scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI ASCIDIAN (Lissoclinum badium) DARI PERAIRAN PULAU MANTEHAGE

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 897
Author(s):  
Falinry Woran ◽  
Defny S. Wewengkang ◽  
Meilani Jayanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Methanol Fraction ◽  
Agar Method ◽  
Ethanol Extracts ◽  
Escherichia Coli Bacteria ◽  
And Staphylococcus Aureus ◽  
Growth Of Bacteria

ABSTRACTLissoclinum badium is a type of ascidian that contains bioactive compounds. This study aims to determine of presence of antibacterial activity from extracts and fractions of Lissoclinum badium collected from Mantehage Island Manado against Escherichia coli and Staphylococcus aureus bacteria. Samples were extracted by maceration method using 95% ethanol solvent and fractionated using solvents of chloroform, n-hexane and methanol. Antibacterial activity was carried out by the disk diffusion agar method. The results showed that the ethanol extracts an methanol fraction had activity to inhibit the growth of Escherichia coli bacteria with strong category. Meanwhile, against the Staphylococcus aureus the ethanol extracts, chloroform and n-hexane fractions had ability to inhibit the growth of bacteria with weak category.. Keywords: Antibacterial activity, Lissoclinum badium, Escherichia coli, Staphylococcus aureus.  ABSTRAKLissoclinum badium merupakan salah satu jenis tunikata yang memiliki kandungan senyawa bioaktif yang dapat digunakan untuk pengobatan berbagai penyakit. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari ekstrak dan fraksi  Lissoclinum badium yang diperoleh dari Pulau Mantehage Manado terhadap Escherichia coli dan Staphylococcus aureus. Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol 95% dan fraksinasi menggunakan pelarut metanol, kloroform, dan n-heksan. Aktivitas antibakteri dilakukan dengan  metode difusi agar cakram kertas. Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi metanol memiliki aktivitas untuk menghambat bakteri Escherichia coli dan Staphylococcus aureus dengan daya hambat kuat. Sedangkan untuk fraksi kloroform dan fraksi n-heksan memiliki aktivitas untuk menghambat bakteri Staphylococcus aureus saja dengan daya hambat sedang. Kata kunci: Aktivitas antibakteri, Lissoclinum badium, Escherichia coli, Staphylococcus aureus.

scholarly journals The Antibacterial Activity Of Several Sponges From The Waters Of Tasik Ria Against Escherichia coli And Staphylococcus aureus bacteria

2018 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Abrianto A. O. Rompis ◽  
Fitje Losung ◽  
Deiske A. Sumilat ◽  
Agung B. Windarto ◽  
Stenly Wullur ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Natural Compounds ◽  
E Coli ◽  
Escherichia Coli Bacteria ◽  
And Staphylococcus Aureus ◽  
Positive Results ◽  
Sponge Extract

The sponge is one of the sea organisms that has a prospect as a source of natural compounds including peptides, steroids, asetogenin, terpenoids, alkaloids, cyclic halide and nitrogen. This research was directed to obtain several species of sponges from the waters of Tasik Ria as well as testing the antibacterial activity of extracts from some of the sponge against the bacteria Escherichia coli and Staphylococcus aureus. From the identification, seven species of sponges were found, which consists of: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Cribochalina sp., Hyrtios sp., and Lendenfeldia sp. The tests of antibacterial activity of the extracts from these sponges against test bacteria E. coli and S. aureus showed some positive results. Extract from Axinosa sp. sponge(16 mm) showed the strongest antibacterial activity on Escherichia coli bacteria. Followed by Hyrtios sp. extract (13.5 mm), Aaptos sp. extract (13 mm), Lendenfeldia sp. extract (13 mm) and Cribochalinai sp. extract(10.5 mm). While the the tests on Staphylococcus aureus bacteria showed that the strongest antibacterial activity was found from Axinosa sp. sponge extract (16.5 mm), followed by the extract from Aaptos sp. (15 mm), Lendenfeldia sp. extract (14.5 mm), Hyrtios sp. extract(13.5 mm) and Cribochalina sp. extract (11 mm).Keywords: Sponge, antibacterial, Escherichia coli, Staphylococcus aureus ABSTRAK Spons merupakan salah satu biota laut yang sangat prospektif sebagai sumber senyawa bahan-bahan alami antara lain peptide, terpenoid, steroid, asetogenin, alkaloid, halide siklik dan senyawa nitrogen. Penelitian ini diarahkan untuk mendapatkan beberapa spesies spons dari perairan Tasik Ria serta menguji aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri Escherichia coli dan bakteri Staphylococcus aureus. Hasil identifikasi spons ditemukan sebanyak tujuh spesies yang terdiri dari: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Hyrtios sp., Cribochalina sp. dan Lendenfeldia sp.. Aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri uji E. coli dan S. aureus terdapat diameter zona hambat bervariasi yaitu bakteri Escherichia coli menunjukkan aktivitas antibakteri ekstrak spons terkuat pada spons Axinosa sp (16 mm), disusul ekstrak spons Hyrtios sp. (13,5 mm), ekstrak spons Aaptos sp. (13 mm), ekstrak spons Lendenfeldia sp. (13 mm) dan ekstrak spons Cribochalinai sp. (10,5 mm).  Sedangkan pada bakteri Staphylococcus aureus menunjukkan aktivitas antibakteri ekstrak spons terkuat yaitu:  ekstrak spons Axinosa sp. (16,5 mm), disusul ekstrak spons Aaptos sp. (15 mm), ekstrak spons Lendenfeldia sp. (14,5 mm), ekstrak spons Hyrtios sp. (13,5 mm) dan ekstrak spons Cribochalina sp.(11mm).Kata Kunci : Spons, Antibakteri, Escherichia coli, Staphylococcus aureus

