Gene LPL, STAT5 and AGPAT6 Polymorphisms Relationship with Goat Milk Traits

Author(s):  
B. Šlyžienë ◽  
L. Anskienë ◽  
E. Šlyžius ◽  
R. Bižienë ◽  
V. Juozaitienë

Background: Several single nucleotide polymorphisms had been detected in goats and for the researchers it is important to reveal candidate genes with substantial effects on the traits of economic importance. The aim of this study was to investigate the association between LPL, STAT5 and AGPAT6 gene polymorphisms and milk traits of goats. Methods: We investigated about 204 goats, four different breeds (Czech White Shorthaired, Saanen, Anglo Nubian, Alpine) and two crossbreeds (Saanen and Alpine, Saanen and Anglo Nubian). The milk samples were analysed using spectrophotometers LactoScope 550 and LactoScope FTIR (Delta Instruments, the Netherlands). The somatic cell count (SCC) in milk was determined by flow cytometry method using the Somascope CA-3A4 (Delta Instruments, the Netherlands). Variations of the goat AGPAT6, STAT5, LPL genes were detected by PCR-RFLP method. Result: Analysis of AGPAT6 gene revealed that goats with GC genotype had higher milk yield, fat content and lactose content; goats with CG genotype had higher protein content. STAT5 gene analysis showed that goats with CT genotype had higher milk yield; goats with CC genotype had higher fat content and lactose content; goats with TT genotype had higher protein and urea content and also SCC. According to LPL gene analysis, goats with CC genotype had higher milk yield while goats with GG genotype had higher fat and protein content. The highest milk yield was estimated in Saanen and Alpine crossbreed goats while the highest milk fat and protein content was estimated in Anglo Nubian goats.

2004 ◽  
Vol 47 (3) ◽  
pp. 275-285
Author(s):  
D. Bömkes ◽  
H. Hamann ◽  
O. Distl

Abstract. Title of the paper: Influence of systematic environmental effects on milk performance traits in German Improved Fawn The objectives of this study were to analyse the influence of fixed effects on milk traits of German Improved Fawn. The analysis was based on 27,778 test day records of 1,848 German Improved Fawn with 3,574 lactation records. The milk records were sampled between 1988 and 2002 from 229 flocks in Lower Saxony, Saxony and Baden-Wuerttemberg. The average daily milk yield was 2.87 ± 1.20 kg with a fat content of 3.08 ± 0.54% and a protein content of 3.38 ± 0.95%. Somatic cell count (SCC) was transformed into somatic cell score (SCS). Mean SCS was 5.49 ± 1.93. The average lactation length was 234.1 ± 76.4 days. The analysis of variance showed a significant influence of lactation number, stage of lactation, year of lambing and month of lambing on all analysed milk production traits. Milk yield was highest in the fourth lactation number and fat as well as protein content in the 7th to 13th lactation number. Litter size had a significant influence on milk and fat yield and on fat and protein content. The region significantly influenced protein yield and fat and protein content of German Improved Fawn but had no effect on milk and protein yield and SCS. Animals from Baden-Wuerttemberg reached the highest milk protein content but the lowest milk fat content.


Genetika ◽  
2018 ◽  
Vol 50 (1) ◽  
pp. 243-251
Author(s):  
Marina Lazarevic ◽  
Dragan Stanojevic ◽  
Vladan Bogdanovic ◽  
Vlada Pantelic ◽  
Nevena Maksimovic ◽  
...  

The research was performed on Holstein-Friesian and Black and White bull dams reared on five farms of Agricultural Corporation of Belgrade - PKB. The study included 575 lactations of cows selected as bull dams and their progeny calved in the period from 2007 - 2014 and represent progeny of 24 bulls. The following dairy traits were analysed in a standard lactation (305 days): milk yield (kg) - MY, milk fat content (%) - % MF, milk fat yield (kg) - MFY, protein content (%) - % PC and protein yield (kg) - PY. Holstein-Friesian bull dams and their progeny, in standard lactation, produced on average 9239.84 ? 1607.64 kg of milk, with a milk fat content of 3.44 ? 0.20 and protein content of 3.21 ? 0.12. The impact of bull - sire, year of birth, lactation order, farm, year and calving season was present at different levels of statistical significance on yield traits, while the genetic group had no influence on any of the milk traits. Bull - sire, year of birth, lactation order and calving season did not influence the variability of milk fat and protein content. Heritability of observed milk traits was medium to low. The content of milk fat and protein had the lowest values of heritability, 0.014, and 0.024, respectively. The heritability of milk yield, milk fat yield and protein yield was 0.293, 0.319 and 0.273, respectively.


