scholarly journals Preliminary Study of the Mosquito Repellent and Adulticidal Effects of Volatile Oils of Lemon grass (Cymbopogon winterianus) in Imo State, Southeast Nigeria

2020 ◽  
Vol 7 (01) ◽  
pp. 4726-4734
Author(s):  
Emmanuel Nwanya ◽  
Evangeline Tochi Oparaocha ◽  
Ikenna Emmanuel Okoroafor ◽  
Christopher Sule Oyamienlen
Keyword(s):  
Controlled Trial ◽  
Volatile Oil ◽  
Body Parts ◽  
Mosquito Repellent ◽  
Complete Protection ◽  
Volatile Oils ◽  
Protection Time ◽  
Lemon Grass ◽  
Biting Rate ◽  
Close Range

Lemon grass (Cymbopogon winteriatus) has been widely acclaimed to possess therapeutic, cosmetic and insecticidal activities. This study was conducted using a field controlled trial by human volunteers to confirm the effectiveness and applicability of locally-produced Lemon grass oil as a mosquito repellent and adulticidal agent for the prevention of mosquito-borne diseases in Nigeria. Volatile oil was extracted using petroleum ether as solvent while olive oil was used as oil base. The study was conducted between September and October 2017 and made use of 12 volunteers across three locations. The lotions were applied on exposed body parts of the volunteers and they worked three shifts daily. The biting rate, percentage repellence complete protection time and whole night protection were measured and recorded. Topical application of each lotion reduced the biting rate of mosquitoes across the three locations, with the 60% (v/v) exhibiting higher impact of 341 vs 32; 438 vs 44 and 388 vs 33.  The 60% (v/v) formulation also had the highest percentage repellence of 91.92% at Location I, offered a whole night protection against mosquito in location I and O and offered a Complete Protection Time (CPT) of 21.6, 18.93 and 20.8 respectively across all three locations. There was a significant statistical association r=0.95 (p<0.05) between concentrations of the Lemon grass oil formulations and mean duration protection time against mosquitoes. All formulation exhibited adulticidal activities, with the 60% (v/v) formulation having higher impact, causing fast immobilization or paralyzing effect on some mosquitoes that were at close range to treated volunteer’s body. The study concludes that volatile oils of Lemon grass possess mosquito (A. gambiae and An. funestus) repellent and adulticidal effects, especially at higher concentrations and can be used to reduce human-mosquito contacts and hence mosquito-borne diseases and irritation caused by their bites.

Comparative Study of a and b- pinene Content in Volatile Oils of Abies alba, Pinus sylvestris, Juniperus communis, Rosmarinus officinalis, Salvia officinalis and Coriandrum sativum

2018 ◽  
Vol 69 (9) ◽  
pp. 2338-2342
Author(s):  
Mariana Popescu ◽  
Diana Puiu ◽  
Anca Daniela Raiciu
Keyword(s):  
Pinus Sylvestris ◽  
Raw Materials ◽  
Abies Alba ◽  
Volatile Oil ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Coriandrum Sativum ◽  
Volatile Oils ◽  
Juniperus Communis ◽  
Chromatographic Profile

The study describes the composition of volatile oils obtained by steam distillation of water fir (Abies alba), pine (Pinus sylvestris), juniper (Juniperus communis), rosemary (Rosmarinus officinalis), sage (Salvia officinalis), and coriander (Coriandrum sativum) using the gas chromatography technique coupled with mass spectrometry (GC-MS). Chromatographic profile revealed the presence of specific components for each studied volatile oil. It noted the presence of a and b- pinene in all volatile oils studied at different concentrations. Volatile oils studied, from green sources and rich in a, b-pinene will be used as raw materials for creating products with anti-inflammatory, antiseptic, bronchodilators properties.

scholarly journals Allelopathic Effect of Serphidium kaschgaricum (Krasch.) Poljak. Volatiles on Selected Species

