The Effect of Cold Atmospheric Plasma on the Expression of Apoptotic Genes in Breast Cancerous Cells

2021 ◽  
Author(s):  
Hadis Ahmadirad ◽  
Mahdi Shariat ◽  
Mahdi Mahmoodi ◽  
Soudeh Falahatipour ◽  
Mohammad Reza Hajizadeh ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cancer Cell Line ◽  
Atmospheric Plasma ◽  
Apoptosis Pathway ◽  
Anti Cancer ◽  
Helium Gas ◽  
Apoptotic Genes ◽  
Apoptosis And Necrosis ◽  
Cancerous Cells ◽  
Mcf 7

Background: Breast cancer is one of the most malignant cancers in the world. Cold micro plasma jet (CMPJ) Known as cold atmospheric jet microplasma, it has recently been introduced as an alternative way to overcome the challenges of finding an effective cancer treatment. Numerous studies have reported promising results, so our aim of this study was to investigate how this method affects cell death and its role on the expression of apoptotic genes in the MCF-7 cancer cell line. Methods: In this study, helium gas was used to generate plasma at room temperature in the form of point radiation at different times of 30, 60, 90 and 120 seconds and at different distances of 1 cm. Flow cytometry will be used to examine the extent of apoptosis and necrosis. Genes involved in apoptosis P53, P21, Bax and Bcl-2 were measured by real-time PCR. Results: Our Studie indicate that the mechanism of action of cold plasma on cancer cells is related to generation of reactive oxygen species with possible induction of the apoptosis pathway. The percentages of necrotic and late apoptotic cells following treatment with different times plasma (0, 30, 60, 90 and 120 s) were about 0.55 ± 0.06, 20.13 ± 0.01, 20.12 ± 0.03, 26.81 ± 0.04 and 17.51 ± 0.05. The mRNA expression of bcl-2 showed a decrease of 90s of plasma while the mRNA expression of p53, bax and caspase-8 genes increased compared to untreated cells. Conclusion: In general, research in the last decade has confirmed the ability of CMPJ as an effective anti-cancer tool. Therefore, it may be used to help treat cancer. However, its clinical application requires much further studies to determine the severity and duration of exposure to CMPJ for effective treatment based on the type of cancer.

scholarly journals Increased vimentin mRNA expression in MCF-7 breast cancer cell line after repeated endoxifen-treatment

2017 ◽  
Vol 25 (4) ◽  
pp. 207-13 ◽  
Author(s):  
Paramita Paramita ◽  
Melva Louisa ◽  
Nafrialdi Nafrialdi
Keyword(s):  
Breast Cancer ◽  
Mrna Expression ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Intermediate Filament Protein ◽  
Mesenchymal Transition ◽  
Mcf 7

Background: Epithelial mesenchymal transition (EMT) plays a significant role in the development of cancer cell resistance to drugs. Vimentin, a type III intermediate filament protein, is a marker of EMT. Vimentin's over-expression in cancer correlates well with increased tumor growth, change in cell shape and poor prognosis. Endoxifen is an active metabolite of tamoxifen  and has become a new potent agent in the treatment of breast cancer. This is a study that aimed to investigate the effect of endoxifen exposure with or without estradiol on cell viability, cell morphology and EMT progression through the analysis of vimentin mRNA expression after 4-week treatment. Methods: Endoxifen, 100 nM or 1,000 nM, with or without beta-estradiol were given repeatedly to MCF-7 cells. Cells treated with dimethyl sulfoxide (DMSO) 0.001% were used as control. After 2- and 4-week exposure, the cells were counted, analyzed for mRNA vimentin expression, and observed for morphological changes. Results: Compared to control, there were significant decreases in vimentin mRNA expressions in endoxifen and endoxifen+β-estradiol treated cells after 2-weeks, which then significantly increased after 4-week compared with the 2-week exposure. We found no change in morphology of MCF-7 cells. Conclusion: Repeated exposure of endoxifen might induce EMT progression through increased expression of vimentin in MCF-7 breast cancer cell line.