scholarly journals The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 3 (1) ◽  
pp. 56-63
Author(s):  
Julia Nanda Puspita ◽  
Rikhsan Kurniatuhadi ◽  
Rahmawati Rahmawati
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
And Staphylococcus Aureus

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of  E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).

scholarly journals KARAKTERISASI DAN PENAPISAN AKTIVITAS ANTIBAKERI ISOLAT BAKTERI SIMBION Thurudilla lineolata DAN Phyllidiella pustulosa

2020 ◽  
Vol 8 (3) ◽  
pp. 27
Author(s):  
Kevien Marthin Doringin ◽  
Rosita AJ Lintang ◽  
Deiske A Sumilat
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Bacterial Isolate ◽  
Nutrient Agar ◽  
Antibacterial Screening ◽  
And Staphylococcus Aureus

Nudibranch are part of a class og Gastropods that do not have a shell and has a bright and striking colour. Nudibranch has different of bacteria and bioactive compounds contained therein for protect theselves from predators.The aim of this study were to isolate symbiotic bacterial drived from Thurudilla lineolata and Phyllidiella pustulosa, and to observe the antibacterial activity of bacterial isolate against Escherichia coli and Staphylococcus aureus. Isolation and culture of the symbiotic bacterial were made on Nutrient Agar. The antibacterial screening showed that Thrudilla lineolata and Phyllidiella pustulosasymbiotic bacteria were able to inhibit the growth of against Escherichia coli and Staphylococcus aureus Nudibranch merupakan bagian dari kelas Gastropoda yang tidak memiliki cangkang dan memiliki warna cerah dan mencolok. Nudibranch memiliki berbagai jenis bakteri dan senyawa bioaktif yang terkandung di dalamnya untuk melidungi diri dari predator. Tujuan dari penelitian ini yaitu untuk mendapatkan isolat bakteri yang bersimbion dengan Thrudilla lineolata dan Phyllidiella pustulosa, kemudian mengamati aktivitas antibakteri dari isolat bakteri tersebut terhadap Escherichia coli, dan Staphylococcus aureus. Isolasi dan kultur bakteri yang bersimbion dengan Thrudilla lineolata dan Phyllidiella pustulosa, dibuat pada media Nutrient Agar. Skrining aktivitas antibakteri menunjukkan isolat bakteri yang bersimbion dengan Thrudilla lineolata dan Phyllidiella pustulosa mampu menghambat pertumbuhan organisme uji Staphylococcus aureus dan Escherichia coli. Kata kunci: Nudibranch, Thrudilla lineolata, Phyllidiella pustulosa, bakteri, isolasi, antibakter

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI SPONS Mycale Vansoesti DARI PERAIRAN PULAU MANTEHAGE MINAHASA UTARA TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Staphylococcus aureus

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 889
Author(s):  
Brigita Michelle Luntungan ◽  
Defny S. Wewengkang ◽  
Erladys Rumondor
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Chloroform Fraction ◽  
Marine Biota ◽  
Methanol Fraction ◽  
A Value ◽  
Activity Test ◽  
And Staphylococcus Aureus

