Antibacterial activity of the seeds, roots and shoots of Lotus populations

2018 ◽  
Author(s):  
Gurkan Demirkol
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Plant Pathogens ◽  
Plant Diseases ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Clavibacter Michiganensis ◽  
High Antibacterial Activity ◽  
Agar Dilution

In this study the antibacterial activity of the ethanol, ethyl acetate and chloroform extracts from the seeds, roots and shoots of Lotus aegaeus, Lotus angustissimus, Lotus corniculatus, Lotus gebelia, Lotus palustris populations grown naturally in Turkey were investigated by using the disc diffusion and agar dilution method, against main plant pathogenic bacteria (Clavibacter michiganensis, Agrobacterium tumefaciens, Erwinia caratovora, Pseudomonas phaseolicola). According to results, the shoot ethyl acetate extract of L. aegaeus and shoot extracts of L. corniculatus against C. michiganensis and shoot extracts of all solvents of L. angustissimus against P. phaseolicola showed high antibacterial activity. This is the first report of antibacterial activity of the Lotus species against plant pathogens. In the study these stated effective extracts showed higher antibacterial effects by comparison with used chemical preservatives against sensitive bacteria. This study offers that active compounds present in Lotus species could play a big role in naturally plant preservation against plant diseases.

scholarly journals Antifungal and Antibacterial Metabolites from a French Poplar Type Propolis

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Séverine Boisard ◽  
Anne-Marie Le Ray ◽  
Anne Landreau ◽  
Marie Kempf ◽  
Viviane Cassisa ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Gram Positive ◽  
Weak Activity ◽  
Agar Dilution

During this study, thein vitroantifungal and antibacterial activities of different extracts (aqueous and organic) obtained from a French propolis batch were evaluated. Antifungal activity was evaluated by broth microdilution on three pathogenic strains:Candida albicans, C. glabrata, andAspergillus fumigatus. Antibacterial activity was assayed using agar dilution method on 36 Gram-negative and Gram-positive strains includingStaphylococcus aureus. Organic extracts showed a significant antifungal activity againstC. albicansandC. glabrata(MIC80between 16 and 31 µg/mL) but only a weak activity towardsA. fumigatus(MIC80= 250 µg/mL). DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially againstS. aureus(SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC10030–97 µg/mL). A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.

scholarly journals Antibacterial Diterpenes from the Roots of Ceriops tagal

2008 ◽  
Vol 3 (1) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Musa Chacha ◽  
Renameditswe Mapitse ◽  
Anthony J. Afolayan ◽  
Runner R. T. Majinda
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Spectroscopic Data ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Bacterial Strains ◽  
Agar Dilution ◽  
Ceriops Tagal

Investigation of the roots of Ceriops tagal led to the isolation of a new isopimarane, together with the known diterpenes isopimar-8(14)-en-15,16-diol and erythroxyl-4(17),15(16)-dien-3-one. The structure of the new compound was identified as isopimar-8(14)-en-16-hydroxy-15-one. These structures were determined from extensive spectroscopic data analysis. The isolates were screened for antibacterial activity using the agar dilution method against ten test bacterial strains ( Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Micrococcus kristinae, Pseudomonas aeruginosa, Salmonella pooni, Serratia marcescens, Staphylococcus aureus, S. epidermidis and Streptococcus pyrogens). Isopimar-8(14)-en-16-hydroxy-15-one exhibited activity, with MIC values of 0.5 mg/mL against Streptococcus pyrogens; 0.25 mg/mL against Salmonella pooni and 0.1 mg/mL against Bacillus cereus, Staphylococcus aureus and Micrococcus kristinae.

scholarly journals STUDY ON ANTI-MICROBIAL PROPERTIES OF Enicosanthum membranifolium SINCLAIR AND Enicosanthum cupulare (KING) AIRY-SHAW

