Area under Absorbance Curve Vs. Concentration for UV-Spectrophotometric Analysis of Insulin in Different PH Conditions

Erythromycin is one of the most frequently prescribed antibiotics. With the increasing number of pharmaceutical industries, the possibility of purchasing a substandard product is on the high side. This makes it necessary to subject various commercially produced brands of erythromycin to quality evaluation in order to ascertain the quality. In this quality assessment study, six (6) brands of erythromycin tablets were subjected to physicochemical assay using standard physical methods and UV/VIS spectrophotometric analysis at 480 nm. The calibration curve for reference erythromycin was linear over a concentration range of 10-100 μg/ml with a correlation coefficient (R2) of 0.999. All the brands had standard deviation of weight greater than ±5 for the weight uniformity test. 66.67% of the brands passed the test for content of active ingredient. 83.33% of the brands examined passed the hardness test. All the brands failed the friability test because the percentage weight loss are greater than 1% as specified in the official book. Keywords: Erythromycin stearate; Tablets; Brands; Pharmaceutical Industries

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UV SPECTROPHOTOMETRIC ANALYSIS AND VALIDATION OF OSELTAMIVIRPHOSPHATE IN PURE AND PHARMACEUTICAL FORMULATION

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i2.37505 ◽

2020 ◽

pp. 111-114 ◽

Cited By ~ 1

Author(s):

SACHIN A. YANJANE ◽

SHRISHAIL M. GHURGHURE ◽

VINOD K. MATOLE

Keyword(s):

Spectrophotometric Method ◽

Spectrophotometric Analysis ◽

Pharmaceutical Formulation ◽

Ich Guidelines ◽

Highly Sensitive ◽

Double Beam ◽

Recovery Study ◽

Uv Visible ◽

Oseltamivir Phosphate ◽

Simple Economic

Objective: A new, simple, economical, precise, sensitive, linear, accurate, rapid UV spectrophotometric method has been developed for the estimation of Oseltamivir Phosphate in pure form and pharmaceutical formulation. Methods: This UV method was developed using Methanol as a solvent. In the present method, the wavelength selected for analysis was 218 nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 10-50µg/ml. Accuracy was performed by using a recovery study. The amount of drug recovered was found to be in the range of 99.01-100.1%. The % RSD value was found to be less than 2. Conclusion: The developed UV spectrophotometric method was found to be simple, economic, sensitive, easy, accurate, linear, specific and highly sensitive and can be used for routine estimation of Oseltamivir Phosphate.

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Adsorption of p-Arsanilic Acid on Iron (Hydr)oxides and Its Implications for Contamination in Soils

Minerals ◽

10.3390/min11020105 ◽

2021 ◽

Vol 11 (2) ◽

pp. 105

Author(s):

Yifan Yang ◽

Shiyong Tao ◽

Zhichun Dong ◽

Jing Xu ◽

Xiang Zhang ◽

...

Keyword(s):

Iron Oxides ◽

Organic Matter Content ◽

Feed Additives ◽

Unit Surface Area ◽

Potential Hazard ◽

Order Kinetic ◽

Positive Correlation ◽

Arsanilic Acid ◽

Organic Arsenic ◽

Ph Conditions

Because of the diversification of industries in developing cities, the phenomenon of the simultaneous contamination of various kinds of pollutants is becoming common, and the environmental process of pollutants in multi-contaminated environmental mediums has attracted attention in recent years. In this study, p-arsanilic acid (ASA), a kind of organic arsenic feed additive that contains the arsenic group in a chemical structure, is used as a typical contaminant to investigate its adsorption on iron oxides and its implication for contaminated soils. The adsorption kinetics on all solids can be fitted to the pseudo-second-order kinetic model well. At the same mass dosage conditions, the adsorption amount per unit surface area on iron oxides follows the order α-FeOOH > γ-Fe2O3 > α-Fe2O3, which is significantly higher than that for actual soil, because of the lower content of iron oxides in actual soil. Lower pH conditions favor ASA adsorption, while higher pH conditions inhibit its adsorption as a result of the electrostatic repulsion and weakened hydrophobic interaction. The presence of phosphate also inhibits ASA adsorption because of the competitive effect. Correlations between the amount of ASA adsorption in actual soil and the Fe2O3 content, total phosphorus content, arsenic content, and organic matter content of actual soil are also investigated in this work, and a moderate positive correlation (R2 = 0.630), strong negative correlation (R2 = 0.734), insignificant positive correlation (R2 = 0.099), and no correlation (R2 = 0.006) are found, respectively. These findings would help evaluate the potential hazard of the usage of organic arsenic feed additives, as well as further the understanding of the geochemical processes of contaminants in complicated mediums.

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Emulsion Formation and Stabilizing Properties of Olive Oil Cake Crude Extracts

Processes ◽

10.3390/pr9040633 ◽

2021 ◽

Vol 9 (4) ◽

pp. 633

Author(s):

Firdaous Fainassi ◽

Noamane Taarji ◽

Fatiha Benkhalti ◽

Abdellatif Hafidi ◽

Marcos A. Neves ◽

...