ABSTRACTSponges are multi-cell marine biota whose tissue and organ functions are very simple. In the development of medicine, sponges have been shown to contain active compounds as guiding compounds in the synthesis of the latest drugs. The purpose of this study was to determine the antibacterial activity of the extracts and fractions of the sponge Mycale vansoesti collected from the waters of Mantehage Island against Escherichia coli and Staphylococcus aureus bacteria. The sponge samples were extracted using the maceration method with 95% ethanol. The antibacterial activity test used the disc diffusion agar method of Kirby and Bauer. The results showed that Mycale vansoesti sponge produced antibacterial activity in all extracts and fractions used namely ethanol extract, chloroform fraction, n-hexane fraction, and methanol fraction against Escherichia coli and Staphylococcus aureus bacteria with a value of about 6.63-8.82 mm and included in the moderate category of inhibition, this is proven by the formation of a clear zone around the disc. Keywords: Mycale vansoesti, Antibacterial, Staphylococcus aureus, Escherichia coli ABSTRAKSpons merupakan biota laut multi sel yang fungsi jaringan dan organnya sangat sederhana. Dalam perkembangan pengobatan, spons terbukti mengandung senyawa aktif sebagai senyawa pemandu dalam sintesis obat-obatan terbaru. Tujuan dari penelitian ini untuk mengetahui adanya aktivitas antibakteri dari ekstrak dan fraksi spons Mycale vansoesti yang diperoleh dari perairan Pulau Mantehage terhadap bakteri Escherichia coli dan Staphylococcus aureus. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol 95%. Pengujian Aktivitas Antibakteri menggunakan metode difusi agar Kirby and Bauer. Hasil penelitian menunjukkan bahwa spons Mycale vansoesti menghasilkan aktivitas antibakteri pada semua ekstrak dan fraksi yang digunakan yaitu ekstrak etanol, fraksi kloroform, fraksi n-heksan, dan fraksi metanol terhadap bakteri Escherichia coli dan Staphylococcus aureus dengan nilai sekitar 6,63-8,82 mm dan termasuk dalam daya hambat kategori sedang. Hal ini dibuktikan dengan terbentuknya zona bening disekitar cakram. Kata Kunci : Mycale vansoesti, Antibakteri, Staphylococcus aureus, Escherichia coli

scholarly journals AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MENGKUDU (Morinda citrifolia L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 505
Author(s):  
Aprilia Aslah ◽  
Widya A. Lolo ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Morinda Citrifolia ◽  
Diffusion Method ◽  
Methanol Fraction ◽  
Fractionation Method ◽  
Liquid Fractionation ◽  
Escherichia Coli Bacteria

ABSTRACT Noni is a nutritious plant because it has several ingredients that are important for health. Antibacterial components in noni leaves include glycosides, acubins, saponins and flavonoids. The purpose of this study was to determine the fraction of the extract of noni leaf ethanol whether it has an antibacterial effect and know the class of compounds indentifed as having antibacterial activity after TLC-Bioautografi testing was carried out. The samples were extracted using maceration method with 96% ethanol solvent and fractionation using liquid-liquid fractionation method with methanol, ethyl acetat and n-hexane solvents. Antibacterial activity testing was carried out with concentration variants of 20%, 30% and 40% with agar diffusion method (Kirby and Bauer). The results showed that methanol fraction, ethyl acetat fraction and Noni hexane leaf fraction effectively inhibited Staphylococcus aureus and Escherichia coli bacteria. The largest fraction of inhibiting zone was the concentration of 40% methanol which was categorized as strong. TLC monitoring was carried out using the mobile phase of chloroform : n-hexane (2:1). Antibacterial activity testing carried out by contact bioautography method showed that there were spots on the TLC choromatogram, which produced inhibitory zones. Characteristics of blotches were performed with AlCl3 spotting and it was thought that the spots were flavonoids.  Keywords : Noni, Antibacterial, Staphylococcus aureus, Escherichia coli, TLC-Bioautografi.  ABSTRAK Mengkudu merupakan tanaman yang berkhasiat karena mempunyai beberapa kandungan senyawa yang penting bagi kesehatan tubuh. Komponen yang berkhasiat antibakteri dalam daun mengkudu antara lain adalah glikosida, acubin, saponin dan flavonoid. Tujuan dari penelitian ini adalah untuk mengetahui fraksi dari esktrak etanol daun mengkudu apakah memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT-Bioautografi. Sampel diekstraksi menggunakan metode maserasi dengan pelarut etanol 96% dan fraksinasi dengan metode fraksinasi cair-cair dengan pelarut metanol, etil asetat dan n-heksan. Pengujian aktivitas antibakteri dilakukan dengan varian konsentrasi 20%, 30% dan 40% dengan metode difusi agar (Kirby dan Bauer). Hasil penelitian menunjukan bahwa fraksi metanol, fraksi etil asetat dan fraksi n-heksan daun Mengkudu efektif menghambat bakteri staphylococcus aureus dan Escherichia coli. fraksi yang paling besar zona hambatnya yaitu fraksi metanol konsentrasi 30% yang dikategorikan kuat, dilakukan pemantauan KLT menggunakan fase gerak kloroform : n-heksan (2:1). Pengujian aktivitas antibakteri dilakukan dengan metode bioautografi kontak hasilnya menunjukkan terdapat bercak pada kromatogram KLT yang menghasilkan zona hambat. Karakteristik bercak dilakukan dengan penampak bercak AlCl3 dan diduga bahwa bercak tersebut Flavonoid. Kata kunci : Mengkudu, Antibakteri, Staphylococcus aureus, Escherichia coli , KLT-Bioautografi