Author(s):  
A. Zheltikov ◽  
N. Kostomakhin ◽  
D. Adushinov ◽  
O. Zaiko ◽  
V. Dementev ◽  
...  

The characteristic of milk productivity of cows of Holstein and Simmental breeds in LLC “Sibirskaya Niva” in the Maslyaninsky district in the Novosibirsk region has been given. It has been revealed that under the natural and climatic environments of the Western Siberia, when creating favorable conditions for feeding and housing for cows of Holstein and Simmental breeds have sufficiently high milk productivity. It has been found that Holstein first-calf heifers surpassed their Simmental herdmates by 959 kg in milk yield for the first 305 days of lactation under the same conditions of housing and feeding, by 3,1 kg in daily milk yield, by 38,2 kg in milk fat yield, and by 28,3 kg in milk protein yield (P < 0,001). There were no statistically significant differences between animals of Holstein and Simmental breeds in terms of fat and protein content in milk, which amounted to 3,94 and 2,96 % in Holstein breed and 3,97 and 2,98 % in Simmental breed, respectively. Both breeds have shown high milk productivity during the first 3 lactations. Thus, the milk yield of Holstein cows for the first 3 lactation was 6475–9290 kg, Simmental cows it was 5516–7680 kg, fat content was 3,93–3,99 and 3,96–3,97 %, respectively. The protein content in the milk of cows of both breeds did not exceed 3,0 %. High variability has been found in milk yield, yield of milk fat and protein, the coefficient of milk content and duration of lactation, the lowest in the content of protein and fat in milk and live weight. The correlation coefficients between the fat and protein content in milk were generally positive, but did not exceed 0,28. Therefore, breeding in the herd of cows in LLC “Sibirskaya Niva” have to be conducted not only by fat content, but also by protein content in milk.


1982 ◽  
Vol 49 (1) ◽  
pp. 25-28 ◽  
Author(s):  
David G. Chamberlain ◽  
Phillip C. Thomas

SUMMARYEight cows in mid lactation and receiving a diet of perennial ryegrass silage and barley (70:30 on a DM basis) were given intravenous supplements of l-methionine (8 g/d). The methionine treatment had no significant effect on milk yield, protein content or lactose content, but increased milk fat content and yield by approximately 10% (P < 0·05).


2020 ◽  
Vol 36 (4) ◽  
pp. 417-426
Author(s):  
Dragan Niksic ◽  
Vlada Pantelic ◽  
Dusica Ostojic-Andric ◽  
Predrag Perisic ◽  
Ljiljana Samolovac ◽  
...  

In order to examine the variability of production performance traits (lactation duration, milk yield for the whole lactation, milk yield in standard lactation, milk fat content, milk fat yield in standard lactation, protein content and yield in standard lactation), 954 cows, domestic and imported Simmental populations, were included in the study, with a total of 3641 completed lactations. Cows were located in the area of Toplica district, reared on individual farming households (tied system) and on the farm with intensive farming (free system). Based on the rearing method and origin, animals were divided into four groups: Group 1 (domestic animals reared by individual agricultural producers on individual farming households); Group 2 (imported animals reared by individual agricultural producers on individual farming households); Group 3 (domestic animals reared on the farm) and Group 4 (imported animals reared on the farm). The fourth group of studied cows showed the highest yield and protein content, while the cows of the third group had the longest lactation and the highest milk fat content. All production performance traits varied highly significantly (p?0.001) under the influence of the combined factor of housing/rearing method and origin.


