Effect of inclusion of graded level of water hyacinth on in vitro gas production kinetics and chemical composition of alfalfa hay based beef cattle diets

2016 ◽  
Author(s):  
Gerardo Pamanes-Carrasco ◽  
Manuel Murillo-Ortiz ◽  
Esperanza Herrera-Torres ◽  
Agustin Corral-Luna
Keyword(s):  
Chemical Composition ◽  
Beef Cattle ◽  
Methane Production ◽  
Water Hyacinth ◽  
Gas Production ◽  
Co2 Production ◽  
Total Phenolic ◽  
Promising Alternative ◽  
Alfalfa Hay

The aim of this study was to evaluate the inclusion of water hyacinth (WH) as a possible substitution of alfalfa hay (AH) in diets of beef cattle on in vitro methane production, gas kinetics and chemical composition. AH in the diets was substituted by WH at 0% (T1, as a control), 25% (T2), 50% (T3), 75% (T4) and 100% (T5). Methane, CO2 and gas production parameters were recorded after 24 and 48h of incubation. NDF, condensed tannins and total phenolic compounds increased when AH was substituted. Likewise, the net gas production decreased linearly when AH was substituted. However, gas production rate among treatments were similar. Methane and CO2 production decreased linearly with inclusion of WH. These results indicate that WH arises as a promising alternative for mitigating methane production in ruminants.

scholarly journals Reduction in the in vitro ruminal methane production with the inclusion of Cucurbita foetidissima

2020 ◽  
Vol 26 (1) ◽  
pp. e1941
Author(s):  
Gerardo Pámanes-Carrasco ◽  
Manuel Murillo-Ortiz ◽  
Damián Reyes-Jáquez ◽  
Roberto Valencia ◽  
Esperanza Herrera Torres
Keyword(s):  
Beef Cattle ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Latency Period ◽  
Gas Production ◽  
Co2 Production ◽  
Alfalfa Hay ◽  
Cattle Feeding ◽  
Experimental Treatments

Objective.This research aimed to evaluate the inclusion of Cucurbita foetidissima (BG) leaves as a partial or total substitution of alfalfa hay (AH) in beef cattle diets on in vitro methane output, gas kinetics and volatile fatty acids production. Materials and Methods.Five experimental treatments were formulated with the inclusion of BG as alfalfa hay (AH) substitute at 0, 25, 50, 75 and 100% in experimental treatments denominated CON (control), BG25, BG50, BG75 and BG100, respectively. Results.Lignin and organic matter decreased with BG inclusion (p<0.05). However, NDF, CT and TPC increased with higher levels of BG (p<0.05). Maximum gas production diminished with BG inclusion (p<0.05); whereas, microbial protein production, specific gas production rate and latency period were not affected (p>0.05). Methane production decreased linearly with BG inclusion (p<0.05). Nevertheless, CO2 production showed no changes with inclusion of graded levels of BG in the experimental treatments (p>0.05). Additionally, acetate and butyrate were not affected by BG inclusion (p>0.05); although, propionate increased linearly among treatments (p<0.05). Furthermore, inclusion of 75% of BG reduced 31% in vitro methane production without changes in CO2 production, suggesting an inhibition of endogenous methanogenesis. Conclusions.These results promote the inclusion of BG leaves as a potential and environmentally friendly forage source for beef cattle feeding.

scholarly journals Silage of Intercropping Corn, Palisade Grass, and Pigeon Pea Increases Protein Content and Reduces In Vitro Methane Production

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1784
Author(s):  
Beatriz Ligoski ◽  
Lucas Ferreira Gonçalves ◽  
Flavio Lopes Claudio ◽  
Estenio Moreira Alves ◽  
Ana Maria Krüger ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Methane Production ◽  
Nutritional Quality ◽  
Production Systems ◽  
Animal Feed ◽  
Lignin Content ◽  
Pigeon Pea ◽  
Gas Production ◽  
In Vitro Gas Production

Legume–grass intercropping systems are a sustainable option to improve nutritional quality of animal feed and decrease livestock greenhouse gas emissions. Thus, the present study evaluated yield, chemical composition and in vitro gas production of silages produced with intercropped palisade grass (Urochloa brizantha.(A.Rich.) R.D.Webster), pigeon pea (Cajanus cajan cv. Super N) and corn (Zea mays. L.). Forage was harvested and placed inside micro-silos, which were opened after 100 days and samples were collected for chemical composition and in vitro gas production analyses. Intercropped silage had higher crude protein, acid detergent fiber, and lignin content than corn silage. Moreover, intercropped silage decreased total gas and methane production. Therefore, intercropped silage showed potential to increase conserved feed nutritional quality and reduce methane emissions in livestock production systems.

