A Clinical Study on Traumatic Pericarditis and Traumatic Reticuloperitonitis in Twelve Holstein Friesian Cattle

Background: Traumatic injury caused by swallowed sharp foreign object is one of the common conditions in dairy animal resulting into development of traumatic pericarditis (TP) and traumatic reticuloperitonitis (TRP). Under field conditions both conditions mimic the same clinical signs making it difficult to differentiate as well as render to choose ideal therapeutic management. The present study was aimed to evaluate clinical, hematobiochemical and ultrasonographic changes in cattle to clinically differentiate between TP and TRP cases. Methods: From the period of January 2020 to December 2020, total twelve Holstein Friesian cattle were investigated for TP and TRP. In the present study, six animals each suffering from TP and TRP were included along with six normal healthy animals as control. Different clinical signs, haemato-biochemical parameters and ultrasonographical findings were recorded in each group and comparative analysis was done. Result: Brisket edema, bilateral jugular vein engorgement and arched back conditions were most reported clinical signs in both the groups. Significant changes were recorded in the values of red blood cells, lymphocyte, blood urea nitrogen, creatinine and SGOT between both the groups. Significant drop in hemoglobin level was observed in TP affected group. No significant changes were observed in white blood cells, packed cell volume, monocyte counts and eosinophil counts. Significant increase in fibrinogen concentration recorded in both the groups. In ultrasonography, accumulation of anechoic fluid around heart in TP and reticular wall thickening in TRP was most consistent findings.

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Gene expression of the heat stress response in bovine peripheral white blood cells and milk somatic cells in vivo

Scientific Reports ◽

10.1038/s41598-020-75438-2 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

J. B. Garner ◽

A. J. Chamberlain ◽

C. Vander Jagt ◽

T. T. T. Nguyen ◽

B. A. Mason ◽

...

Keyword(s):

Gene Expression ◽

Heat Stress ◽

Blood Cells ◽

White Blood Cells ◽

Somatic Cells ◽

Holstein Friesian ◽

Milk Somatic Cells ◽

Peripheral White Blood Cells ◽

Friesian Cows

Abstract Heat stress in dairy cattle leads to reduction in feed intake and milk production as well as the induction of many physiological stress responses. The genes implicated in the response to heat stress in vivo are not well characterised. With the aim of identifying such genes, an experiment was conducted to perform differential gene expression in peripheral white blood cells and milk somatic cells in vivo in 6 Holstein Friesian cows in thermoneutral conditions and in 6 Holstein Friesian cows exposed to a short-term moderate heat challenge. RNA sequences from peripheral white blood cells and milk somatic cells were used to quantify full transcriptome gene expression. Genes commonly differentially expressed (DE) in both the peripheral white blood cells and in milk somatic cells were associated with the cellular stress response, apoptosis, oxidative stress and glucose metabolism. Genes DE in peripheral white blood cells of cows exposed to the heat challenge compared to the thermoneutral control were related to inflammation, lipid metabolism, carbohydrate metabolism and the cardiovascular system. Genes DE in milk somatic cells compared to the thermoneutral control were involved in the response to stress, thermoregulation and vasodilation. These findings provide new insights into the cellular adaptations induced during the response to short term moderate heat stress in dairy cattle and identify potential candidate genes (BDKRB1 and SNORA19) for future research.

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Experimental infections of baboons (Papio spp.) and vervet monkeys (Cercopithecus aethiops) with Trichinella zimbabwensis and successful treatment with ivermectin

Onderstepoort Journal of Veterinary Research ◽

10.4102/ojvr.v75i2.16 ◽

2008 ◽

Vol 75 (2) ◽

Cited By ~ 10

Author(s):

S. Mukaratirwa ◽

B. M. Dzoma ◽

E. Matenga ◽

S. D. Ruziwa ◽

L. Sacchi ◽

...