Curcumin Increases Anti-Cancer Activity of Tamoxifen in MCF-7 Breast Cancer Cells Through the Suppression of MDR1 mRNA Expression

2017 ◽  
Vol 23 (7) ◽  
pp. 6838-6840
Author(s):  
Melva Louisa ◽  
Lies Sugiarti ◽  
Sandy Vitria Kurniawan ◽  
Septelia Inawati Wanandi
Keyword(s):  
Breast Cancer ◽  
Mrna Expression ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Anti Cancer ◽  
Mdr1 Mrna ◽  
Cancer Activity ◽  
Mcf 7

scholarly journals Anticancer Activities of Chemical Constituents from Leaves and Twigs of Mitrephora winitii

2021 ◽  
Vol 21 (3) ◽  
pp. 699
Author(s):  
Sukee Sukdee ◽  
Puttinan Meepowpan ◽  
Narong Nantasaen ◽  
Siriporn Jungsuttiwong ◽  
Sarinya Hadsadee ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Chemical Constituents ◽  
Cancer Cell Line ◽  
2D Nmr ◽  
Anticancer Activities ◽  
Chromatographic Separations ◽  
Chemical Structures ◽  
Anti Cancer ◽  
First Time ◽  
Mcf 7

The genus Mitrephora has been investigated and its anti-inflammatory, anti-bacterial and anti-parasitical activities were examined along with its potential as an anti-cancer cell line and inhibitor for platelet aggregation. In this work, air-dried leaves and twigs of M. winitii were grounded and extracted with n-hexane, ethyl acetate and methanol, respectively. Chromatographic separations of these extracts led to the isolation of three known compounds and one new compound (compound 2). The chemical structures of these were identified using spectroscopic investigation of 1D- and 2D-NMR and the resulting data confirmed these as stigmasterol (1), (3,4-dimethoxyphenyl)(5-(3,4-dimethoxyphenyl)-4-(hydroxymethyl)tetrahydrofuran-3-yl)methanol (2), diayangambin (3), and methyl-L-inositol (4). The chemical constituents were reported the first time in M. winitii. Compound 2 showed anti-cancer cell lines with ED50 13.07 µg/mL against KB cells and then was tested for cytotoxicity against MCF-7 cells with ED50 11.77 µg/mL.

Resveratrol induces apoptosis and attenuates proliferation of MCF-7 cells in combination with radiation and hyperthermia.

2020 ◽  
Vol 20 ◽  
Author(s):  
Peyman Amini ◽  
Saeedeh Jafari Nodooshan ◽  
Milad Ashrafizadeh ◽  
Seyed-Mohammad Eftekhari ◽  
Tayebeh Aryafar ◽  
...  
Keyword(s):  
Cancer Cells ◽  
In Vitro Study ◽  
Cancer Cell Death ◽  
Radiotherapy Alone ◽  
Apoptotic Genes ◽  
Effects Of Radiation ◽  
Sensitizing Effect ◽  
Apoptosis And Necrosis ◽  
Mcf 7

Aim: In the current in vitro study, we tried to examine the possible role of resveratrol as a sensitizer in combination with radiotherapy or hyperthermia. Background: Breast cancer is the most common malignancy for women and one of the most common worldwide. It has been suggested that using non-invasive radiotherapy alone cannot eliminate cancer cells. Hyperthermia which is an adjuvant modality induces cancer cell death mainly through apoptosis and necrosis. However, cancer cells can also develop resistance to this modality. Objective: The objective of this study was to determine possible potentiation of apoptosis when MCF-7 cells treated with resveratrol before hyperthermia or radiotherapy. Method: MCF-7 cancer cells were treated with different doses of resveratrol to achieve IC50%. Afterwards, cells treated with the achieved concentration of resveratrol were exposed to radiation or hyperthermia. Proliferation, apoptosis and the expression of pro-apoptotic genes were evaluated using flow cytometry, MTT assay and real-time PCR. Results for each combination therapy were compared to radiotherapy or hyperthermia without resveratrol. Results: Both irradiation or hyperthermia could reduce viability of MCF-7 cells. Furthermore, the regulation of Bax and caspase genes increased, while Bcl-2 gene expression reduced. Resveratrol potentiated the effects of radiation and hyperthermia on MCF-7 cells. Conclusion: Results of this study suggest that resveratrol is able to induce the regulation of pro-apoptotic genes and attenuate the viability of MCF-7 cells. This may indicate the sensitizing effect of resveratrol in combination with both radiotherapy and hyperthermia.

