Nodule Occupancy Behaviour of Bacteriocinogenic Rhizobium spp. in Mungbean (Vigna radiata)

2020 ◽  
Author(s):  
P.K. Maan ◽  
S. Garcha ◽  
S. Sharma ◽  
G.S. Walia
Keyword(s):  
Seed Set ◽  
Growth Traits ◽  
Dry Weight ◽  
Viable Count ◽  
Indicator Strain ◽  
Nodule Occupancy ◽  
Culture Study ◽  
Plant Nitrogen ◽  
Rhizobium Spp ◽  
Number Of Seeds

A pot culture study was undertaken to determine the synergistic effect of bacteriocinogenic Rhizobium spp. (N8, S1, S6 and S13 isolated from mungbean fields) with Indicator Rhizobium spp. (mungbean biofertilizer) in terms of nodulation, plant growth traits and grain yield in mungbean (SML 668). Dual inoculation of bacteriocinogenic Rhizobium isolates N8 with indicator strain (indicator+N8) resulted significant increase in number of nodules (24.3 nn/plant), nodule index (1.36), nodule dry weight (23.4 mg/plant), dry weight of shoot (0.79 g/plant), dry weight of root (1.65 g/plant), chlorophyll content (1.34 mg/g of leaves), leghemoglobin content (3.43 mg/g of nodules), number of pods (15.9 /plant), number of seeds (13.8 /pods), seed set percent (86.73%), plant height (96 cm), yield (1.69 g/plant), nitrogen content of grains (4.02%) as compared to indicator strain alone. Nodule occupancy was assessed by comparing the total viable count of nodule microbiota, which demonstrated the domination of bacteriocin producers. Bacteriocinogenic Rhizobium N8 strain can be explored as potent bio-fertilizers along with indicator Rhizobium spp. in mungbean.

scholarly journals Mutation in the ntrR Gene, a Member of the vap Gene Family, Increases the Symbiotic Efficiency of Sinorhizobium meliloti

2001 ◽  
Vol 14 (7) ◽  
pp. 887-894 ◽  
Author(s):  
Boglárka Oláh ◽  
Erno Kiss ◽  
Zoltán Györgypál ◽  
Judit Borzi ◽  
Gyöngyi Cinege ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Pathogenic Bacteria ◽  
Sinorhizobium Meliloti ◽  
Root Nodules ◽  
Nifh Gene ◽  
Dry Weight ◽  
Nodule Occupancy ◽  
Gene Encoding ◽  
Symbiotic Efficiency ◽  
Host Interactions

In specific plant organs, namely the root nodules of alfalfa, fixed nitrogen (ammonia) produced by the symbiotic partner Sinorhizobium meliloti supports the growth of the host plant in nitrogen-depleted environment. Here, we report that a derivative of S. meliloti carrying a mutation in the chromosomal ntrR gene induced nodules with enhanced nitrogen fixation capacity, resulting in an increased dry weight and nitrogen content of alfalfa. The efficient nitrogen fixation is a result of the higher expression level of the nifH gene, encoding one of the subunits of the nitrogenase enzyme, and nifA, the transcriptional regulator of the nif operon. The ntrR gene, controlled negatively by its own product and positively by the symbiotic regulator syrM, is expressed in the same zone of nodules as the nif genes. As a result of the nitrogen-tolerant phenotype of the strain, the beneficial effect of the mutation on efficiency is not abolished in the presence of the exogenous nitrogen source. The ntrR mutant is highly competitive in nodule occupancy compared with the wild-type strain. Sequence analysis of the mutant region revealed a new cluster of genes, termed the “ntrPR operon,” which is highly homologous to a group of vap-related genes of various pathogenic bacteria that are presumably implicated in bacterium-host interactions. On the basis of its favorable properties, the strain is a good candidate for future agricultural utilization.

scholarly journals Influence of bee pollination on quality and quantity of onion (Allium cepa L.) seed

2020 ◽  
Vol 13 (2) ◽  
pp. 200-204
Author(s):  
Subhash B. Kandakoor ◽  
Jitendra Kumar S. Hilli
Keyword(s):  
Allium Cepa ◽  
Seed Weight ◽  
Seed Set ◽  
Maximum Activity ◽  
Major Contributor ◽  
Flowering Stage ◽  
Bee Pollination ◽  
Allium Cepa L ◽  
Bee Colonies ◽  
Number Of Seeds