Keyword(s):

Packed Cell Volume ◽

Post Infection ◽

Blood Cells ◽

White Blood Cells ◽

Clinical Signs ◽

Blood Parameters ◽

Cercopithecus Aethiops ◽

Vervet Monkeys ◽

Muscular Pain ◽

Normal Ranges

Experimental Trichinella zimbabwensis infections were established in three baboons (Papios p.)and four vervet monkeys (Cercopithecuase thiops) and the clinical-pathological manifestations assessed. The infected animals showed clinical signs ranging from fever, diarrhoea, periorbitaol edema and muscular pain in varying degrees. One baboon became blind due to the infection. Levels of creatinine phosphokinase and lactated ehydrogenase increased to reach a peak on Day 42 post-infection(pi)for both baboons and monkeys. Blood parameters such as packed cell volume, levels of red blood cells and white blood cells did not change significantly from the normal ranges except for the levels of eosinophils which peaked above the normal ranges at Day 28 and 56 pi in baboons and at Day 56 pi in monkeys.

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Biochemical and haematological changes associated with short periods of work in draught oxen

Animal Science ◽

10.1017/s0003356100040368 ◽

1989 ◽

Vol 48 (2) ◽

pp. 375-384 ◽

Cited By ~ 11

Author(s):

R. Anne Pearson ◽

R. F. Archibald

Keyword(s):

Heart Rate ◽

Blood Cells ◽

Inorganic Phosphate ◽

White Blood Cells ◽

Blood Parameters ◽

Blood Levels ◽

Blood Samples ◽

Haematological Changes ◽

Work Done ◽

Friesian Cattle

ABSTRACTBlood samples were taken from three Brahman × Friesian cattle while they walked for 1 h daily on a treadmill pulling 20 or 25 kg weights suspended in a cage. Heart rate and energy expenditure during work were closely correlated. The work had no significant effect on blood levels of red cells, haemoglobin, packed cell volume, total protein, albumin, glycerol, urea, Mg, Ca, Na, K and chloride. White blood cells, glucose, lactate, free fatty acids, P-hydroxybutyrate and inorganic phosphate were affected by work although the changes were shortlived and values had returned to resting levels 75 min after work finished. The changes were similar in each animal and indicated work done by draught cattle is largely at a submaximal level. Apart from lactate no blood parameters were identified that could be usefully used to compare performance.

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Evaluation of clinical signs, parasitemia, hematologic and biochemical changes in cattle experimentally infected with Trypanosoma vivax

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612016013 ◽

2016 ◽

Vol 25 (1) ◽

pp. 69-81 ◽

Cited By ~ 8

Author(s):

Otavio Luiz Fidelis Junior ◽

Paulo Henrique Sampaio ◽

Rosangela Zacarias Machado ◽

Marcos Rogério André ◽

Luiz Carlos Marques ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Acute Phase ◽

Blood Cells ◽

White Blood Cells ◽

Clinical Signs ◽

Chronic Phase ◽

Diagnostic Value ◽

Biochemical Changes ◽

Severe Neutropenia ◽

Trypanosoma Vivax

Abstract Infections by Trypanosoma vivax cause great losses to livestock in Africa and Central and South Americas. Outbreaks due this parasite have been occurred with increasing frequency in Brazil. Knowledge of changes caused byT. vivax during the course of this disease can be of great diagnostic value. Thus, clinical signs, parasitemia, hematologic and biochemical changes of cattle experimentally infected by this hemoparasite were evaluated. Two distinct phases were verified during the infection – an acute phase where circulating parasites were seen and then a chronic phase where fluctuations in parasitemia were detected including aparasitemic periods. A constant reduction in erythrocytes, hemoglobin and packed cell volume (PVC) were observed. White blood cells (WBC) showed pronounced changes such as severe neutropenia and lymphopenia during the acute phase of the illness. Decreases in cholesterol, albumin, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and increases in glucose, globulin, protein, and alkaline phosphatase (ALP) were observed. The “Lins” isolate of T. vivax showed pathogenicity for cattle, and intense parasitemia was detected in the early stages of infection. Circulating parasites were detected for about two months. The most evident laboratory abnormalities were found in WBC parameters, including thrombocytopenia.