Synthesis and Apoptotic Activities of New 2(3H)-benzoxazolone Derivatives in Breast Cancer Cells

2020 ◽  
Vol 21 (1) ◽  
pp. 84-90
Author(s):  
Emine Erdag ◽  
Eda Becer ◽  
Yusuf Mulazim ◽  
Hafize Seda Vatansever ◽  
Hilal Kabadayı ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytochrome C ◽  
Caspase 3 ◽  
Biological Activities ◽  
Cancer Cell Line ◽  
Mannich Bases ◽  
Control Group ◽  
Anti Cancer ◽  
Fas L ◽  
Mcf 7

Background: 2(3H)-Benzoxazolone derivatives are preferential structural blocks in pharmacological probe designing with the possibility of modifications at various positions on the core structure. Benzoxazolones showed various biological activities such as analgesics, anti-inflammatory and anti-cancer. Objective: In the present work, we have prepared new Mannich bases of 2(3H)-benzoxazolone derivatives and evaluated their cytotoxicities and proapoptotic properties in MCF-7 breast cancer cell line. Methods: The structures of these compounds were characterized by FT-IR, elemental analysis, 1H and 13C NMR. Cytotoxicities of all the target compounds were investigated by MTT assay. Apoptotic properties of compounds were evaluated by immunocytochemistry using antibodies against caspase-3, cytochrome-c, FasL, and also TUNEL assay. Results: These two novel compounds, 1 and 2, both have the same piperazine substituent on the nitrogen atom of benzoxazolone and the main difference in the structures of these compounds is the presence of Cl substituent at the 5- position of the benzoxazolone ring. MTT results showed that compounds 1 and 2 were effective in terms of reduction of cell viability at 100μM and 50μM concentration for 48h, respectively. As a result of immunohistochemical staining, Fas L and caspase-3 immunoreactivities were significantly increased in MCF-7 cells after treatment with compound 1. Additionally, caspase-3 and cytochrome-c immunoreactivities were also increased significantly in MCF-7 cells after treatment with compound 2. The number of TUNEL positive cells was significantly higher in MCF-7 cells when compared with the control group after treatment with both compounds 1 and 2. Conclusion: It could be concluded that N-substituted benzoxazolone derivatives increase potential anti-cancer effects and they could be promising novel therapeutic agents for chemotherapy.

scholarly journals Synthesis, Characterization, and Anticancer Activity of New Quinazoline Derivatives against MCF-7 Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Fadhil Lafta Faraj ◽  
Maryam Zahedifard ◽  
Mohammadjavad Paydar ◽  
Chung Yeng Looi ◽  
Nazia Abdul Majid ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Anticancer Activity ◽  
Cancer Cell Line ◽  
Mitochondrial Pathway ◽  
Potential Candidate ◽  
Significant Activity ◽  
Cytochrome C Release ◽  
Apoptosis Pathway ◽  
Mcf 7

Two new synthesized and characterized quinazoline Schiff bases 1 and 2 were investigated for anticancer activity against MCF-7 human breast cancer cell line. Compounds 1 and 2 demonstrated a remarkable antiproliferative effect, with an IC50value of 6.246 × 10−6mol/L and 5.910 × 10−6mol/L, respectively, after 72 hours of treatment. Most apoptosis morphological features in treated MCF-7 cells were observed by AO/PI staining. The results of cell cycle analysis indicate that compounds did not induce S and M phase arrest in cell after 24 hours of treatment. Furthermore, MCF-7 cells treated with 1 and 2 subjected to apoptosis death, as exhibited by perturbation of mitochondrial membrane potential and cytochrome c release as well as increase in ROS formation. We also found activation of caspases-3/7, -8, and -9 in compounds 1 and 2. Moreover, inhibition of NF-κB translocation in MCF-7 cells treated by compound 1 significantly exhibited the association of extrinsic apoptosis pathway. Acute toxicity results demonstrated the nontoxic nature of the compounds in mice. Our results showed significant activity towards MCF-7 cells via either intrinsic or extrinsic mitochondrial pathway and are potential candidate for furtherin vivoand clinical breast cancer studies.