Keyword(s):

Olive Oil ◽

Ethanol Extract ◽

Active Components ◽

Oil In Water ◽

Surface Active ◽

Ph Conditions ◽

Oil Water ◽

Surface Active Compounds ◽

Ionizable Groups ◽

Emulsifying Ability

The surface-active and emulsifying properties of crude aqueous ethanolic extracts from untreated olive oil cake (OOC) were investigated. OOC extracts contained important concentrations of surface-active components including proteins, saponins and polyphenols (1.2–2.8%, 7.8–9.5% and 0.7–4.5% (w/w), respectively) and reduced the interfacial tension by up to 46% (14.0 ± 0.2 mN m−1) at the oil–water interface. The emulsifying ability of OOC extracts was not correlated, however, with their interfacial activity or surface-active composition. Eighty percent aqueous ethanol extract produced the most stable oil-in-water (O/W) emulsions by high-pressure homogenization. The emulsions had average volume mean droplet diameters of approximately 0.4 µm and negative ζ-potentials of about -45 mV, and were stable for up to 1 month of storage at 5, 25 and 50 °C. They were sensitive, however, to acidic pH conditions (<5) and NaCl addition (≥25 mM), indicating that the main stabilization mechanism is electrostatic due to the presence of surface-active compounds with ionizable groups, such as saponins.

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Effects of plastic mulch film residues on soil-microbe-plant systems under different soil pH conditions

Chemosphere ◽

10.1016/j.chemosphere.2020.128901 ◽

2020 ◽

pp. 128901

Author(s):

Yin Liu ◽

Qing Huang ◽

Wen Hu ◽

Jiemin Qin ◽

Yingrui Zheng ◽

...

Keyword(s):

Soil Ph ◽

Plastic Mulch ◽

Soil Microbe ◽

Mulch Film ◽

Ph Conditions

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Evaluation of the physicochemical properties of pectin extracted from Musa paradisiaca banana peels at different pH conditions in the formation of nanoparticles

Heliyon ◽

10.1016/j.heliyon.2021.e06059 ◽

2021 ◽

Vol 7 (1) ◽

pp. e06059

Author(s):

David Arias ◽

Johny Rodríguez ◽

Betty López ◽

Paula Méndez

Keyword(s):

Physicochemical Properties ◽

Musa Paradisiaca ◽

Ph Conditions

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Plasma thiol/disulphide homeostasis changes in patients with restless legs syndrome

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2020-1659 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Ertan Kucuksayan ◽

Serkan Ozben ◽

Selma Topaloglu Tuac ◽

Mesrure Koseoglu ◽

Ozcan Erel ◽

...

Keyword(s):

Oxidative Stress ◽

Restless Legs Syndrome ◽

Rating Scales ◽

Spectrophotometric Analysis ◽

Healthy Controls ◽

Restless Legs ◽

Severity Rating ◽

Total Thiol ◽

Disease Rating ◽

Positive Correlations

Abstract Objectives Restless legs syndrome (RLS) is a common neurological condition. Oxidative stress plays an important role in its pathogenesis. Thiol-disulphide homeostasis (TDH) is a new biomarker of oxidative stress. We studied plasma TDH to determine whether TDH could be used as a new biomarker for RLS and evaluated correlations between TDH and various disease severity rating scales. Methods A total of 25 RLS patients and 25 healthy controls were included into the study. TDH status was determined using an automated spectrophotometric analysis method and correlations were analyzed between the TDH status and various disease rating scales in the RLS patients. Results Plasma total (401 ± 27 μmol/L) and native thiol (354 ± 30 μmol/L) levels were significantly lower, but disulphide level (24 ± 6 μmol/L) was significantly (<0.0001) higher in the RLS patients compared to the controls (455 ± 36, 424 ± 37, 15 ± 5 μmol/L, respectively). The disulphide/native thiol and disulphide/total thiol ratios increased, in contrast, native thiol/total thiol ratio decreased significantly in the RLS patients compared to the healthy controls (<0.0001). The disulphide levels correlated positively with age and various rating scores of the RLS patients. International Restless Legs Syndrome Study Group (IRLSSG) rating score and age correlated negatively with the total and native thiol levels. Conclusions Our findings indicate increased oxidative stress in the RLS patients reflected by decreased native and total thiol, and increased disulphide levels and positive correlations between the disulphide levels and various rating scores. We suggest dynamic TDH status to be used as a novel biomarker for the diagnosis and follow-up of the RLS patients.

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Assessment of Mini-Tablets Coating Uniformity as a Function of Fluid Bed Coater Inlet Conditions

Pharmaceutics ◽

10.3390/pharmaceutics13050746 ◽

2021 ◽

Vol 13 (5) ◽

pp. 746

Author(s):

Magdalena Turk ◽

Rok Šibanc ◽

Rok Dreu ◽

Maja Frankiewicz ◽

Małgorzata Sznitowska

Keyword(s):

Coating Layer ◽

Spectrophotometric Analysis ◽

Material Distribution ◽

Spray Chamber ◽

Colorimetric Analysis ◽

Swirl Generator ◽

Coating Uniformity ◽

Fluid Bed ◽

Inlet Conditions

This study concerned the quality of mini-tablets’ coating uniformity obtained by either the bottom spray chamber with a classical Wurster distributor (CW) or a swirl distributor (SW). Mini-tablets with a diameter of 2.0, 2.5, and 3.0 mm were coated with hypromellose using two different inlet air distributors as well as inlet airflow rates (130 and 156 m3/h). Tartrazine was used as a colorant in the coating layer and the coating uniformity was assessed by spectrophotometric analysis of solutions obtained after disintegration of the mini-tablets (n = 100). Higher uniformity of coating material distribution among the mini-tablets was observed in the case of SW distributor, even for the biggest mini-tablets (d = 3.0 mm), with an RSD no larger than 5.0%. Additionally, coating thickness was evaluated by colorimetric analysis (n = 1000), using a scanner method, and expressed as a hue value. A high correlation (R = 0.993) between inter-tablet variability of hue and UV-Vis results was obtained. Mini-tablets were successfully coated in a fluid bed system using both a classical Wurster distributor as well as a swirl generator. However, regardless of the mini-tablets’ diameter, better film uniformity was achieved in the case of a distributor with a swirl generator.

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