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Effectiveness of turmeric-enriched pellets to improve the immunity of Clarias batrachus toward motile Aeromonas septicemia disease

F1000Research ◽

10.12688/f1000research.28260.2 ◽

2021 ◽

Vol 10 ◽

pp. 169

Author(s):

Morina Riauwaty ◽

Yusni I. Siregar ◽

Isma Mulyani

Keyword(s):

Aeromonas Hydrophila ◽

Blood Cells ◽

White Blood Cells ◽

Clinical Signs ◽

Positive Control ◽

Clarias Batrachus ◽

Treatment Groups ◽

Natural Remedy ◽

Loss Of Balance ◽

Treated Fish

Background: Turmeric is known as a natural remedy to improve the immunity of organisms. This study aims to understand the effectiveness of turmeric-enriched pellets to improve the immunity of Clarias batrachus to Aeromonas hydrophila. Methods: The study was conducted from May to August 2020. C. batrachus fingerlings, 7-8 cm total length (TL) and 4-5 g (BW) at baseline, were kept in 30 L aquaria (10 fishes/aquarium; three replicated/treatment). Commercial pellets were mixed with turmeric powder. There were five treatment groups: P0 (control, no turmeric); P1 (0.5 g turmeric per Kg of pellets); P2 (0.7 g/Kg); P3 (0.9 g/Kg); Pp (positive control). Thirty days after being feed with turmeric-enriched pellets, all groups of fish were infected with 0.1 ml (108) of A. hydrophila suspension, intramuscularly. The P0 group did not receive injection, while Pp group were not fed with turmeric-enriched pellets but were infected with the bacteria. Fourteen days after infection, clinical signs and hematology of the fish were studied. Results: Pp fish showed heavy clinical signs of A. hydrophila, such as loss of balance, pigmentation, hemorrhages and ulcers. P0 fish did not show any symptoms, while the treated fish reveled some clinical signs of A. hydrophila to a lesser extent than Pp, indicating that the fish is able to face the A. hydrophila attack. Hematology for Pp fish revealed high white blood cells, indicating that the fish were infected. The blood condition of the P0 fish, as well as those of the turmeric-treated fish were normal. In general, the P3 fish showed the least clinical signs of A. hydrophila and normal blood condition, indicating that P3 treatment is best. Conclusion: The best turmeric dosage to improve the immunity of C. batrachus toward A. hydrophila infection is 0.9 g/Kg pellets.

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Impact of early castration and health status on the performance of Holstein-Friesian and beef crossbred calves

Journal of Veterinary Research ◽

10.1515/jvetres-2016-0030 ◽

2016 ◽

Vol 60 (2) ◽

pp. 207-212

Author(s):

Zofia Wielgosz-Groth ◽

Monika Sobczuk-Szul ◽

Zenon Nogalski ◽

Cezary Purwin ◽

Paulina Pogorzelska-Przybyłek ◽

...

Keyword(s):