PSXIV-1 In vitro evaluation of methane mitigation using monensin and ammonium nitrate in beef cattle diets

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 471-472
Author(s):  
Ana Paula Tarozo ◽  
Annelise Aila G Gomes Lobo ◽  
Yuli Andrea A Peña Bermudez ◽  
Danny Alexander Rojas Moreno ◽  
Rafaela Zuliani Spalato ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Ammonium Nitrate ◽  
Methane Production ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Feed Additives ◽  
Control Treatment ◽  
Fermentation Kinetics ◽  
Fermentation Parameters

Abstract Currently, the use of feed additives appears as an alternative in reducing the environmental impact of animal agriculture, reducing the emission of greenhouse gases and increasing the acceptability of exports in international trade. Thus, the objective of the present study was to evaluate the in vitro rumen fermentation parameters by adding 4.5% ammonium nitrate and 30 ppm of the additive sodium monensin to beef cattle diets, searching for the best alternative to mitigate methane production. The experiment was performed in an in vitro gas production system, and the fermentation kinetics, methanogenesis and short-chain fatty acid (SCFA) production were studied. Regarding methanogenesis, it was observed that the diet with ammonium nitrate showed higher in vitro degradability in DM (P = 0.017) and lower methane production (in ml/g of DM; P = 0.0088), compared to the diet with sodium monensin. Considering the fermentation kinetics, it can be stated that acetate production in molar (%) was lower in control and monensin diets, and higher in nitrate and nitrate + monensin diets (P &lt; 0.0001). It is concluded that both treatments ammonium nitrate + sodium monensin and ammonium nitrate alone have mitigating effect on methane emission, when compared to the control treatment. However, ammonium nitrate is more effective in this regard, producing less methane in vitro and having no negative effect on rumen fermentation parameters.

scholarly journals PSII-5 Chemical composition, in vitro dry matter digestibility, gas production and methane emission of maintenance, growth and production diets/rations of buffaloes

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 408-408
Author(s):  
Uchenna Anele ◽  
Sultan Singh ◽  
B P Kushwaha ◽  
P K Gupta ◽  
S Bhattacharya
Keyword(s):  
Chemical Composition ◽  
Methane Production ◽  
Dry Matter ◽  
Chemical Constituents ◽  
Gas Production ◽  
Protein Fractions ◽  
Mean Values ◽  
Dry Matter Digestibility ◽  
Structural Carbohydrate

Abstract Ten types of diets were prepared to meet the nutrient requirements of buffaloes for maintenance (MD1 to MD10), growth (GD1 to GD10) and production/lactation (PD1 to PD10). A total of 30 diets were evaluated for chemical composition, in vitro gas and methane production, and dry matter (DM) disappearance using buffalo rumen liquor. The CNCPS was used to estimate the carbohydrate and protein fractions. Protein fractions PB1 and PB2 of maintenance diets were lower (P &lt; 0.05) than growth and production diets, while protein fractions (PB3) and Pc were (P &lt; 0.05) higher in maintenance than in growth and production diets. Mean values of PA was (P &lt; 0.05) higher in growth diets (136.9) than production (114.8) and maintenance diets (105.6 g/kg DM). Maintenance diets had (P &lt; 0.05) higher structural carbohydrate contents (586.2 g/kg DM) than production diets (513.0 g/kg DM). Carbohydrate fraction (CB1) was highest (P &lt; 0.05) in production diets (187.2 g/kg DM) followed by growth (129.5 g/kg DM) and maintenance diets (96.1 g/kg DM). In vitro gas production at different time periods (12, 24 and 48 h) was similar for maintenance (63.04, 51.98 and 48.15 ml/g DM), growth (63.83, 52.73 and 48.250) and production diets (63.51, 52.54 and 47.21 ml/g DM). Cumulative methane production was numerically lower for maintenance (28.40 ml/g DM) than growth (29.58 ml/g DM) and production diets (33.13 ml/g DM). In vitro methane production as a proportion of degraded DM (ml/g DDM and g/kg DDM) was similar for maintenance (14.21 and 29.53), growth (42.19 and 30.25) and production diets (41.26 and 29.58). Dietary chemical constituents such as EE, lignin, NDIN, ADIN and PB3 and Cc were (P &lt; 0.05) negatively associated with methane production, while OM, NPN, SP, PA and PB1, TCHO and CB2 were positively (P &lt; 0.05) correlated with methane production.