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 495
Author(s):  
Shixing Zhou ◽  
Toshmatov Zokir ◽  
Yu Mei ◽  
Lijing Lei ◽  
Kai Shi ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Volatile Oil ◽  
Amaranthus Retroflexus ◽  
Allelopathic Effect ◽  
Chemical Profile ◽  
Volatile Oils ◽  
Phytotoxic Activity ◽  
Volatile Organic ◽  
First Time ◽  
Stem Leaf

The chemical profile and allelopathic effect of the volatile organic compounds (VOCs) produced by a dominant shrub Serphidium kaschgaricum (Krasch.) Poljak. growing in northwestern China was investigated for the first time. Serphidium kaschgaricu was found to release volatile compounds into the surroundings to affect other plants’ growth, with its VOCs suppressing root elongation of Amaranthus retroflexus L. and Poa annua L. by 65.47% and 60.37% at 10 g/1.5 L treatment, respectively. Meanwhile, volatile oils produced by stems, leaves, flowers and flowering shoots exhibited phytotoxic activity against A. retroflexus and P. annua. At 0.5 mg/mL, stem, leaf and flower oils significantly reduced seedling growth of the receiver plants, and 1.5 mg/mL oils nearly completely prohibited seed germination of both species. GC/MS analysis revealed that among the total 37 identified compounds in the oils, 19 of them were common, with eucalyptol (43.00%, 36.66%, 19.52%, and 38.68% in stem, leaf, flower and flowering shoot oils, respectively) and camphor (21.55%, 24.91%, 21.64%, and 23.35%, respectively) consistently being the dominant constituents in all oils. Eucalyptol, camphor and their mixture exhibited much weaker phytotoxicity compared with the volatile oils, implying that less abundant compounds in the volatile oil might contribute significantly to the oils’ activity. Our results suggested that S. kaschgaricum was capable of synthesizing and releasing allelopathic volatile compounds into the surroundings to affect neighboring plants’ growth, which might improve its competitiveness thus facilitate the establishment of dominance.

scholarly journals Volatile oils analysis of fern (Pteridophyte) around Pangajaran waterfalls, Wonosalam, Jombang

2005 ◽  
Vol 3 (1) ◽  
pp. 22-25
Author(s):  
YUYUN MARINI ◽  
SUTARNO SUTARNO ◽  
AHMAD DWI SETYAWAN
Keyword(s):  
Field Survey ◽  
Microscopic Analysis ◽  
Volatile Oil ◽  
Morphological Characters ◽  
Volatile Oils ◽  
Mesophyll Tissue ◽  
Fern Species ◽  
Oil Concentration ◽  
Percentage Similarity ◽  
Leaf P

The aims of the research were: to know species diversity of fern (Pteridophyte) from Pangajaran, Wonosalam, Jombang, to know fern species containing volatile oil, to know concentration and percentage similarity of substances and characteristics of the substances containing in the oil, and to know the structure of cell producing volatile oil in trees and leaf of the fern. Fern diversity was studied by field survey, volatile oil concentration measured by hydro-distillation followed with gas chromatography to further know the components in the oil, while structure of the cell producing volatile oil was detected cross section of the trees and leaf for microscopic analysis. Based on the data and analysis result can be concluded that there were 13 fern species in Pangajaran. Two of the 13 species were confirmed as producing volatile oil, Pteris beaurita Linn. and Cyathea contaminans, that were produced volatile on their leaf only. Concentration of volatile oil of leaf P. beaurita was 0,005%, while in C. contaminans 0,01%. Percentage similarity of the volatile oil between two species based on its Retention Time (RT) was 2,5%, at the RT point of 21.247 in P. beaurita and at RT point of 21.294 in C. contaminans. Percentage similarity of both species based on morphological characters was 36.36%. Location of volatile oil producing cells in both species of fern was spreadly dispersed in schlerenchyma tissue and in mesophyll tissue of the leaf.

scholarly journals Chemical Composition and in Vitro Antimicrobial Activity of the Volatile Oils from Gliomastix murorum and Pichia guilliermondii, Two Endophytic Fungi in Paris polyphylla var. yunnanensis