Health Status ◽

Blood Cells ◽

White Blood Cells ◽

Rubber Band ◽

Blood Parameters ◽

Grass Silage ◽

Holstein Friesian ◽

Body Weights ◽

Similar Body ◽

Milk Feeding

AbstractIntroduction:This article presents the analysis of the correlation between the category and health status of calves and the results of their rearing and levels of selected blood parameters.Material and Methods:The study included 105 Polish Holstein-Friesian and beef (Limousine, Charolaise and Hereford) crossbred calves. Young bulls were purchased at the age of two to four weeks. The animals underwent quarantine, were dehorned, and 46 young bulls were castrated. The germ horns were removed by burning out. Castration was carried out with a bloodless method using a rubber band. The calves were kept in groups and fed a milk replacer administeredviateats from automated milk-feeding stations. After the period of milk feeding, the calves were fed grass silagead libitumand a concentrate at 2.5 kg/animal/day. The calves were weighed every two weeks. Blood for analyses was sampled at 43 d of age.Results:After the rearing period finished at the age of six months, young bulls and steers had similar body weights (176.17 and 176.55 kg) and approximate average daily weight gains from birth (0.756 and 0.767 g/day). The healthy calves at six months of age weighed 180.47 kg, whereas the animals which at least once suffered from some diseases during rearing were lighter by approx. 30 kg (P ≤ 0.01). A statistically significant (P ≤ 0.01) difference was found for the count of red blood cells and white blood cells. In comparison with healthy individuals, the diseased animals had less RBC (8.33 and 9.42 1012/L respectively) and more WBC (27.03 and 12.26 109/L respectively).Conclusion:Castration of young bulls did not have any impact on the results of rearing and health status of the calves. The magnitude of the analysed parameters depended on the health status of the calves. Thus RBC and WBC parameters may be used to predict the health status of calves during rearing.

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Effect of exercise on physiological, blood and endocrine parameters in search and rescue-trained dogs

Veterinární Medicína ◽

10.17221/1860-vetmed ◽

2008 ◽

Vol 53 (No. 6) ◽

pp. 333-346 ◽

Cited By ~ 34

Author(s):

S. Rovira ◽

A. Munoz ◽

M. Benito

Keyword(s):

Blood Cells ◽

Reference Range ◽

Venous Blood ◽

Recovery Period ◽

White Blood Cells ◽

Clinical Signs ◽

Search And Rescue ◽

Exercise Intolerance ◽

Exercise Heart Rate ◽

Total Plasma Protein

Exercise induces a variety of physiological and laboratorial changes of different magnitude and direction, depending on the characteristics of the performed exercise (duration and intensity) and on the fitness and training level of the dog. The present research aims to describe the normal response to a session of search and rescue exercise in trained dogs in order to distinguish these changes from those derived from exhaustion or diseases. Nine healthy and trained dogs of both sexes (five females and four males), aged between 24 months and seven years (mean: 3.5 years) were studied. Exercise consisted in a normal session of searching and rescue training of 20 min of duration, carried out in an open terrain. During the exercise, heart rate (HR) was monitored continuously with a HR-meter. Furthermore, respiratory rate (RR) and rectal temperature (RT) were measured and venous blood samples were extracted at rest (R), immediately after exercise (E) and at 5, 15 and 30 min of a passive recuperation (5REC, 15REC and 30REC). The following laboratorial parameters were studied: red blood cells (RBC), hemoglobin concentration (HB), packed cell volume (PCV), RBC volumetric indices, white blood cells (WBC), creatinine (CREAT), total plasma protein (TPP), lactate (LA), glucose (GLU), triacylglycerols (TAG), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), Na, K, Cl, cortisol (CORT) and insulin (INS). Clinical signs indicative of exhaustion or exercise intolerance were not observed in the dogs during the study. HR increased with E and remained over the reference range until 30REC. RR and RT also rose with E, with the highest RR at 5REC. RBC, HB and PCV were not affected by E, whereas WBC increased at E. TPP, GLU, AST and K were not affect by E neither by REC. E induced elevations in CK, LDH, LA and INS, reaching R values at 30REC, 30REC, 15REC and 5REC, respectively. Plasma Na decreased with E and recovered at 30REC. Plasma Cl decreased with E, without additional significant changes. Circulating CORT concentrations were reduced with E, with the highest reduction at 10REC. Modifications of RR, RT, WBC, CREAT and TAG persisted throughout the recovery period. In conclusion, significant modifications in physiological and laboratorial parameters were induced by the searching and rescue exercise, with values outside the reference range for healthy dogs. These data provide a data base for evaluating ill or injured dogs during this type of exercise. In addition, there was not evidence of dehydration, electrolyte imbalances, and stress or muscle disorders in the studied dogs.

