scholarly journals Assessment of Soybean Resistance Level to Pod Sucking Bug Riptortus linearis F. (Hemiptera: Coreidae) based on No-choice and Free-choice Tests

Author(s):  
A. Krisnawati ◽  
K. Noerwijati ◽  
S.W. Indiati ◽  
Trustinah . ◽  
E. Yusnawan ◽  
...  

Background: The infestation of the pod sucking bug Riptortus linearis is one of the limiting factors of soybean productivity in Indonesia. The research aims were to identify the level of resistance to the pod sucking bug R. linearis on several soybean genotypes based on the no-choice test (NCT) and free-choice tests (FCT). Methods: The genetic materials used were 49 soybean genotypes. The study was conducted in the Indonesian Legumes and Tuber Crops Research Institute (ILETRI) from August to December 2020, using the randomized block design in triplicates. The evaluation for pod sucking bug resistance was based on NCT and FCT. Result: The FCT resulted in two genotypes with a consistent moderately resistance level (20-40% of damage intensity) to pod sucking bug based on the pod and seed damage intensity. The NCT method resulted in five genotypes as moderately resistant (20-40% of damage intensity) to pod sucking bug based on the seed damage. The NCT resulted in higher average intensity of pod and seed damage (80.25% and 71.23%, respectively) than the FCT (69.91% and 69.09%, respectively). Two soybean genotypes (Degra/Anjasmoro-1-559 and Anjasmoro/IAC100-2-618) with a consistent moderately resistance level could be used for cultivar improvement in the breeding program. The pod trichome density was suggested to be one of the effective morphological defenses against the pod sucking bug attack.

2019 ◽  
Vol 47 (2) ◽  
pp. 141-148
Author(s):  
Ayda Krisnawati ◽  
Marida Santi Yudha Ika Bayu ◽  
Dan Mochammad Muchlish Adie

The brown stink bug, Riptortus linearis, is the most dominant of soybean pod sucking pest in Indonesia. The objectivesof the study were to identify the resistance of soybean genotypes against brown stink bug and to determine the relationship ofagronomic characters with the intensity of pod and seed damages. Research was conducted at Balitkabi (Malang, Indonesia)from April to August 2018. Thirteen genotypes were assessed for their resistance to brown stink bug under choice and nochoicetests. A randomized block design was carry out with three replicates. The number of punctures on seed, seed and poddamages intensity on no-choice test was higher than those under choice test. Under choice test, the pod damage ranged from19.40-41.68% (average of 29.51%), and the seed damage ranged from 19.31-39.90% (average of 26.80%). Under no-choicetest, the pod damage ranged from 25.77-43.58% (average of 37.41%), and the seed damage ranged from 21.30-58.29%(average of 34.27%). The 11AB and 19BE showed consistently resistant based on pod and seed damage under the choicetest. Under no-choice-test, 19BE showed consistently resistant. The path analysis showed that an increase in the number ofseeds will possibility increase the number of punctures on the seed and then contribute to the increase of yield losses. The19BE which showed consistently resistant to brown stink bug was recommended to be further tested in the adaptation trialand released as superior variety.


2019 ◽  
Vol 19 (2) ◽  
pp. 135
Author(s):  
Marida Santi Yudha Ika Bayu ◽  
Yusmani Prayogo

Pod borer is a major pest on soybean. The objective of this study was to determine the resistance of soybean genotypes against pod borer based on choice test. The experiment was conducted in green house and laboratory of Entomology, Indonesian Legumes and Tuber Crops Research Institute, using Randomized Block Design with 16 soybean genotypes and three replicates. Planting dates was arranged such a way so as to sincronize the flowering time and pod formation of 16 soybean genotypes. A pairs of 4 days emerged adult pod borer were infested into each plant at R4 stage (21 days after flowering) for two days. The number of egg was observed at 2 days after infestation (DAI) and the number of larva and damaged intensity were observed at 14 DAI. The results showed that genotypes significantly affected egg and larval population, pod and seed damage intensity. The lowest egg and larval population found on Anjasmoro, 6,33 eggs and 10 individuals, respectively. The lowest intensity of pod damage found on Anjasmoro (27,74%), and the lowest intensity of seed damage found on Malabar/Sinabung-68 (15,61%). The resistance of soybean genotypes was non-preference as place to lay eggs and as feed. In conclusion, there were two genotypes showed consistently resistant (Anjasmoro and Malabar/Sinabung-68). These genotypes could be used as a source of genes for varietal improvement of soybean resistance against pod borer.