The prediction of in vivo methane production and animal performance from the in vitro gas production technique

2003 ◽  
Vol 2003 ◽  
pp. 157-157
Author(s):  
A. Bortolozzo ◽  
D. K. Lovett ◽  
S. Lovell ◽  
L. Stack ◽  
F. P. O’Mara
Keyword(s):  
Chemical Composition ◽  
Methane Production ◽  
Gas Production ◽  
Animal Performance ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Ad Libitum

The in vivo determination of methane (CH4) production requires specialist equipment which is costly to maintain. Whilst the in vitro gas production technique has been demonstrated to show potential to rank diets for their methanongenic potential at maintenance planes of nutrition (Moss and Givens, 1997) no study has investigated this relationship when feedstuffs are fed ad libitum. The objective of this study was to assess the ability of the technique to predict in vivo CH4 production and animal performance from six diets differing in their chemical composition.

Chemical properties, antioxidant activities and in vitro fermentation profiles of some shrubs of North Western Tunisia

2018 ◽  
Author(s):  
H. Selmi ◽  
M. Hasnaoui ◽  
A. Bahri ◽  
C. Abbes ◽  
S. Dallali ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antioxidant Activities ◽  
Chemical Properties ◽  
Gas Production ◽  
Total Phenolic ◽  
Myrtus Communis ◽  
In Vitro Fermentation ◽  
In Vitro Gas Production

The main aims of this study were to analyze the chemical composition and total phenolic, and to evaluate the in vitro fermentation and antioxidant activities from some shrubs (Erica arborea, Myrtus communis, Arbutus unedo and Phillyrea latifolia). The determination of the chemical composition, total phenolic, flavonoid contents and the antioxidant activity were investigated by using in vitro methods. The in vitro gas production and ruminal parameters were determined in the presence of goat rumen juice. Total nitrogen content was significantly high with Myrtus communis (p less than 0.01). Total phenolic and flavonoid content vary by species and season (p less than 0.01). It can be concluded that all parameters were significantly affected by the seasonal period and the shrubs nature. Expect the OM that was similar regardless the seasonal period.

scholarly journals PSXI-17 Influence of hops on in vitro ruminal fermentation of corn grain

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 408-408
Author(s):  
Yamicela Castillo-Castillo ◽  
Raul Solis ◽  
Armando A Quintana ◽  
Claudio Arzola ◽  
Ana Luisa Olivas-Palacios ◽  
...  
Keyword(s):  
Methane Production ◽  
Beef Cows ◽  
Gas Production ◽  
Co2 Production ◽  
Ruminal Fermentation ◽  
Ruminal Fluid ◽  
Fermentation Parameters ◽  
Corn Grain

Abstract An in vitro incubation was carried out to evaluate the potential of hops (Humulus lupulus) as an alternative to antibiotics for upgrading animal production. Whole pellets of hops (Variety Galena) were ground and incubated in a batch culture of ruminal fluid (2000 mg of ground corn grain + 10 mL of fresh rumen liquor). Ruminal fluid was collected from two beef cows through an esophageal tube. The hops were incubated by 24 h at levels of 0, 800, 1600 and 2400 µg/mL. Data were analyzed statistically by analysis of variance using PROC GLM of SAS. Hops addition linearly decreased (P &lt; 0.01) gas production (GP; 90.89, 61.73, 36.63 and 28.37 µmol/g respectively) and methane production (MP; 9.76, 1.70, 1.30 and 0.46 µmol/g respectively). The CO2 production linearly increased as levels of hops increased (P &lt; 0.02; 87.5, 88.4, 98.1 and 99.3 µmol/g respectively). The ammonia-N production was reduced in treatment 2 with respect to other treatments (P &lt; 0.03; 12.6, 9.2, 13.7 and 13.5 µmol/g). Effects on ruminal fermentation of corn grain were dose dependent of hops. Addition of hops in ruminant feeding may offer a means to decrease ruminal methane production. Further research is needed to test efficacy of hops on other in vivo rumen-fermentation parameters.

