Fungitoxic effect of inorganic salts for the management of seed borne Macrophomina phaseolina and Fusarium sp. causing charcoal rot and wilt disease in blackgram

The laboratory experiments were carried out to study the effect of different salts viz., potassium chloride (KCl), potassium phosphate dibasic (K2HPO4), sodium carbonate (Na2CO3), sodium bicarbonate (NaHCO3) and ammonium molybdate (NH4)6 Mo7O24) on seed borne M. phaseolina and Fusarium sp. as an alternative to synthetic fungicides for the control of charcoal rot and wilt diseases in blackgram. The evaluation of different salts was performed in vitro using various concentrations viz., 0.5, 1.0, 2.0, 4.0 and 8.0 per cent (w/v). Among the salts tested against M. phaseolina, sodium carbonate, sodium bicarbonate and ammonium molybdate at 0.5, 2.0 and 4.0 per cent respectively inhibited the fungal growth. Among the salts tested against Fusarium sp. sodium carbonate (Na2CO3) at 4.0 per cent concentration recorded complete inhibition of the mycelial growth compared to the other salts. Also seed priming of these salts significantly reduced the seed borne infection due to M. phaseolina and Fusarium sp. under standard blotter test. The salts like potassium chloride and potassium phosphate dibasic (K2HPO4) at all concentrations did not inhibit M. phaseolina and Fusarium sp. which recorded 100 per cent mycelial growth as that of control.

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Saprophytic microorganisms with potential for biological control of Botrytis cinerea on Geraldton waxflower flowers

Australian Journal of Experimental Agriculture ◽

10.1071/ea00112 ◽

2001 ◽

Vol 41 (5) ◽

pp. 697 ◽

Cited By ~ 8

Author(s):

D. R. Beasley ◽

D. C. Joyce ◽

L. M. Coates ◽

A. H. Wearing

Keyword(s):

Botrytis Cinerea ◽

Mycelial Growth ◽

Germ Tube ◽

Conidial Germination ◽

Pseudomonas Sp ◽

Trichoderma Spp ◽

Fusarium Sp ◽

Flower Abscission ◽

Germ Tube Elongation

Saprophytic bacteria, yeasts and filamentous fungi were isolated from Geraldton waxflower flowers and screened to identify potential antagonism towards Botrytis cinerea. Isolates from other sources (e.g. avocado) were also tested. Isolates were initially screened in vitro for inhibition of B. cinerea conidial germination, germ tube elongation and mycelial growth. The most antagonistic bacteria, yeasts and fungi were selected for further testing on detached waxflower flowers. Conidia of the pathogen were mixed with conidia or cells of the selected antagonists, co-inoculated onto waxflower flowers, and the flowers were sealed in glass jars and incubated at 20˚C. The number of days required for the pathogen to cause flower abscission was determined. The most antagonistic bacterial isolate, Pseudomonas sp. 677, significantly reduced conidial germination and retarded germ tube elongation of B. cinerea. None of the yeast or fungal isolates tested was found to significantly reduce conidial germination or retard germ tube elongation, but several significantly inhibited growth of B. cinerea. Fusarium sp., Epicoccum sp. and Trichoderma spp. were the most antagonistic of these isolates. Of the isolates tested on waxflower, Pseudomonas sp. 677 was highly antagonistic towards B. cinerea and delayed waxflower abscission by about 3 days. Trichoderma harzianum also significantly delayed flower abscission. However, as with most of the fungal antagonists used, inoculation of waxflower flowers with this isolate resulted in unsightly mycelial growth.

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Evaluation of different salts for the control of lettuce varnish spot: when phytotoxicity rules

Canadian Journal of Plant Science ◽

10.1139/cjps-2017-0237 ◽

2018 ◽

Vol 98 (3) ◽

pp. 753-761 ◽

Cited By ~ 4

Author(s):

Maxime Delisle-Houde ◽

Vicky Toussaint ◽

Hicham Affia ◽

Russell J. Tweddell

Keyword(s):