scholarly journals ANTIBACTERIAL ACTIVITY OF CRUDE EXTRACTS OF Solanum incanum AGAINST Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 4 (2) ◽  
pp. e183
Author(s):  
David Mutisya Musyimi ◽  
Tracy Ann Ashioya ◽  
George Opande ◽  
William Omuketi Emitaro
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Solanum Incanum ◽  
Zones Of Inhibition ◽  
Ethanol Extracts ◽  
And Staphylococcus Aureus

Human pathogenic microorganisms have developed resistance in response to indiscriminative use of commercial drugs. Plants produce many secondary metabolites with microbiocidal activity hence their use in traditional medicine. Herbalists in Kenya use medicinal plants including Solanum incanum in treating microbial infections. Though S. incanum has been used to treat different diseases in humans and animals, there is little information on antimicrobial activities of its extracts against Escherichia coli and Staphylococcus aureus. In this study, phytochemical analysis and antibacterial activity of solanum incanum leaves, roots and seeds extracts were determined. Ethanolic and aqueous extracts of leaf, root and seed of concentrations 25, 50, 75 and 100, and amoxicillin 25 mg/ml (control) with three replications were used for antibacterial analysis by the agar-well diffusion method. The results were subjected to analysis of variance at P < 0.05. Phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, steroids and tannins. Solanum incanum exhibited significant antibacterial effect against the two test bacteria. Ethanol extracts were more active than extracts against the bacteria. Ethanol extracts at 100% inhibited growth of Staphylococcus aureus more than the Escherichia coli. The zones of inhibition for Staphylococcus aureus were 35.0±0.6 mm, 30.94±0.3 mm and 30.14±0.64mm for seed, root and leaves respectively.On the other hand, the zones of inhibition for Escherichia coliat 100% ethanol were 27.20±0.06, 23.14±0.12 and 21.0±0.4 seed, root and leaves respectively.The results validate the use of these plants in ethnomedicine and potential of this plant in treating infections caused by the two bacteria.

scholarly journals UJI AKTIVITAS ANTIBAKTERI JAMUR ENDOFIT PADA DAUN DAN BATANG TUMBUHAN KUMIS KUCING (Orthosiphon aristatus) TERHADAP BAKTERI Escherichia coli DAN staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 211
Author(s):  
Excel Pangouw ◽  
Jimmy Posangi ◽  
Widya Astuty Lolo ◽  
Robert Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Antibacterial Effect ◽  
E Coli ◽  
Average Value ◽  
Escherichia Coli Bacteria ◽  
Cat’S Whiskers ◽  
And Staphylococcus Aureus