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Jianglin Zhao ◽  
Tijiang Shan ◽  
Yongfu Huang ◽  
Xili Liu ◽  
Xiwu Gao ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Inhibitory Concentration ◽  
Octadecenoic Acid ◽  
Volatile Oil ◽  
Pichia Guilliermondii ◽  
Volatile Oils ◽  
Paris Polyphylla ◽  
Gliomastix Murorum

Volatile oils were obtained by hydrodistillation from Gliomastix murorum and Pichia guilliermondii, two endophytic fungi isolated from the traditional Chinese medicinal herb Paris polyphylla var. yunnanensis. The oils were analyzed for their chemical composition by gas chromatography-mass spectrometry (GC-MS). Palmitic acid (15.5%), (E)-9-octadecenoic acid (11.6%), 6-pentyl-5,6-dihydropyran-2-one (9.7%), and (7Z,10Z)-7,10- hexadecadienoic acid (8.3%) were the major compounds of the 40 identified components in G. murorum volatile oil. 1,1,3a,7-Tetramethyl-1a,2,3,3a,4,5,6,7b-octahydro-1H-cyclopropa[a]- naphthalene (25.9%), palmitic acid (15.5%), 1-methyl-2,4-di- (prop-1-en-2-yl)-1- vinylcyclohexane (7.9%), (E)-9-octadecenoic acid (7.3%), and (9E,12E)-ethyl-9,12-octadecadienoate (5.2%) were the major compounds of the 27 identified components in P. guilliermondii volatile oil. The in vitro antimicrobial activity of the volatile oils was also investigated to evaluate their efficacy against six bacteria and one phytopathogenic fungus. The minimum inhibitory concentration (MIC) values of the volatile oils against the test bacteria ranged from 0.20 mg/mL to 1.50 mg/mL. One of the most sensitive bacteria was Xanthomonas vesicatoria with an MIC of 0.20 mg/mL and 0.40 mg/mL for G. murorum and P. guilliermondii, respectively. The mean inhibitory concentration (IC50) of the volatile oils against spore germination of Magnaporthe oryzae was 0.84 mg/mL for G. murorum and 1.56 mg/mL for P. guilliermondii. These results indicated that the volatile oils from the endophytic fungi have strong antimicrobial activity and could be a potential source of antimicrobial ingredients.

Volatile oils and the study of natural hybridization between Correa aemula and C. reflexa (Rutaceae)

1977 ◽  
Vol 25 (3) ◽  
pp. 291 ◽  
Author(s):  
T Whiffin
Keyword(s):  
Numerical Analysis ◽  
Parental Species ◽  
Volatile Oil ◽  
Natural Hybridization ◽  
Volatile Oils ◽  
Hybrid Swarm ◽  
Hybrid Plants ◽  
Complex Variation

A small hybrid swarm between Correa aemula and C. reflexa was located and examined morphologically and chemically, with an emphasis on volatile oils. The hybrid plants were intermediate morphologically between the two parental species. The volatile oil data were subjected to multivariate numerical analysis, the results of which confirmed the general intermediacy of the hybrid plants. In general it was found that ordination is a more useful technique for the study of hybridization than is classification. It would appear that numerical analysis of volatile oil data will prove a useful technique in the study of the complex variation found within the genus Correa.

Insecticidal and Repellent Activities of Essential Oil from Leaves of Eucalyptus grandis Against Culex pipiens quinquefasciatus

2011 ◽  
Vol 233-235 ◽  
pp. 82-86 ◽  
Author(s):  
Yu Hong Tian ◽  
Xian Chuang Zhou ◽  
Xiao Liu Zhou ◽  
Qiong Huang
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Insecticidal Activity ◽  
Culex Pipiens ◽  
Eucalyptus Grandis ◽  
Gas Chromatography Mass Spectrometry ◽  
Complete Protection ◽  
Culex Pipiens Quinquefasciatus ◽  
Protection Time