The activity of bees pollination in onion was observed throughout the day. Numerically maximum activity was observed during afternoon hours (12.00 to 2.00 PM) with 7.00 numbers in rock bee, 5.40 in Indian bees, 11.60 in little bees and 7.80 in case of dammer be, respectively. Among the four species of bees, little bees were more in entire day with highest of 111.60 bees/10 inflorescence/ minute followed by dammer bee with 9.40 bees/10 inflorescence/ minute, Among the bees major contributor was little bee, this may be due to more colonies of little bees in that area and destruction of rock bee colonies. Also, the bee acitivty was observed maximum number at 100 per cent flowering stage. The observations on number of seeds per umbel, 1000 seed weight and per cent germination under the laboratory conditions. The results clearly indicated that, maximum number of seeds per umbel was observed in open pollinated flowers with 339.30 ± 60.27 seeds per umber where all the four species of bees were made visits regularly followed by in case of pollination in mesh cloth cage with bees with 330.00±35.80 and very least number of seed set was observed in case of pollination in mesh cloth cage without bees with only 60.70 seeds/umbel. Similarly, 1000 seed weight also differed significantly in case of pollination with bees and without bees. In case of with bees it weighed around 3.37 g/1000 seeds followed by 3.10 g/1000 seeds in onion with bee cage and least in case of control with only 1.97 g/1000 seeds.

scholarly journals RESPONSE OF SOME WHEAT GROWTH TRAITS FOR FOR FOLIAR SPRAYING WITH HUMIC AND GLUTAMIC ACID

2019 ◽  
Vol 50 (6) ◽  
Author(s):  
Baqir & Zeboon
Keyword(s):  
Growth Rate ◽  
Humic Acid ◽  
Glutamic Acid ◽  
Growth Traits ◽  
Flag Leaf ◽  
Block Design ◽  
Dry Weight ◽  
Engineering Sciences ◽  
Significant Difference ◽  
Two Factors

A field experiment was conducted at the Agricultural Experiment Unit, College of Agriculture Engineering Sciences, University of Baghdad, during two winter seasons 2016-2017 and 2017-2018 to study the response of some growth traits for wheat Cv. Al forat to foliar spraying using with humic glutamic acid and acid. A factorial experiment was with in Randomized Complete Block Design applied three replications, it involved two factor ,first factor was glutamic acid with three concentrations (0,250,500) mg L-1, second factor was humic acid with three concentrations (0,1,2) ml L-1, have been sprayed at tillering and flowering stages. The results showed that all the studed growth traits (plant height, ,number of tillers, flag leaf area dry weight for plant , crop growth rate ,relative growth rate and biological yield) were affected  by spraying with humic acid and glutamic acid for two season concentration , 2ml -1 from humic acid was superior on most of studies traits , as for glutamic acid ,plants treated with concentrations 250 and 500 mg L-1 were produced the highest mean for studies traits but without significant difference between them in some traits .The interaction between two factors was significant on most studies growth traits .

Effect of Shade on Giant Foxtail

Weed Science ◽  
1972 ◽  
Vol 20 (6) ◽  
pp. 588-592 ◽  
Author(s):  
Ellery L. Knake
Keyword(s):  
Dry Matter ◽  
Morphological Characteristics ◽  
Dry Weight ◽  
Field Studies ◽  
Giant Foxtail ◽  
Setaria Faberii ◽  
Number Of Leaves ◽  
Number Of Stems ◽  
Total Dry Weight ◽  
Number Of Seeds

Field studies were conducted with giant foxtail(Setaria faberiiHerrm.) under shade intensities of 0, 30, 60, 70, 80, and 98%. Seed weight, dry weight of plant tops exclusive of seed, and total dry weight per plant decreased linearly with increasing shade intensities. These decreases were due primarily to decreases in number of leaves, number of stems per plant, and number of heads per plant. Height of main culm was less affected than other morphological characteristics. Shading affected the length of internodes but had little influence on number of internodes on the main culm. The amount of shade required to control giant foxtail completely, once it is established, appears to be above 95%. Expressed as 2-year means, plants grew to as much as 135 cm, had as many as 188 leaves, 41 stems, and 31 heads, and produced 73 g of dry matter per plant including 6 g of seed. Maximum number of seeds per head was 1405.