Author(s):  
Ayda Krisnawati ◽  
Marida Santi Yudha Ika Bayu ◽  
Moch. Muchlish Adie

<p>Soybean pod damaged by pod sucking bug (<em>Riptortus linearis</em>) is one of the constraint within soybean yield improvement in Indonesia. The research aimed was to identify the resistance of soybean genotypes to pod sucking pest. The experiment was conducted in Iletri’s screen house from March to June 2015. The experiment was arranged in Randomized Completely Block Design using 10 soybean genotypes with three replicates. The soybean resistance was evaluated using no-choice test. Data collected on number of pod/plant, number of seed/plant, seed weight per plant, number of attacked pod/plant, and number of attacked seed/plant. The result showed that the lowest percentage both of pod and seed damage was G511H/Anjasmoro//Anjasmoro-2-8, that was 25.83 % and 19.12 %, respectively. Based on the value of the percentage of seed damage, there were five susceptible genotypes, three moderately resistant, and two resistant genotypes. Based on the value of the percentage of pod damage, showed four susceptible genotypes, five moderately resistant, and a resistant genotype. G511H/Anjasmoro//Anjasmoro-2-8 was the only resistant genotype, and it could be used as a genetic source in the improvement of soybean resistance to pod sucking bug.</p><p><strong>How to Cite</strong></p><p>Krisnawati, A., Bayu, M. S. Y. I. &amp; Adie, M. M. (2016). Identification of Soybean Resistance to Pod Sucking Bug (<em>Riptortus linearis</em>) by No-Choice Test. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 407-414. </p>


2021 ◽  
Vol 29 ◽  
pp. 169-178
Author(s):  
Gilmar Oliveira Santos ◽  
Samuel Leandro Soares ◽  
Gustavo André Simon ◽  
Renata Cristina Alvares

Soybean crop yield is affected in the absence of favorable conditions for its complete development, and one of the most limiting factors is the water availability throughout the crop cycle. Therefore, the objective of this study was to evaluate the behavior of three soybean genotypes, in two sowing dates, during two harvests, in the city of Rio Verde, Stat of Goiás. Four experiments were carried out in a randomized block design, with four replicates in the 2018/2019 and 2019/2020 harvests. The variables analyzed in the experiment were plant height at maturity, days to maturity, and grain yield. The water balance in the soil considered the water storage of 46.8 mm. It was observed periods with greater water deficiency (53.5 mm) in the 2018/2019 harvest for all genotypes. In short, for genotypes with 100 to 110 days of the cycle, the sowing time that showed to be the most promising to obtain higher yields, was between October 20 and November 20, for genotypes with a cycle of 120 days. This range of adaptation becomes broader, mainly because these genotypes have a longer vegetative period, being, therefore, the period of least water requirement for the crop.