The insecticidal and repellent activities of essential oil from leaves ofEucalyptus grandisagainstCulex pipiens quinquefasciatuswere investigated. The essential oil was obtained by hydro-distillation and analyzed by gas chromatography-mass spectrometry (GC-MS). The principal constituents in the essential oil werep-cymene (26.794%),α-pinene (15.864%), eucalyptol (6.281%),α-terpineol (4.100%), limonene (3.614%) and borneol (2.519%). The results indicated that the essential oil ofEucalyptus grandisleaves hold insecticidal and repellent activities againstCulex pipiens quinquefasciatus. The complete protection time for people againstCulex pipiens quinquefasciatuswas correlative with its concentration. It could protect people for 7h when its concentration was 50%. Fumigating insecticidal activity was evaluated by airtight fumigation in conical flask. The LC50values of the essential oil for adults ofCulex pipiens quinquefasciatuswere 4.5%(0.5h), 3.6%(1h), 2.6%(2h), 2.1%(4h), 0.56%(8h) and 0.56%(24h). The LT50value of the essential oil was 19.1 min when its concentration was 6.4%.

scholarly journals Atractylodes Lancea Volatile Oils Target ADAR2-miR-181a-5p Signaling to Mesenchymal Stem Cells Chondrogenic Differentiation

2021 ◽  
Author(s):  
Shanyu Ye ◽  
Wenwen Si ◽  
Wei Qin ◽  
Ziwei Luo ◽  
Zhen Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chinese Medicine ◽  
Chondrogenic Differentiation ◽  
Volatile Oil ◽  
Luciferase Reporter ◽  
Blue Staining ◽  
Reporter System ◽  
Volatile Oils ◽  
Atractylodes Lancea

Abstract Background：The Rhizoma Atractylodis has long been recommended for the treatment of different diseases in traditional Chinese medicine. The main component of Rhizoma Atractylodis is Atractylodes lancea volatile oils which possesses anti-microorganism, anti-tumor, cognitive protection and immunoregulation. The aim of the study was to elucidate the mechanism of Atractylodes lancea volatile oils promoting mesenchymal stem cells (MSCs) chondrogenic differentiation. Method：Atractylodes lancea volatile oils were extracted from Chinese medicine Cangzhu by volatile oil extractor. MSCs culture were treated with Atractylodes lancea volatile oils medium. Real-time reverse transcription PCR was conducted to verify the candidate microRNAs discovered by microarray analysis. Western-blot analyzed the expressions of mark genes. Sanger sequences identified the changes of the base pairs which would be edited by ADAR2 enzyme. Toluidine blue staining identified the changes of cells chondrogenic differentiation. Result：Treatment of Atractylodes lancea volatile oils increased the cells chondrogenic differentiation of MSCs. Atractylodes lancea volatile oils promoted the expression of ADAR2 enzyme which may edit the precursor of miR-181a-5p. A dual luciferase reporter system assay verified that transcription factors yingyang1(YY1) was targeted by miR-181a-5p which was downregulated in MSCs chondrogenic differentiation. Conclusion：This work demonstrates the mechanism of Atractylodes lancea volatile oils promoting MSCs to chondrogenic differentiation. It may provide an alternative strategy for treatment purposes and diagnosis in clinic.

scholarly journals Performance of different levels of nitrogen and phosphorus on herbage yield, nutrient content and uptake of rainfed palmarosa (Cymbopogan martini var. Motia) - A Review

2018 ◽  
Author(s):  
Jayalakshmi Mitnala
Keyword(s):  
Management Practices ◽  
Nutrient Management ◽  
Skin Diseases ◽  
Nutrient Content ◽  
Volatile Oil ◽  
Raw Material ◽  
Alcoholic Beverages ◽  
Mosquito Repellent ◽  
Nitrogen And Phosphorus ◽  
Different Levels

Cymbopogan martini var. Motia commonly known as Palmarosa or Rosha grass is a tall perennial tufted hedge native of most parts of sub-tropical India. Palmarosa oil has a sweet floral rose-like odour also has notes of rye bread, tea and clary sage. The oil is extensively used as perfumery raw material in soaps; floral rose-like perfumes; cosmetics preparations and in the manufacture of mosquito repellent products. It is used for flavoring tobacco products, foods and non-alcoholic beverages. The volatile oil is used as a remedy for lumbago, stiff joints, skin diseases and for bilious complaints.Considering the importance of this crop every efforts are being made to increase the oil production of the crop by using improved nutrient management practices.

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