2015 ◽  
Vol 3 (1) ◽  
pp. 62
Author(s):  
Mai Efdi ◽  
Mamoru Koketsu ◽  
Kunitomo Watanabe ◽  
Nurainas
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Fraction ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Microbial Properties ◽  
Agar Dilution

 ABSTRACT n-Hexane and ethyl acetate fractions of methanol extracts of two species of Annonaceae, Enicosanthum membranifolium Sinclair and Enicosanthum cupulare (King) Airy-Shaw, were screened for antimicrobial activity against eighteen bacterial strains using agar dilution method. The ethyl acetate fraction of the two Annonaceae plants showed higher antimicrobial activities than the n-hexane fraction. The extracts of the plants tested were significantly more active against gram-positive with minimum inhibitory concentration (MICs) ranging from 0.0625 to 4 mg/mL than against gram negative bacteria (MICs >4 mg/mL). Keywords: Antibacterial activity; Enicosanthum membranifolium Sinclair; Enicosanthum cupulare (King) Airy-Shaw

scholarly journals Anti-HIV, Antitubercular and Antibacterial Activities of Novel 3-(Substituted Quinazolinylamino)-2-phenyl quinazolin-4(3H)ones

2019 ◽  
Vol 32 (2) ◽  
pp. 281-286
Author(s):  
M.T. Sulthana ◽  
K. Chitra ◽  
V. Alagarsamy
Keyword(s):  
Antibacterial Activity ◽  
Antitubercular Activity ◽  
Antibacterial Activities ◽  
Primary Amines ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
E Coli ◽  
Agar Dilution ◽  
Anti Hiv Agents ◽  
Anti Hiv

In the present study, we have synthesized a series of novel 2-phenyl-3-(substituted quinazolinylamino)quinazolin-4(3H)-ones by the reaction of 3-(substituted)-2-hydrazinoquinazoline-4(3H)-ones with 2-phenyl-3,1-benzoxazin-4-one. The starting material 3-(substituted)-2-hydrazinoquinazolin-4(3H)-ones were synthesized from various primary amines. All the synthesized compounds were screened for their antitubercular, anti-HIV and antibacterial activity against different Grampositive and Gram-negative strains by agar dilution method. Among the test compounds, 3-(4-nitrophenyl)-2-(4-oxo-2-phenylquinazolin-3(4H)-ylamino)quinazolin-4(3H)-one (BQZ6) and 3-(4-chlorophenyl)-2-(4-oxo-2-phenylquinazolin-3(4H)-ylamino)quinazolin-4(3H)-one (BQZ7) shown most potent antibacterial activity against E. coli, P. aeruginosa and S. aureus with the MIC of 3 μg/mL. The compound BQZ7 exhibited the antitubercular activity with the MIC of 25 μg/mL and anti-HIV activity with the MIC of 35.4 μg/mL against HIV1 and HIV2 and offers potential lead for further optimization and development to new antitubercular and anti-HIV agents. The results from this study confirm that the synthesized and biologically evaluated quinazolines showed promising antimicrobial, antitubercular and anti-HIV activities and are new scaffolds for antimicrobial activity

scholarly journals Isolation of antibacterial compounds from chloroform extract of neem (Azadirachta indica A. Juss.) leaves guided by bioautography

2005 ◽  
Vol 3 (2) ◽  
pp. 43-46
Author(s):  
DWI APRISTIANI ◽  
PUJI ASTUTI
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Azadirachta Indica ◽  
Inhibition Zone ◽  
Chloroform Extract ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Neem Leaves ◽  
Long Time ◽  
Vacuum Liquid Chromatography