Sodium Bicarbonate ◽

Sodium Carbonate ◽

Sodium Benzoate ◽

Leaf Tissue ◽

In Vitro Assays ◽

Potassium Sorbate ◽

Sodium Metabisulfite ◽

Pseudomonas Cichorii ◽

Foliar Symptoms

Five generally recognised as safe (GRAS) salts with antimicrobial activity were investigated for their potential use as bactericides for the control of lettuce varnish spot [Pseudomonas cichorii (Swingle) Stapp]. The phytotoxicity of salts was first assessed using greenhouse and in vitro assays. Greenhouse assays revealed that salts showed different levels of phytotoxicity. Potassium sorbate, sodium benzoate, and sodium carbonate at higher concentrations caused a noticeable decrease of growth along with foliar phytotoxicity symptoms while sodium metabisulfite and sodium bicarbonate caused exclusively foliar symptoms. Based on the phytotoxic doses 5% determined in vitro, salts can be ranked in ascending order of phytotoxicity as follows: sodium bicarbonate, potassium sorbate, sodium carbonate, sodium benzoate, and sodium metabisulfite. When applied at concentrations causing mild to moderate foliar symptoms of phytotoxicity and no noticeable effect on growth, salts did not significantly affect (p ≤ 0.01) survival of P. cichorii on lettuce leaf tissue and did not significantly reduce (p ≤ 0.01) varnish spot severity. Although sodium metabisulfite was applied at concentrations higher than the minimum inhibitory concentration and minimum bactericidal concentration, it did not affect P. cichorii survival on leaf tissue.

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Salicylic acid in the induction of resistance to beet seedling damping-off and antifungal activity against Fusarium sp., in vitro

Semina Ciências Agrárias ◽

10.5433/1679-0359.2016v37n1p67 ◽

2016 ◽

Vol 37 (1) ◽

pp. 67 ◽

Cited By ~ 2

Author(s):

Douglas Junior Bertoncelli ◽

Sérgio Miguel Mazaro ◽

Rita De Cacia Dosciatti Serrão Rocha ◽

Nean Locatelli Dalacosta ◽

Adriano Lewandowski ◽

...

Keyword(s):

Salicylic Acid ◽

Antifungal Activity ◽

Mycelial Growth ◽

Experimental Unit ◽

Damping Off ◽

Potato Dextrose Agar Medium ◽

Seedling Length ◽

Fusarium Sp ◽

And Control

The damping off is the main disease that affects the beet crop during the seedling production. The aim of this study was to evaluate different salicylic acid (SA) concentrations for resistance induction against damping-off in beet seedling and its antifungal activity against Fusarium sp., in vitro condition. Treatment of beet seed was with SA solution by immersion during 5 minutes in the 0.5, 1.0, 1.5 and 2.0 mM concentrations and control (distilled water). It was used four replications with 20 cells by experimental unit. The experiment was carried out for 14 days in cultivate chamber with temperature (23 oC ± 2°C), lighting (12 hours photoperiod) and humidity (70% ± 10%) controlled. After this time, the germination, damping off incidence, seedling length and fresh mass matter weight were evaluated. It was evaluated also in the seedling tissue the phenylalanine ammonia-lyase (PAL), ?-1.3 glucanase and chitinase level enzymes. In the in vitro the SA was putted in PDA (potato-dextrose-agar) medium, where the Fusarium sp. mycelial growth was evaluated. The SA applied for seeds treatment didn’t had effect significant on damping off of beet seedlings, but it induced the activity of ?-1.3 glucanase enzyme, it being this higher in nine times when compared the treatment control. The SA acted in the Fusarium sp. in vitro control with fungitoxic action, suppressed mycelial growth in 28% if compared to control.

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Antimicrobial activity against Macrophomina phaseolina and the control of charcoal rot in soybeans using the homoeopathic drugs Sepia and Arsenicum album

Arquivos do Instituto Biológico ◽

10.1590/1808-1657000562016 ◽

2018 ◽

Vol 84 (0) ◽

Author(s):

Eloísa Lorenzetti ◽

José Renato Stangarlin ◽

Odair José Kuhn

Keyword(s):

Antimicrobial Activity ◽

Mycelial Growth ◽

Fungal Growth ◽

Macrophomina Phaseolina ◽

In Vitro Tests ◽

Charcoal Rot ◽

Progress Curve ◽

Homeopathic Drugs

ABSTRACT: The aim of this study was to evaluate the control of charcoal rot in soybeans using Sepia and Arsenicum album homeopathic solutions in dynamizations of 6, 12, 24, 36 and 48CH (hahnemanian centesimal scale), in addition to verify the antimicrobial activity of these treatments against Macrophomina phaseolina. Additional treatments were also used, including distilled water and a hydroalcoholic solution of 30% ethanol. In vitro tests were performed to analyze the number of micro-sclerotia and the mycelial growth of the fungus. In vivo assays were used to evaluate the progress of the disease and the size of the lesions. For the area under the mycelial growth curve (AUMGC), A. album had no effect, however, Sepia reduced up to 32% of the fungal growth. For micro-sclerotia there was no statistical difference. For the area under the disease progress curve (AUDPC), both Sepia and A. album in 24CH dynamization reduced fungal growth up to 50% and 70% in the first and second assays, respectively, compared to control treatments. These results indicate the potential of these homeopathic drugs for controlling soybean charcoal rot.