ABSTRACTEndophytic fungi are fungi that live in plant tissues and do not harm the plants. Endophytic fungi can produce compounds that have the potential to be antibacterial. This study aims to examine the antibacterial activity of endophytic fungi isolated from the leaves and stems of the cat's whiskers plant (Orthosiphon aristatus) against Escherichia coli and Staphylococcus aureus bacteria. Cat's whiskers contain essential oils which are used as antibacterial against some pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. The method used is experimentally in the laboratory to test the antibacterial activity of endophytic fungi isolates derived from cat's whiskers (Orthosiphon aristatus) on Escherichia coli and Staphylococcus aureus bacteria using the hole / well method. In the research results obtained four samples of endophytic fungi extract isolated from the leaves and stems of the cat's whiskers plant (Orthosiphon aristatus) . The results of antibacterial research showed that endophytic mushroom extract isolated from cat's whiskers were more effective in inhibiting Escherichia coli bacteria compared to Staphylococcus aureus. In Escherichia coli bacteria the average value of inhibition is 5 mm whereas in Staphylococcus aureus bacteria the average value of inhibition is only 2 mm. So it can be seen that the endophytic fungus from the leaves of cat's whiskers has a better antibacterial effect than the endophytic fungus from the stems of the cat's whiskets. Conclusion, etidophytic fungi isolated from leaves and stems of cat's whiskers (Orthosiphon aristatus) have an antibacterial effect on the growth of E. coli bacteria and are not effective against S. aureus bacteria.Keywords: Cat's whiskers (Orthasiphan aristatus), antibacterial activity, Escherichia coli and Staphylococcus aureus. ABSTRAKJamur endofit ialah jamur yang hidup di dalam jaringan tumbuhan dan tidak membahayakan tumbuhan tersebut. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri jamur endofit yang diisolasi dari daun dan batang tumbuhan kumis kucing (Orthosiphon aristatus) terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Kumis kucing mengandung minyak atsiri yang digunakan sebagai antibakteri terhadap beberapa bakteri patogen seperti Staphylococcus aureus dan Escherichia coli. Metode yang dipakai yakni secara eksperimen di laboratorium untuk menguji adanya aktivitas antibakteri dari isolat jamur endofit  yang diberasal dari tumbuhan kumis kucing (Orthosiphon aristatus)  pada bakteri  Escherichia coli dan Staphylococcus aureus dengan menggunakan metode lubang/sumuran. Pada hasil penelitan didapatkan empat sampel ekstrak jamur endofit yang diisolasi dari daun dan batang  tumbuhan kumis kucing (Orthosiphon aristatus). Hasil penelitian antibakteri menunjukkan bahwa ekstrak jamur endofit yang diisolasi dari tumbuhan kumis kucing lebih efektif menghambat  bakteri Escherichia coli dibandingkan dengan bakteri Staphylococcus aureus. Pada bakteri  Escherichia coli nilai rata-rata daya hambat yaitu 5 mm sedangkan pada bakteri Staphylococcus aureus nilai rata-rata daya hambat yaitu hanya 2 mm. Sehingga dapat diketahui bahwa jamur endofit dari daun kumis kucing memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit dari batang kumis kucing. Kesimpulan, Jamur endofit yang diisolasi dari daun  dan batang kumis kucing (Orthosiphon aristatus) memiliki efek antibakteri terhadap pertumbuhan bakteri E. coli dan tidak efektiv terhadap bakteri S. aureus.Kata Kunci:  Tumbuhan kumis kucing (Orthosiphon aristatus), aktivitas antibakteri, Escherichia coli dan Staphylococcu saureus.

ISOLASI DAN SKRINING AKTIVITAS ANTIBAKTERI DARI BAKTERI SIMBION NUDIBRANCHIA Phyllidiella pustulosa dan Thuridilla lineolata

2020 ◽  
Vol 8 (2) ◽  
pp. 40
Author(s):  
Ade Kurniawan Arie ◽  
Rosita A J Lintang ◽  
Remy E P Mangindaan ◽  
Agung B Windarto ◽  
Fitje Losung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Symbiotic Bacteria ◽  
Bacterial Isolates ◽  
Defense Systems ◽  
Self Defense ◽  
Solid Media ◽  
Antibacterial Assay ◽  
And Staphylococcus Aureus