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The Histone Deacetylase 1 and 2 (HDAC1/2) Inhibitor ACY-957 Increases Epsilon (HbE) and Gamma (HbG) Globin mRNA in the Peripheral Blood of Non-Anemic Rats and Monkeys

Blood ◽

10.1182/blood.v126.23.3378.3378 ◽

2015 ◽

Vol 126 (23) ◽

pp. 3378-3378 ◽

Cited By ~ 1

Author(s):

Jeffrey R Shearstone ◽

Apurva Chonkar ◽

Kailash Bhol ◽

Simon S Jones ◽

Matt Jarpe

Keyword(s):

Peripheral Blood ◽

Blood Cells ◽

Plasma Levels ◽

White Blood Cells ◽

Clinical Signs ◽

High Dose ◽

Employment Equity ◽

Once Daily ◽

Equity Ownership ◽

Baseline Levels

Abstract Elevated levels of HbG mRNA, leading to the formation of fetal hemoglobin, is known to ameliorate disease severity in sickle cell and β-thalassemia patients. We have previously shown that the small molecule ACY-957 is a selective inhibitor of HDAC1/2 which induces HbE and HbG in cultured human primary CD34+ cells (Shearstone et al, ASH Annual Meetings 2012-14). In this work, we describe the pharmacokinetics and HbE/HbG induction following once daily oral dosing of ACY-957 in rat and monkey. To determine the duration of ACY-957 exposure required to induce HbE and HbG in vivo, we first tested ACY-957 in drug washout experiments performed in cultured primary erythroid progenitors. The aminobenzamide class of HDAC inhibitors, such as ACY-957, are known to have slow on rates for HDAC1/2 (Kral et al, Biochemistry 2014; Lauffer et al, J Biol Chem 2013). We found that a 6 h pulse of ACY-957 (1 μM) resulted in undetectable increases in histone H3 lysine 9 acetylation (H3K9ac) and that 4 or 8 h pulses of ACY-957 (1 μM) resulted in undetectable HbE and HbG induction. However, continued exposure resulted in a 2.5-fold and 7-fold increase in H3K9ac after 24 and 48 h of incubation, respectively, leading to a time-dependent increase in HbE and HbG. Based on this data, we hypothesized that in vivo studies would require ACY-957 levels of 1 μM for 24 h in order to observe elevated HbE and HbG. Non-fasted Sprague Dawley rats or cynomolgus monkeys received a single oral dose of 20 mg/kg or 12.5 mg/kg ACY-957, respectively, and pharmacokinetic analysis yielded comparable results with T1/2 = 11.8 and 10.9 h, Cmax = 7.8 and 2.4 μM, and Tmax = 5.3 and 4.0 h, in rat and monkey, respectively. At 24 h post dose, ACY-957 plasma levels in rat and monkey were 1.6 and 0.6 μM, respectively. These findings suggested that the targeted drug exposure could be met with a single daily oral dose of ACY-957. Since ACY-957 induced HbE in cultured human primary erythroid progenitors, we attempted to measure HbE induction in rat as a surrogate marker for HbG in primate. Rats were dosed with 0, 10 or 30 mg/kg (n=4 per group) by oral gavage, once daily for 6 days, followed by a 13 day washout period. Peripheral blood was sampled every 3 days for isolation of total RNA. Complete blood counts were performed on day 0, 6 and 18. The low and high dose groups showed ACY-957 plasma levels of 1.3 or 5.2 μM, respectively, at 24 h post final dose. No abnormal clinical signs were found during the in-life phase, although a minor, reversible delay in rat weight gain was observed in the high dose group. White blood cells were suppressed by 33% and 68% at day 6 in low and high dose groups, respectively, but recovered to baseline levels by day 18. ACY-957 administration led to a dose-dependent increase in HbE relative to HbB that was detectable at day 3, peaked at day 6, and returned to baseline levels by day 9. Maximum induction of HbE was 2-fold and 5.6-fold for the low and high dosing groups, respectively, relative to animals receiving vehicle only. Next, monkeys were dosed at 0, 25 or 75 mg/kg (n=3 per group) by oral gavage, once daily for 5 days, followed by a 14 day washout period. Peripheral blood was sampled every 2 to 3 days for isolation of total RNA and analysis of complete blood counts. The low and high dose groups showed ACY-957 plasma levels of 1.9 or 8.0 μM, respectively, at 24 h post final dose. No abnormal clinical signs were found during the in-life phase. White blood cells were suppressed by 25% and 61% at day 5, but recovered to baseline levels by day 9. ACY-957 administration led to a dose-dependent increase in HbE and HbG relative to HbB that was detectable at day 5, peaked at day 7, and returned to baseline levels by day 12. Maximum induction of HbG was 2.2-fold and 7.2-fold for the low and high dosing groups, respectively, relative to animals receiving vehicle only. These results demonstrate that ACY-957 induces HbE in rat and HbG in monkey to a similar extent. ACY-957 appeared well tolerated in both animals, although a reversible suppression of white blood cells was observed. Together, these findings suggest that optimization of dose and schedule could be performed in rats by monitoring HbE as a surrogate for HbG in primates. The optimized regime could then be validated in cynomolgus monkey. Accordingly, we have initiated experiments that explore the effects of several different ACY-957 dose schedules on HbE induction and white blood cell suppression in rats during a 4 week dosing and 2 week recovery period, which will also be presented. Disclosures Shearstone: Acetylon Pharmaceuticals, Inc.: Employment, Equity Ownership. Chonkar:Acetylon Pharmaceuticals, Inc.: Employment, Equity Ownership. Bhol:Acetylon Pharmaceuticals, Inc.: Employment, Equity Ownership. Jones:Acetylon Pharmaceuticals, Inc.: Employment, Equity Ownership. Jarpe:Acetylon Pharmaceuticals, Inc.: Employment, Equity Ownership.