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An Attempt at Biological Control of Blossom Blight of Rose Caused by Botrytis cinerea Using some Local Trichoderma spp. Strains

Acta Phytopathologica et Entomologica Hungarica ◽

10.1556/038.55.2020.010 ◽

2020 ◽

Vol 55 (1) ◽

pp. 27-34

Author(s):

G. Zadehdabagh ◽

K. Karimi ◽

M. Rezabaigi ◽

F. Ajamgard

Keyword(s):

Botrytis Cinerea ◽

Mycelial Growth ◽

Limiting Factor ◽

Dual Culture ◽

Trichoderma Spp ◽

Khuzestan Province ◽

Inhibitory Potential ◽

Cut Rose

The northern of Khuzestan province in Iran is mainly considered as one of the major areas of miniature rose production. Blossom blight caused by Botrytis cinerea has recently become a serious limiting factor in rose production in pre and post-harvest. In current study, an attempt was made to evaluate the inhibitory potential of some local Trichoderma spp. strains against B. cinerea under in vitro and in vivo conditions. The in vitro results showed that all Trichoderma spp. strains were significantly able to reduce the mycelial growth of the pathogen in dual culture, volatile and non-volatile compounds tests compared with control, with superiority of T. atroviride Tsafi than others. Under in vivo condition, the selected strain of T. atroviride Tsafi had much better performance than T. harzianum IRAN 523C in reduction of disease severity compared with the untreated control. Overall, the findings of this study showed that the application of Trichoderma-based biocontrol agents such as T. atroviride Tsafi can be effective to protect cut rose flowers against blossom blight.

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Preparation and Evaluation of Gemifloxacin Mesylate Floating Matrix Tablets in Healthy Human Volunteers

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2017.10.1.8 ◽

2017 ◽

Vol 10 (1) ◽

pp. 3623-3630

Author(s):

Bhikshapathi D. V. R. N. ◽

Haarika B ◽

Jyothi Sri S ◽

K Abbulu

Keyword(s):

Drug Release ◽

Sodium Bicarbonate ◽

Polymer Concentration ◽

Hydroxypropyl Methylcellulose ◽

Matrix Tablets ◽

Physical Parameters ◽

Drug Bioavailability ◽

Floating Tablets

The purpose of present investigation was to develop floating matrix tablets of gemifloxacin mesylate, which after oral administration could prolong the gastric residence time, increase the drug bioavailability and diminish the side effects of irritating drugs. Tablets containing drug, various viscosity grades of hydroxypropyl methylcellulose such as HPMC K4M and HPMC K15M as matrix forming agent, Sodium bicarbonate as gas-forming agent and different additives were tested for their usefulness in formulating gastric floating tablets by direct compression method. The physical parameters, in vitro buoyancy, release characteristics and in vivo radiographic study were investigated in this study. The gemifloxacin mesylate floating tablets were prepared using HPMC K4M polymer giving more sustained drug release than the tablet containing HPMC K15M. All these formulations showed floating lag time of 30 to 47 sec and total floating time more than 12 h. The drug release was decreased when polymer concentration increases and gas generating agent decreases. Formulation that contains maximum concen-tration of both HPMC K15M and sodium bicarbonate (F9) showing sufficiently sustained with 99.2% of drug release at 12 h. The drug release from optimized formulation follows Higuchi model that indicates the diffusion controlled release. The best formulation (F9) was selected based on in vitro characteristics and used in vivo radiographic studies by incorporating barium sulphate as a radio-opaque agent and the tablet remained in the stomach for about 6 h.

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PDO Rotonda’s Red Eggplant Extract: In vitro Determination of Biological Properties and Minerals Bioaccessibility

Current Nutrition & Food Science ◽

10.2174/1573401314666180622110952 ◽

2020 ◽

Vol 16 (1) ◽

pp. 65-74

Author(s):

Ortensia Ilaria Parisi ◽

Mariarosa Ruffo ◽

Fabio Amone ◽

Rocco Malivindi ◽

Domenico Gorgoglione ◽

...