Genetic Variation in Chamaecyparis nootkatensis from Coastal British Columbia1

1992 ◽  
Vol 7 (1) ◽  
pp. 25-29 ◽  
Author(s):  
M. L. Cherry ◽  
D. T. Lester
Keyword(s):  
Genetic Variation ◽  
Growth Traits ◽  
Weight Ratio ◽  
Common Garden ◽  
Dry Weight ◽  
Frost Damage ◽  
Chamaecyparis Nootkatensis ◽  
Shoot Dry Weight ◽  
Lateral Branches ◽  
Port Orford Cedar

Abstract Genetic variation in several traits of Alaska-cedar (Chamaecyparis nootkatensis) was measured in progeny from seven coastal B.C. provenances, each represented by three open-pollinated families. The common-garden nursery trial also included one provenance of Port-Orford cedar (Chamaecyparis lawsoniana). Growth traits and frost hardiness were measured at the end of the first growing season, and morphological traits after the second year. Provenances varied in percentage germination, shoot dry weight, shoot to root dry weight ratio, number of lateral branches per stem, and cold injury to a minimum temperature of -20°C in December. Families differed in height, root collar diameter, and in the number of nodes of primary foliage. Port-Orford cedar exhibited much greater height, stem caliper, and dry weight than any Alaska-cedar provenance, but was more susceptible to frost damage. West. J. Appl. For. 7(1):25-29.

scholarly journals Effect Of Rhizobium Inoculant On Nodulation, Yield And Yield Traits Of Chickpea (Cicer Arietinum L.) In Four Different Soils Of Greater Rajshahi

1970 ◽  
Vol 6 ◽  
pp. 45-50 ◽  
Author(s):  
Shahriar Zaman ◽  
M Abdul Mazid ◽  
Golam Kabir
Keyword(s):  
Organic Matter ◽  
Soil Fertility ◽  
Plant Height ◽  
Cicer Arietinum ◽  
Growth Analysis ◽  
Dry Weight ◽  
Yield Traits ◽  
Fresh Weight ◽  
Number Of Seeds ◽  
Different Soils

This study shows that enhancement of soil fertility along with the yield of chickpea due to nodulation by the Rhizobium is possible to some extent. Data on plant height (cm) at maturity, primary branches/plant, days to flower, pods/plant, pod dry weight/plant (g), seeds/pod, seed dry weight/pod (g), 50 seeds weight (g), nodules/plant, fresh weight of nodules/plant and dry weight of nodules/plant were considered to assess the effect of nodulation in chickpea. Soils treated with Rhizobium always showed higher values than control regarding total nitrogen and organic matter (%). Campus soil showed the highest value for organic matter (%) and N content but Cholonbil soil exhibited the highest value for number of seeds per pod. All the parameters for growth analysis yielded greater values for treated soils compared to that of the control. DOI: http://dx.doi.org/10.3329/jles.v6i0.9720 JLES 2011 6: 45-50

Effect of Vineyard Row Orientation on Growth and Phenology of Glyphosate-Resistant and Glyphosate-Susceptible Horseweed (Conyza canadensis)

Weed Science ◽  
2011 ◽  
Vol 59 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Marisa Alcorta ◽  
Matthew W. Fidelibus ◽  
Kerri L. Steenwerth ◽  
Anil Shrestha
Keyword(s):  
Leaf Area ◽  
Growth And Development ◽  
Dry Weight ◽  
Conyza Canadensis ◽  
Light Penetration ◽  
Low Light ◽  
Weed Growth ◽  
Light Levels ◽  
East West ◽  
Number Of Seeds

Horseweed has become increasingly common and difficult to control in San Joaquin Valley vineyards, due in part, to the evolution of glyphosate resistance. The development of weed-suppressive vineyard designs in which the trellis design, spacing, and row orientation combine to cast dense shade on the weed canopy zone (WCZ) may reduce weed growth. The relevance of such a system to horseweed, which can grow to be as tall, or taller, than a typical grapevine trellis, is uncertain. Also unknown is whether a glyphosate-resistant (GR) biotype and glyphosate-susceptible (GS) biotype would perform similarly under such conditions. Therefore, we compared the growth and development of two potted horseweed biotypes (GR and GS) in vinerows oriented east–west (EW) and north–south (NS). Rows oriented EW allowed less light penetration to the WCZ than NS rows throughout the study, and horseweed biotypes responded to low light levels by producing leaves with larger specific leaf area and leaf area ratios than those in the NS rows. Also, the leaf, stem, and root dry weight of the horseweed plants in the EW rows was reduced by 30% compared to the horseweed plants in NS rows. Leaf number was also reduced in the horseweed plants in the EW rows, but only for the GS biotype. Row orientation did not affect phenological development or the number of seeds produced by the GR or GS biotypes, but the GR biotype budded, flowered, and set seed approximately 1 wk earlier than the GS biotype. Thus, shade associated with the EW vinerows reduced horseweed growth, but not fecundity, and the GR biotype reached reproductive maturity earlier than the GS biotype.