Nudibranchs are marine invetebrates belongs to the class of Gastropoda that are able to camouflage and develop a self-defense systems. Marine bacteria contain chemicals compunds that have potentials on marine drugs discovery through the secondary metabolism. The purpose of this study was to isolate the symbiotic bacteria from nudibranches Phylidiella pustulosa and Thuridilla lineolate and to screen the antibacterial activity of these bacterial isolates against Escherichia coli and Staphylococcus aureus. Isolation and culture of the symbiotic bacteria was made on B1 (solid) media. The results of the antibacterial assay showed that the symbiotic bacterial isolates from Phylidiella pustulosa and Thuridilla lineolate were able to inhibit the growth of the Staphylococcus aureus and Escherichia coli test. Keywords: nudibranch, Phyllidiella pustulosa, Thuridilla lineolate, bacteria, antibacterialAbstrakNudibranch adalah avetebrata laut dalam kelas Gastropoda yang mampu melakukan kamuflase dan mengembangkan sistem pertahanan diri. Bakteri laut mengandung senyawa kimia yang berpotensi sebagai bahan obat-obatan yang dihasilkan melalui metabolit sekunder. Tujuan dari penelitian ini yaitu untuk mengisolasi bakteri yang bersimbion dengan nudibranch Phylidiella pustulosa dan Thuridilla lineolate, kemudian mengamati aktivitas antibakteri dari isolat bakteri tersebut terhadap Escherichia coli danStaphylococcus aureus. Isolasi dan kultur bakteri yang bersimbion dibuat pada media B1 (padat). Skrining aktivitas antibakteri menunjukkan isolat bakteri yang bersimbion dengan nudibranch Phylidiella pustulosa dan Thuridilla lineolate mampu menghambat pertumbuhan organisme uji Staphylococcus aureus dan Escherichia coli. Kata kunci: nudibranch, Phyllidiella pustulosa, Thuridilla lineolate, bakteri, antibakteri

AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MANGGIS (Garcinia mangostana L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 525
Author(s):  
Ayu Natasya Paputungan ◽  
Widya Astuty Lolo ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Garcinia Mangostana ◽  
Methanol Fraction ◽  
Tlc Bioautography ◽  
Fractionation Method

Mangosteen leaves have flavonoid compounds, tannins, and saponins that can be efficacious as antibacterial. The aim of this study was to determine the fraction of mangosteen leaves having an antibacterial effect and knowing the class of compounds identified as having antibacterial activity after TLC- Bioautography testing was carried out. The samples were extracted using 96% maceratarion method and fractioned using liquid-liquid fractionation method with methanol, n-hexane and ethyl acetate solvents, antibacterial activity using agar diffusion method (Kirby and Bauer) with 3 concetrations namely 10%, 20% and 30%. Thin Layer Chromatography (TLC) uses n-hexane and chloroform solvens. TLC-Bioautography uses contact bioautography methods. The resultd showed that mangosteen leaves in methanol fraction with a concentration of 30% had a very large inhibitory activity again Staphylococcus aureus and ethyl acetate fraction with a concentration of 30% had the gratest antibacterial activity against  Escherichia coli. The results of the TLC- Bioautography study showed that the flavonoids compounds after spraying with AlCl3 and the mangosteen leaf Biosutography test had inhibitory zone activity against the bacteria Staphylococcus aureus dan Escherichia coli. Keywords: Mangosteen Leaves. Antibacterial, TLC Bioautography.  ABSTRAK Daun manggis mempunyai senyawa flavonoid, tanin, dan saponin yang dapat berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui fraksi daun manggis memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT Bioautografi. Sampel diektrak dengan metode maserasi dengan pelarut 96% dan difraksinasi dengan metode  fraksinasi cair-cair dengan pelarut metanol, n-heksan dan etil asetat, aktivitas antibakteri menggunakan metode difusi agar (Kirby and Bauer) dengan 3 kosentrasi yaitu 10%, 20% dan 30%. Kromatografi Lapis Tipis (KLT) menggunakan pelarut n-heksan dan klorofom. KLT-Bioautografi menggunakan metode bioautografi kontak. Hasil penelitian menunjukan daun manggis pada fraksi metanol  dengan kosentrasi 30% memiliki aktivitas zona hambat ppaling besar terhadapat bakteri Staphylococcus aureus dan fraksi etil asetat dengan kosentrasi 30% memiliki aktivitas antibakteri paling besar terhadap  Escherichia coli. Hasil penelitian KLT-Bioautografi menunjukan golongan senyawa flavonoid setelah disemprotkan dengan AlCl3 dan uji Bioautografi daun manggis memiliki aktivitas zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kata kunci : Daun Manggis, Antibakteri, KLT- Bioautografi.

Sign in / Sign up

Export Citation Format

Share Document