Author(s):  
APRI SULISTYO ◽  
ALFI INAYATI

Sulistyo A, Inayati A. 2016. Mechanisms of antixenosis, antibiosis, and tolerance of fourteen soybean genotypes in response to whiteflies (Bemisia tabaci). Biodiversitas 17: 447-453. The attack of whiteflies (Bemisia tabaci) in soybean cultivation in Indonesia is one of the limiting factors in increasing the national soybean production. Planting resistant varieties could reduce yield losses due to the damage caused by these pests. This study was conducted to evaluate the resistance of 14 soybean genotypes to the whiteflies. A free- choice and no-choice test was conducted in a green house to study the antixenosis and antibiosis. Meanwhile, field evaluation was conducted to determine the tolerance of soybean genotypes to the whiteflies. Determination of the resistance of soybean genotypes to whiteflies based on the intensity of leaf damage that occurs on fifth weeks after infestation. The results showed that in free-choice test, Gema, IAC-100/Kaba-6, Malabar/IAC-100-85, Kaba/IAC-100//Burangrang-60, and Kaba/IAC-100//Burangrang-63 showed antixenosis mechanism which correlates with length and low density of leaf trichomes as well as leaf thickness. In the no-choice test, antibiosis mechanism can be seen from the small number of adults that develop from nymphs. IAC-100/Kaba-8 and IAC-100/Kaba-14 showed a high degree of antibiosis. In addition, the results of field experiment showed that Gema, IAC-100/Kaba-14, and Tanggamus/Pangrango- 78 demonstrated a tolerance to whiteflies. It is shown on a slightly decreasing in yield of these three genotypes (17.33, 19.31, and 19.85%, respectively).


2018 ◽  
Vol 24 (1) ◽  
pp. 43
Author(s):  
Syahrir Pakki ◽  
Mappaganggang Mappaganggang

<p>Two experiments to study responses of resistance of maize germplasm to downy mildew (Peronosclerospora philipinensis) have been conducted. Both experiments were arranged in randomized block design with two replication. The treatments in each experiment (2014 and 2016) were 70 maize accessions. The accessions were planted in planting distant of 75 cm × 20 cm, in 5 m rows, resulting in 20 plants per row. In every 10 rows, two check varieties were planted, i.e. one susceptible and one resistant variety. Ten days prior to planting, the soil was fertilized with mixed fertilizers consisted of urea, ZA, SP36, and KCl at a dose of 100, 100, 100, and 100 kg/ha, respectively. Fertilization II and III was given at 30 days after planting, and 45 days after planting with a dose of 100 kg urea/ha. As a source of inoculum downy mildew, around the repeated plot was planted with varieties Anoman variety (susceptible variety) that was inoculated with a suspension of downy mildew pathogen. Observation was conducted at 25, 35, and 55 days after planting, with the standard score of resistant to downy mildew, followed: 0–10% = resistant, 11–25% = moderately resistant, 26–50% = moderately susceptible, &gt;50% = susceptible. The result of the experiments showed that there were 5 accessions resistant to downy mildew (P. philipinensis), i.e. CML 440×MR4-9-30-3, 664, 60, 572, and 554 with the intensity of downy mildew infection ranged from 5 to 10%. Twelve other accessions were classified as moderately resistant, i.e. CML 440×MR4-9-98-2, 440×MR4-9-98-4 CML, CML 440×MR4-9-124-1, 66, 71, 319, 108, 73, 48, 105, 554, and 682 with intensity of infection ranged from 13 to 25%. At the same experiments, the average intensity of downy mildew infection on susceptible check varieties reached 100 percent.</p>


Agrikultura ◽  
2018 ◽  
Vol 29 (1) ◽  
pp. 35
Author(s):  
N. Usyati ◽  
Nia Kurniawati ◽  
Ade Ruskandar ◽  
Oco Rumasa

