Selection of promising genotypes of lentil (Lens culinaris Medik.) by deciphering genetic diversity and trait association

Author(s):  
A.K. Parihar ◽  
Sarvjeet Singh ◽  
R.K. Gill ◽  
R. Kumar ◽  
A.K. Parihar

A panel of fifty diverse genotypes of lentil were evaluated to assess the genetic diversity for 11 morpho-physiological attributes. The results revealed wide range of variability among the genotypes for the examined characteristics. The mean performance demonstrated that the best genotype for more than one trait viz., plot yield, yield/plant, Leaf Area Index (LAI), Crop Growth Rate (CGR), biomass and plant height was LL1384. Traits association approximation witnessed a positive and significant relationship of seed yield with most of the studied traits. Cluster analysis distributed genotypes into seven distinct clusters. Cluster III happened to be the largest comprising of 20 genotypes, followed by Cluster VII comprising of 9 genotypes. The maximum inter-cluster distance was perceived between clusters II and IV which suggests the possibility of high heterotic effect if cross will be made between the individuals of these clusters. The principal component analysis developed 11 Eigen vectors and, of these three principal components had explained about 82.50% of variations. The characters that had the highest weight in component first were plot yield, yield/plant, pods/plant, CGR, biomass/plant, LAI and plant height, which explained 57.03% of the total variance. The biplot results were in accordance with correlation estimation, indicating that indirect selection based on component traits would help in improving the seed yield in lentil. Hybridization among genetically diverse genotypes of different clusters would be helpful in broadening the genetic base as well as in the creation of desirable recombinants for developing new improved lentil varieties.

Author(s):  
MJ Hasan ◽  
MU Kulsum ◽  
MZ Ullah ◽  
M Manzur Hossain ◽  
M Eleyash Mahmud

A study on genetic diversity was conducted with 54 Chili (Capsicum annuum L.) genotypes through Mohalanobis’s D2 and principal component analysis for twelve quantitative characters viz. plant height, number of secondary branch/plant, canopy breadth , days to first flowering, days to 50% flowering, fruits/plant, 5 fruits weight, fruit length, fruit diameter, seeds/fruit, 1000 seed weight and yield/plant were taken into consideration. Cluster analysis was used for grouping of 54 chili genotypes and the genotypes were fallen into seven clusters. Cluster II had maximum (13) and cluster III had the minimum number (1) of genotypes. The highest inter-cluster distance was observed between cluster I and III and the lowest between cluster II and VII. The characters yield/plant, canopy breadth, secondary branches/plant, plant height and seeds/fruit contributed most for divergence in the studied genotypes. Considering group distance, mean performance and variability the inter genotypic crosses between cluster I and cluster III, cluster III and cluster VI, cluster II and cluster III and cluster III and cluster VII may be suggested to use for future hybridization program. DOI: http://dx.doi.org/10.3329/ijarit.v4i1.21088 Int. J. Agril. Res. Innov. & Tech. 4 (1): 32-35, June, 2014


1970 ◽  
Vol 23 (1) ◽  
pp. 9-12 ◽  
Author(s):  
AA Jewel ◽  
AK Chowdhury ◽  
AKMM Alam ◽  
MA Latif ◽  
MM Hassan

Genetic divergence among 22 genotypes of lentil was estimated using D2 and principal component analysis. The genotypes under study fell into four clusters. The intra-cluster value was the maximum in cluster I and the minimum in cluster II. The inter-cluster distance was larger than the intra-cluster distance in all the cases suggesting the wider genetic diversity among the genotypes of different groups. The inter-cluster value indicated maximum distance between cluster I and II than cluster III and IV. Thus the genetically diverged genotypes of the clusters could be used as parent in hybridization program to get desirable genotypes. Cluster I had the highest mean for days to maturity, plant height, pods/plant and per plot yield while cluster II and III had the highest days to first flowering and 100 seed weight, respectively. Genotypes with these characters in respective groups would, therefore, offer a good scope of improvement of lentil through selection.   Key words: Lentil; cluster analysis; divergence; hybridization DOI: http://dx.doi.org/10.3329/bjpbg.v23i1.9312 BJPBG 2010; 23(1): 9-12