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Impact of Central Nervous System Involvement in Adult Patients with Acute Lymphoblastic Leukemia Treated in a Pediatrics-Inspired Protocol - a Graall Study

Blood ◽

10.1182/blood-2021-150705 ◽

2021 ◽

Vol 138 (Supplement 1) ◽

pp. 215-215

Author(s):

Corentin Orvain ◽

Sylvain Chantepie ◽

Xavier Thomas ◽

Martine Escoffre-Barbe ◽

Francoise Huguet ◽

...

Keyword(s):

Blood Cells ◽

Research Funding ◽

Lymphoblastic Leukemia ◽

White Blood Cells ◽

Clinical Signs ◽

Adult Patients ◽

High Dose ◽

Cns Involvement ◽

The Impact ◽

To Receive

Abstract Background: The prognosis of central nervous system (CNS) involvement in adult patients with acute lymphoblastic leukemia (ALL) has been historically associated with a dismal outcome. Whereas the prognosis of adult patients with ALL has greatly improved since the advent of pediatrics-inspired regimens, the prognostic impact of CNS involvement has not been formerly reevaluated. We report herein the impact of CNS involvement in patients included in the pediatric-inspired prospective GRAALL-2005 study. Methods: All patients received a 5-drug induction therapy with native E. Coli-ASP intravenous injections. Patients in complete remission (CR) received two consolidation courses with alternating cycles including high dose cytarabine (2g/m2/12h on days 1 and 2), high dose methotrexate (3 g/m2 on day 1), and cyclophosphamide. All patients in persistent CR and with no indication for allogeneic stem cell transplantation (SCT) received late intensification, followed by one last consolidation course. Patients with initial CNS involvement, clinically and/or cytologically (cerebrospinal fluid), were recommended to receive an increased number of triple intrathecal therapy, CNS irradiation, and were eligible for allogeneic SCT in first CR. They received less Asp injections during induction therapy to avoid CNS adverse events. CNS irradiation included two lateral fields encompassing the skull, facial, the base of the skull, and the first two cervical vertebrae at a dose of 24 grays for those not receiving allogeneic SCT and 15 grays for those receiving allogeneic SCT. Results: Between 2006 and 2014, 784 adult patients with newly diagnosed Philadelphia-negative ALL were included with 55 (7%) having initial CNS involvement. These patients were more likely to be of T-phenotype (51 versus 32%, p=.004) and had more white blood cells at diagnosis (median 23 G/l versus 11 G/l, p=.02). Most patients (36 pts, 66%) were classified as CNS-3 (> 5 white blood cells/µl and a positive cytospin and/or clinical signs) whereas 5 patients (9%) were CNS-2 (< 5 white blood cells/µl and a positive cytospin), and 14 (25%) have data pending. Among patients with details regarding CNS involvement, 25/41 (61%) had clinical signs including