Keyword(s):

Control Sample ◽

Specific Area ◽

Biological Properties ◽

Ammonium Molybdate ◽

Antihypertensive Activity ◽

Protected Designation Of Origin ◽

The Family ◽

Oil Red O Staining

Background: The Rotonda’s Red Eggplant belongs to the family of Solanum aethiopicum and it is cultivated in a specific area of Potenza (Basilicata, South of Italy) including villages of Rotonda, Viggianello, Castelluccio Superiore and Castelluccio Inferiore. The Red Eggplant cultivated in this area has gained the PDO, “Protected Designation of Origin”. Objective: The aim of this research was to evaluate the use of PDO Rotonda’s Red Eggplant extract as a possible nutraceutical supplement. The antioxidant, antihypertensive, hypoglycemic, and hypolipidemic properties were in vitro evaluated. Methods: The antioxidant activity was investigated by evaluating the scavenging properties against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals and by performing the Ammonium Molybdate and Folin-Ciocalteu assay. The hypoglycemic and antihypertensive activity was studied by evaluating the α-Amylase, α-Glucosidase and Angiotensin Converting Enzyme, respectively, inhibiting activity. In order to evaluate the hypolipidemic activity, the pancreatic lipase inhibiting property was determined and Oil Red O staining assay was performed. Finally, to evaluate the possible use of this extract as a minerals supplement, Selenium, Potassium and Chrome bioaccessibility was studied. Results: The obtained results underline the good antioxidant, hypoglycemic, antihypertensive and hypolipidemic in vitro properties of the PDO Rotonda’s Red Eggplant extract. Moreover, the obtained data show a higher minerals bioaccessibility and this higher value could be ascribable to the natural phytocomplex of PDO Rotonda’s Red Eggplant, which increases the minerals bioaccessibility if compare it with a control sample. Conclusion: The obtained results show that PDO Rotonda’s Red Eggplant extract, might be used as a possible nutraceutical supplement, along with traditional therapies, both for its biological properties and for its minerals bioaccessibility value.

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Management of collar rot disease in chickpea by Trichoderma species

Journal of AgriSearch ◽

10.21921/jas.v7i03.18694 ◽

2020 ◽

Vol 7 (03) ◽

Author(s):

PREM PANDEY ◽

G. C. SAGAR ◽

SUNDARMAN SHRESTHA2 ◽

HIRAKAJI MANANDHAR ◽

RITESH K. YADAV ◽

...

Keyword(s):

Growth Inhibition ◽

Mycelial Growth ◽

Sclerotium Rolfsii ◽

Maximum Growth ◽

Pot Experiment ◽

Trichoderma Spp ◽

Trichoderma Species ◽

Collar Rot ◽

Rot Disease

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).

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Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes

Antibiotics ◽

10.3390/antibiotics10060664 ◽

2021 ◽

Vol 10 (6) ◽

pp. 664

Author(s):

Matías Olivera ◽

Ninoska Delgado ◽

Fabiola Cádiz ◽

Natalia Riquelme ◽

Iván Montenegro ◽

...

Keyword(s):

Mycelial Growth ◽

Inhibitory Effect ◽

Biocontrol Agents ◽

Environmental Cost ◽

Antifungal Compounds ◽

Table Grapes ◽

Dual Cultures ◽

Bunch Rot ◽

Direct Bioautography

Gray and summer bunch rot are important diseases of table grapes due to the high economic and environmental cost of their control with synthetic fungicides. The ability to produce antifungal compounds against the causal agents Botrytis, Aspergillus, Penicillium, and Rhizopus of two microorganisms isolated from table grapes and identified as Hanseniaspora osmophila and Gluconobacter cerinus was evaluated. In dual cultures, both biocontrol agents (together and separately) inhibited in vitro mycelial growth of these pathogens. To identify the compounds responsible for the inhibitory effect, extractions were carried out with organic solvents from biocontrol agents separately. Through dual cultures with pathogens and pure extracts, only the hexane extract from H. osmophila showed an inhibitory effect against Botrytis cinerea. To further identify these compounds, the direct bioautography technique was used. This technique made it possible to determine the band displaying antifungal activity at Rf = 0.05–0.2. The compounds present in this band were identified by GC-MS and compared to the NIST library. The most abundant compounds, not previously reported, corresponded to alkanes, ketones, alcohols, and terpenoids. H. osmophila and G. cerinus have the potential to control the causal agents of gray and summer bunch rot of table grapes.

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