Author(s):  
Martin Skýpala ◽  
Gustav Chládek

Milk yield varies during lactation, following what is termed a lactation curve. ŽIŽLAVSKÝ and MIKŠÍK (1988) recorded changes in milk yield within a day, too. TEPLÝ et al. (1979) a KOUŘIMSKÁ et al. (2007) published variation within a day ± 1.10 kg in milk yield, ± 0.75 % in milk fat content and ± 0.20 % in milk protein content. Milk yield of cows can be expressed in many different ways, for instance, in kilograms per lactation or in kilograms per day. A practical parameter describing milk production is milk yield (kg) per milking.The object of experiment were 12 cows of Holstein cattle on the first lactation from the 100-day of lactation to 200-day of lactation. The samples of milk were collected from January to May 2007, once a month from the morning and evening milking (milking interval 12 h ± 15 min.). The following parameters were monitored: milk production – milk yield (kg), milk protein production (kg), milk fat production (kg); milk composition – milk protein content (%), milk fat content (%), lactose content (%), milk solids-not-fat content (%), milk total solids content (%); technological properties of milk – ti­tra­tab­le acidity (SH), active acidity (pH), rennet coagulation time (s), quality of curd (class) and somatic cell count as a parameter of udder health.Highly significant differences were found (P < 0.01) between morning milk yield (15.7 kg) and evening milk yield (13.8 kg), between morning milk protein production (0.51 kg) and evening milk protein production (0.45 kg) and between evening milk fat content (4.41 %) and morning milk fat content (3.95 %). A significant difference (P < 0.05) was found between morning milk total solids content (12.62 %) and evening milk total solids content (12.07 %). No significant differences were found between morning (M) and evening (E) values of the remaining parameters: milk fat production (M 0.62 kg; E 0.60 kg), milk protein content (M 3.24 %; E 3.27 %), milk lactose content (M 4.78 %; E 4.86 %), milk solids-not-fat content (M 7.69 %; E 7.71 %), somatic cell count (M 80 000/1 mL; E 101 000/1 mL), titratable aci­di­ty (M 7.75 SH; E 7.64 SH), active acidity (M pH 6.58; E pH 6.61), rennet coagulation time (M 189 s.; E 191 s.), quality of curd (M 1.60 class; E 1.57 class).


Author(s):  
A Gavelis ◽  
V. Þilaitis ◽  
A Juozaitis ◽  
V. Juozaitienë ◽  
G. Urbonavièius ◽  
...  

The aim of this study was to evaluate relationship between milk progesterone concentration (P4) and milk traits at the start of estrus time and 12h after start of the estrus in dairy cows. The 96 milk samples of 48 Lithuanian dairy cows without reproduction disorders and 90–100 days after calving were evaluated. Cows were classified into two groups based on milk yield per day: less than 30 kg (n=20) and e”30 kg (n=28). Data were categorized by milk fat and protein content at the start estrus and 12h after start of estrus to evaluate relationship between P4 and milk traits examined. P4 at estrus time in dairy cows was significantly positively correlated with milk yield (P less than 0.001), whereas it was negatively correlated with milk protein (P less than 0.05-P less than 0.01) and fat at 12h after start of estrus. Dairy cows with F/P from 1.0 to 1.5 had the lowest P4 in milk. Results of the pregnancy in dairy cows were related with lower P4 and milk yield level (P less than 0.001), higher milk fat (P less than 0.05) and milk protein content (P less than 0.001). These cows had 1.90 times lower prevalence of the signs of subclinical ketosis (P less than 0.05) at estrus time when compared with non-pregnant cows. As a result, it was clearly demonstrated that P4 in dairy cows can help to evaluate and improve the reproductive properties of cows.


2021 ◽  
Vol 212 ◽  
pp. 137-142
Author(s):  
E.I. Anisimova ◽  
◽  
P.S. Katmakov ◽  
A.V. Bushov ◽  
◽  
...  