trigeminal anesthesia (9 pts, 36%), facial paralysis (4 pts, 16%), extremities paresthesia (4 pts, 16%), visual signs (2 pts, 8%), meningeal syndrome (2 pts, 8%), and motor deficit (2 pts, 8%), and 4/18 (22%) had radiological signs. Induction death, CR1 rate, and negative minimal residual disease after induction were similar whether patients had CNS involvement or not (6 vs 6%, 89 vs 89%, 73 vs 62%, 26 vs 22%, respectively). Patients with CNS involvement had a worse outcome than those without with a median event-free survival (EFS) of 391 days (versus not reached for patients without CNS involvement, HR: 1.7, 95% CI: 1.2 - 2.5, p=.002) and a median overall survival (OS) of 608 days (versus not reached for patients without CNS involvement, HR: 1.8, 95% CI: 1.3 - 2.6, p=.001) (figure). Similar results were observed when patients who received allogeneic SCT in CR1 were censored at the time of graft. As recommended, patients with CNS involvement were more likely to receive allogeneic SCT than those without (53 versus 34%, p=.01), with a median time of 169 days. A 150-day landmark analysis, excluding 12 patients with an EFS event before 150 days, was performed to study the impact of allogeneic SCT on the outcome of patients with CNS involvement. Allogeneic SCT had no impact on either EFS (HR: .5, 95% CI: .2 - 1.2, p=.15) or OS (HR: .8, 95% CI: .3 - 1.8, p=.53). Conclusion: Despite improved outcome in young adult ALL patients with pediatrics-inspired protocols, CNS involvement remains a poor-risk feature. The historical use of allogeneic SCT does not improve outcome. Specific regimens should be developed for adult ALL patients with CNS involvement. Figure 1 Figure 1. Disclosures Huguet: Amgen: Other: Advisor; BMS: Other: Advisor; Celgene: Other: Advisor; Jazz Pharmaceuticals: Other: Advisor; Novartis: Other: Advisor; Pfizer: Other: Advisor. Barbieux: ASTRA-ZENECCA: Consultancy. Vey: Amgen: Honoraria; BMS: Honoraria; BIOKINESIS: Consultancy, Research Funding; NOVARTIS: Consultancy, Honoraria, Research Funding; SERVIER: Consultancy; JAZZ PHARMACEUTICALS: Honoraria; JANSSEN: Consultancy. Dombret: Amgen: Honoraria, Research Funding; Incyte: Honoraria, Research Funding; Jazz Pharmaceuticals: Honoraria, Research Funding; Novartis: Research Funding; Pfizer: Honoraria, Research Funding; Servier: Research Funding; Abbvie: Honoraria; BMS-Celgene: Honoraria; Daiichi Sankyo: Honoraria. Boissel: Bristol-Myers Squibb: Honoraria, Research Funding; Servier: Consultancy, Honoraria; Incyte: Honoraria; Amgen: Consultancy, Honoraria, Research Funding; Novartis: Consultancy, Honoraria, Research Funding; JAZZ Pharma: Honoraria, Research Funding; CELGENE: Honoraria; SANOFI: Honoraria; PFIZER: Consultancy, Honoraria. Mathilde: ABBVIE: Consultancy; SERVIER: Consultancy.

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