Author(s):  
S.K. Jain ◽  
L.D. Sharma ◽  
K.C. Gupta ◽  
Vipin Kumar ◽  
R.S. Sharma

Background: The seed yield of chickpea can be improved by selection of superior genotypes on the basis of different yield and yield component traits. These genotypes exclusively utilize in breeding programs. Yield is a complex trait which is affected by several factors, hence, a well-known technique known as principal component analysis was used to identify and minimize the number of traits for effective selection. To obtain efficient recombinants, the identified component traits need to be combined from diverse parents through recombination breeding followed by selection of transgressive segregants. Hence, the present study is envisaged to measure the genetic diversity among genotypes of chickpea.Methods: The experimental material comprised of 40 chickpea genotypes evaluated in randomized block design with three replications. The experimental unit was four rows per plot with 4 m length and spacing between row to row and plant to plant maintained as 30 x 10 cm. NPK (20:40:00) fertilizers was applied as basal doses. The data were recorded for each genotype on nine quantitative traits as per standard methods. Descriptive statistics and PCA analysis was performed by using the statistical package SPSS 16.0 version and cluster analysis was done using the Wards method of hierarchical clustering technique.Result: Out of nine PCs only three PCs exhibited more than 1.0 Eigen value and showed about 73.4% variability. PC1 contributed 28.6% of the total variation and correlated with days to flowering, days to maturity, plant height, first pod height, seeds per pod and number of pods per plant while PC2 explained 21.00% of the total variation and dominated by plant height, first pod height and seed yield. PC3 explained an additional 13.00% of the total variation and dominated by primary branches per plant. Genotype commonly found in more PC, were BG 4016, IPCB 2015-165, IPC 2011-247, GNG2459 and RKG 19-4. Hierarchical clustering technique grouped 40 genotypes into two main clusters (A and B) and nine sub clusters. The present investigation depicted that the chickpea germplasm displayed considerable genetic diversity for most of the traits under consideration. 


2016 ◽  
Vol 50 (1) ◽  
Author(s):  
P. Y. Kamannavar ◽  
S. B. Revanappa ◽  
A. G. Vijaykumar ◽  
K. Basamma ◽  
Ganajaxi

Genetic diversity was assessed in thirty urdbean cultivars including advance breeding lines by Mahalanobis D<sup>2</sup> statistics using seed yield and its component morphological traits. Analysis of genetic diversity revealed considerable amount of diversity among the genotypes and led to their grouping into eight clusters. The cluster-I comprising 14 genotypes was the largest followed by cluster-II and III with 5 and 4 genotypes respectively. The remaining clusters were monogenotypic. Pods per plant (32.5%), seed yield per plant (30%) and plant height (21.5%) were mainly responsible for genetic diversity. Based on inter-cluster distance and mean performance of clusters for different traits, the crosses among genotypes like, DBG-5, DU-1, Manikya, KU-7638, and DBG-3 are expected to produce desirable segregants for yield and other yield related traits.


2014 ◽  
Vol 39 (2) ◽  
pp. 189-196 ◽  
Author(s):  
MF Amin ◽  
M Hasan ◽  
NCD Barma ◽  
MG Rasul ◽  
MM Rahman