Neem leaves (Azadirachta indica A. Juss.) have been traditionally used as antibacterial agent for a long time. Former research mentioned that neem leaves were proven to have antibacterial activity. This study was aimed to isolate antibacterial components in the chloroform extract of neem leaves guided by bioautography. Neem leaves were extracted by maceration technique. Antibacterial testing of chloroform extract was conducted by agar dilution method. Chloroform extract at the concentration of 1000 µg/mL inhibited the growth of Staphylococcus aureus but not Escherichia coli. The extract was then fractionated by Vacuum Liquid Chromatography (VLC) method, and the fractions obtained were tested for antibacterial activity. The mixture of 2 and 3 fractions which was eluted with n-hexane: ethyl acetate = 9:1 (v/v) and n-hexane: ethyl acetate = 5:1 (v/v) inhibited the growth of S. aureus at the concentration of 1000 µg/mL. TLCbioautography of the fraction showed two inhibition zone with hRf values of 43.75 and 18.75. Isolate with hRf of 18.75 has Minimum Inhibitory Concentration (MIC) value of 500 µg/mL and still contained many components such as terpenoid.

scholarly journals ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACT FROM STEM BARK OF Cinnamomum sintoc

2017 ◽  
Vol 17 (2) ◽  
pp. 77
Author(s):  
Nurdin Saidi ◽  
Hira Helwati ◽  
Lailatul Qhadariah Lubis ◽  
Muhammad Bahi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Stem Bark ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
E Coli ◽  
Paper Disk ◽  
Agar Dilution

Antimicrobial activity of methanol extract from stem bark of Cinnamomum sintoc has been evaluated against Candida albicans, Staphylococcus aureus and Escherichia coli. The extraction of compound was carried out by maceration, then isolation by column chromatograph, which yielded five (5) subfractions (A-E). Activity against fungus C. albicans, S. aureus bacteria dan E. coli using agar dilution method in paper disk. Methanol extract was not potent against antifungal activity but shows antibacterial activity with medium category. Subfraction C showed that antibacterial activity against S. aureus and E. coli with weak category, but subfractions D and E did not show any activity.

scholarly journals Antibacterial activity of rosemary, caraway and fennel essential oils

2015 ◽  
Vol 61 (4) ◽  
pp. 31-39 ◽  
Author(s):  
Paweł Kwiatkowski ◽  
Stefania Giedrys-Kalemba ◽  
Małgorzata Mizielińska ◽  
Artur Bartkowiak
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antibacterial Properties ◽  
Future Research ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
E Coli ◽  
Natural Medicine ◽  
Agar Dilution ◽  
Alternative Remedies

SummaryIntroduction:Recently, interest in essential oils used in natural medicine, has been increasing. Essential oils are still being tested for their potential uses as an alternative remedies for the treatment of many infectious diseases.Objective:The aim of the study was to evaluate antibacterial properties of commercial essential oils (rosemary, caraway and fennel) to reduce the number ofStaphylococcus aureusandEscherichia coli.Methods:The antibacterial activity of essential oils was investigated by agar dilution method.Results:The result of experiments showed that essential oils contained in microbiological media significantly reduced the number ofS. aureusandE. colicells. The best antibacterial properties possessed caraway oil: 1 mg/g forS. aureusand 10 mg/g forE. coli,weaker rosemary (5 mg/g) and fennel (20 mg/g) oils.Conclusion:Results confirmed the inhibiting effect of commercial essential oils onS. aureusandE. coliand provide a scientific ground for future research.

scholarly journals Antibacterial Activity Tests of Staphylococcus Aureus and Phytochemical Screening in Family Asteraceae, Clusiaceae, Phyllanthaceae

2020 ◽  
Vol 55 (6) ◽  
Author(s):  
Prasetyorini Djarot ◽  
Novi Fajar Utami ◽  
Noerma Veonicha ◽  
Anggita Rahmadini ◽  
Afrizal Nur Iman
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Skin Infections ◽  
Inhibitory Ability ◽  
Agar Dilution ◽  
Concentration Test ◽  
Material Extraction