scholarly journals Computing Vertex Degree-Based Multiplicative Version of Topological Indices for Tungsten Trioxide Nano Multilayer Structure in Nanotherapeutic Anti-Cancer Activity

2021 ◽  
Vol 12 (2) ◽  
pp. 2275-2284
Keyword(s):  
Chemical Structure ◽  
Chemical Compounds ◽  
Topological Indices ◽  
Vertex Degree ◽  
Anti Cancer ◽  
Cancerous Cells ◽  
Insight Into ◽  
Cancer Activity ◽  
Mcf 7 ◽  
Chemical Materials

Several research reports suggest that there is a strong interdependence among the molecular structure of chemical compounds and their physicochemical properties. The computation of the topological index of such a chemical structure facilitates researchers to gain more insight into the physical and bio-activity of chemical materials. In this article, we focus on WO3, which is a widely studied nanomaterial and is recently employed as an excellent cytotoxic agent towards the liver (Hep–2) and the breast (MCF–7) cancerous cells. Various vertex degree-based multiplicative versions of topological indices for WO3 nano multilayer were computed using the edge partition technique. We also compared all of the indices graphically. The obtained results redress the lack of medical and chemical experiments, thus constructing the theoretical framework for the pharmacological field.

scholarly journals Anti-Cancer Effect of Angelica Sinensis on Women’s Reproductive Cancer

2012 ◽  
Vol 2 (6) ◽  
pp. 242 ◽  
Author(s):  
Hong-Hong Zhu ◽  
Guo-Hui Huang ◽  
Patricia L. Tate ◽  
Lyndon L. Larcom
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Uv Light ◽  
Cancer Cell Line ◽  
Cancer Cell Lines ◽  
Dose Effect ◽  
Angelica Sinensis ◽  
Reproductive Disorders ◽  
Anti Cancer ◽  
Mcf 7

Objective: Danggui, the root of Angelica Sinensis, has traditionally been used for the treatment of women’s reproductive disorders in China for thousands of years. This study was to determine whether Danggui have potential anti-cancer effect on women’s cancer and its potential mechanism. Methods: Danggui was extracted by ethanol. The Cell Titer 96® Aqueous Non-Radioactive Cell Proliferation Assay was used to compare the effects of Danggui on human breast (MCF-7 and 7368) and cervical (CaSki and SiHa) cancer cells with its effects on normal fibroblasts (HTB-125). A revised Ames test was used to test for antimutagenicity. The standard strains of Salmonella typhimarium (TA) 100 and 102 were used in the test. Methyl methane sulfonate (MMS) and UV light were used as positive mutagen controls and ethanol and double distilled water (DDW) as controls. The SAS statistical software was used to analyze the data. Results: Danggui was found to be much more toxic to all cancer cell lines tested than to normal fibroblasts. There was a significant negative dose-effect relationship between Danggui and cancer cell viability. Average viability of MCF-7 was 69.5%, 18.4%, 5.7%, 5.7%, and 5.0% of control for Danggui doses 0.07, 0.14, 0.21, 0.32, and 0.64 ug/ul, respectively, with a Ptrend < 0.0001. Half maximal inhibitory dose (ID50) of Danggui for cancer cell lines MCF-7, CaSki, SiHa and CRL-7368 was 0.10, 0.09, 0.10 and 0.07 ug/ul, respectively. For the normal fibroblasts, ID50 was 0.58 ug/ul. At a dose of 0.32 ug/ul, Danggui killed over 90% of the cells in each cancer cell line, but at the same dose, only 12.3 % of the normal HTB-125 cells were killed. Revertants per plate of TA 100 decreased with the introduction of increasing doses of Danggui extracts with a Ptrend < 0.0001 when UV light was used as a mutagen. There was no difference in revertants per plate between ethanol and DDW control groups. Conclusions: Danggui could be used as a safe and effective adjuvant therapy to prevent and treat breast and cervical cancers. Anti-cancer effects may be due to its anti-mutagenicity. Danggui should be investigated as a potential adjuvant anti-cancer therapy for women’s cancer treatment and prevention of recurrence. Key words: Angelica Sinensis, Danggui, cancer, women’s reproductive disorders

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