The paper presents results of studies on evaluating Simmental cows for machine milking suitability. It was found that 78.7% of cows had a bowl-shaped udder, round - 14.7% and goat - 6.6% of cows at ‘agricultural production cooperative “COMBINE”. Bowl-shaped cows are more productive. Their milk yield was 4724 kg for 305 days of lactation with a fat content of 3.97% and a protein content of 3.30%. Their udder index was 47.7%. As for cows with a rounded udder shape, milk yield per lactation was 4246 kg with fat and protein content of 4.08 and 3.26% , respectively, and an udder index of 46.1%. Cows with a goat udder shape were characterized by low milk yield (3181 kg) and the worst parametres of milk fat content (3.88%), milk protein (3.20%) and udder index (2.2%). Approximately the same pattern is observed in the herd of the agricultural cooperative “Abodimovskiy” with slightly worse parametres of cow productivity. The intensity of milk production of cows of agricultural production cooperative “Abodimovsiy” with a bowl-shaped udder was 1.42, round - 1.35 and goat - 1.18 kg / min. Cows with a rounded udder shape had higher milk flow rate, which was equal to 1.65 kg / min, with a bowl-shaped one - 1.24 and with a goat udder shape- 1.09 kg / min in agricultural production cooperative “Combine”. No relation was found between udder index and milk composition. There is also no conjugation between the intensity of milk flow and fat and protein content for udder quarters and for the udder as a whole, whereas a positive correlation was found between the intensity of milk flow and daily milk yield.


2016 ◽  
Vol 52 ◽  
pp. 109-114
Author(s):  
K. O. Skoryk

The aim of the work was to study the basic productive traits of Saanen goats of Latvian selection in the order of their selection value: milk yield, fat and protein content in milk. These traits were investigated in the daughters from different goats-fathers. Materials and methods of research. The study was conducted at "Grandmother’s Goats" breeding farm, Galaiky village, Tetiiv district, Kyiv region on Saanen goats of Latvian selection. From mid-spring to mid-autumn the goats grazed and were kept in a corral. In winter, the goats were indoors on deep litter. Milking was twice a day into milk pipeline, it took place in the milking room with 12 seats. The farm is provided enough by coarse, succulent and concentrate feeds. It is considered that the winter diet of dairy goats is hay, twigs, concentrate feed, root vegetables, and mineral supplements. The primary livestock data of milk production in 41 goats for seven lactations were considered. The basic selection traits were studied in the order of their breeding value: milk yield, fat and protein content in milk. The same traits were investigated in terms of fathers of milking goats. The obtained results were processed statistically by N. A. Plohinskiy (1970). Results of research. The research results show that milk yield of Saanen goats of Latvian selection varied depending on lactation. It was found that the milk production during the 1st lactation was 565 kg, fat content in milk – 3.2% and protein content – 3.0%. Milk yield per lactation tended to increase – from the 3rd to 6th lactation inclusively, as compared with the 1st and 7th. The maximum level of milk yield was during the 6th lactation. The differences in milk yield between the 6th and the 1st, 2nd, 5th and 7th lactations are significant with the second degree of reliability. Significant coefficient of variation for milk yield (20-35%) gives reason to consider it possible to conduct the selection by this trait. Several other data are presented by T. Orlovskaya. She considers that goats produce maximum milk yield on average after the 3rd parturition. Milking ability of goats after the 5th-6th lactation begins to fall gradually. She also thinks that biological value of milk is reduced with increase in milk yield, namely reduced fat content and, in some cases, changing the taste of milk. Subsequently, the fat content in milk during the 2nd-4th lactations increased by 0.1%, and during the 5th lactation it reached the maximum level – 3.6%. The protein content in the investigated goat milk was small and ranged from 3 to 3.1%. Differences in fat and protein content during all the lactations were unreliable. Ratio of fat content to protein content in Saanen goats’ milk was studied for seven lactations. It was found that the ratio of fat to milk for all the seven lactations did not reach the optimal levels. This is lack of milk quality of the investigated goats. Milk yield, fat and protein content in milk of daughters of four Saanen billy goats were investigated to determine the influence of fathers on the daughters’ productivity. Analysis of milk productivity data for daughters of goats Amors 046062340091, Wicks 030810040238, Priers 0460623640427 and Frends 038028540074 showed that the highest milk yield was in daughters of Amors 046062340091 – 622 kg. Several less milk yield (590 kg) was in the daughters of Frends 038028540074, and the lowest milk yields were observed in daughters of Wicks 030810040238 and Priers 0460623640427. Thus, daughters’ milk yields depend essentially on fathers. At the same time the fat and protein content in the milk of daughters from different fathers is almost identical. This should be taken into account at matching billy goats for breeding stock of goats. In our research of milk yield of daughters from different billy goats there was a significant difference, but protein and fat content in daughters’ milk almost was no different. Thus, the findings don’t always coincide with the data given in the literature. This concerns with the milk production of Saanen goats and its quality (fat and protein content). Earlier, we have shown that Saanen goats’ milk had a little lower density than in Lamancha, Russian, Nubian, Alpine and Megrelian goats. Protein content was at level of 3.85%, or 1.4% greater than in Russian goats’ milk, but 0.15% less than in Lamancha, 1.23% less than in Nubian, 0.4% – in Alpine, and 1.65% ­– in Megrelian goats’ milk. The fat content in goat milk of the compared breeds was also the lowest (by 1.85% than in Lamancha, by 0.79% than in Russian, by 0.97% than in Nubian, by 0.57% than in Alpine and 1.12% than in Megrelian breeds). Also, content of dry milk residue was low (0.67-1.48% less). Despite these differences in milk yield of goats, research in this area should be expanded in order to determine the prospects for further goat breeding of certain breeds. Conclusions. Milk yield per lactation tended to increase – from the 3rd to 6th lactation inclusively, as compared with the 1st and 7th. The maximum level of milk yield was during the 6th lactation. Significant coefficient of variation for milk yield (20-35%) gives reason to consider it possible to conduct the selection by this trait. The differences in the level of milk production of daughters from different goats were revealed that should be taken into account at matching billy goats for breeding stock. In Ukraine goats’ milk yield of different breeds requires further study.