Infection is a type of disease that affects many residents of developing countries, including Indonesia. One pathogenic bacteria that is quite dangerous and causes skin infections both sporadically and endemically is S aureus. Several studies intensively report that some of the Asteraceae, Clusiaceae and Phyllanthaceae family plants contain alkaloids, flavonoids, saponins and tannins that have potential as antibacterial S aureus. Therefore, this study aims to identify effective plants to deal with infections caused by S aureus bacteria. To this end, the ingredients used are the leaves of G. procumbens, E. scaber, G. mangostana, G atroviridis, G. xanthochymus, leaves and bark of A. neurocarpum. Plant material extraction was done by maceration using 90% ethanol solvent. Next, an antibacterial test was conducted, which was begun by testing the Minimum Inhibitory Concentration followed by testing the Obstacle Area Width. The Minimum Inhibitory Concentration test was carried out using the agar dilution method, and the Obstacle Area Width test was carried out with paper diffusion discs. The results showed that all the plants have potential as antibacterials, and the mangosteen leaf extract has the largest Obstacle Area Width of 5.1 mm with a concentration of extract of 30% and a fairly strong inhibitory ability.

Antibacterial Activity of Silver-Hydroxyapatite/Titania Nanoparticles on Oral Bacteria

2007 ◽  
Vol 330-332 ◽  
pp. 299-302 ◽  
Author(s):  
Juan Liao ◽  
An Chun Mo ◽  
Hong Kun Wu ◽  
Jing Chao Zhang ◽  
Yu Bao Li ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Agar Plate ◽  
Antibacterial Activities ◽  
Oral Bacteria ◽  
Titania Nanoparticles ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Bacterial Strains ◽  
Bacterial Accumulation ◽  
Agar Dilution

Since bacterial accumulation surrounding biomaterials has pathogens known to cause the biomaterials centered infection, it may be important to develop some kind of biomaterial with antibacterial activity as well as biocompatibility. This study focused on evaluating the antibacterial activity of silver-hydroxyapatite/ Titania nanoparticles (Ag-nHA/nTiO2) against oral bacteria with agar dilution method. Bacteria were seeded on agar plate containing antibacterial material with different concentrations after incubation of 48 hours. The antibacterial activity was demonstrated by MICs. The MICs of Ag-nHA/nTiO2 ranged between 1000μg/ ml and 7500μg /ml under anaerobic conditions. And it also exhibited remarkable antibacterial activity to all the aerobe bacteria (MIC≤500μg/ml). On the other hand, the antibacterial activities of Ag-nHA/nTiO2 differentiate to some extent with the bacterial strains. This Ag-nHA/nTiO2 exhibited remarkable antibacterial activity to anaerobic and aerobe bacteria. This antibacterial effect may reduce the potential for bacterial colonisation of oral biomaterials with Ag-nHA/nTiO2.

scholarly journals Synthesis and Antibacterial Activity of Highly Oxygenated 1,8-Cineole Derivatives

2008 ◽  
Vol 3 (3) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Margarita B. Villecco ◽  
Julieta V. Catalán ◽  
Marta I. Vega ◽  
Francisco M. Garibotto ◽  
Ricardo D. Enriz ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antimicrobial Effect ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Gram Negative Bacteria ◽  
Synthetic Compounds ◽  
Structure Activity ◽  
Agar Dilution ◽  
Sar Study ◽  
And Staphylococcus Aureus

Seventeen mono-, di- and trifunctionalized 1,8-cineole derivatives carrying OH, OAc, keto and lactone functions at C-5, C-8 and C-9 were synthesized from 1,8-cineole with fair to excellent yields. The antibacterial activity of these synthetic compounds against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus using the agar dilution method was examined. Lactones 1,3-dimethyl-2-oxabicyclo[2.2.2]octan-8- endo-acetyloxy-5→9-olide (15) and 1,3-dimethyl-2-oxabicyclo[2.2.2]octan-8- endo-ol-5→9-olide (16) showed the highest antibacterial activity against all the three Gram negative bacteria assayed. A structure-activity relationship (SAR) study on the oxygenated 1,8-cineole derivatives has allowed a model to be proposed for the recognition of the minimal structural requirements for the antimicrobial effect.

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