Pollination and Seed Set of Banksia spinulosa: Evidence for Autogamy

1988 ◽  
Vol 36 (6) ◽  
pp. 633 ◽  
Author(s):  
G Vaughton
Keyword(s):  
New England ◽  
Breeding System ◽  
Seed Weight ◽  
National Park ◽  
Fruit Set ◽  
Seed Set ◽  
Pollen Vectors ◽  
Specific Variation ◽  
Number Of Seeds

The breeding system of Banksia spinulosa var. neoanglica in the New England National Park was examined by excluding pollen vectors and hand pollinating inflorescences. Fruit set of xenogamous and open inflorescences was greater than geitonogamous and autogamous treatments, although not all differences were significant. No differences were found in the number of seeds per follicle or seed weight between treatments. A reduced number of follicles and smaller seeds occurred in the apical third of inflorescences in all treatments. This report of autogamous seed set contrasts with previous findings for this species and indicates intra-specific variation in the breeding system.

What determines the number of seed produced in a flowering event? A case study of Calyptrogyne ghiesbreghtiana (Arecaceae)

2000 ◽  
Vol 48 (5) ◽  
pp. 659 ◽  
Author(s):  
Saul A. Cunningham
Keyword(s):  
Resource Allocation ◽  
Seed Production ◽  
Natural Variation ◽  
Seed Set ◽  
Plant Seed ◽  
Flower Production ◽  
Number Of Seeds ◽  
Female Flowers

Calyptrogyne ghiesbreghtiana Linden ex. H. Wendl. is an understorey palm that occurs at La Selva Biological Station, Costa Rica. In this paper I combine analysis of natural variation and results of experimental manipulations to ask (1) what limits seed production? and (2) what processes cause variation in seed production by C. ghiesbreghtiana? The number of seed produced per inflorescence ofC. ghiesbreghtiana was limited substantially by the loss of female flowers to floral herbivores. Much variation in the number of seed produced perinflorescence of C. ghiesbreghtiana was due to losses of developing fruits to predators. Together these phenomena illustrate the influential role of natural enemies in the reproductive success of this plant. Seed-set efficiency (number of seeds/number of female flowers) was highly variable in this species, and such high levels of variation appear to be common in other species. For species with highly variable seed-set efficiency, the role of resource allocation to flower production in determining differences in seed production among individual plants is likely to be relatively small.

Growth and reproduction of American ginseng (Panax quinquefolius) in Wisconsin, U.S.A.

1982 ◽  
Vol 60 (12) ◽  
pp. 2692-2696 ◽  
Author(s):  
Susan G. Carpenter ◽  
Grant Cottam
Keyword(s):  
Reproductive Success ◽  
Seed Set ◽  
Panax Quinquefolius ◽  
Natural Forests ◽  
Male And Female ◽  
Plant Stage ◽  
Number Of Leaves ◽  
Wild Ginseng ◽  
Number Of Seeds ◽  
Growth And Reproduction

The phenology and development of southern Wisconsin populations of wild ginseng (Panax quinquefolius L.) are described. In natural forests, ginseng plants grow slowly, and most plants do not reach reproductive maturity until they are at least 8 years old. Ginseng flowers are perfect, but maturity of male and female reproductive parts is separated temporally in each flower. Ginseng individuals are not obligate outcrossers, and the flowers are visited by generalist pollinators. Exclusion of pollinators did not reduce fruit or seed set. Aging techniques usually underestimate age. Number of leaves (plant stage) is a more appropriate indicator of development than is the number of "annual" stem scars on the rhizome. The number of seeds produced by a plant may be predicted from the number of leaves and the leaf area of the largest leaflet. Accurate estimates of the number of ginseng plants in an area can be made by sampling early in the summer. However, projections of survival and reproductive success in a ginseng population must account for mortality and for plants that senesce early in the summer months before producing seeds. We recommend that ginseng harvesting in Wisconsin be allowed only after September 1 to insure that fruits on mature plants are ripe.

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