2020 ◽  
Vol 5 (86) ◽  
pp. 107-114
Author(s):  
А.I. Shendakov ◽  
◽  
◽  

The share of genes of Holstein breed was 76.8% in the herd. It was found that during the research period, the normal distributions of milk productivity traits were observed in the herd. The number of cows in the herd decreased, but their breeding qualities increased. The main reason for which animals were culled from the herd was their gynecological diseases (in some years, up to 175 to 188 heads were culled). The protein content in milk varied from 2.90 to 3.39%. Most cows produced milk in which the protein content was at a level of 3.00 to 3.19%. The herd showed positive correlations between the yields of mothers and daughters for the 305-day-long period: 0.234 for the whole herd, 0.517 for the group of cows with 50% of Holstein breed (HF) genes, 0.098 for the group with 51-75% of HF genes, and 0,189 for the group with minimum 76% of HF genes. For the herd, the heritability coefficient for milk yield for the 305- day-long period (h2) was 0.468. The percent of the protein content in milk produced by cows with 50% of Holstein genes had a high heritability coefficient of 0.668. Only cows of the M. Chieftain line showed positive correlations between the fat content in milk produced by mothers and daughters, and positive correlations between the protein content in milk produced by cows of the W. B. Ideal and M. Chieftain lines was found. With an increase in percent of Holstein breed genes (HF) from 25 to 75% and more, there were no significant differences in milk yield among first-calf cows (they amounted to 5,424 ± 83 – 5,520 ± 116 kg of milk). The percentage of fat and protein in milk for the whole herd was positively interconnected (r = + 0.460). On average, daughters exceeded their mothers by 23.3% in terms of milk yield and by 2.0% in terms of the percentage of fat content in milk (of the trait value). The quantity of milk fat and protein increased by 25.8 and 22.5%, respectively. Based on the research results, it has been concluded that the Holstein breed has a positive effect on the productive traits of dairy cattle, but technology optimization is required to continue selection activities.


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