Genetic divergences of 50 wheat lines were studied through Mohalanobis’s D2 and principal component analysis for fourteen characters. Genotypes were grouped into four different clusters. Cluster II comprised maximum number of genotypes (twenty one) followed by cluster IV. The inter-cluster distance was maximum between clusters I and III (12.29) indicating wide genetic diversity between these two clusters followed by the distance between cluster I and cluster II (8.28), and cluster III and cluster IV (7.97). The minimum inter-cluster distance was observed between cluster II and cluster IV (4.193) followed by cluster I and cluster IV (4.339) and cluster II and cluster III(4.390) indicating that the genotypes of these clusters were genetically close. The intra cluster distance of all the four clusters was more or less low which indicated that the genotypes within the same cluster were closely related. The highest inter genotypic distance (0.9166) was observed between the genotypes G 40 and G 41and lowest (0.0993) between the genotypes G 22 and G 43. Among the characters, heading days, maturity days, plant height (cm), canopy temperature at vegetative stage, canopy temperature at grain filling stage, grain filling rate (g d-1m-2), 1000- grain weight (g), and grains spike-1 contributed most for divergence in the studied genotypes. Cluster I had the highest mean for grain yield (4711.2 kg/ha), grain filling rate (17.5 g d-1m-2), chlorophyll content at anthesis, and plant height (93 cm). Crosses between I & III, I & II, and III & IV have greater chances to generate more heterotic F1s. Considering magnitude of genetic distance, contribution of different traits toward the total divergence, magnitude of cluster means for different traits and performance the genotypes G10, G 11, G12, G35, G40, G48 of cluster I, G7 of cluster II, G41, G5,and G3 of cluster III and G46, G21 of cluster IV may be considered as good parents for future hybridization program to produce high yielding genotypes. DOI: http://dx.doi.org/10.3329/bjar.v39i2.20414 Bangladesh J. Agril. Res. 39(2): 189-196, June 2014


Author(s):  
M. Karthikeyan ◽  
Sharad Pandey ◽  
Gideon Synrem ◽  
K. R. Saravanan

An experiment using twenty genotypes of chickpea (Cicer arietinum L.) was conducted during Rabi season of 2019-2020, to find the genetic diversity using D2 statistics. The experiment was laid out in randomized block design with three replications at the experimental field of Himgiri Zee University, Dehradun. The observations was recorded on  nine quantitative characters where five randomly selected plants were taken the average was computed while the traits days to 50 % flowering and days to 100 % maturity was taken from plot basis. Results revealed that the genotypes were grouped into 4 clusters where Cluster-I had fifteen genotypes and cluster II had three genotypes while one genotype each was present in cluster III and IV. The seed yield per plant contributed maximum towards genetic diversity (32.00 %) followed by plant height at maturity (14.00 %) and number of secondary branches (9.00 %). The maximum intra cluster distance was found in cluster II (164.691) indicating that the 15 genotypes including in the cluster II were most divergent. However, maximum inter cluster distance was noticed between cluster I and cluster II (313.247) which could be used in hybridizing program.


Author(s):  
Moushree Sarkar ◽  
Sabyasachi Kundagrami

An experiment to evaluate eleven agro morphological traits in twenty three genotypes of mungbean [Vigna radiata (L.) Wilczek] in the field experiment was conducted in two consecutive years 2013 and 2014. Data were recorded on quantitative as well as visually assayed agro morphological traits including days to 50 per cent flowering, days to 1st picking of pods, days to final picking, plant height (cm), number of secondary branches per plant, number of pods per plant, pod length (cm), number of seeds per pod, 100 seed weight (g), seed yield/plant (g) and harvest index (%). All the traits were analysed using multivariate statistical analysis. Most of the important evaluated traits showed a wide range of variation. Cluster analysis using UPGMA method grouped the genotypes into five clusters. The component of mungbean genotypes among different clusters was varied from two to nine genotypes. The maximum number of genotypes i.e., 9 is found in cluster III followed by cluster IV comprising of 6 genotypes. Cluster V showed the maximum mean value for plant height, branch/plant, pods/plant, seed/pod, seed yield/plant and lowest values for days to 50% maturity, 1st picking and days to maturity. Principal component analysis revealed that the first five main PCAs amounted 71.11% of the total variation among genotypes. PC1 accounts for maximum variability in the data with respect to succeeding components.


2021 ◽  
Vol 18 (2) ◽  
pp. 27-37
Author(s):  
MT Islam ◽  
BN Shompa ◽  
J Rahman

