for a total period of 14 months.According to our results, the β-carotene (C to P2 was 3.71, 4.82, 5.49 and 6.52 mg 100mL−1) as well as the antioxidant capacity of the samples increased with the growth of the pomace content. During storage, degradation occurred in the polyphenol content and antioxidant capacity, but the β-carotene content increased. The increase of the β-carotene content was 94.6 % (C) and 32.7 % (P0.5). The smaller reduction in antioxidant compounds was the higher the sample of pomace content is. The FRAP and total polyphenol values measured during storage confirm that the pomace has antioxidant effect. There is a close correlation between the two parameters, including a positive correlation (r = 0.8614), which indicates that a significant part of the antioxidant capacity of buckthorn is due to the presence of different polyphenols.


Author(s):  
N. Makovics-Zsohár ◽  
A. Hegedűs ◽  
É. Stefanovits-Bányai ◽  
R. Rédei ◽  
N. Papp

Berries of sea buckthorn (Hippophae rhamnoides L.) are characterized by increasing popularity due to their presumable healtheffects. The aim of this study was to compare the antioxidant capacity and total polyphenolic content in the berries of six Hungarian grown sea buckthorn genotypes and characterize the genetic variability in this trait. The harvest time of sea buckthorn berries affects the antioxidant capacity and total phenolic contents in berries of three popular cultivars of German origin. Berries harvested in October had higher antioxidant capacity compared with those harvested one month later. The extent of the difference was genotype-specific. Our analysis revealed a nearly 3-fold difference between the lowest and highest antioxidant capacities of the 6 tested genotypes with ‘Leikora’ showing the highest ferric reducing antioxidant power and total phenolic content. The TEAC values ranged between 1.76 and 3.13 mmol Trolox/100g fresh weight with Pető 1 and ‘Frugana’ having the highest values. The results presented in this study demonstrated that Hippophae rhamnoides berries possess in vitro antioxidant activity strongly determined by genotype but also influenced by harvest time.


Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1170 ◽  
Author(s):  
Adriana Criste ◽  
Adriana Cristina Urcan ◽  
Andrea Bunea ◽  
Flavia Roxana Pripon Furtuna ◽  
Neli Kinga Olah ◽  
...  

Hippophae rhamnoides L. is an important source of natural antioxidant and antimicrobial agents. Phytochemical compounds, antioxidant and antibacterial properties of berries, and leaf extracts from four Romanian sea buckthorn cultivars were investigated. Large differences in the content of total polyphenols and flavonoids between the varieties were observed. HPLC analysis of the polyphenolic compounds showed greater differences in content in leaves than in berries. This study confirmed that sea buckthorn leaves and berries are a rich source of phenolic compounds, especially quercetin derivatives and hydrocinnamic acid derivatives. Five carotenoid compounds were identified in the berries: lutein, zeaxanthin, β-cryptoxanthin, cis-β-carotene, and β-carotene. From the results obtained in this study, it can be stated that the varieties whose berries yielded the highest quantities of polyphenols, flavonoids, and antioxidant activity, can be ranked as follows: SF6 > Golden Abundant > Carmen > Colosal, and for leaf extracts the ranked order is SF6 > Golden Abundant > Colosal > Carmen. A strong correlation between the total flavonoid yield and antioxidant activity (r = 0.96), was observed. All extracts showed antibacterial activity against S. aureus, B. cereus, and P. aeruginosa, however extracts from berries were less potent than extracts from leaves.


2014 ◽  
Vol 23 (3) ◽  
pp. 207-219 ◽  
Author(s):  
Anne Morgenstern ◽  
Anders Ekholm ◽  
Petra Scheewe ◽  
Kimmo Rumpunen

Content of total phenolic compounds and antioxidant capacity (FRAP) were investigated in the leaves of three sea buckthorn (Hippophaë rhamnoides L.) cultivars: ‘Otradnaja’, ‘Gibrid Pertjika’ and ‘Ljubitelskaja’, at different dates. In addition, major phenolic compounds (catechin, kaempferol, quercetin, epigallocatechin, kaempferol-3-O-glucoside, quercetin-3-O-galactoside, isorhamnetin-3-O-glucoside, rutin, gallic acid, procyanidin monomer glycoside, procyanidin dimer aglycone and hydrolyzable tannins I–III) were determined in ‘Ljubitelskaja’. Antioxidant capacity and the content of total phenolic compounds fluctuated during April, May and June, and then increased until the end of July, when the highest values were observed. Total phenolic compounds were strongly correlated with FRAP. Levels were generally higher in ‘Ljubitelskaja’ than in ‘Otradnaja’ and ‘Gibrid Pertjika’. In ‘Ljubitelskaja’, hydrolyzable tannins I–III occurred in higher amounts than did any of the other studied phenolic compounds. The developmental stage of the leaves (harvesting date) had a strong influence on content of phenolic compounds and should be carefully considered when harvesting sea buckthorn leaves for different purposes.


Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 847
Author(s):  
Anita Zapałowska ◽  
Natalia Matłok ◽  
Miłosz Zardzewiały ◽  
Tomasz Piechowiak ◽  
Maciej Balawejder

The aim of this research was to show the effect of the ozonation process on the quality of sea buckthorn (Hippophae rhamnoides L.). The quality of the ozonated berries of sea buckthorn was assessed. Prior to and after the ozone treatment, a number of parameters, including the mechanical properties, moisture content, microbial load, content of bioactive compounds, and composition of volatile compounds, were determined. The influence of the ozonation process on the composition of volatile compounds and mechanical properties was demonstrated. The ozonation had negligible impact on the weight and moisture of the samples immediately following the treatment. Significant differences in water content were recorded after 7 days of storage. It was shown that the highest dose of ozone (concentration and process time) amounting to 100 ppm for 30 min significantly reduced the water loss. The microbiological analyses showed the effect of ozone on the total count of aerobic bacteria, yeast, and mold. The applied process conditions resulted in the reduction of the number of aerobic bacteria colonies by 3 log cfu g−1 compared to the control (non-ozonated) sample, whereas the number of yeast and mold colonies decreased by 1 log cfu g−1 after the application of 100 ppm ozone gas for 30 min. As a consequence, ozone treatment enhanced the plant quality and extended plant’s storage life.


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