ABSTRACTPest and natural enemy population in three different rice cultivation in Sukamandi RegionSome on limiting factors in rice production include the cultivation system and pest damage. To suppress the damage, several control techniques have been applied, such as technical culture. The aim of this study was to gain information on population and pest damage, as well as natural enemy population in three different rice cultivation systems. The study was arranged in Randomized Block Design with three treatment and 9 replications. The treatments were: 1) organic rice cultivation, 2) semi organic, and 3) farmer technique. The used rice variety was Inpari 30. The plot size was 6 m x 90 m. The variables observed included population and pest damage, natural enemy population, and rice yields. Thirthy two rice hills were observed randomly in diagonal direction, with 2 weeks interval from two weeks after transplanting until harvest. The data were analyzed by analysis of variance (Anova) and the difference among the treatments was evaluated with Duncan multiple area test at 5% level. The results showed that brown plant hoppers population on organic rice cultivation is lower than semi-organic rice cultivation and farmer technique, but there were no difference of natural enemy population among treatments. The lowest yield was obtained from the organic rice cultivation (2.67 t/ha).Keywords: Rice cultivation, Pests, Natural enemiesABSTRAKBeberapa faktor pembatas produksi padi diantaranya adalah cara budidaya dan adanya serangan hama. Untuk menekan serangan hama, beberapa teknik pengendalian telah diterapkan diantaranya adalah pengendalian secara kultur teknis (cara budidaya). Pada MT-2 tahun 2016, penelitian dengan tujuan mendapatkan informasi mengenai populasi dan serangan hama, serta populasi musuh alami pada tiga cara budidaya padi telah dilakukan di lahan kebun percobaan Balai Besar Penelitian Tanaman Padi Sukamandi. Rancangan yang digunakan adalah Rancangan Acak Kelompok (RAK) dengan tiga cara budidaya dan diulang sebanyak sembilan kali. Adapun cara budidaya yang digunakan terdiri atas:1) budidaya padi organik; 2) semi organik; 3) cara petani. Varietas yang digunakan adalah Inpari 30. Ukuran plot 6 m x 90 m. Variabel yang diamati meliputi populasi dan tingkat serangan hama, populasi musuh alami, dan hasil panen. Pengamatan dilakukan secara langsung di pertanaman pada 32 rumpun sampel secara acak diagonal dengan interval dua minggu sekali mulai umur tanaman dua minggu setelah tanam sampai menjelang panen. Data yang diperoleh dianalisis dengan analisis ragam (Anova) dan perbedaan antar perlakuan dievaluasi dengan uji wilayah berganda Duncan pada taraf 5%. Hasil penelitian menunjukkan bahwa populasi hama wereng coklat pada cara budidaya padi organik lebih rendah dibandingkan cara budidaya padi semi organik dan budidaya padi cara petani, tetapi tidak ada perbedaan populasi musuh alami pada cara budidaya padi organik, cara budidaya padi semi organik dan budidaya padi cara petani. Hasil panen terendah (2,67 t/ha) terlihat pada perlakuan budidaya padi organik.Kata Kunci: Budidaya padi, Hama, Musuh alami


Agrikultura ◽  
2018 ◽  
Vol 29 (2) ◽  
pp. 100
Author(s):  
Helmi Kurniawan ◽  
Ineu Sulastrini ◽  
Tarkus Suganda

ABSTRACTResistance Test of Potato Clones Derived from Crossing of Atlantic x Repita to Late Blight (Phytophthora infestans)Late blight, incited by Phytophthora infestans is the most destructive disease of potato. The management that is effective and environmentally-friendly is the use of resistant variety. The objective of this study was to test the resistance of the six potato clones (AR 04, AR 05, AR 06, AR 07, AR 08 and AR 09) derived from crossing var. Atlantic x var. Repita to late blight caused by P. infestans. Var. Atlantic, Repita and Granola were used as susceptible, resistant and susceptible but the most-grown variety, respectively. Field test was located in Ciwidey, one of the potato growing center where late blight is endemic since potatoes are continuously grown. The treatments were arranged in a randomized block design with 3 replicates. The result showed that clones AR 07 and AR 08 were more resistant than the other potato clones, but it still below the resistance level var. Repita. However, based on statistical test on the diseases development (AUDPC), clone AR 08 could be categorized as resistant, equal with of the resistance level of var. Repita.Keywords: Potato clones, Rsistance, P. infestansABSTRAKPenyakit hawar daun yang disebabkan Phytopthora infestans merupakan penyakit utama pada tanaman kentang. Pengendalian yang efektif dan ramah lingkungan adalah dengan penanaman varietas tahan. Penelitian ini bertujuan untuk menguji ketahanan 6 klon kentang (AR 4, AR5, AR6, AR7, AR 8, dan AR9) yang merupakan hasil persilangan antara var. Atlantic (produksi tinggi tetapi rentan) dengan var. Repita, sebagai tetua tahan terhadap penyakit hawar daun yang disebabkan oleh P. infestans. Varietas Atlantic, Repita dan Granola digunakan sebagai pembanding. Pengujian ketahanan dilakukan di Ciwidey, yang merupakan salah satu sentra produksi kentang di Jawa Barat dan endemik penyakit hawar daun. Perlakuan ditata menggunakan Rancangan Acak Kelompok dengan 3 ulangan. Hasil penelitian menunjukkan bahwa klon AR 07 dan Klon AR 08 memiliki ketahanan yang lebih baik dibanding klon-klon kentang lainnya, namun tingkat ketahanannya masih di bawah cv Repita. Namun, berdasarkan uji statistik terhadap nilai perkembangan penyakit (AUDPC) klon AR 08 dapat dikategorikan tahan, sama dengan derajat tahan var. Repita.Kata Kunci: Klon kentang, Ketahanan, P. infestans