Effect of Alfalfa Hay Substitution by Raw Garlic Leaves on In vitro Gas Methane Production and Ruminal Fermentation

2021 ◽  
Author(s):  
Karla Torres-Fraga ◽  
Manuel Murillo-Ortiz ◽  
Esperanza Herrera-Torres ◽  
Gerardo Pámanes-Carrasco ◽  
Jesús Páez-Lerma ◽  
...  
Keyword(s):  
Microbial Biomass ◽  
Methane Production ◽  
Ammonia Nitrogen ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Fractional Rate ◽  
Alfalfa Hay ◽  
Fermentation Pattern ◽  
In Vitro Rumen Fermentation

Background: The aim of present research was to evaluate under in vitro conditions, the effect of alfalfa hay substitution by raw garlic leaves on ruminal fermentation patterns and methane production in diets ruminants. Methods: Four treatments were evaluated: (T1) alfalfa hay (50%); (T2) alfalfa hay (33%) + raw garlic leaves (17%); (T3) alfalfa hay (17%) + raw garlic leaves (33%) and (T4) raw garlic leaves (50%). Result: The highest values of fractional rate of gas production (kd), ammonia-nitrogen (NH3-N), propionate and microbial biomass synthesis (MBS) was recorded in T4 and the lowest in T1 (P less than 0.05). In contrast, the highest methane production was recorded in T1 and the lowest in T4 (P less than 0.05). It was concluded that the substitution of alfalfa hay by raw garlic leaves in diet with 50% roughages and 50% concentrate result in an improvement in vitro rumen fermentation pattern and decreases the methane production.

Chemical composition, in vitro gas production, methane production and fatty acid profile of canola silage (Brassica napus) with four levels of molasses

2019 ◽  
Vol 51 (6) ◽  
pp. 1579-1584
Author(s):  
Daniel Limón-Hernández ◽  
Adolfo Armando Rayas-Amor ◽  
Anastacio García-Martínez ◽  
Julieta Gertrudis Estrada-Flores ◽  
Mayra Núñez López ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Brassica Napus ◽  
Chemical Composition ◽  
Fatty Acid Profile ◽  
Methane Production ◽  
Gas Production ◽  
In Vitro Gas Production ◽  
Four Levels

scholarly journals The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 786 ◽  
Author(s):  
Florencia Garcia ◽  
Darío Colombatto ◽  
M. Alejandra Brunetti ◽  
M. José Martínez ◽  
M. Valeria Moreno ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Methane Production ◽  
Gas Production ◽  
Feed Additives ◽  
Slight Reduction ◽  
Ruminal Fermentation ◽  
Oxygenated Compounds ◽  
In Vitro Gas Production ◽  
Different Characteristics

There is interest in identifying natural products capable of manipulating rumen microbial activity to develop new feed additives for ruminant nutrition as a strategy to reduce methane. Two trials were performed using the in vitro gas production technique to evaluate the interaction of substrate (n = 5) and additive (n = 6, increasing doses: 0, 0.3, 3, 30, and 300 µL/L of essential oils—EO—of Lippia turbinata or Tagetes minuta, and monensin at 1.87 mg/L). The two EO utilized were selected because they differ markedly in their chemical composition, especially in the proportion of oxygenated compounds. For both EO, the interaction between the substrate and additive was significant for all variables; however, the interaction behaved differently for the two EO. Within each substrate, the response was dose-dependent, without effects at a low level of EO and a negative outcome at the highest dose. The intermediate dose (30 µL/L) inhibited methane with a slight reduction on substrate digestibility, with L. turbinata being more effective than T. minuta. It is concluded that the effectiveness of the EO to reduce methane production depends on interactions between the substrate that is fermented and the additive dose that generates different characteristics within the incubation medium (e.g., pH); and thus, the chemical nature of the compounds of the EO modulates the magnitude of this response.

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