Genetic diversity of twenty-one genotypes of tossa jute was studied through Mahalanobis D2 statistic and principal component analysis for 15 quantitative traits related to yield at the research farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. The genotypes were grouped into four clusters. Cluster III contained the highest number of genotypes (nine), while cluster II had the lowest number of genotypes (three). The inter-cluster distances were higher than intra-cluster distances indicating wider genetic diversity among the genotypes of different clusters. The intra-cluster distances were lower in all the cases reflecting homogeneity of the genotypes within the cluster. The highest intra-cluster distance was noticed for the cluster I and the lowest for cluster IV. The genotypes from cluster I could be selected as parents for hybridization for producing transgressive segregants. The highest inter-cluster distance was found between clusters I and IV followed by clusters I and III and the lowest was between clusters III and IV. Cluster IV recorded the highest mean value for plant height, base diameter, root length, leaf area, fresh weight with and without leaves, dry fiber weight and dry stick weight. In the total divergence, the highest contribution was recorded in plant height (65.1%) followed by base diameter (16.6%). The first two principal components, whose eigen values were greater than one, accounted for 81.7% of the total variations among the genotypes for fifteen fiber related traits. Considering the magnitude of cluster distance, cluster means for different characters and contribution of characters towards divergence, the genotypes G17, G18 from cluster IV; G1, G2, G5, G10, G16 from cluster I and G4 from cluster III could be selected as promising parents for hybridization program. SAARC J. Agri., 18(2): 27-37 (2020)


Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 680
Author(s):  
Thuy T. P. Mai ◽  
Craig M. Hardner ◽  
Mobashwer M. Alam ◽  
Robert J. Henry ◽  
Bruce L. Topp

Macadamia is a recently domesticated Australian native nut crop, and a large proportion of its wild germplasm is unexploited. Aiming to explore the existing diversity, 247 wild accessions from four species and inter-specific hybrids were phenotyped. A wide range of variation was found in growth and nut traits. Broad-sense heritability of traits were moderate (0.43–0.64), which suggested that both genetic and environmental factors are equally important for the variability of the traits. Correlations among the growth traits were significantly positive (0.49–0.76). There were significant positive correlations among the nut traits except for kernel recovery. The association between kernel recovery and shell thickness was highly significant and negative. Principal component analysis of the traits separated representative species groups. Accessions from Macadamia integrifolia Maiden and Betche, M. tetraphylla L.A.S. Johnson, and admixtures were clustered into one group and those of M. ternifolia F. Muell were separated into another group. In both M. integrifolia and M. tetraphylla groups, variation within site was greater than across sites, which suggested that the conservation strategies should concentrate on increased sampling within sites to capture wide genetic diversity. This study provides a background on the utilisation of wild germplasm as a genetic resource to be used in breeding programs and the direction for gene pool conservation.


2013 ◽  
Vol 37 (4) ◽  
pp. 617-624 ◽  
Author(s):  
MJ Hasan ◽  
Umma Kulsum ◽  
MMH Rahman ◽  
MMH Chowdhury ◽  
AZMKA Chowdhury

Genetic divergence of 40 parental lines comprising 30 restorer and 10 maintainer lines were studied through Mohalanobis's D2 and principal component analysis for eleven characters. Genotypes were grouped into five different clusters. Cluster V comprised maximum number of genotypes (thirteen) followed by cluster I and II. The inter-cluster distance was maximum between clusters I and V (13.495) indicating wide genetic diversity between these two clusters followed by the distance between cluster I and 11 (9.489), cluster IV, and cluster V (8.969) and cluster I and cluster III (8.039). The minimum inter-cluster distance was observed between cluster II and cluster III (3.034) followed by cluster 111 and cluster IV (3.834) and cluster II and cluster V (4.945) indicating that the genotypes of these clusters were genetically close. The intra cluster distance in the entire five clusters was more or less low which indicated that the genotypes within the same cluster were closely related. Among the characters panicle weight contributed most for divergence in the studied parental lines. Difference in cluster means existed for almost all the characters studied. Highest mean value for number of effective tillers (7.8), days to 50% flowering (95.5), panicles/m2 (192.6), panicle weight (2.9), spikelet fertility (84.8), number of grains/panicle (177.8), days to maturity (123.6), and grain yield/plot (1065.5) were observed in cluster I indicated the parental lines fallen in this cluster having the genetic potentiality to contribute better for yield maximization of hybrid rice. DOI: http://dx.doi.org/10.3329/bjar.v37i4.14386 Bangladesh J. Agril. Res. 37(4): 617-624, December 2012


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