Revista CERES ◽  
2013 ◽  
Vol 60 (5) ◽  
pp. 621-628 ◽  
Author(s):  
Gederson Luiz Buzzello ◽  
Michelangelo Muzell Trezzi ◽  
José Abramo Marchese ◽  
Elouize Xavier ◽  
Edemir Miotto Junior ◽  
...  

Soybean genotypes grown in sub-tropical climate may exhibit lodging. The plant lodging is influenced by soil type and fertility level, sowing date, latitude and altitude of the location, plant population and conditions of crop development. Plant regulators and herbicides are able to avoid or reduce plant lodging. This study aimed to verify the effects of the growth regulators TIBA and daminozide on vegetative growth and yield of soybean cultivar CD 214 RR. The experiment was carried out at a field in randomized block design with four replications in a factorial scheme. The A factor was represented by the combination of regulators TIBA and daminozide and its concentrations, and the Factor B was seven times of evaluation of injury and plant height or eight times of evaluation of lodging. In the range of doses used, the application of daminozide resulted in greater injury to soybean plants than TIBA. The smaller plant height was achieved by the application of 6 g ha-1 of TIBA and 1200 g ha-¹ of daminozide. Treatments with daminozide (100 g ha-¹) and TIBA (10 g ha-1) stood out due to the reduced lodging of soybean plants. Grain weight increased linearly when the levels of TIBA increased. There was a negative correlation between lodging and grain yield and a positive correlation between plant height and lodging. There was also a negative correlation between injury caused by the application of plant regulators and lodging.


2017 ◽  
Vol 41 (4) ◽  
pp. 439-446 ◽  
Author(s):  
Guilherme Vieira Pimentel ◽  
Adriano Cirino Tomaz ◽  
Bruno Portela Brasileiro ◽  
Luiz Alexandre Peternelli ◽  
Márcio Henrique Pereira Barbosa

ABSTRACT The sugarcane borer Diatraea saccharalis Fabr. (Lepidoptera: Crambidae) is one of the most important pests of sugarcane in the Americas. The purpose of this study was to assess multiple resistance traits in eight sugarcane genotypes against D. saccharalis, including five commercial cultivars and three exotic germplasm with potential to serve as resistance sources against this pest. The oviposition preference was assessed by using both free-choice and non-choice tests. The performance of both early stage larvae feeding on the leaves and late stages larvae feeding within the stalks were also assessed. There were differences among genotypes for number of both eggs and egg cluster in the free-choice test while no differences in non-choice test were observed. There were also differences in survival of early stage larvae feeding on the leaves, foliar injury rating and stalk damage. The genotype IM76-228 was the least preferred for oviposition and it seems that leaf width had some influence on adults’ preference rather than greening of the leaves. IM76-228 and RB867515 causes higher mortality of early stage larvae feeding in the stalks while IM76-228 and RB985523 had lower damage in both leaves and stalks. The genotype IM76-228 was the most resistant to D. saccharalis and could serve as genes sources for resistance in sugarcane breeding programs.


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